Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase

Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying a...
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Autor*in:	Yiou Pan [verfasserIn] Xiaochun Zeng [verfasserIn] Shuyuan Wen [verfasserIn] Xuemei Liu [verfasserIn] Qingli Shang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	CNC-bZIP superoxide dismutase transcription regulation RNAi

Übergeordnetes Werk:	In: Insects - MDPI AG, 2011, 11(2020), 4, p 221
Übergeordnetes Werk:	volume:11 ; year:2020 ; number:4, p 221

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/insects11040221

Katalog-ID:	DOAJ057250820

Internformat


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allfields	10.3390/insects11040221 doi (DE-627)DOAJ057250820 (DE-599)DOAJ7d312cc3a7b14e9881022f3d344418f4 DE-627 ger DE-627 rakwb eng Yiou Pan verfasserin aut Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i< CNC-bZIP superoxide dismutase transcription regulation RNAi <i<Spodoptera frugiperda</i< Science Q Xiaochun Zeng verfasserin aut Shuyuan Wen verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In Insects MDPI AG, 2011 11(2020), 4, p 221 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:11 year:2020 number:4, p 221 https://doi.org/10.3390/insects11040221 kostenfrei https://doaj.org/article/7d312cc3a7b14e9881022f3d344418f4 kostenfrei https://www.mdpi.com/2075-4450/11/4/221 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 221
spelling	10.3390/insects11040221 doi (DE-627)DOAJ057250820 (DE-599)DOAJ7d312cc3a7b14e9881022f3d344418f4 DE-627 ger DE-627 rakwb eng Yiou Pan verfasserin aut Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i< CNC-bZIP superoxide dismutase transcription regulation RNAi <i<Spodoptera frugiperda</i< Science Q Xiaochun Zeng verfasserin aut Shuyuan Wen verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In Insects MDPI AG, 2011 11(2020), 4, p 221 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:11 year:2020 number:4, p 221 https://doi.org/10.3390/insects11040221 kostenfrei https://doaj.org/article/7d312cc3a7b14e9881022f3d344418f4 kostenfrei https://www.mdpi.com/2075-4450/11/4/221 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 221
allfields_unstemmed	10.3390/insects11040221 doi (DE-627)DOAJ057250820 (DE-599)DOAJ7d312cc3a7b14e9881022f3d344418f4 DE-627 ger DE-627 rakwb eng Yiou Pan verfasserin aut Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i< CNC-bZIP superoxide dismutase transcription regulation RNAi <i<Spodoptera frugiperda</i< Science Q Xiaochun Zeng verfasserin aut Shuyuan Wen verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In Insects MDPI AG, 2011 11(2020), 4, p 221 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:11 year:2020 number:4, p 221 https://doi.org/10.3390/insects11040221 kostenfrei https://doaj.org/article/7d312cc3a7b14e9881022f3d344418f4 kostenfrei https://www.mdpi.com/2075-4450/11/4/221 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 221
allfieldsGer	10.3390/insects11040221 doi (DE-627)DOAJ057250820 (DE-599)DOAJ7d312cc3a7b14e9881022f3d344418f4 DE-627 ger DE-627 rakwb eng Yiou Pan verfasserin aut Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i< CNC-bZIP superoxide dismutase transcription regulation RNAi <i<Spodoptera frugiperda</i< Science Q Xiaochun Zeng verfasserin aut Shuyuan Wen verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In Insects MDPI AG, 2011 11(2020), 4, p 221 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:11 year:2020 number:4, p 221 https://doi.org/10.3390/insects11040221 kostenfrei https://doaj.org/article/7d312cc3a7b14e9881022f3d344418f4 kostenfrei https://www.mdpi.com/2075-4450/11/4/221 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 221
allfieldsSound	10.3390/insects11040221 doi (DE-627)DOAJ057250820 (DE-599)DOAJ7d312cc3a7b14e9881022f3d344418f4 DE-627 ger DE-627 rakwb eng Yiou Pan verfasserin aut Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i< CNC-bZIP superoxide dismutase transcription regulation RNAi <i<Spodoptera frugiperda</i< Science Q Xiaochun Zeng verfasserin aut Shuyuan Wen verfasserin aut Xuemei Liu verfasserin aut Qingli Shang verfasserin aut In Insects MDPI AG, 2011 11(2020), 4, p 221 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:11 year:2020 number:4, p 221 https://doi.org/10.3390/insects11040221 kostenfrei https://doaj.org/article/7d312cc3a7b14e9881022f3d344418f4 kostenfrei https://www.mdpi.com/2075-4450/11/4/221 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 4, p 221
language	English
