Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts

Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, le...
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Gespeichert in:

Autor*in:	Saira Tanweer [verfasserIn] Tariq Mehmood [verfasserIn] Saadia Zainab [verfasserIn] Zulfiqar Ahmad [verfasserIn] Aamir Shehzad [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Phytochemical screening In vitro analysis Quantification HPLC Gingerol

Übergeordnetes Werk:	In: Clinical Phytoscience - SpringerOpen, 2015, 6(2020), 1, Seite 12
Übergeordnetes Werk:	volume:6 ; year:2020 ; number:1 ; pages:12

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s40816-020-00158-z

Katalog-ID:	DOAJ056791208

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520			\|a Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g).
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allfields	10.1186/s40816-020-00158-z doi (DE-627)DOAJ056791208 (DE-599)DOAJ5a475f4514ca4ab4b05b4772f286feef DE-627 ger DE-627 rakwb eng RX1-681 Saira Tanweer verfasserin aut Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g). Phytochemical screening In vitro analysis Quantification HPLC Gingerol Medicine R Homeopathy Tariq Mehmood verfasserin aut Saadia Zainab verfasserin aut Zulfiqar Ahmad verfasserin aut Aamir Shehzad verfasserin aut In Clinical Phytoscience SpringerOpen, 2015 6(2020), 1, Seite 12 (DE-627)835155773 (DE-600)2834057-7 21991197 nnns volume:6 year:2020 number:1 pages:12 https://doi.org/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/article/5a475f4514ca4ab4b05b4772f286feef kostenfrei http://link.springer.com/article/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/toc/2199-1197 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 1 12
spelling	10.1186/s40816-020-00158-z doi (DE-627)DOAJ056791208 (DE-599)DOAJ5a475f4514ca4ab4b05b4772f286feef DE-627 ger DE-627 rakwb eng RX1-681 Saira Tanweer verfasserin aut Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g). Phytochemical screening In vitro analysis Quantification HPLC Gingerol Medicine R Homeopathy Tariq Mehmood verfasserin aut Saadia Zainab verfasserin aut Zulfiqar Ahmad verfasserin aut Aamir Shehzad verfasserin aut In Clinical Phytoscience SpringerOpen, 2015 6(2020), 1, Seite 12 (DE-627)835155773 (DE-600)2834057-7 21991197 nnns volume:6 year:2020 number:1 pages:12 https://doi.org/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/article/5a475f4514ca4ab4b05b4772f286feef kostenfrei http://link.springer.com/article/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/toc/2199-1197 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 1 12
allfields_unstemmed	10.1186/s40816-020-00158-z doi (DE-627)DOAJ056791208 (DE-599)DOAJ5a475f4514ca4ab4b05b4772f286feef DE-627 ger DE-627 rakwb eng RX1-681 Saira Tanweer verfasserin aut Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g). Phytochemical screening In vitro analysis Quantification HPLC Gingerol Medicine R Homeopathy Tariq Mehmood verfasserin aut Saadia Zainab verfasserin aut Zulfiqar Ahmad verfasserin aut Aamir Shehzad verfasserin aut In Clinical Phytoscience SpringerOpen, 2015 6(2020), 1, Seite 12 (DE-627)835155773 (DE-600)2834057-7 21991197 nnns volume:6 year:2020 number:1 pages:12 https://doi.org/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/article/5a475f4514ca4ab4b05b4772f286feef kostenfrei http://link.springer.com/article/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/toc/2199-1197 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 1 12
allfieldsGer	10.1186/s40816-020-00158-z doi (DE-627)DOAJ056791208 (DE-599)DOAJ5a475f4514ca4ab4b05b4772f286feef DE-627 ger DE-627 rakwb eng RX1-681 Saira Tanweer verfasserin aut Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g). Phytochemical screening In vitro analysis Quantification HPLC Gingerol Medicine R Homeopathy Tariq Mehmood verfasserin aut Saadia Zainab verfasserin aut Zulfiqar Ahmad verfasserin aut Aamir Shehzad verfasserin aut In Clinical Phytoscience SpringerOpen, 2015 6(2020), 1, Seite 12 (DE-627)835155773 (DE-600)2834057-7 21991197 nnns volume:6 year:2020 number:1 pages:12 https://doi.org/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/article/5a475f4514ca4ab4b05b4772f286feef kostenfrei http://link.springer.com/article/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/toc/2199-1197 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 1 12
allfieldsSound	10.1186/s40816-020-00158-z doi (DE-627)DOAJ056791208 (DE-599)DOAJ5a475f4514ca4ab4b05b4772f286feef DE-627 ger DE-627 rakwb eng RX1-681 Saira Tanweer verfasserin aut Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g). Phytochemical screening In vitro analysis Quantification HPLC Gingerol Medicine R Homeopathy Tariq Mehmood verfasserin aut Saadia Zainab verfasserin aut Zulfiqar Ahmad verfasserin aut Aamir Shehzad verfasserin aut In Clinical Phytoscience SpringerOpen, 2015 6(2020), 1, Seite 12 (DE-627)835155773 (DE-600)2834057-7 21991197 nnns volume:6 year:2020 number:1 pages:12 https://doi.org/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/article/5a475f4514ca4ab4b05b4772f286feef kostenfrei http://link.springer.com/article/10.1186/s40816-020-00158-z kostenfrei https://doaj.org/toc/2199-1197 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 1 12
