Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano

Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulatin...
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Gespeichert in:

Autor*in:	Michael Weber [verfasserIn] Athina Giannakara [verfasserIn] Steven A. Ramm [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence

Übergeordnetes Werk:	In: Ecology and Evolution - Wiley, 2012, 9(2019), 24, Seite 13889-13901
Übergeordnetes Werk:	volume:9 ; year:2019 ; number:24 ; pages:13889-13901

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1002/ece3.5825

Katalog-ID:	DOAJ056956029

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520			\|a Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid.
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700	0		\|a Steven A. Ramm \|e verfasserin \|4 aut
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author_variant	m w mw a g ag s a r sar
matchkey_str	article:20457758:2019----::eiafudeitdinsefcsnhsmlaeulhrahoiif
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publishDate	2019
allfields	10.1002/ece3.5825 doi (DE-627)DOAJ056956029 (DE-599)DOAJd21f7ff4f1104430aeb5f0d7cf28f54b DE-627 ger DE-627 rakwb eng QH540-549.5 Michael Weber verfasserin aut Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid. male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence Ecology Athina Giannakara verfasserin aut Steven A. Ramm verfasserin aut In Ecology and Evolution Wiley, 2012 9(2019), 24, Seite 13889-13901 (DE-627)671802984 (DE-600)2635675-2 20457758 nnns volume:9 year:2019 number:24 pages:13889-13901 https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/article/d21f7ff4f1104430aeb5f0d7cf28f54b kostenfrei https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/toc/2045-7758 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 24 13889-13901
spelling	10.1002/ece3.5825 doi (DE-627)DOAJ056956029 (DE-599)DOAJd21f7ff4f1104430aeb5f0d7cf28f54b DE-627 ger DE-627 rakwb eng QH540-549.5 Michael Weber verfasserin aut Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid. male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence Ecology Athina Giannakara verfasserin aut Steven A. Ramm verfasserin aut In Ecology and Evolution Wiley, 2012 9(2019), 24, Seite 13889-13901 (DE-627)671802984 (DE-600)2635675-2 20457758 nnns volume:9 year:2019 number:24 pages:13889-13901 https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/article/d21f7ff4f1104430aeb5f0d7cf28f54b kostenfrei https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/toc/2045-7758 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 24 13889-13901
allfields_unstemmed	10.1002/ece3.5825 doi (DE-627)DOAJ056956029 (DE-599)DOAJd21f7ff4f1104430aeb5f0d7cf28f54b DE-627 ger DE-627 rakwb eng QH540-549.5 Michael Weber verfasserin aut Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid. male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence Ecology Athina Giannakara verfasserin aut Steven A. Ramm verfasserin aut In Ecology and Evolution Wiley, 2012 9(2019), 24, Seite 13889-13901 (DE-627)671802984 (DE-600)2635675-2 20457758 nnns volume:9 year:2019 number:24 pages:13889-13901 https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/article/d21f7ff4f1104430aeb5f0d7cf28f54b kostenfrei https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/toc/2045-7758 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 24 13889-13901
allfieldsGer	10.1002/ece3.5825 doi (DE-627)DOAJ056956029 (DE-599)DOAJd21f7ff4f1104430aeb5f0d7cf28f54b DE-627 ger DE-627 rakwb eng QH540-549.5 Michael Weber verfasserin aut Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid. male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence Ecology Athina Giannakara verfasserin aut Steven A. Ramm verfasserin aut In Ecology and Evolution Wiley, 2012 9(2019), 24, Seite 13889-13901 (DE-627)671802984 (DE-600)2635675-2 20457758 nnns volume:9 year:2019 number:24 pages:13889-13901 https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/article/d21f7ff4f1104430aeb5f0d7cf28f54b kostenfrei https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/toc/2045-7758 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 24 13889-13901
allfieldsSound	10.1002/ece3.5825 doi (DE-627)DOAJ056956029 (DE-599)DOAJd21f7ff4f1104430aeb5f0d7cf28f54b DE-627 ger DE-627 rakwb eng QH540-549.5 Michael Weber verfasserin aut Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid. male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence Ecology Athina Giannakara verfasserin aut Steven A. Ramm verfasserin aut In Ecology and Evolution Wiley, 2012 9(2019), 24, Seite 13889-13901 (DE-627)671802984 (DE-600)2635675-2 20457758 nnns volume:9 year:2019 number:24 pages:13889-13901 https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/article/d21f7ff4f1104430aeb5f0d7cf28f54b kostenfrei https://doi.org/10.1002/ece3.5825 kostenfrei https://doaj.org/toc/2045-7758 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2019 24 13889-13901
language	English
source	In Ecology and Evolution 9(2019), 24, Seite 13889-13901 volume:9 year:2019 number:24 pages:13889-13901