source	In Insects 11(2020), 4, p 221 volume:11 year:2020 number:4, p 221
sourceStr	In Insects 11(2020), 4, p 221 volume:11 year:2020 number:4, p 221
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CNC-bZIP superoxide dismutase transcription regulation RNAi <i<Spodoptera frugiperda</i< Science Q
isfreeaccess_bool	true
container_title	Insects
authorswithroles_txt_mv	Yiou Pan @@aut@@ Xiaochun Zeng @@aut@@ Shuyuan Wen @@aut@@ Xuemei Liu @@aut@@ Qingli Shang @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	718627121
id	DOAJ057250820
language_de	englisch
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author	Yiou Pan
spellingShingle	Yiou Pan misc CNC-bZIP misc superoxide dismutase misc transcription regulation misc RNAi misc <i<Spodoptera frugiperda</i< misc Science misc Q Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase
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topic_title	Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase CNC-bZIP superoxide dismutase transcription regulation RNAi <i<Spodoptera frugiperda</i<
topic	misc CNC-bZIP misc superoxide dismutase misc transcription regulation misc RNAi misc <i<Spodoptera frugiperda</i< misc Science misc Q
topic_unstemmed	misc CNC-bZIP misc superoxide dismutase misc transcription regulation misc RNAi misc <i<Spodoptera frugiperda</i< misc Science misc Q
topic_browse	misc CNC-bZIP misc superoxide dismutase misc transcription regulation misc RNAi misc <i<Spodoptera frugiperda</i< misc Science misc Q
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title	Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase
ctrlnum	(DE-627)DOAJ057250820 (DE-599)DOAJ7d312cc3a7b14e9881022f3d344418f4
title_full	Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase
author_sort	Yiou Pan
journal	Insects
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author_browse	Yiou Pan Xiaochun Zeng Shuyuan Wen Xuemei Liu Qingli Shang
container_volume	11
format_se	Elektronische Aufsätze
author-letter	Yiou Pan
doi_str_mv	10.3390/insects11040221
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title_sort	characterization of the cap ‘n’ collar isoform c gene in <i<spodoptera frugiperda</i< and its association with superoxide dismutase
title_auth	Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase
abstract	Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i<
abstractGer	Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i<
abstract_unstemmed	Nuclear factor erythroid 2 related factor 2 (Nrf2) belongs to the cap ‘n’ collar basic region leucine zipper (CNC-bZIP) transcription factor family, and is activated by diverse oxidants, pro-oxidants, antioxidants, and chemo-preventive agents. Transcriptional regulation of a battery of detoxifying and antioxidant genes by Nrf2 has been shown to be important for protection against oxidative stress or chemically-induced cellular damages. In our research, we cloned the full length CncC gene from the <i<Spodoptera frugiperda</i<, named as <i<Sf</i<CncC. The cDNA of the <i<Sf</i<CncC consists of 2652 nucleotides that include a 2196-nucleotide open reading frame (ORF), encoding 731 amino acid residues, and 239- and 217-bp non-coding regions flanking at the 5’- and 3’-ends of the cDNA, respectively. Sequence analysis indicated <i<Sf</i<CncC has the conserved domain (CNC-bZIP domain and a tetrapeptide motif, ETGE) character of Nrf2 and showed high identity compared with the CncC/Nrf2 from other insect and vertebrate species. Over-expression of <i<Sf</i<CncC can up-regulate the transcription and activity of the SOD gene in Sf9 cells, and the RNAi of <i<Sf</i<CncC in Sf9 cells and larvae of <i<S. frugiperda</i< can dramatically reduce the transcriptional level and activity of the SOD gene, as determined by real-time quantitative PCRs. So the <i<Sf</i<CncC is involved in the Keap1-Nrf2-ARE pathway, acting the same as the transcriptional factor Nrf2 in vertebrate, and plays a role for host cell defense. The functional characterization of <i<Sf</i<CncC provides the fundamental basis for us to further understand the regulatory mechanism of anti-oxidants and anti-xenobiotics in <i<S. frugiperda.</i<
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container_issue	4, p 221
title_short	Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase
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Characterization of the Cap ‘n’ Collar Isoform C gene in <i<Spodoptera frugiperda</i< and its Association with Superoxide Dismutase

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