Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<

<i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovere...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Fabiana Nitz [verfasserIn] Bruna Oliveira de Melo [verfasserIn] Luís Cláudio Nascimento da Silva [verfasserIn] Andrea de Souza Monteiro [verfasserIn] Sirlei Garcia Marques [verfasserIn] Valério Monteiro-Neto [verfasserIn] Rosimary de Jesus Gomes Turri [verfasserIn] Antonio Dantas Silva Junior [verfasserIn] Patrícia Cristina Ribeiro Conceição [verfasserIn] Hilário José Cardoso Magalhães [verfasserIn] Adrielle Zagmignan [verfasserIn] Thiago Azevedo Feitosa Ferro [verfasserIn] Maria Rosa Quaresma Bomfim [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	extensive drug resistance (XDR) oxacillinase genes

Übergeordnetes Werk:	In: Microorganisms - MDPI AG, 2013, 9(2021), 4, p 786
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:4, p 786

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/microorganisms9040786

Katalog-ID:	DOAJ057075239

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ057075239
003	DE-627
005	20240412184556.0
007	cr uuu---uuuuu
008	230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.3390/microorganisms9040786 \|2 doi
035			\|a (DE-627)DOAJ057075239
035			\|a (DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a QH301-705.5
100	0		\|a Fabiana Nitz \|e verfasserin \|4 aut
245	1	0	\|a Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<
264		1	\|c 2021
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a <i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<.
650		4	\|a extensive drug resistance (XDR)
650		4	\|a oxacillinase genes
650		4	\|a <i<Pseudomonas aeruginosa</i<
650		4	\|a <i<mcr-1</i< gene
653		0	\|a Biology (General)
700	0		\|a Bruna Oliveira de Melo \|e verfasserin \|4 aut
700	0		\|a Luís Cláudio Nascimento da Silva \|e verfasserin \|4 aut
700	0		\|a Andrea de Souza Monteiro \|e verfasserin \|4 aut
700	0		\|a Sirlei Garcia Marques \|e verfasserin \|4 aut
700	0		\|a Valério Monteiro-Neto \|e verfasserin \|4 aut
700	0		\|a Rosimary de Jesus Gomes Turri \|e verfasserin \|4 aut
700	0		\|a Antonio Dantas Silva Junior \|e verfasserin \|4 aut
700	0		\|a Patrícia Cristina Ribeiro Conceição \|e verfasserin \|4 aut
700	0		\|a Hilário José Cardoso Magalhães \|e verfasserin \|4 aut
700	0		\|a Adrielle Zagmignan \|e verfasserin \|4 aut
700	0		\|a Thiago Azevedo Feitosa Ferro \|e verfasserin \|4 aut
700	0		\|a Maria Rosa Quaresma Bomfim \|e verfasserin \|4 aut
773	0	8	\|i In \|t Microorganisms \|d MDPI AG, 2013 \|g 9(2021), 4, p 786 \|w (DE-627)750370696 \|w (DE-600)2720891-6 \|x 20762607 \|7 nnns
773	1	8	\|g volume:9 \|g year:2021 \|g number:4, p 786
856	4	0	\|u https://doi.org/10.3390/microorganisms9040786 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9 \|z kostenfrei
856	4	0	\|u https://www.mdpi.com/2076-2607/9/4/786 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/2076-2607 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 9 \|j 2021 \|e 4, p 786

