TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers

Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies in...
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Autor*in:	Glauco Akelinghton Freire Vitiello [verfasserIn] Marla Karine Amarante [verfasserIn] Jefferson Crespigio [verfasserIn] Bruna Karina Banin Hirata [verfasserIn] Nathalia de Sousa Pereira [verfasserIn] Karen Brajão de Oliveira [verfasserIn] Roberta Losi Guembarovski [verfasserIn] Maria Angelica Ehara Watanabe [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms

Übergeordnetes Werk:	In: Surgical and Experimental Pathology - BMC, 2019, 4(2021), 1, Seite 13
Übergeordnetes Werk:	volume:4 ; year:2021 ; number:1 ; pages:13

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s42047-021-00097-0

Katalog-ID:	DOAJ057936544

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520			\|a Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups.
650		4	\|a Transforming growth factor beta
650		4	\|a Breast neoplasm
650		4	\|a Immunohistochemistry
650		4	\|a Biomarkers
650		4	\|a Prognosis
650		4	\|a Polymorphisms
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author_variant	g a f v gafv m k a mka j c jc b k b h bkbh n d s p ndsp k b d o kbdo r l g rlg m a e w maew
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allfields	10.1186/s42047-021-00097-0 doi (DE-627)DOAJ057936544 (DE-599)DOAJ8bad43f3d1824ec2b108ab4a5c597844 DE-627 ger DE-627 rakwb eng RD1-811 RB1-214 Glauco Akelinghton Freire Vitiello verfasserin aut TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups. Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms Surgery Pathology Marla Karine Amarante verfasserin aut Jefferson Crespigio verfasserin aut Bruna Karina Banin Hirata verfasserin aut Nathalia de Sousa Pereira verfasserin aut Karen Brajão de Oliveira verfasserin aut Roberta Losi Guembarovski verfasserin aut Maria Angelica Ehara Watanabe verfasserin aut In Surgical and Experimental Pathology BMC, 2019 4(2021), 1, Seite 13 (DE-627)1040711715 25208454 nnns volume:4 year:2021 number:1 pages:13 https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/article/8bad43f3d1824ec2b108ab4a5c597844 kostenfrei https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/toc/2520-8454 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2021 1 13
spelling	10.1186/s42047-021-00097-0 doi (DE-627)DOAJ057936544 (DE-599)DOAJ8bad43f3d1824ec2b108ab4a5c597844 DE-627 ger DE-627 rakwb eng RD1-811 RB1-214 Glauco Akelinghton Freire Vitiello verfasserin aut TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups. Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms Surgery Pathology Marla Karine Amarante verfasserin aut Jefferson Crespigio verfasserin aut Bruna Karina Banin Hirata verfasserin aut Nathalia de Sousa Pereira verfasserin aut Karen Brajão de Oliveira verfasserin aut Roberta Losi Guembarovski verfasserin aut Maria Angelica Ehara Watanabe verfasserin aut In Surgical and Experimental Pathology BMC, 2019 4(2021), 1, Seite 13 (DE-627)1040711715 25208454 nnns volume:4 year:2021 number:1 pages:13 https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/article/8bad43f3d1824ec2b108ab4a5c597844 kostenfrei https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/toc/2520-8454 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2021 1 13
allfields_unstemmed	10.1186/s42047-021-00097-0 doi (DE-627)DOAJ057936544 (DE-599)DOAJ8bad43f3d1824ec2b108ab4a5c597844 DE-627 ger DE-627 rakwb eng RD1-811 RB1-214 Glauco Akelinghton Freire Vitiello verfasserin aut TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups. Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms Surgery Pathology Marla Karine Amarante verfasserin aut Jefferson Crespigio verfasserin aut Bruna Karina Banin Hirata verfasserin aut Nathalia de Sousa Pereira verfasserin aut Karen Brajão de Oliveira verfasserin aut Roberta Losi Guembarovski verfasserin aut Maria Angelica Ehara Watanabe verfasserin aut In Surgical and Experimental Pathology BMC, 2019 4(2021), 1, Seite 13 (DE-627)1040711715 25208454 nnns volume:4 year:2021 number:1 pages:13 https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/article/8bad43f3d1824ec2b108ab4a5c597844 kostenfrei https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/toc/2520-8454 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2021 1 13