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Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g).</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Phytochemical screening</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">In vitro analysis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Quantification</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">HPLC</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gingerol</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Homeopathy</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Tariq 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callnumber-first	R - Medicine
author	Saira Tanweer
spellingShingle	Saira Tanweer misc RX1-681 misc Phytochemical screening misc In vitro analysis misc Quantification misc HPLC misc Gingerol misc Medicine misc R misc Homeopathy Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts
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topic_title	RX1-681 Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts Phytochemical screening In vitro analysis Quantification HPLC Gingerol
topic	misc RX1-681 misc Phytochemical screening misc In vitro analysis misc Quantification misc HPLC misc Gingerol misc Medicine misc R misc Homeopathy
topic_unstemmed	misc RX1-681 misc Phytochemical screening misc In vitro analysis misc Quantification misc HPLC misc Gingerol misc Medicine misc R misc Homeopathy
topic_browse	misc RX1-681 misc Phytochemical screening misc In vitro analysis misc Quantification misc HPLC misc Gingerol misc Medicine misc R misc Homeopathy
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title	Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts
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title_full	Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts
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author_browse	Saira Tanweer Tariq Mehmood Saadia Zainab Zulfiqar Ahmad Aamir Shehzad
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doi_str_mv	10.1186/s40816-020-00158-z
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title_sort	comparison and hplc quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts
callnumber	RX1-681
title_auth	Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts
abstract	Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g).
abstractGer	Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g).
abstract_unstemmed	Abstract Background In the present era, the attention of nutritionist diverted towards the bioactive entities present in natural sources owing to the presence of health boosting perspectives against lifestyle related disarrays. Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g).
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title_short	Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts
url	https://doi.org/10.1186/s40816-020-00158-z https://doaj.org/article/5a475f4514ca4ab4b05b4772f286feef http://link.springer.com/article/10.1186/s40816-020-00158-z https://doaj.org/toc/2199-1197
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author2	Tariq Mehmood Saadia Zainab Zulfiqar Ahmad Aamir Shehzad
author2Str	Tariq Mehmood Saadia Zainab Zulfiqar Ahmad Aamir Shehzad
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doi_str	10.1186/s40816-020-00158-z
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up_date	2024-07-03T22:52:01.044Z
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Methods In this context, different parts of ginger crop i.e. rhizome, leaves and flower of variety Suravi (ID no. 008) were used for the preparation of ginger extracts with 50% methanol, 50% ethanol and water via rotatory shaker for 45 min. After that, different phytochemical analysis and in vitro analyses were carried out to determine the antioxidant potential of these extracts. Lastly, the best selected extracts from each part was quantified through HPLC. Results The results of current investigated indicated that ethanol extract proved to have maximum quantity of phytoceutics as compared to methanol and water. The maximum TPC, flavonoids, flavonols, DPPH assay, antioxidant activity, FRAP assay, ABTS assay and metal chelating potential was observed in ginger leaves as 780.56 ± 32.78 GAE/100 g, 253.56 ± 10.65 mg/100 g, 49.54 ± 1.74 mg/100 g, 75.54 ± 3.17%, 77.88 ± 3.27%, 105.72 ± 4.44 μmole TE/g, 118.43 ± 4.97 μmole TE/g and 35.16 ± 1.48%, respectively followed by ginger flowers and ginger rhizome. The lowest antioxidant activity was estimated in ginger rhizome. On the basis of phytochemical profiling and in vitro analyses, ethanol extracts of ginger flowers, leaves and rhizome were selected for the quantification through HPLC. Conclusion The findings proved that maximum 6-gingerol was present in ginger leaves (4.9 mg/g) tackled by ginger flowers (2.87 mg/g) and ginger rhizome (1.03 mg/g).</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Phytochemical screening</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">In vitro analysis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Quantification</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">HPLC</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gingerol</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">R</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Homeopathy</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Tariq 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Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts

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