Indexfelder

author_variant	f n fn b o d m bodm l c n d s lcnds a d s m adsm s g m sgm v m n vmn r d j g t rdjgt a d s j adsj p c r c pcrc h j c m hjcm a z az t a f f taff m r q b mrqb
matchkey_str	article:20762607:2021----::oeuadtcinfrgeitneeeoiliuoa3uiliuoa1uadmrinlncl
hierarchy_sort_str	2021
callnumber-subject-code	QH
publishDate	2021
allfields	10.3390/microorganisms9040786 doi (DE-627)DOAJ057075239 (DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9 DE-627 ger DE-627 rakwb eng QH301-705.5 Fabiana Nitz verfasserin aut Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<. extensive drug resistance (XDR) oxacillinase genes <i<Pseudomonas aeruginosa</i< <i<mcr-1</i< gene Biology (General) Bruna Oliveira de Melo verfasserin aut Luís Cláudio Nascimento da Silva verfasserin aut Andrea de Souza Monteiro verfasserin aut Sirlei Garcia Marques verfasserin aut Valério Monteiro-Neto verfasserin aut Rosimary de Jesus Gomes Turri verfasserin aut Antonio Dantas Silva Junior verfasserin aut Patrícia Cristina Ribeiro Conceição verfasserin aut Hilário José Cardoso Magalhães verfasserin aut Adrielle Zagmignan verfasserin aut Thiago Azevedo Feitosa Ferro verfasserin aut Maria Rosa Quaresma Bomfim verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 4, p 786 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:4, p 786 https://doi.org/10.3390/microorganisms9040786 kostenfrei https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9 kostenfrei https://www.mdpi.com/2076-2607/9/4/786 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4, p 786
spelling	10.3390/microorganisms9040786 doi (DE-627)DOAJ057075239 (DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9 DE-627 ger DE-627 rakwb eng QH301-705.5 Fabiana Nitz verfasserin aut Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<. extensive drug resistance (XDR) oxacillinase genes <i<Pseudomonas aeruginosa</i< <i<mcr-1</i< gene Biology (General) Bruna Oliveira de Melo verfasserin aut Luís Cláudio Nascimento da Silva verfasserin aut Andrea de Souza Monteiro verfasserin aut Sirlei Garcia Marques verfasserin aut Valério Monteiro-Neto verfasserin aut Rosimary de Jesus Gomes Turri verfasserin aut Antonio Dantas Silva Junior verfasserin aut Patrícia Cristina Ribeiro Conceição verfasserin aut Hilário José Cardoso Magalhães verfasserin aut Adrielle Zagmignan verfasserin aut Thiago Azevedo Feitosa Ferro verfasserin aut Maria Rosa Quaresma Bomfim verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 4, p 786 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:4, p 786 https://doi.org/10.3390/microorganisms9040786 kostenfrei https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9 kostenfrei https://www.mdpi.com/2076-2607/9/4/786 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4, p 786
allfields_unstemmed	10.3390/microorganisms9040786 doi (DE-627)DOAJ057075239 (DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9 DE-627 ger DE-627 rakwb eng QH301-705.5 Fabiana Nitz verfasserin aut Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<. extensive drug resistance (XDR) oxacillinase genes <i<Pseudomonas aeruginosa</i< <i<mcr-1</i< gene Biology (General) Bruna Oliveira de Melo verfasserin aut Luís Cláudio Nascimento da Silva verfasserin aut Andrea de Souza Monteiro verfasserin aut Sirlei Garcia Marques verfasserin aut Valério Monteiro-Neto verfasserin aut Rosimary de Jesus Gomes Turri verfasserin aut Antonio Dantas Silva Junior verfasserin aut Patrícia Cristina Ribeiro Conceição verfasserin aut Hilário José Cardoso Magalhães verfasserin aut Adrielle Zagmignan verfasserin aut Thiago Azevedo Feitosa Ferro verfasserin aut Maria Rosa Quaresma Bomfim verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 4, p 786 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:4, p 786 https://doi.org/10.3390/microorganisms9040786 kostenfrei https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9 kostenfrei https://www.mdpi.com/2076-2607/9/4/786 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4, p 786
allfieldsGer	10.3390/microorganisms9040786 doi (DE-627)DOAJ057075239 (DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9 DE-627 ger DE-627 rakwb eng QH301-705.5 Fabiana Nitz verfasserin aut Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<. extensive drug resistance (XDR) oxacillinase genes <i<Pseudomonas aeruginosa</i< <i<mcr-1</i< gene Biology (General) Bruna Oliveira de Melo verfasserin aut Luís Cláudio Nascimento da Silva verfasserin aut Andrea de Souza Monteiro verfasserin aut Sirlei Garcia Marques verfasserin aut Valério Monteiro-Neto verfasserin aut Rosimary de Jesus Gomes Turri verfasserin aut Antonio Dantas Silva Junior verfasserin aut Patrícia Cristina Ribeiro Conceição verfasserin aut Hilário José Cardoso Magalhães verfasserin aut Adrielle Zagmignan verfasserin aut Thiago Azevedo Feitosa Ferro verfasserin aut Maria Rosa Quaresma Bomfim verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 4, p 786 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:4, p 786 https://doi.org/10.3390/microorganisms9040786 kostenfrei https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9 kostenfrei https://www.mdpi.com/2076-2607/9/4/786 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4, p 786
