Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings
Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (sy...
Ausführliche Beschreibung
Autor*in: |
Lumei Liu [verfasserIn] Sayali Dharmadhikari [verfasserIn] Robert A. Pouliot [verfasserIn] Michael M. Li [verfasserIn] Peter M. Minneci [verfasserIn] Zhenghong Tan [verfasserIn] Kimberly Shontz [verfasserIn] Jed Johnson [verfasserIn] Susan D. Reynolds [verfasserIn] Christopher K. Breuer [verfasserIn] Daniel J. Weiss [verfasserIn] Tendy Chiang [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2021 |
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Schlagwörter: |
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Übergeordnetes Werk: |
In: Bioengineering - MDPI AG, 2014, 8(2021), 8, p 116 |
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Übergeordnetes Werk: |
volume:8 ; year:2021 ; number:8, p 116 |
Links: |
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DOI / URN: |
10.3390/bioengineering8080116 |
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Katalog-ID: |
DOAJ057556873 |
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10.3390/bioengineering8080116 doi (DE-627)DOAJ057556873 (DE-599)DOAJeb93d533b79e4011a9d32ee721dd0c94 DE-627 ger DE-627 rakwb eng QH301-705.5 Lumei Liu verfasserin aut Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning Technology T Biology (General) Sayali Dharmadhikari verfasserin aut Robert A. Pouliot verfasserin aut Michael M. Li verfasserin aut Peter M. Minneci verfasserin aut Zhenghong Tan verfasserin aut Kimberly Shontz verfasserin aut Jed Johnson verfasserin aut Susan D. Reynolds verfasserin aut Christopher K. Breuer verfasserin aut Daniel J. Weiss verfasserin aut Tendy Chiang verfasserin aut In Bioengineering MDPI AG, 2014 8(2021), 8, p 116 (DE-627)774814020 (DE-600)2746191-9 23065354 nnns volume:8 year:2021 number:8, p 116 https://doi.org/10.3390/bioengineering8080116 kostenfrei https://doaj.org/article/eb93d533b79e4011a9d32ee721dd0c94 kostenfrei https://www.mdpi.com/2306-5354/8/8/116 kostenfrei https://doaj.org/toc/2306-5354 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 8, p 116 |
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10.3390/bioengineering8080116 doi (DE-627)DOAJ057556873 (DE-599)DOAJeb93d533b79e4011a9d32ee721dd0c94 DE-627 ger DE-627 rakwb eng QH301-705.5 Lumei Liu verfasserin aut Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning Technology T Biology (General) Sayali Dharmadhikari verfasserin aut Robert A. Pouliot verfasserin aut Michael M. Li verfasserin aut Peter M. Minneci verfasserin aut Zhenghong Tan verfasserin aut Kimberly Shontz verfasserin aut Jed Johnson verfasserin aut Susan D. Reynolds verfasserin aut Christopher K. Breuer verfasserin aut Daniel J. Weiss verfasserin aut Tendy Chiang verfasserin aut In Bioengineering MDPI AG, 2014 8(2021), 8, p 116 (DE-627)774814020 (DE-600)2746191-9 23065354 nnns volume:8 year:2021 number:8, p 116 https://doi.org/10.3390/bioengineering8080116 kostenfrei https://doaj.org/article/eb93d533b79e4011a9d32ee721dd0c94 kostenfrei https://www.mdpi.com/2306-5354/8/8/116 kostenfrei https://doaj.org/toc/2306-5354 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 8, p 116 |
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10.3390/bioengineering8080116 doi (DE-627)DOAJ057556873 (DE-599)DOAJeb93d533b79e4011a9d32ee721dd0c94 DE-627 ger DE-627 rakwb eng QH301-705.5 Lumei Liu verfasserin aut Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning Technology T Biology (General) Sayali Dharmadhikari verfasserin aut Robert A. Pouliot verfasserin aut Michael M. Li verfasserin aut Peter M. Minneci verfasserin aut Zhenghong Tan verfasserin aut Kimberly Shontz verfasserin aut Jed Johnson verfasserin aut Susan D. Reynolds verfasserin aut Christopher K. Breuer verfasserin aut Daniel J. Weiss verfasserin aut Tendy Chiang verfasserin aut In Bioengineering MDPI AG, 2014 8(2021), 8, p 116 (DE-627)774814020 (DE-600)2746191-9 23065354 nnns volume:8 year:2021 number:8, p 116 https://doi.org/10.3390/bioengineering8080116 kostenfrei https://doaj.org/article/eb93d533b79e4011a9d32ee721dd0c94 kostenfrei https://www.mdpi.com/2306-5354/8/8/116 kostenfrei https://doaj.org/toc/2306-5354 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 8, p 116 |
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10.3390/bioengineering8080116 doi (DE-627)DOAJ057556873 (DE-599)DOAJeb93d533b79e4011a9d32ee721dd0c94 DE-627 ger DE-627 rakwb eng QH301-705.5 Lumei Liu verfasserin aut Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning Technology T Biology (General) Sayali Dharmadhikari verfasserin aut Robert A. Pouliot verfasserin aut Michael M. Li verfasserin aut Peter M. Minneci verfasserin aut Zhenghong Tan verfasserin aut Kimberly Shontz verfasserin aut Jed Johnson verfasserin aut Susan D. Reynolds verfasserin aut Christopher K. Breuer verfasserin aut Daniel J. Weiss verfasserin aut Tendy Chiang verfasserin aut In Bioengineering MDPI AG, 2014 8(2021), 8, p 116 (DE-627)774814020 (DE-600)2746191-9 23065354 nnns volume:8 year:2021 number:8, p 116 https://doi.org/10.3390/bioengineering8080116 kostenfrei https://doaj.org/article/eb93d533b79e4011a9d32ee721dd0c94 kostenfrei https://www.mdpi.com/2306-5354/8/8/116 kostenfrei https://doaj.org/toc/2306-5354 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 8, p 116 |
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QH301-705.5 Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning |
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Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings |
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Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. |
abstractGer |
Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. |
abstract_unstemmed |
Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo. |
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In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. 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