Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile

Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obta...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Hamid Heidari [verfasserIn] Hadi Sedigh Ebrahim-Saraie [verfasserIn] Ali Amanati [verfasserIn] Mohammad Motamedifar [verfasserIn] Nahal Hadi [verfasserIn] Abdollah Bazargani [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance

Übergeordnetes Werk:	In: Iranian Journal of Basic Medical Sciences - Mashhad University of Medical Sciences, 2009, 22(2019), 7, Seite 813-819
Übergeordnetes Werk:	volume:22 ; year:2019 ; number:7 ; pages:813-819

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.22038/ijbms.2019.35223.8390

Katalog-ID:	DOAJ057615896

Internformat


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245	1	0	\|a Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile
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520			\|a Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital.
650		4	\|a Clostridioides (Clostridium) difficile
650		4	\|a C. difficile infection
650		4	\|a Toxins
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650		4	\|a Antibiotic resistance
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author_variant	h h hh h s e s hses a a aa m m mm n h nh a b ab
matchkey_str	article:20083874:2019----::oipoieadniirbarssacptenaogoieiciiaioaeocot
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publishDate	2019
allfields	10.22038/ijbms.2019.35223.8390 doi (DE-627)DOAJ057615896 (DE-599)DOAJf33aaa63a91649759cfc58ba078683f3 DE-627 ger DE-627 rakwb eng Hamid Heidari verfasserin aut Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital. Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance Medicine R Hadi Sedigh Ebrahim-Saraie verfasserin aut Ali Amanati verfasserin aut Mohammad Motamedifar verfasserin aut Nahal Hadi verfasserin aut Abdollah Bazargani verfasserin aut In Iranian Journal of Basic Medical Sciences Mashhad University of Medical Sciences, 2009 22(2019), 7, Seite 813-819 (DE-627)602537185 (DE-600)2500485-2 20083874 nnns volume:22 year:2019 number:7 pages:813-819 https://doi.org/10.22038/ijbms.2019.35223.8390 kostenfrei https://doaj.org/article/f33aaa63a91649759cfc58ba078683f3 kostenfrei http://ijbms.mums.ac.ir/article_13015_2f36447812c3ad3bd2e5f26afb1a936d.pdf kostenfrei https://doaj.org/toc/2008-3866 Journal toc kostenfrei https://doaj.org/toc/2008-3874 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2019 7 813-819
spelling	10.22038/ijbms.2019.35223.8390 doi (DE-627)DOAJ057615896 (DE-599)DOAJf33aaa63a91649759cfc58ba078683f3 DE-627 ger DE-627 rakwb eng Hamid Heidari verfasserin aut Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital. Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance Medicine R Hadi Sedigh Ebrahim-Saraie verfasserin aut Ali Amanati verfasserin aut Mohammad Motamedifar verfasserin aut Nahal Hadi verfasserin aut Abdollah Bazargani verfasserin aut In Iranian Journal of Basic Medical Sciences Mashhad University of Medical Sciences, 2009 22(2019), 7, Seite 813-819 (DE-627)602537185 (DE-600)2500485-2 20083874 nnns volume:22 year:2019 number:7 pages:813-819 https://doi.org/10.22038/ijbms.2019.35223.8390 kostenfrei https://doaj.org/article/f33aaa63a91649759cfc58ba078683f3 kostenfrei http://ijbms.mums.ac.ir/article_13015_2f36447812c3ad3bd2e5f26afb1a936d.pdf kostenfrei https://doaj.org/toc/2008-3866 Journal toc kostenfrei https://doaj.org/toc/2008-3874 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2019 7 813-819