allfieldsSound	10.3390/microorganisms9040786 doi (DE-627)DOAJ057075239 (DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9 DE-627 ger DE-627 rakwb eng QH301-705.5 Fabiana Nitz verfasserin aut Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<. extensive drug resistance (XDR) oxacillinase genes <i<Pseudomonas aeruginosa</i< <i<mcr-1</i< gene Biology (General) Bruna Oliveira de Melo verfasserin aut Luís Cláudio Nascimento da Silva verfasserin aut Andrea de Souza Monteiro verfasserin aut Sirlei Garcia Marques verfasserin aut Valério Monteiro-Neto verfasserin aut Rosimary de Jesus Gomes Turri verfasserin aut Antonio Dantas Silva Junior verfasserin aut Patrícia Cristina Ribeiro Conceição verfasserin aut Hilário José Cardoso Magalhães verfasserin aut Adrielle Zagmignan verfasserin aut Thiago Azevedo Feitosa Ferro verfasserin aut Maria Rosa Quaresma Bomfim verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 4, p 786 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:4, p 786 https://doi.org/10.3390/microorganisms9040786 kostenfrei https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9 kostenfrei https://www.mdpi.com/2076-2607/9/4/786 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 4, p 786
language	English
source	In Microorganisms 9(2021), 4, p 786 volume:9 year:2021 number:4, p 786
sourceStr	In Microorganisms 9(2021), 4, p 786 volume:9 year:2021 number:4, p 786
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	extensive drug resistance (XDR) oxacillinase genes <i<Pseudomonas aeruginosa</i< <i<mcr-1</i< gene Biology (General)
isfreeaccess_bool	true
container_title	Microorganisms
authorswithroles_txt_mv	Fabiana Nitz @@aut@@ Bruna Oliveira de Melo @@aut@@ Luís Cláudio Nascimento da Silva @@aut@@ Andrea de Souza Monteiro @@aut@@ Sirlei Garcia Marques @@aut@@ Valério Monteiro-Neto @@aut@@ Rosimary de Jesus Gomes Turri @@aut@@ Antonio Dantas Silva Junior @@aut@@ Patrícia Cristina Ribeiro Conceição @@aut@@ Hilário José Cardoso Magalhães @@aut@@ Adrielle Zagmignan @@aut@@ Thiago Azevedo Feitosa Ferro @@aut@@ Maria Rosa Quaresma Bomfim @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	750370696
id	DOAJ057075239
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ057075239</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412184556.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/microorganisms9040786</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ057075239</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Fabiana Nitz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">extensive drug resistance (XDR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">oxacillinase genes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Pseudomonas aeruginosa</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<mcr-1</i< gene</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Bruna Oliveira de Melo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Luís Cláudio Nascimento da Silva</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Andrea de Souza Monteiro</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sirlei Garcia Marques</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Valério Monteiro-Neto</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Rosimary de Jesus Gomes Turri</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Antonio Dantas Silva Junior</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Patrícia Cristina Ribeiro Conceição</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hilário José Cardoso Magalhães</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Adrielle Zagmignan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Thiago Azevedo Feitosa Ferro</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Maria Rosa Quaresma Bomfim</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Microorganisms</subfield><subfield code="d">MDPI AG, 2013</subfield><subfield code="g">9(2021), 4, p 786</subfield><subfield code="w">(DE-627)750370696</subfield><subfield code="w">(DE-600)2720891-6</subfield><subfield code="x">20762607</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:9</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:4, p 786</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/microorganisms9040786</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2076-2607/9/4/786</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2076-2607</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">9</subfield><subfield code="j">2021</subfield><subfield code="e">4, p 786</subfield></datafield></record></collection>
callnumber-first	Q - Science
author	Fabiana Nitz
spellingShingle	Fabiana Nitz misc QH301-705.5 misc extensive drug resistance (XDR) misc oxacillinase genes misc <i<Pseudomonas aeruginosa</i< misc <i<mcr-1</i< gene misc Biology (General) Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<
authorStr	Fabiana Nitz
ppnlink_with_tag_str_mv	@@773@@(DE-627)750370696
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	QH301-705
illustrated	Not Illustrated
issn	20762607
topic_title	QH301-705.5 Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i< extensive drug resistance (XDR) oxacillinase genes <i<Pseudomonas aeruginosa</i< <i<mcr-1</i< gene
topic	misc QH301-705.5 misc extensive drug resistance (XDR) misc oxacillinase genes misc <i<Pseudomonas aeruginosa</i< misc <i<mcr-1</i< gene misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc extensive drug resistance (XDR) misc oxacillinase genes misc <i<Pseudomonas aeruginosa</i< misc <i<mcr-1</i< gene misc Biology (General)
topic_browse	misc QH301-705.5 misc extensive drug resistance (XDR) misc oxacillinase genes misc <i<Pseudomonas aeruginosa</i< misc <i<mcr-1</i< gene misc Biology (General)
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Microorganisms
hierarchy_parent_id	750370696
hierarchy_top_title	Microorganisms
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)750370696 (DE-600)2720891-6
title	Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<
ctrlnum	(DE-627)DOAJ057075239 (DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9
title_full	Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<
author_sort	Fabiana Nitz
journal	Microorganisms
journalStr	Microorganisms
callnumber-first-code	Q
