The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation

Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subset...
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Autor*in:	Ignacio I Wistuba [verfasserIn] Michael T Tetzlaff [verfasserIn] Carlo B Bifulco [verfasserIn] Katharina von Loga [verfasserIn] Sacha Gnjatic [verfasserIn] Scott J Rodig [verfasserIn] Keith E Steele [verfasserIn] Marlon C Rebelatto [verfasserIn] Ana Lako [verfasserIn] Guray Akturk [verfasserIn] Michael Angelo [verfasserIn] Shirley Greenbaum [verfasserIn] Noah F Greenwald [verfasserIn] Cyrus V Hedvat [verfasserIn] Travis J Hollmann [verfasserIn] Jonathan Juco [verfasserIn] Jaime Rodriguez-Canales [verfasserIn] Kurt A Schalper [verfasserIn] Edward C Stack [verfasserIn] Cláudia S Ferreira [verfasserIn] Konstanty Korski [verfasserIn] Emanuel Schenck [verfasserIn] Michael J Surace [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Übergeordnetes Werk:	In: Journal for ImmunoTherapy of Cancer - BMJ Publishing Group, 2013, 8(2020), 1
Übergeordnetes Werk:	volume:8 ; year:2020 ; number:1

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1136/jitc-2019-000155

Katalog-ID:	DOAJ058315047

Internformat


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520			\|a Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force.
653		0	\|a Neoplasms. Tumors. Oncology. Including cancer and carcinogens
700	0		\|a Michael T Tetzlaff \|e verfasserin \|4 aut
700	0		\|a Carlo B Bifulco \|e verfasserin \|4 aut
700	0		\|a Katharina von Loga \|e verfasserin \|4 aut
700	0		\|a Sacha Gnjatic \|e verfasserin \|4 aut
700	0		\|a Scott J Rodig \|e verfasserin \|4 aut
700	0		\|a Keith E Steele \|e verfasserin \|4 aut
700	0		\|a Marlon C Rebelatto \|e verfasserin \|4 aut
700	0		\|a Ana Lako \|e verfasserin \|4 aut
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700	0		\|a Cláudia S Ferreira \|e verfasserin \|4 aut
700	0		\|a Konstanty Korski \|e verfasserin \|4 aut
700	0		\|a Emanuel Schenck \|e verfasserin \|4 aut
700	0		\|a Michael J Surace \|e verfasserin \|4 aut
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author_variant	i i w iiw m t t mtt c b b cbb k v l kvl s g sg s j r sjr k e s kes m c r mcr a l al g a ga m a ma s g sg n f g nfg c v h cvh t j h tjh j j jj j r c jrc k a s kas e c s ecs c s f csf k k kk e s es m j s mjs
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allfields	10.1136/jitc-2019-000155 doi (DE-627)DOAJ058315047 (DE-599)DOAJaa359e762b4f41ddb084a9f360b5fc80 DE-627 ger DE-627 rakwb eng RC254-282 Ignacio I Wistuba verfasserin aut The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Michael T Tetzlaff verfasserin aut Carlo B Bifulco verfasserin aut Katharina von Loga verfasserin aut Sacha Gnjatic verfasserin aut Scott J Rodig verfasserin aut Keith E Steele verfasserin aut Marlon C Rebelatto verfasserin aut Ana Lako verfasserin aut Guray Akturk verfasserin aut Michael Angelo verfasserin aut Shirley Greenbaum verfasserin aut Noah F Greenwald verfasserin aut Cyrus V Hedvat verfasserin aut Travis J Hollmann verfasserin aut Jonathan Juco verfasserin aut Jaime Rodriguez-Canales verfasserin aut Kurt A Schalper verfasserin aut Edward C Stack verfasserin aut Cláudia S Ferreira verfasserin aut Konstanty Korski verfasserin aut Emanuel Schenck verfasserin aut Michael J Surace verfasserin aut In Journal for ImmunoTherapy of Cancer BMJ Publishing Group, 2013 8(2020), 1 (DE-627)750086335 (DE-600)2719863-7 20511426 nnns volume:8 year:2020 number:1 https://doi.org/10.1136/jitc-2019-000155 kostenfrei https://doaj.org/article/aa359e762b4f41ddb084a9f360b5fc80 kostenfrei https://jitc.bmj.com/content/8/1/e000155.full kostenfrei https://doaj.org/toc/2051-1426 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 1
