Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation

As an abundant marine xanthophyll, fucoxanthin (FX) exhibits a broad range of biological activities. The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple an...
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Autor*in:	Yuemei Ye [verfasserIn] Jingwen Sun [verfasserIn] Liting Wang [verfasserIn] Junwang Zhu [verfasserIn] Wei Cui [verfasserIn] Hongyan Hou [verfasserIn] Jinrong Zhang [verfasserIn] Chengxu Zhou [verfasserIn] Xiaojun Yan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	fucoxanthin ODS column chromatography ethanol precipitation purification brown seaweed

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 26(2021), 13, p 3777
Übergeordnetes Werk:	volume:26 ; year:2021 ; number:13, p 3777

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules26133777

Katalog-ID:	DOAJ05743090X

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allfieldsSound	10.3390/molecules26133777 doi (DE-627)DOAJ05743090X (DE-599)DOAJb6a2c756c0fd4a06b75d0a0fbf411c82 DE-627 ger DE-627 rakwb eng QD241-441 Yuemei Ye verfasserin aut Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As an abundant marine xanthophyll, fucoxanthin (FX) exhibits a broad range of biological activities. The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple and efficient purification approach for the purification of FX from the brown seaweed <i<Sargassum horneri</i< was carried out. The FX-rich ethanol extract was isolated by octadecylsilyl (ODS) column chromatography using ethanol–water solvent as a gradient eluent. The overwhelming majority of FX was successfully eluted by the ethanol–water mixture (9:1, <i<v</i</<i<v</i<), with a recovery rate of 95.36%. A parametric study was performed to optimize the aqueous ethanol precipitation process by investigating the effects on the purity and recovery of FX. Under the optimal conditions, the purity of FX was 91.07%, and the recovery rate was 74.98%. Collectively, the eco-friendly method was cost-efficient for the purification of FX. The developed method provides a potential approach for the large-scale production of fucoxanthin from the brown seaweed <i<Sargassum horneri.</i< fucoxanthin ODS column chromatography ethanol precipitation purification brown seaweed Organic chemistry Jingwen Sun verfasserin aut Liting Wang verfasserin aut Junwang Zhu verfasserin aut Wei Cui verfasserin aut Hongyan Hou verfasserin aut Jinrong Zhang verfasserin aut Chengxu Zhou verfasserin aut Xiaojun Yan verfasserin aut In Molecules MDPI AG, 2003 26(2021), 13, p 3777 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:26 year:2021 number:13, p 3777 https://doi.org/10.3390/molecules26133777 kostenfrei https://doaj.org/article/b6a2c756c0fd4a06b75d0a0fbf411c82 kostenfrei https://www.mdpi.com/1420-3049/26/13/3777 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26 2021 13, p 3777
language	English
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callnumber-first	Q - Science
author	Yuemei Ye
spellingShingle	Yuemei Ye misc QD241-441 misc fucoxanthin misc ODS column chromatography misc ethanol precipitation misc purification misc brown seaweed misc Organic chemistry Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation
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topic_title	QD241-441 Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation fucoxanthin ODS column chromatography ethanol precipitation purification brown seaweed
topic	misc QD241-441 misc fucoxanthin misc ODS column chromatography misc ethanol precipitation misc purification misc brown seaweed misc Organic chemistry
topic_unstemmed	misc QD241-441 misc fucoxanthin misc ODS column chromatography misc ethanol precipitation misc purification misc brown seaweed misc Organic chemistry
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title	Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation
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title_full	Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation
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author_browse	Yuemei Ye Jingwen Sun Liting Wang Junwang Zhu Wei Cui Hongyan Hou Jinrong Zhang Chengxu Zhou Xiaojun Yan
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title_sort	isolation and purification of fucoxanthin from brown seaweed <i<sargassum horneri</i< using open ods column chromatography and ethanol precipitation
callnumber	QD241-441
title_auth	Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation