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2021
contenttype_str_mv	txt
author_browse	Fabiana Nitz Bruna Oliveira de Melo Luís Cláudio Nascimento da Silva Andrea de Souza Monteiro Sirlei Garcia Marques Valério Monteiro-Neto Rosimary de Jesus Gomes Turri Antonio Dantas Silva Junior Patrícia Cristina Ribeiro Conceição Hilário José Cardoso Magalhães Adrielle Zagmignan Thiago Azevedo Feitosa Ferro Maria Rosa Quaresma Bomfim
container_volume	9
class	QH301-705.5
format_se	Elektronische Aufsätze
author-letter	Fabiana Nitz
doi_str_mv	10.3390/microorganisms9040786
author2-role	verfasserin
title_sort	molecular detection of drug-resistance genes of <i<bla</i<<sub<oxa-23</sub<<i<-bla</i<<sub<oxa-51</sub< and <i<mcr-1</i< in clinical isolates of <i<pseudomonas aeruginosa</i<
callnumber	QH301-705.5
title_auth	Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<
abstract	<i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<.
abstractGer	<i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<.
abstract_unstemmed	<i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	4, p 786
title_short	Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<
url	https://doi.org/10.3390/microorganisms9040786 https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9 https://www.mdpi.com/2076-2607/9/4/786 https://doaj.org/toc/2076-2607
remote_bool	true
author2	Bruna Oliveira de Melo Luís Cláudio Nascimento da Silva Andrea de Souza Monteiro Sirlei Garcia Marques Valério Monteiro-Neto Rosimary de Jesus Gomes Turri Antonio Dantas Silva Junior Patrícia Cristina Ribeiro Conceição Hilário José Cardoso Magalhães Adrielle Zagmignan Thiago Azevedo Feitosa Ferro Maria Rosa Quaresma Bomfim
author2Str	Bruna Oliveira de Melo Luís Cláudio Nascimento da Silva Andrea de Souza Monteiro Sirlei Garcia Marques Valério Monteiro-Neto Rosimary de Jesus Gomes Turri Antonio Dantas Silva Junior Patrícia Cristina Ribeiro Conceição Hilário José Cardoso Magalhães Adrielle Zagmignan Thiago Azevedo Feitosa Ferro Maria Rosa Quaresma Bomfim
ppnlink	750370696
callnumber-subject	QH - Natural History and Biology
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.3390/microorganisms9040786
callnumber-a	QH301-705.5
up_date	2024-07-04T00:12:59.034Z
_version_	1803605223970701312
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ057075239</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412184556.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/microorganisms9040786</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ057075239</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJed8943e1ad3f443fb5bf495d737c51b9</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Fabiana Nitz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><i<Pseudomonas aeruginosa</i< has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of <i<P. aeruginosa</i< recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among <i<P. aeruginosa</i< isolates. The drug susceptibility profiles of <i<P. aeruginosa</i< for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (<i<mcr-1</i<) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (<i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub<) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of <i<bla</i<<sub<OXA-23</sub< and <i<bla</i<<sub<OXA-51</sub< genes in <i<P. aeruginosa</i<.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">extensive drug resistance (XDR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">oxacillinase genes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Pseudomonas aeruginosa</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<mcr-1</i< gene</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Bruna Oliveira de Melo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Luís Cláudio Nascimento da Silva</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Andrea de Souza Monteiro</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Sirlei Garcia Marques</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Valério Monteiro-Neto</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Rosimary de Jesus Gomes Turri</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Antonio Dantas Silva Junior</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Patrícia Cristina Ribeiro Conceição</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hilário José Cardoso Magalhães</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Adrielle Zagmignan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Thiago Azevedo Feitosa Ferro</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Maria Rosa Quaresma Bomfim</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Microorganisms</subfield><subfield code="d">MDPI AG, 2013</subfield><subfield code="g">9(2021), 4, p 786</subfield><subfield code="w">(DE-627)750370696</subfield><subfield code="w">(DE-600)2720891-6</subfield><subfield code="x">20762607</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:9</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:4, p 786</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/microorganisms9040786</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/ed8943e1ad3f443fb5bf495d737c51b9</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/2076-2607/9/4/786</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2076-2607</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">9</subfield><subfield code="j">2021</subfield><subfield code="e">4, p 786</subfield></datafield></record></collection>
score	7.401967

Nicht das Richtige dabei?

Schreiben Sie uns!

Molecular Detection of Drug-Resistance Genes of <i<bla</i<<sub<OXA-23</sub<<i<-bla</i<<sub<OXA-51</sub< and <i<mcr-1</i< in Clinical Isolates of <i<Pseudomonas aeruginosa</i<

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?