EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma

Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Memon AA [verfasserIn] Zhang HP [verfasserIn] Gu Y [verfasserIn] Luo Q [verfasserIn] Shi JJ [verfasserIn] Deng Z [verfasserIn] Ma J [verfasserIn] Ma W [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors.

Übergeordnetes Werk:	In: OncoTargets and Therapy - Dove Medical Press, 2009, (2017), Seite 4607-4613
Übergeordnetes Werk:	year:2017 ; pages:4607-4613

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ056154321

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allfields	(DE-627)DOAJ056154321 (DE-599)DOAJ9971e369c0fe4167802ab6d54090b04d DE-627 ger DE-627 rakwb eng RC254-282 Memon AA verfasserin aut EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Zhang HP verfasserin aut Gu Y verfasserin aut Luo Q verfasserin aut Shi JJ verfasserin aut Deng Z verfasserin aut Ma J verfasserin aut Ma W verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2017), Seite 4607-4613 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2017 pages:4607-4613 https://doaj.org/article/9971e369c0fe4167802ab6d54090b04d kostenfrei https://www.dovepress.com/egfr-with-tki-sensitive-mutations-in-exon-19-is-highly-expressed-and-f-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2017 4607-4613
spelling	(DE-627)DOAJ056154321 (DE-599)DOAJ9971e369c0fe4167802ab6d54090b04d DE-627 ger DE-627 rakwb eng RC254-282 Memon AA verfasserin aut EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Zhang HP verfasserin aut Gu Y verfasserin aut Luo Q verfasserin aut Shi JJ verfasserin aut Deng Z verfasserin aut Ma J verfasserin aut Ma W verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2017), Seite 4607-4613 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2017 pages:4607-4613 https://doaj.org/article/9971e369c0fe4167802ab6d54090b04d kostenfrei https://www.dovepress.com/egfr-with-tki-sensitive-mutations-in-exon-19-is-highly-expressed-and-f-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2017 4607-4613
allfields_unstemmed	(DE-627)DOAJ056154321 (DE-599)DOAJ9971e369c0fe4167802ab6d54090b04d DE-627 ger DE-627 rakwb eng RC254-282 Memon AA verfasserin aut EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Zhang HP verfasserin aut Gu Y verfasserin aut Luo Q verfasserin aut Shi JJ verfasserin aut Deng Z verfasserin aut Ma J verfasserin aut Ma W verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2017), Seite 4607-4613 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2017 pages:4607-4613 https://doaj.org/article/9971e369c0fe4167802ab6d54090b04d kostenfrei https://www.dovepress.com/egfr-with-tki-sensitive-mutations-in-exon-19-is-highly-expressed-and-f-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2017 4607-4613
allfieldsGer	(DE-627)DOAJ056154321 (DE-599)DOAJ9971e369c0fe4167802ab6d54090b04d DE-627 ger DE-627 rakwb eng RC254-282 Memon AA verfasserin aut EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Zhang HP verfasserin aut Gu Y verfasserin aut Luo Q verfasserin aut Shi JJ verfasserin aut Deng Z verfasserin aut Ma J verfasserin aut Ma W verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2017), Seite 4607-4613 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2017 pages:4607-4613 https://doaj.org/article/9971e369c0fe4167802ab6d54090b04d kostenfrei https://www.dovepress.com/egfr-with-tki-sensitive-mutations-in-exon-19-is-highly-expressed-and-f-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2017 4607-4613
allfieldsSound	(DE-627)DOAJ056154321 (DE-599)DOAJ9971e369c0fe4167802ab6d54090b04d DE-627 ger DE-627 rakwb eng RC254-282 Memon AA verfasserin aut EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Zhang HP verfasserin aut Gu Y verfasserin aut Luo Q verfasserin aut Shi JJ verfasserin aut Deng Z verfasserin aut Ma J verfasserin aut Ma W verfasserin aut In OncoTargets and Therapy Dove Medical Press, 2009 (2017), Seite 4607-4613 (DE-627)600307654 (DE-600)2495130-4 11786930 nnns year:2017 pages:4607-4613 https://doaj.org/article/9971e369c0fe4167802ab6d54090b04d kostenfrei https://www.dovepress.com/egfr-with-tki-sensitive-mutations-in-exon-19-is-highly-expressed-and-f-peer-reviewed-article-OTT kostenfrei https://doaj.org/toc/1178-6930 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2017 4607-4613
language	English
source	In OncoTargets and Therapy (2017), Seite 4607-4613 year:2017 pages:4607-4613
sourceStr	In OncoTargets and Therapy (2017), Seite 4607-4613 year:2017 pages:4607-4613
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors. Neoplasms. Tumors. Oncology. Including cancer and carcinogens
isfreeaccess_bool	true
container_title	OncoTargets and Therapy
authorswithroles_txt_mv	Memon AA @@aut@@ Zhang HP @@aut@@ Gu Y @@aut@@ Luo Q @@aut@@ Shi JJ @@aut@@ Deng Z @@aut@@ Ma J @@aut@@ Ma W @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	600307654