sourceStr	In Ecology and Evolution 9(2019), 24, Seite 13889-13901 volume:9 year:2019 number:24 pages:13889-13901
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence Ecology
isfreeaccess_bool	true
container_title	Ecology and Evolution
authorswithroles_txt_mv	Michael Weber @@aut@@ Athina Giannakara @@aut@@ Steven A. Ramm @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	671802984
id	DOAJ056956029
language_de	englisch
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callnumber-first	Q - Science
author	Michael Weber
spellingShingle	Michael Weber misc QH540-549.5 misc male–male competition misc multiple mating misc seminal fluid misc sexual selection misc sperm competition misc sperm precedence misc Ecology Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano
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topic_title	QH540-549.5 Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano male–male competition multiple mating seminal fluid sexual selection sperm competition sperm precedence
topic	misc QH540-549.5 misc male–male competition misc multiple mating misc seminal fluid misc sexual selection misc sperm competition misc sperm precedence misc Ecology
topic_unstemmed	misc QH540-549.5 misc male–male competition misc multiple mating misc seminal fluid misc sexual selection misc sperm competition misc sperm precedence misc Ecology
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title	Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano
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title_full	Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano
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author_browse	Michael Weber Athina Giannakara Steven A. Ramm
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title_sort	seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm macrostomum lignano
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title_auth	Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano
abstract	Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid.
abstractGer	Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid.
abstract_unstemmed	Abstract As a class, seminal fluid proteins are expected to exert strong effects on mating partners due to the selection pressures of sperm competition and sexual conflict. But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid.
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container_issue	24
title_short	Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano
url	https://doi.org/10.1002/ece3.5825 https://doaj.org/article/d21f7ff4f1104430aeb5f0d7cf28f54b https://doaj.org/toc/2045-7758
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up_date	2024-07-03T23:41:59.938Z
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But because of the complexity of this secretion, linking specific proteins to downstream effects on own fitness—via manipulating the reproductive behavior, physiology, and ultimately the sperm utilization of mating partners—is not straightforward. Here, we adopted a systematic gene knockdown approach to screen for seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano. We focused on 18 transcripts in M. lignano seminal fluid, testing how their RNA interference‐induced knockdown impacted on three aspects of donor (male) reproductive success: (a) fertility (offspring production of the partner); (b) defensive sperm competitive ability, P1; and (c) offensive sperm competitive ability, P2. In general, the knockdown of most individual transcripts appeared to have only a minor impact on male reproductive success, though we found evidence that the knockdown of up to five different transcripts impacted on fertility; the knockdown of two other transcripts resulted in reduced P2; and knockdown of a further transcript actually increased P2. We thus identify a number of candidate seminal fluid transcripts that appear to modulate offspring production and sperm competitiveness in M. lignano. That only a minority of transcripts exhibit such a pattern likely reflects both the difficulty of accurately estimating sperm competitiveness and the functional redundancy of seminal fluid.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">male–male competition</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">multiple mating</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">seminal fluid</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">sexual selection</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">sperm competition</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">sperm precedence</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Ecology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Athina Giannakara</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Steven A. 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Seminal fluid‐mediated fitness effects in the simultaneously hermaphroditic flatworm Macrostomum lignano

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