abstract	As an abundant marine xanthophyll, fucoxanthin (FX) exhibits a broad range of biological activities. The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple and efficient purification approach for the purification of FX from the brown seaweed <i<Sargassum horneri</i< was carried out. The FX-rich ethanol extract was isolated by octadecylsilyl (ODS) column chromatography using ethanol–water solvent as a gradient eluent. The overwhelming majority of FX was successfully eluted by the ethanol–water mixture (9:1, <i<v</i</<i<v</i<), with a recovery rate of 95.36%. A parametric study was performed to optimize the aqueous ethanol precipitation process by investigating the effects on the purity and recovery of FX. Under the optimal conditions, the purity of FX was 91.07%, and the recovery rate was 74.98%. Collectively, the eco-friendly method was cost-efficient for the purification of FX. The developed method provides a potential approach for the large-scale production of fucoxanthin from the brown seaweed <i<Sargassum horneri.</i<
abstractGer	As an abundant marine xanthophyll, fucoxanthin (FX) exhibits a broad range of biological activities. The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple and efficient purification approach for the purification of FX from the brown seaweed <i<Sargassum horneri</i< was carried out. The FX-rich ethanol extract was isolated by octadecylsilyl (ODS) column chromatography using ethanol–water solvent as a gradient eluent. The overwhelming majority of FX was successfully eluted by the ethanol–water mixture (9:1, <i<v</i</<i<v</i<), with a recovery rate of 95.36%. A parametric study was performed to optimize the aqueous ethanol precipitation process by investigating the effects on the purity and recovery of FX. Under the optimal conditions, the purity of FX was 91.07%, and the recovery rate was 74.98%. Collectively, the eco-friendly method was cost-efficient for the purification of FX. The developed method provides a potential approach for the large-scale production of fucoxanthin from the brown seaweed <i<Sargassum horneri.</i<
abstract_unstemmed	As an abundant marine xanthophyll, fucoxanthin (FX) exhibits a broad range of biological activities. The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple and efficient purification approach for the purification of FX from the brown seaweed <i<Sargassum horneri</i< was carried out. The FX-rich ethanol extract was isolated by octadecylsilyl (ODS) column chromatography using ethanol–water solvent as a gradient eluent. The overwhelming majority of FX was successfully eluted by the ethanol–water mixture (9:1, <i<v</i</<i<v</i<), with a recovery rate of 95.36%. A parametric study was performed to optimize the aqueous ethanol precipitation process by investigating the effects on the purity and recovery of FX. Under the optimal conditions, the purity of FX was 91.07%, and the recovery rate was 74.98%. Collectively, the eco-friendly method was cost-efficient for the purification of FX. The developed method provides a potential approach for the large-scale production of fucoxanthin from the brown seaweed <i<Sargassum horneri.</i<
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container_issue	13, p 3777
title_short	Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation
url	https://doi.org/10.3390/molecules26133777 https://doaj.org/article/b6a2c756c0fd4a06b75d0a0fbf411c82 https://www.mdpi.com/1420-3049/26/13/3777 https://doaj.org/toc/1420-3049
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author2	Jingwen Sun Liting Wang Junwang Zhu Wei Cui Hongyan Hou Jinrong Zhang Chengxu Zhou Xiaojun Yan
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up_date	2024-07-04T01:38:12.838Z
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The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple and efficient purification approach for the purification of FX from the brown seaweed <i<Sargassum horneri</i< was carried out. The FX-rich ethanol extract was isolated by octadecylsilyl (ODS) column chromatography using ethanol–water solvent as a gradient eluent. The overwhelming majority of FX was successfully eluted by the ethanol–water mixture (9:1, <i<v</i</<i<v</i<), with a recovery rate of 95.36%. A parametric study was performed to optimize the aqueous ethanol precipitation process by investigating the effects on the purity and recovery of FX. Under the optimal conditions, the purity of FX was 91.07%, and the recovery rate was 74.98%. Collectively, the eco-friendly method was cost-efficient for the purification of FX. 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Isolation and Purification of Fucoxanthin from Brown Seaweed <i<Sargassum horneri</i< Using Open ODS Column Chromatography and Ethanol Precipitation

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