allfieldsGer	10.1186/s42047-021-00097-0 doi (DE-627)DOAJ057936544 (DE-599)DOAJ8bad43f3d1824ec2b108ab4a5c597844 DE-627 ger DE-627 rakwb eng RD1-811 RB1-214 Glauco Akelinghton Freire Vitiello verfasserin aut TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups. Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms Surgery Pathology Marla Karine Amarante verfasserin aut Jefferson Crespigio verfasserin aut Bruna Karina Banin Hirata verfasserin aut Nathalia de Sousa Pereira verfasserin aut Karen Brajão de Oliveira verfasserin aut Roberta Losi Guembarovski verfasserin aut Maria Angelica Ehara Watanabe verfasserin aut In Surgical and Experimental Pathology BMC, 2019 4(2021), 1, Seite 13 (DE-627)1040711715 25208454 nnns volume:4 year:2021 number:1 pages:13 https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/article/8bad43f3d1824ec2b108ab4a5c597844 kostenfrei https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/toc/2520-8454 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2021 1 13
allfieldsSound	10.1186/s42047-021-00097-0 doi (DE-627)DOAJ057936544 (DE-599)DOAJ8bad43f3d1824ec2b108ab4a5c597844 DE-627 ger DE-627 rakwb eng RD1-811 RB1-214 Glauco Akelinghton Freire Vitiello verfasserin aut TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups. Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms Surgery Pathology Marla Karine Amarante verfasserin aut Jefferson Crespigio verfasserin aut Bruna Karina Banin Hirata verfasserin aut Nathalia de Sousa Pereira verfasserin aut Karen Brajão de Oliveira verfasserin aut Roberta Losi Guembarovski verfasserin aut Maria Angelica Ehara Watanabe verfasserin aut In Surgical and Experimental Pathology BMC, 2019 4(2021), 1, Seite 13 (DE-627)1040711715 25208454 nnns volume:4 year:2021 number:1 pages:13 https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/article/8bad43f3d1824ec2b108ab4a5c597844 kostenfrei https://doi.org/10.1186/s42047-021-00097-0 kostenfrei https://doaj.org/toc/2520-8454 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2021 1 13
language	English
source	In Surgical and Experimental Pathology 4(2021), 1, Seite 13 volume:4 year:2021 number:1 pages:13
sourceStr	In Surgical and Experimental Pathology 4(2021), 1, Seite 13 volume:4 year:2021 number:1 pages:13
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms Surgery Pathology
isfreeaccess_bool	true
container_title	Surgical and Experimental Pathology
authorswithroles_txt_mv	Glauco Akelinghton Freire Vitiello @@aut@@ Marla Karine Amarante @@aut@@ Jefferson Crespigio @@aut@@ Bruna Karina Banin Hirata @@aut@@ Nathalia de Sousa Pereira @@aut@@ Karen Brajão de Oliveira @@aut@@ Roberta Losi Guembarovski @@aut@@ Maria Angelica Ehara Watanabe @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	1040711715
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callnumber-first	R - Medicine
author	Glauco Akelinghton Freire Vitiello
spellingShingle	Glauco Akelinghton Freire Vitiello misc RD1-811 misc RB1-214 misc Transforming growth factor beta misc Breast neoplasm misc Immunohistochemistry misc Biomarkers misc Prognosis misc Polymorphisms misc Surgery misc Pathology TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers
authorStr	Glauco Akelinghton Freire Vitiello
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topic_title	RD1-811 RB1-214 TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers Transforming growth factor beta Breast neoplasm Immunohistochemistry Biomarkers Prognosis Polymorphisms