id	DOAJ056154321
language_de	englisch
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These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR&ndash;TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR&ndash;TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. 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spellingShingle	Memon AA misc RC254-282 misc Lung Squamous Carcinoma misc EGFR exon 19 misc Mutation Profile misc PCR-DGGE misc Tyrosine Kinase Inhibitors. misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma
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topic_title	RC254-282 EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma Lung Squamous Carcinoma EGFR exon 19 Mutation Profile PCR-DGGE Tyrosine Kinase Inhibitors
topic	misc RC254-282 misc Lung Squamous Carcinoma misc EGFR exon 19 misc Mutation Profile misc PCR-DGGE misc Tyrosine Kinase Inhibitors. misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc Lung Squamous Carcinoma misc EGFR exon 19 misc Mutation Profile misc PCR-DGGE misc Tyrosine Kinase Inhibitors. misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma
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title_full	EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma
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title_sort	egfr with tki-sensitive mutations in exon 19 is highly expressed and frequently detected in chinese patients with lung squamous carcinoma
callnumber	RC254-282
title_auth	EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma
abstract	Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors
abstractGer	Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors
abstract_unstemmed	Aadil Ahmed Memon,1 Haiping Zhang,2 Ye Gu,3 Qian Luo,4 Jiajun Shi,1 Zixin Deng,1 Jian Ma,5 Wei Ma1 1State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 2Oncology Department, 3Endoscope Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 4Core Facility and Technical Service Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 5Pneumology Department, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China Abstract: Recently, tyrosine kinase inhibitors (TKIs) have been recommended as a first-line treatment for advanced non-small cell lung cancer (NSCLC), significantly improving the treatment outcomes of lung adenocarcinoma patients with the EGFR mutation. However, the application of TKIs for lung squamous cell carcinoma (SCC), the second largest pathological subtype of NSCLC, remains controversial because available data for the EGFR mutation profile and frequency in SCC patients are limited. In this study, 89 bronchoscopic–biopsy specimens from Chinese SCC male patients were assayed for EGFR exon 19 mutation, using improved polymerase chain reaction-denature gel gradient electrophoresis. EGFR exon 19 mutations were detected in 77 of 89 (86.5%) patients, and included six kinds of point mutations (11.6%) and two deletions (Del_747-751 [64.9%] and Del_746-751 [23.3%]). We found that the proportion of mutated EGFR varied from 0.98% to 100% in positive specimens and increased with the development of the disease. The difference of proportion between Stage IV patients and Stage II patients or Stage III patients was significant (P<0.001). These results provided valuable clues to explain the reason why patients harboring the same mutation responded distinctly to TKI treatment. Del_747-751 and Del_746-751 were the dominant mutations in the assayed SCC patients (76.4%), and both belong to the EGFR–TKI-sensitive mutation. Recently research demonstrated that Del_746-751 patients have better response to EGFR-TKI than Del_L747-751 patients. However, our study indicated that majority of SCC patients (55.5%) carried Del_ L747-751. We suggest that the unique clinic features of SCC should be further studied to reveal the mechanism of poorer treatment outcome of EGFR–TKI therapy, and that a better treatment plan and more specific, potent targeted drugs for lung SCC need to be developed. Keywords: lung squamous carcinoma, EGFR exon 19, mutation profile, PCR-DGGE, tyrosine kinase inhibitors
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title_short	EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma
url	https://doaj.org/article/9971e369c0fe4167802ab6d54090b04d https://www.dovepress.com/egfr-with-tki-sensitive-mutations-in-exon-19-is-highly-expressed-and-f-peer-reviewed-article-OTT https://doaj.org/toc/1178-6930
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EGFR with TKI-sensitive mutations in exon 19 is highly expressed and frequently detected in Chinese patients with lung squamous carcinoma

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