allfields_unstemmed	10.22038/ijbms.2019.35223.8390 doi (DE-627)DOAJ057615896 (DE-599)DOAJf33aaa63a91649759cfc58ba078683f3 DE-627 ger DE-627 rakwb eng Hamid Heidari verfasserin aut Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital. Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance Medicine R Hadi Sedigh Ebrahim-Saraie verfasserin aut Ali Amanati verfasserin aut Mohammad Motamedifar verfasserin aut Nahal Hadi verfasserin aut Abdollah Bazargani verfasserin aut In Iranian Journal of Basic Medical Sciences Mashhad University of Medical Sciences, 2009 22(2019), 7, Seite 813-819 (DE-627)602537185 (DE-600)2500485-2 20083874 nnns volume:22 year:2019 number:7 pages:813-819 https://doi.org/10.22038/ijbms.2019.35223.8390 kostenfrei https://doaj.org/article/f33aaa63a91649759cfc58ba078683f3 kostenfrei http://ijbms.mums.ac.ir/article_13015_2f36447812c3ad3bd2e5f26afb1a936d.pdf kostenfrei https://doaj.org/toc/2008-3866 Journal toc kostenfrei https://doaj.org/toc/2008-3874 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2019 7 813-819
allfieldsGer	10.22038/ijbms.2019.35223.8390 doi (DE-627)DOAJ057615896 (DE-599)DOAJf33aaa63a91649759cfc58ba078683f3 DE-627 ger DE-627 rakwb eng Hamid Heidari verfasserin aut Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital. Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance Medicine R Hadi Sedigh Ebrahim-Saraie verfasserin aut Ali Amanati verfasserin aut Mohammad Motamedifar verfasserin aut Nahal Hadi verfasserin aut Abdollah Bazargani verfasserin aut In Iranian Journal of Basic Medical Sciences Mashhad University of Medical Sciences, 2009 22(2019), 7, Seite 813-819 (DE-627)602537185 (DE-600)2500485-2 20083874 nnns volume:22 year:2019 number:7 pages:813-819 https://doi.org/10.22038/ijbms.2019.35223.8390 kostenfrei https://doaj.org/article/f33aaa63a91649759cfc58ba078683f3 kostenfrei http://ijbms.mums.ac.ir/article_13015_2f36447812c3ad3bd2e5f26afb1a936d.pdf kostenfrei https://doaj.org/toc/2008-3866 Journal toc kostenfrei https://doaj.org/toc/2008-3874 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2019 7 813-819
allfieldsSound	10.22038/ijbms.2019.35223.8390 doi (DE-627)DOAJ057615896 (DE-599)DOAJf33aaa63a91649759cfc58ba078683f3 DE-627 ger DE-627 rakwb eng Hamid Heidari verfasserin aut Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital. Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance Medicine R Hadi Sedigh Ebrahim-Saraie verfasserin aut Ali Amanati verfasserin aut Mohammad Motamedifar verfasserin aut Nahal Hadi verfasserin aut Abdollah Bazargani verfasserin aut In Iranian Journal of Basic Medical Sciences Mashhad University of Medical Sciences, 2009 22(2019), 7, Seite 813-819 (DE-627)602537185 (DE-600)2500485-2 20083874 nnns volume:22 year:2019 number:7 pages:813-819 https://doi.org/10.22038/ijbms.2019.35223.8390 kostenfrei https://doaj.org/article/f33aaa63a91649759cfc58ba078683f3 kostenfrei http://ijbms.mums.ac.ir/article_13015_2f36447812c3ad3bd2e5f26afb1a936d.pdf kostenfrei https://doaj.org/toc/2008-3866 Journal toc kostenfrei https://doaj.org/toc/2008-3874 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2019 7 813-819
language	English
source	In Iranian Journal of Basic Medical Sciences 22(2019), 7, Seite 813-819 volume:22 year:2019 number:7 pages:813-819
sourceStr	In Iranian Journal of Basic Medical Sciences 22(2019), 7, Seite 813-819 volume:22 year:2019 number:7 pages:813-819
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance Medicine R
isfreeaccess_bool	true
container_title	Iranian Journal of Basic Medical Sciences
authorswithroles_txt_mv	Hamid Heidari @@aut@@ Hadi Sedigh Ebrahim-Saraie @@aut@@ Ali Amanati @@aut@@ Mohammad Motamedifar @@aut@@ Nahal Hadi @@aut@@ Abdollah Bazargani @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	602537185
id	DOAJ057615896
language_de	englisch
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author	Hamid Heidari
spellingShingle	Hamid Heidari misc Clostridioides (Clostridium) difficile misc C. difficile infection misc Toxins misc CDT misc Antibiotic resistance misc Medicine misc R Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile
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topic_title	Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile Clostridioides (Clostridium) difficile C. difficile infection Toxins CDT Antibiotic resistance
