Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice

Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted i...
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Autor*in:	Zhu Yu-Chao [verfasserIn] He Yong [verfasserIn] Liu Jian-Fa [verfasserIn] Chen Jia [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity

Übergeordnetes Werk:	In: Parasite - EDP Sciences, 2015, 27, p 26(2020)
Übergeordnetes Werk:	volume:27, p 26 ; year:2020

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1051/parasite/2020021

Katalog-ID:	DOAJ056539169

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520			\|a Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites.
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Indexfelder

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matchkey_str	article:17761042:2020----::duatcyoiel3mrvshpoetvimntocctiedaacnorpado1aan
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allfields	10.1051/parasite/2020021 doi (DE-627)DOAJ056539169 (DE-599)DOAJ0ebae2dba4d641af94f858a0b7733052 DE-627 ger DE-627 rakwb eng RC109-216 Zhu Yu-Chao verfasserin aut Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites. toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity Infectious and parasitic diseases He Yong verfasserin aut Liu Jian-Fa verfasserin aut Chen Jia verfasserin aut In Parasite EDP Sciences, 2015 27, p 26(2020) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:27, p 26 year:2020 https://doi.org/10.1051/parasite/2020021 kostenfrei https://doaj.org/article/0ebae2dba4d641af94f858a0b7733052 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite190121/parasite190121.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27, p 26 2020
spelling	10.1051/parasite/2020021 doi (DE-627)DOAJ056539169 (DE-599)DOAJ0ebae2dba4d641af94f858a0b7733052 DE-627 ger DE-627 rakwb eng RC109-216 Zhu Yu-Chao verfasserin aut Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites. toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity Infectious and parasitic diseases He Yong verfasserin aut Liu Jian-Fa verfasserin aut Chen Jia verfasserin aut In Parasite EDP Sciences, 2015 27, p 26(2020) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:27, p 26 year:2020 https://doi.org/10.1051/parasite/2020021 kostenfrei https://doaj.org/article/0ebae2dba4d641af94f858a0b7733052 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite190121/parasite190121.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27, p 26 2020
allfields_unstemmed	10.1051/parasite/2020021 doi (DE-627)DOAJ056539169 (DE-599)DOAJ0ebae2dba4d641af94f858a0b7733052 DE-627 ger DE-627 rakwb eng RC109-216 Zhu Yu-Chao verfasserin aut Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites. toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity Infectious and parasitic diseases He Yong verfasserin aut Liu Jian-Fa verfasserin aut Chen Jia verfasserin aut In Parasite EDP Sciences, 2015 27, p 26(2020) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:27, p 26 year:2020 https://doi.org/10.1051/parasite/2020021 kostenfrei https://doaj.org/article/0ebae2dba4d641af94f858a0b7733052 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite190121/parasite190121.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27, p 26 2020
allfieldsGer	10.1051/parasite/2020021 doi (DE-627)DOAJ056539169 (DE-599)DOAJ0ebae2dba4d641af94f858a0b7733052 DE-627 ger DE-627 rakwb eng RC109-216 Zhu Yu-Chao verfasserin aut Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites. toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity Infectious and parasitic diseases He Yong verfasserin aut Liu Jian-Fa verfasserin aut Chen Jia verfasserin aut In Parasite EDP Sciences, 2015 27, p 26(2020) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:27, p 26 year:2020 https://doi.org/10.1051/parasite/2020021 kostenfrei https://doaj.org/article/0ebae2dba4d641af94f858a0b7733052 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite190121/parasite190121.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27, p 26 2020
allfieldsSound	10.1051/parasite/2020021 doi (DE-627)DOAJ056539169 (DE-599)DOAJ0ebae2dba4d641af94f858a0b7733052 DE-627 ger DE-627 rakwb eng RC109-216 Zhu Yu-Chao verfasserin aut Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites. toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity Infectious and parasitic diseases He Yong verfasserin aut Liu Jian-Fa verfasserin aut Chen Jia verfasserin aut In Parasite EDP Sciences, 2015 27, p 26(2020) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:27, p 26 year:2020 https://doi.org/10.1051/parasite/2020021 kostenfrei https://doaj.org/article/0ebae2dba4d641af94f858a0b7733052 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite190121/parasite190121.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 27, p 26 2020