spelling	10.1136/jitc-2019-000155 doi (DE-627)DOAJ058315047 (DE-599)DOAJaa359e762b4f41ddb084a9f360b5fc80 DE-627 ger DE-627 rakwb eng RC254-282 Ignacio I Wistuba verfasserin aut The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Michael T Tetzlaff verfasserin aut Carlo B Bifulco verfasserin aut Katharina von Loga verfasserin aut Sacha Gnjatic verfasserin aut Scott J Rodig verfasserin aut Keith E Steele verfasserin aut Marlon C Rebelatto verfasserin aut Ana Lako verfasserin aut Guray Akturk verfasserin aut Michael Angelo verfasserin aut Shirley Greenbaum verfasserin aut Noah F Greenwald verfasserin aut Cyrus V Hedvat verfasserin aut Travis J Hollmann verfasserin aut Jonathan Juco verfasserin aut Jaime Rodriguez-Canales verfasserin aut Kurt A Schalper verfasserin aut Edward C Stack verfasserin aut Cláudia S Ferreira verfasserin aut Konstanty Korski verfasserin aut Emanuel Schenck verfasserin aut Michael J Surace verfasserin aut In Journal for ImmunoTherapy of Cancer BMJ Publishing Group, 2013 8(2020), 1 (DE-627)750086335 (DE-600)2719863-7 20511426 nnns volume:8 year:2020 number:1 https://doi.org/10.1136/jitc-2019-000155 kostenfrei https://doaj.org/article/aa359e762b4f41ddb084a9f360b5fc80 kostenfrei https://jitc.bmj.com/content/8/1/e000155.full kostenfrei https://doaj.org/toc/2051-1426 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 1
allfields_unstemmed	10.1136/jitc-2019-000155 doi (DE-627)DOAJ058315047 (DE-599)DOAJaa359e762b4f41ddb084a9f360b5fc80 DE-627 ger DE-627 rakwb eng RC254-282 Ignacio I Wistuba verfasserin aut The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Michael T Tetzlaff verfasserin aut Carlo B Bifulco verfasserin aut Katharina von Loga verfasserin aut Sacha Gnjatic verfasserin aut Scott J Rodig verfasserin aut Keith E Steele verfasserin aut Marlon C Rebelatto verfasserin aut Ana Lako verfasserin aut Guray Akturk verfasserin aut Michael Angelo verfasserin aut Shirley Greenbaum verfasserin aut Noah F Greenwald verfasserin aut Cyrus V Hedvat verfasserin aut Travis J Hollmann verfasserin aut Jonathan Juco verfasserin aut Jaime Rodriguez-Canales verfasserin aut Kurt A Schalper verfasserin aut Edward C Stack verfasserin aut Cláudia S Ferreira verfasserin aut Konstanty Korski verfasserin aut Emanuel Schenck verfasserin aut Michael J Surace verfasserin aut In Journal for ImmunoTherapy of Cancer BMJ Publishing Group, 2013 8(2020), 1 (DE-627)750086335 (DE-600)2719863-7 20511426 nnns volume:8 year:2020 number:1 https://doi.org/10.1136/jitc-2019-000155 kostenfrei https://doaj.org/article/aa359e762b4f41ddb084a9f360b5fc80 kostenfrei https://jitc.bmj.com/content/8/1/e000155.full kostenfrei https://doaj.org/toc/2051-1426 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 1
allfieldsGer	10.1136/jitc-2019-000155 doi (DE-627)DOAJ058315047 (DE-599)DOAJaa359e762b4f41ddb084a9f360b5fc80 DE-627 ger DE-627 rakwb eng RC254-282 Ignacio I Wistuba verfasserin aut The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Michael T Tetzlaff verfasserin aut Carlo B Bifulco verfasserin aut Katharina von Loga verfasserin aut Sacha Gnjatic verfasserin aut Scott J Rodig verfasserin aut Keith E Steele verfasserin aut Marlon C Rebelatto verfasserin aut Ana Lako verfasserin aut Guray Akturk verfasserin aut Michael Angelo verfasserin aut Shirley Greenbaum verfasserin aut Noah F Greenwald verfasserin aut Cyrus V Hedvat verfasserin aut Travis J Hollmann verfasserin aut Jonathan Juco verfasserin aut Jaime Rodriguez-Canales verfasserin aut Kurt A Schalper verfasserin aut Edward C Stack verfasserin aut Cláudia S Ferreira verfasserin aut Konstanty Korski verfasserin aut Emanuel Schenck verfasserin aut Michael J Surace verfasserin aut In Journal for ImmunoTherapy of Cancer BMJ Publishing Group, 2013 8(2020), 1 (DE-627)750086335 (DE-600)2719863-7 20511426 nnns volume:8 year:2020 number:1 https://doi.org/10.1136/jitc-2019-000155 kostenfrei https://doaj.org/article/aa359e762b4f41ddb084a9f360b5fc80 kostenfrei https://jitc.bmj.com/content/8/1/e000155.full kostenfrei https://doaj.org/toc/2051-1426 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 1
