Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings

Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (sy...
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Autor*in:	Lumei Liu [verfasserIn] Sayali Dharmadhikari [verfasserIn] Robert A. Pouliot [verfasserIn] Michael M. Li [verfasserIn] Peter M. Minneci [verfasserIn] Zhenghong Tan [verfasserIn] Kimberly Shontz [verfasserIn] Jed Johnson [verfasserIn] Susan D. Reynolds [verfasserIn] Christopher K. Breuer [verfasserIn] Daniel J. Weiss [verfasserIn] Tendy Chiang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning

Übergeordnetes Werk:	In: Bioengineering - MDPI AG, 2014, 8(2021), 8, p 116
Übergeordnetes Werk:	volume:8 ; year:2021 ; number:8, p 116

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/bioengineering8080116

Katalog-ID:	DOAJ057556873

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callnumber-first	Q - Science
author	Lumei Liu
spellingShingle	Lumei Liu misc QH301-705.5 misc cell viability misc biocompatibility misc extracellular matrix coating misc synthetic tracheal graft misc tracheal regeneration misc electrospinning misc Technology misc T misc Biology (General) Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings
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topic_title	QH301-705.5 Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings cell viability biocompatibility extracellular matrix coating synthetic tracheal graft tracheal regeneration electrospinning
topic	misc QH301-705.5 misc cell viability misc biocompatibility misc extracellular matrix coating misc synthetic tracheal graft misc tracheal regeneration misc electrospinning misc Technology misc T misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc cell viability misc biocompatibility misc extracellular matrix coating misc synthetic tracheal graft misc tracheal regeneration misc electrospinning misc Technology misc T misc Biology (General)
topic_browse	misc QH301-705.5 misc cell viability misc biocompatibility misc extracellular matrix coating misc synthetic tracheal graft misc tracheal regeneration misc electrospinning misc Technology misc T misc Biology (General)
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title	Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings
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title_full	Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings
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author_browse	Lumei Liu Sayali Dharmadhikari Robert A. Pouliot Michael M. Li Peter M. Minneci Zhenghong Tan Kimberly Shontz Jed Johnson Susan D. Reynolds Christopher K. Breuer Daniel J. Weiss Tendy Chiang
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title_sort	modulation of synthetic tracheal grafts with extracellular matrix coatings
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title_auth	Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings
abstract	Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo.
abstractGer	Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo.
abstract_unstemmed	Synthetic scaffolds for the repair of long-segment tracheal defects are hindered by insufficient biocompatibility and poor graft epithelialization. In this study, we determined if extracellular matrix (ECM) coatings improved the biocompatibility and epithelialization of synthetic tracheal grafts (syn-TG). Porcine and human ECM substrates (pECM and hECM) were created through the decellularization and lyophilization of lung tissue. Four concentrations of pECM and hECM coatings on syn-TG were characterized for their effects on scaffold morphologies and on in vitro cell viability and growth. Uncoated and ECM-coated syn-TG were subsequently evaluated in vivo through the orthotopic implantation of segmental grafts or patches. These studies demonstrated that ECM coatings were not cytotoxic and, enhanced the in vitro cell viability and growth on syn-TG in a dose-dependent manner. Mass spectrometry demonstrated that fibrillin, collagen, laminin, and nephronectin were the predominant ECM components transferred onto scaffolds. The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. Overall, these findings demonstrated that ECM coatings improve the seeded cell biocompatibility of synthetic scaffolds in vitro; however, they do not improve graft epithelialization in vivo.
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container_issue	8, p 116
title_short	Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings
url	https://doi.org/10.3390/bioengineering8080116 https://doaj.org/article/eb93d533b79e4011a9d32ee721dd0c94 https://www.mdpi.com/2306-5354/8/8/116 https://doaj.org/toc/2306-5354
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author2	Sayali Dharmadhikari Robert A. Pouliot Michael M. Li Peter M. Minneci Zhenghong Tan Kimberly Shontz Jed Johnson Susan D. Reynolds Christopher K. Breuer Daniel J. Weiss Tendy Chiang
author2Str	Sayali Dharmadhikari Robert A. Pouliot Michael M. Li Peter M. Minneci Zhenghong Tan Kimberly Shontz Jed Johnson Susan D. Reynolds Christopher K. Breuer Daniel J. Weiss Tendy Chiang
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up_date	2024-07-04T02:07:18.178Z
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The in vivo results exhibited similar robust epithelialization of uncoated and coated syn-TG patches; however, the epithelialization remained poor with either uncoated or coated scaffolds in the segmental replacement models. 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