topic	misc RD1-811 misc RB1-214 misc Transforming growth factor beta misc Breast neoplasm misc Immunohistochemistry misc Biomarkers misc Prognosis misc Polymorphisms misc Surgery misc Pathology
topic_unstemmed	misc RD1-811 misc RB1-214 misc Transforming growth factor beta misc Breast neoplasm misc Immunohistochemistry misc Biomarkers misc Prognosis misc Polymorphisms misc Surgery misc Pathology
topic_browse	misc RD1-811 misc RB1-214 misc Transforming growth factor beta misc Breast neoplasm misc Immunohistochemistry misc Biomarkers misc Prognosis misc Polymorphisms misc Surgery misc Pathology
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title	TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers
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title_full	TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers
author_sort	Glauco Akelinghton Freire Vitiello
journal	Surgical and Experimental Pathology
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author_browse	Glauco Akelinghton Freire Vitiello Marla Karine Amarante Jefferson Crespigio Bruna Karina Banin Hirata Nathalia de Sousa Pereira Karen Brajão de Oliveira Roberta Losi Guembarovski Maria Angelica Ehara Watanabe
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title_sort	tgfβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in er-positive and p53-negative cancers
callnumber	RD1-811
title_auth	TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers
abstract	Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups.
abstractGer	Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups.
abstract_unstemmed	Abstract Background TGFβ signaling exerts context-specific effects in breast cancer (BC) pathogenesis and single nucleotide polymorphisms (SNPs) in TGFβ-signaling components play a role in the genetic control of their expression and in BC susceptibility and clinical presentation. However, studies investigating the association between the TGFβ-signaling molecules and BC prognosis rarely considered disease subtypes and SNPs. Therefore, the present study aimed to evaluate the expression of TGFβ-signaling components in BC tissue from patients with available data regarding TGFB1 and TGFBR2 SNPs and plasmatic TGFβ1 levels. Methods Immunostaining for TGFβ1, TGFβRII and phosphorylated (p)-SMAD2/3 was investigated in primary tumor tissue from 34 patients with luminal-B-HER2+ (LB-HER2), HER2-enriched (HER2) and triple negative (TN) BC subtypes genotyped for TGFB1 (rs1800468, rs1800469, rs1800470 and rs1800471) and TGFBR2 (rs3087465) SNPs. Results Strong positive correlations were observed between TGFβ1, TGFβRII and p-SMAD2/3 in tumor tissue, and an inverse correlation was observed between intratumor and plasmatic TGFβ1 levels in TN BCs. In LB-HER2+ tumors, p-SMAD2/3 was associated with older age at diagnosis and inversely correlated with p53 staining and lymph-node metastasis, while tumor-size negatively correlated with TGFβ1 and TGFβRII in this BC subgroup. Also, in p53-negative BCs, tumor size and Ki67 negatively correlated with both TGFβ1, TGFβRII and p-SMAD2/3. No correlation was found between SNPs and TGFβ1-signaling components expression. Conclusion TGFβ1 canonical signaling is activated in approximately half of BCs, and correlation between TGFβ components indicate a paracrine activation, which may exert tumor suppressor effects in p53-negative or Luminal-B-HER2+ subgroups.
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title_short	TGFβ1 pathway components in breast cancer tissue from aggressive subtypes correlate with better prognostic parameters in ER-positive and p53-negative cancers
url	https://doi.org/10.1186/s42047-021-00097-0 https://doaj.org/article/8bad43f3d1824ec2b108ab4a5c597844 https://doaj.org/toc/2520-8454
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author2	Marla Karine Amarante Jefferson Crespigio Bruna Karina Banin Hirata Nathalia de Sousa Pereira Karen Brajão de Oliveira Roberta Losi Guembarovski Maria Angelica Ehara Watanabe
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