Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate

Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study...
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Autor*in:	Shahram Nazarian [verfasserIn] Mohhamad Ali Arefpour [verfasserIn] Mohhamd Javad Bagheripour [verfasserIn] Golamreza Olad [verfasserIn]

Format:	E-Artikel
Sprache:	Persisch

Erschienen:	2014

Schlagwörter:	Cholera toxin Cholera enterotoxin subunit B (CtxB) Gene optimization Recombinant protein

Übergeordnetes Werk:	In: مجله دانشکده پزشکی اصفهان - Isfahan University of Medical Sciences, 2018, 32(2014), 279, Seite 378-387
Übergeordnetes Werk:	volume:32 ; year:2014 ; number:279 ; pages:378-387

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ057885028

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520			\|a Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose.
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allfields	(DE-627)DOAJ057885028 (DE-599)DOAJ1f6b5e92128f41c9b1c22033364884f7 DE-627 ger DE-627 rakwb per R5-920 Shahram Nazarian verfasserin aut Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose. Cholera toxin Cholera enterotoxin subunit B (CtxB) Gene optimization Recombinant protein Medicine R Medicine (General) Mohhamad Ali Arefpour verfasserin aut Mohhamd Javad Bagheripour verfasserin aut Golamreza Olad verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 32(2014), 279, Seite 378-387 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:32 year:2014 number:279 pages:378-387 https://doaj.org/article/1f6b5e92128f41c9b1c22033364884f7 kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/3777 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 32 2014 279 378-387
spelling	(DE-627)DOAJ057885028 (DE-599)DOAJ1f6b5e92128f41c9b1c22033364884f7 DE-627 ger DE-627 rakwb per R5-920 Shahram Nazarian verfasserin aut Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose. Cholera toxin Cholera enterotoxin subunit B (CtxB) Gene optimization Recombinant protein Medicine R Medicine (General) Mohhamad Ali Arefpour verfasserin aut Mohhamd Javad Bagheripour verfasserin aut Golamreza Olad verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 32(2014), 279, Seite 378-387 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:32 year:2014 number:279 pages:378-387 https://doaj.org/article/1f6b5e92128f41c9b1c22033364884f7 kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/3777 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 32 2014 279 378-387
allfields_unstemmed	(DE-627)DOAJ057885028 (DE-599)DOAJ1f6b5e92128f41c9b1c22033364884f7 DE-627 ger DE-627 rakwb per R5-920 Shahram Nazarian verfasserin aut Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose. Cholera toxin Cholera enterotoxin subunit B (CtxB) Gene optimization Recombinant protein Medicine R Medicine (General) Mohhamad Ali Arefpour verfasserin aut Mohhamd Javad Bagheripour verfasserin aut Golamreza Olad verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 32(2014), 279, Seite 378-387 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:32 year:2014 number:279 pages:378-387 https://doaj.org/article/1f6b5e92128f41c9b1c22033364884f7 kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/3777 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 32 2014 279 378-387
allfieldsGer	(DE-627)DOAJ057885028 (DE-599)DOAJ1f6b5e92128f41c9b1c22033364884f7 DE-627 ger DE-627 rakwb per R5-920 Shahram Nazarian verfasserin aut Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose. Cholera toxin Cholera enterotoxin subunit B (CtxB) Gene optimization Recombinant protein Medicine R Medicine (General) Mohhamad Ali Arefpour verfasserin aut Mohhamd Javad Bagheripour verfasserin aut Golamreza Olad verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 32(2014), 279, Seite 378-387 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:32 year:2014 number:279 pages:378-387 https://doaj.org/article/1f6b5e92128f41c9b1c22033364884f7 kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/3777 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 32 2014 279 378-387
allfieldsSound	(DE-627)DOAJ057885028 (DE-599)DOAJ1f6b5e92128f41c9b1c22033364884f7 DE-627 ger DE-627 rakwb per R5-920 Shahram Nazarian verfasserin aut Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose. Cholera toxin Cholera enterotoxin subunit B (CtxB) Gene optimization Recombinant protein Medicine R Medicine (General) Mohhamad Ali Arefpour verfasserin aut Mohhamd Javad Bagheripour verfasserin aut Golamreza Olad verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 32(2014), 279, Seite 378-387 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:32 year:2014 number:279 pages:378-387 https://doaj.org/article/1f6b5e92128f41c9b1c22033364884f7 kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/3777 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 32 2014 279 378-387
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Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. 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callnumber-first	R - Medicine
author	Shahram Nazarian
spellingShingle	Shahram Nazarian misc R5-920 misc Cholera toxin misc Cholera enterotoxin subunit B (CtxB) misc Gene optimization misc Recombinant protein misc Medicine misc R misc Medicine (General) Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate
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topic_title	R5-920 Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate Cholera toxin Cholera enterotoxin subunit B (CtxB) Gene optimization Recombinant protein
topic	misc R5-920 misc Cholera toxin misc Cholera enterotoxin subunit B (CtxB) misc Gene optimization misc Recombinant protein misc Medicine misc R misc Medicine (General)
topic_unstemmed	misc R5-920 misc Cholera toxin misc Cholera enterotoxin subunit B (CtxB) misc Gene optimization misc Recombinant protein misc Medicine misc R misc Medicine (General)
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title	Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate
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title_full	Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate
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author_browse	Shahram Nazarian Mohhamad Ali Arefpour Mohhamd Javad Bagheripour Golamreza Olad
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title_sort	bioinformatical study and evaluation of expression of cholera toxin subunit b optimized gene as a vaccine candidate
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title_auth	Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate
abstract	Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose.
abstractGer	Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose.
abstract_unstemmed	Background: Cholera is a lethal diarrheal disease caused by Vibrio cholerae. Cholera toxin is the major virulence factor in pathogenesis of Vibrio cholerae. The B subunit of the enterotoxin, which is responsible for toxin binding to eukaryotic cells, has immunogenic properties. The aim of this study was bioinformatic investigation and production of recombinant cholera toxin subunit B (CtxB). Methods: CtxB gene was analysed for rare codons and gene optimization was performed using optimization software. Recombinant pET28a/ctxb plasmid was transformed to E. coli BL21 DE3 and expression was induced with Isopropyl β-D-1-thiogalactopyranoside (IPTG). The protein expression was evaluated using Sodium dodecyl sulphate- Polyacrylamide gel electrophoresis (SDS-PAGE) and Western Blotting analysis. The recombinant protein was purified using Nickel-Nitrilotriacetic acid (Ni–NTA) affinity chromatography. Findings: Codon adaptation index (CAI) on the native gene was 0.61, while the optimized sequence had a CAI of 0.92. Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. The protein could be produced in capsulated form for oral immunization purpose.
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title_short	Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate
url	https://doaj.org/article/1f6b5e92128f41c9b1c22033364884f7 http://jims.mui.ac.ir/index.php/jims/article/view/3777 https://doaj.org/toc/1027-7595 https://doaj.org/toc/1735-854X
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author2	Mohhamad Ali Arefpour Mohhamd Javad Bagheripour Golamreza Olad
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Percentage of codon having high-frequency distribution was improved to 67%. Restriction analysis confirmed cloning of the CtxB gene into pET28a vector. Western blotting showed specific reactivity of recombinant protein with anti-CTX antibody. The total yield of purified protein was 9 mg/l. Conclusion: The results indicated that CtxB gene optimization is a useful approach to high-level expression of recombinant protein. 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Bioinformatical Study and Evaluation of Expression of Cholera Toxin Subunit B Optimized Gene as a Vaccine Candidate

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