topic	misc Clostridioides (Clostridium) difficile misc C. difficile infection misc Toxins misc CDT misc Antibiotic resistance misc Medicine misc R
topic_unstemmed	misc Clostridioides (Clostridium) difficile misc C. difficile infection misc Toxins misc CDT misc Antibiotic resistance misc Medicine misc R
topic_browse	misc Clostridioides (Clostridium) difficile misc C. difficile infection misc Toxins misc CDT misc Antibiotic resistance misc Medicine misc R
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title	Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile
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title_full	Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile
author_sort	Hamid Heidari
journal	Iranian Journal of Basic Medical Sciences
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author_browse	Hamid Heidari Hadi Sedigh Ebrahim-Saraie Ali Amanati Mohammad Motamedifar Nahal Hadi Abdollah Bazargani
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title_sort	toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of clostridioides (clostridium) difficile
title_auth	Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile
abstract	Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital.
abstractGer	Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital.
abstract_unstemmed	Objective(s): Clostridioides (Clostridium) difficile infection as a healthcare-associated infection can cause life-threatening infectious diarrhea in hospitalized patients. The aim of this study was to investigate the toxin profiles and antimicrobial resistance patterns of C. difficile isolates obtained from hospitalized patients in Shiraz, Iran.Materials and Methods: This study was performed on 45 toxigenic C. difficile isolates. Determination of toxin profiles was done using polymerase chain reaction method. Antimicrobial susceptibility to vancomycin, metronidazole, clindamycin, tetracycline, moxifloxacin, and chloramphenicol was determined by the agar dilution method. The genes encoding antibiotic resistance were detected by the standard procedures.Results: The most frequent toxin profile was tcdA+, tcdB+, cdtAˉ, cdtBˉ (82.2%), and only one isolate harboured all toxin associated genes (tcdA+, tcdB+, cdtA+, cdtB+) (2.2%). The genes encoding CDT (binary toxin) were also found in six (13.3%) isolates. Resistance to tetracycline, clindamycin and moxifloxacin was observed in 66.7%, 60% and 42.2% of the isolates, respectively. None of the strains showed resistance to other antibiotics. The distribution of the ermB gene (the gene encoding resistance to clindamycin) was 57.8% and the tetM and tetW genes (the genes encoding resistance to tetracycline) were found in 62.2% and 13.3% of the isolates, respectively. The substitutions Thr82 to Ile in GyrA and Asp426 to Asn in GyrB were seen in moxifloxacin resistant isolates.Conclusion: Our data contributes to the present understanding of virulence and resistance traits amongst the isolates. Infection control strategies should be implemented carefully in order to curb the dissemination of C. difficile strains in hospital.
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title_short	Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile
url	https://doi.org/10.22038/ijbms.2019.35223.8390 https://doaj.org/article/f33aaa63a91649759cfc58ba078683f3 http://ijbms.mums.ac.ir/article_13015_2f36447812c3ad3bd2e5f26afb1a936d.pdf https://doaj.org/toc/2008-3866 https://doaj.org/toc/2008-3874
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author2	Hadi Sedigh Ebrahim-Saraie Ali Amanati Mohammad Motamedifar Nahal Hadi Abdollah Bazargani
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up_date	2024-07-04T02:19:20.737Z
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