PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA

Metagenomics is providing conspicuous advantage to explore world of unculturable microorganisms in the natural samples to enhance our information about bacterial diversity. Here, we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing tec...
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Autor*in:	SAMY SELIM [verfasserIn] NASHWA HAGAGY [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Französisch

Erschienen:	2018

Schlagwörter:	we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae Siphoviridae and unclassified phages. Moreover a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area Freshwater Lake functional genes Metagenomics microbial diversity 454 pyrosequencing

Übergeordnetes Werk:	In: Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry - Alma Mater Publishing House "Vasile Alecsandri" University of Bacau, 2010, 19(2018), 1, Seite 91-96
Übergeordnetes Werk:	volume:19 ; year:2018 ; number:1 ; pages:91-96

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Katalog-ID:	DOAJ056062419

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650		4	\|a we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae
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spelling	(DE-627)DOAJ056062419 (DE-599)DOAJd3506508666d48098a2bb0a614fbfe46 DE-627 ger DE-627 rakwb eng fre TP1-1185 SAMY SELIM verfasserin aut PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Metagenomics is providing conspicuous advantage to explore world of unculturable microorganisms in the natural samples to enhance our information about bacterial diversity. Here, we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae, Siphoviridae and unclassified phages. Moreover, a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area. we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae Siphoviridae and unclassified phages. Moreover a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area Freshwater Lake functional genes Metagenomics microbial diversity 454 pyrosequencing Chemical technology NASHWA HAGAGY verfasserin aut In Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry Alma Mater Publishing House "Vasile Alecsandri" University of Bacau, 2010 19(2018), 1, Seite 91-96 (DE-627)685778495 (DE-600)2651661-5 1582540X nnns volume:19 year:2018 number:1 pages:91-96 https://doaj.org/article/d3506508666d48098a2bb0a614fbfe46 kostenfrei http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201801&vol=1&aid=4694 kostenfrei https://doaj.org/toc/1582-540X Journal toc kostenfrei https://doaj.org/toc/1582-540X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 1 91-96
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allfieldsSound	(DE-627)DOAJ056062419 (DE-599)DOAJd3506508666d48098a2bb0a614fbfe46 DE-627 ger DE-627 rakwb eng fre TP1-1185 SAMY SELIM verfasserin aut PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Metagenomics is providing conspicuous advantage to explore world of unculturable microorganisms in the natural samples to enhance our information about bacterial diversity. Here, we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae, Siphoviridae and unclassified phages. Moreover, a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area. we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae Siphoviridae and unclassified phages. Moreover a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area Freshwater Lake functional genes Metagenomics microbial diversity 454 pyrosequencing Chemical technology NASHWA HAGAGY verfasserin aut In Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry Alma Mater Publishing House "Vasile Alecsandri" University of Bacau, 2010 19(2018), 1, Seite 91-96 (DE-627)685778495 (DE-600)2651661-5 1582540X nnns volume:19 year:2018 number:1 pages:91-96 https://doaj.org/article/d3506508666d48098a2bb0a614fbfe46 kostenfrei http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201801&vol=1&aid=4694 kostenfrei https://doaj.org/toc/1582-540X Journal toc kostenfrei https://doaj.org/toc/1582-540X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 1 91-96
language	English French
source	In Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry 19(2018), 1, Seite 91-96 volume:19 year:2018 number:1 pages:91-96
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topic_facet	we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae Siphoviridae and unclassified phages. Moreover a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area Freshwater Lake functional genes Metagenomics microbial diversity 454 pyrosequencing Chemical technology
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author	SAMY SELIM
spellingShingle	SAMY SELIM misc TP1-1185 misc we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae misc Siphoviridae and unclassified phages. Moreover misc a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area misc Freshwater Lake misc functional genes misc Metagenomics misc microbial diversity misc 454 pyrosequencing misc Chemical technology PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA
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topic_title	TP1-1185 PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae Siphoviridae and unclassified phages. Moreover a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area Freshwater Lake functional genes Metagenomics microbial diversity 454 pyrosequencing
topic	misc TP1-1185 misc we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae misc Siphoviridae and unclassified phages. Moreover misc a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area misc Freshwater Lake misc functional genes misc Metagenomics misc microbial diversity misc 454 pyrosequencing misc Chemical technology
topic_unstemmed	misc TP1-1185 misc we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae misc Siphoviridae and unclassified phages. Moreover misc a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area misc Freshwater Lake misc functional genes misc Metagenomics misc microbial diversity misc 454 pyrosequencing misc Chemical technology
topic_browse	misc TP1-1185 misc we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae misc Siphoviridae and unclassified phages. Moreover misc a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area misc Freshwater Lake misc functional genes misc Metagenomics misc microbial diversity misc 454 pyrosequencing misc Chemical technology
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title	PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA
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title_sort	pyrosequencing and functional annotation analysis of microbial community in fresh water lake in saudi arabia
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title_auth	PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA
abstract	Metagenomics is providing conspicuous advantage to explore world of unculturable microorganisms in the natural samples to enhance our information about bacterial diversity. Here, we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae, Siphoviridae and unclassified phages. Moreover, a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area.
abstractGer	Metagenomics is providing conspicuous advantage to explore world of unculturable microorganisms in the natural samples to enhance our information about bacterial diversity. Here, we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae, Siphoviridae and unclassified phages. Moreover, a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area.
abstract_unstemmed	Metagenomics is providing conspicuous advantage to explore world of unculturable microorganisms in the natural samples to enhance our information about bacterial diversity. Here, we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae, Siphoviridae and unclassified phages. Moreover, a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. The great diversity of microflora present in the lake may reflect the human activity in the area.
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title_short	PYROSEQUENCING AND FUNCTIONAL ANNOTATION ANALYSIS OF MICROBIAL COMMUNITY IN FRESH WATER LAKE IN SAUDI ARABIA
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The great diversity of microflora present in the lake may reflect the human activity in the area.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">we have performed metagenomic analysis of fresh water lake with focus on bacterial community using 454 pyrosequencing techniques. Roche GS FLX software indicated total of 156.253 reads; 15.226 contigs having < 100 bp sequence length whereas 10.481 contigs with < 500 bp sequence length. We have analyzed the bacterial community composition using BLASTN/BLASTX against NT/NR databases with E-value cutoff of 10-5. We have observed a wide array of bacteria from phylum proteobacteria and family Enterobacteriaceae as well as very few viruses from Podoviridae</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Siphoviridae and unclassified phages. Moreover</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">a functional analysis of all 5974 contigs using Rapid Annotation by Subsystem Technology (RAST) was performed and detected 15.319 coding sequences and 197 RNAs in 619 subsystems. 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