language	English
source	In Parasite 27, p 26(2020) volume:27, p 26 year:2020
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institution	findex.gbv.de
topic_facet	toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity Infectious and parasitic diseases
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container_title	Parasite
authorswithroles_txt_mv	Zhu Yu-Chao @@aut@@ He Yong @@aut@@ Liu Jian-Fa @@aut@@ Chen Jia @@aut@@
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callnumber-first	R - Medicine
author	Zhu Yu-Chao
spellingShingle	Zhu Yu-Chao misc RC109-216 misc toxoplasma gondii misc toxoplasmosis misc il-33 misc rhoptry protein 5 (rop5) misc rhoptry protein 18 (rop18) misc dna vaccine misc protective immunity misc Infectious and parasitic diseases Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
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topic_title	RC109-216 Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice toxoplasma gondii toxoplasmosis il-33 rhoptry protein 5 (rop5) rhoptry protein 18 (rop18) dna vaccine protective immunity
topic	misc RC109-216 misc toxoplasma gondii misc toxoplasmosis misc il-33 misc rhoptry protein 5 (rop5) misc rhoptry protein 18 (rop18) misc dna vaccine misc protective immunity misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc toxoplasma gondii misc toxoplasmosis misc il-33 misc rhoptry protein 5 (rop5) misc rhoptry protein 18 (rop18) misc dna vaccine misc protective immunity misc Infectious and parasitic diseases
topic_browse	misc RC109-216 misc toxoplasma gondii misc toxoplasmosis misc il-33 misc rhoptry protein 5 (rop5) misc rhoptry protein 18 (rop18) misc dna vaccine misc protective immunity misc Infectious and parasitic diseases
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title	Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
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title_full	Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
author_sort	Zhu Yu-Chao
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author_browse	Zhu Yu-Chao He Yong Liu Jian-Fa Chen Jia
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title_sort	adjuvantic cytokine il-33 improves the protective immunity of cocktailed dna vaccine of rop5 and rop18 against toxoplasma gondii infection in mice
callnumber	RC109-216
title_auth	Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
abstract	Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites.
abstractGer	Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites.
abstract_unstemmed	Toxoplasma gondii is a threat for immunocompromized individuals, and no treatment is available for enhancing immunity against infection. Molecular adjuvants may improve the efficacy of DNA vaccine-induced T cell immunity. Here, we report that cocktailed DNA immunization with ROP5 and ROP18 boosted immune responses induced by a single DNA immunization with ROP5 or ROP18, but also that co-administration of molecular adjuvant IL-33 enhanced immune efficacy induced by this cocktailed DNA vaccination. These improved immune responses were characterized by higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-γ, IL-2, IL-12, as well as cell-mediated activity with higher frequencies of CD8+ and CD4+ T cells. More importantly, this enhanced immunity has the ability to confer remarkable protection against a high dose lethal challenge of the T. gondii RH strain and thus against chronic infection with the T. gondii PRU strain. These data show that IL-33 is a promising immunoadjuvant to facilitate humoral as well as cellular immunity in a vaccine setting against T. gondii, and suggest that it should be evaluated in strategies against other apicomplexan parasites.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
title_short	Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice
url	https://doi.org/10.1051/parasite/2020021 https://doaj.org/article/0ebae2dba4d641af94f858a0b7733052 https://www.parasite-journal.org/articles/parasite/full_html/2020/01/parasite190121/parasite190121.html https://doaj.org/toc/1776-1042
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author2	He Yong Liu Jian-Fa Chen Jia
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doi_str	10.1051/parasite/2020021
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up_date	2024-07-03T21:26:50.555Z
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Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice

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