allfieldsSound	10.1136/jitc-2019-000155 doi (DE-627)DOAJ058315047 (DE-599)DOAJaa359e762b4f41ddb084a9f360b5fc80 DE-627 ger DE-627 rakwb eng RC254-282 Ignacio I Wistuba verfasserin aut The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force. Neoplasms. Tumors. Oncology. Including cancer and carcinogens Michael T Tetzlaff verfasserin aut Carlo B Bifulco verfasserin aut Katharina von Loga verfasserin aut Sacha Gnjatic verfasserin aut Scott J Rodig verfasserin aut Keith E Steele verfasserin aut Marlon C Rebelatto verfasserin aut Ana Lako verfasserin aut Guray Akturk verfasserin aut Michael Angelo verfasserin aut Shirley Greenbaum verfasserin aut Noah F Greenwald verfasserin aut Cyrus V Hedvat verfasserin aut Travis J Hollmann verfasserin aut Jonathan Juco verfasserin aut Jaime Rodriguez-Canales verfasserin aut Kurt A Schalper verfasserin aut Edward C Stack verfasserin aut Cláudia S Ferreira verfasserin aut Konstanty Korski verfasserin aut Emanuel Schenck verfasserin aut Michael J Surace verfasserin aut In Journal for ImmunoTherapy of Cancer BMJ Publishing Group, 2013 8(2020), 1 (DE-627)750086335 (DE-600)2719863-7 20511426 nnns volume:8 year:2020 number:1 https://doi.org/10.1136/jitc-2019-000155 kostenfrei https://doaj.org/article/aa359e762b4f41ddb084a9f360b5fc80 kostenfrei https://jitc.bmj.com/content/8/1/e000155.full kostenfrei https://doaj.org/toc/2051-1426 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 1
language	English
source	In Journal for ImmunoTherapy of Cancer 8(2020), 1 volume:8 year:2020 number:1
sourceStr	In Journal for ImmunoTherapy of Cancer 8(2020), 1 volume:8 year:2020 number:1
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Neoplasms. Tumors. Oncology. Including cancer and carcinogens
isfreeaccess_bool	true
container_title	Journal for ImmunoTherapy of Cancer
authorswithroles_txt_mv	Ignacio I Wistuba @@aut@@ Michael T Tetzlaff @@aut@@ Carlo B Bifulco @@aut@@ Katharina von Loga @@aut@@ Sacha Gnjatic @@aut@@ Scott J Rodig @@aut@@ Keith E Steele @@aut@@ Marlon C Rebelatto @@aut@@ Ana Lako @@aut@@ Guray Akturk @@aut@@ Michael Angelo @@aut@@ Shirley Greenbaum @@aut@@ Noah F Greenwald @@aut@@ Cyrus V Hedvat @@aut@@ Travis J Hollmann @@aut@@ Jonathan Juco @@aut@@ Jaime Rodriguez-Canales @@aut@@ Kurt A Schalper @@aut@@ Edward C Stack @@aut@@ Cláudia S Ferreira @@aut@@ Konstanty Korski @@aut@@ Emanuel Schenck @@aut@@ Michael J Surace @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	750086335
id	DOAJ058315047
language_de	englisch
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callnumber-first	R - Medicine
author	Ignacio I Wistuba
spellingShingle	Ignacio I Wistuba misc RC254-282 misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
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topic_title	RC254-282 The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
topic	misc RC254-282 misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
topic_unstemmed	misc RC254-282 misc Neoplasms. Tumors. Oncology. Including cancer and carcinogens
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title	The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
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title_full	The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
author_sort	Ignacio I Wistuba
journal	Journal for ImmunoTherapy of Cancer
journalStr	Journal for ImmunoTherapy of Cancer
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author_browse	Ignacio I Wistuba Michael T Tetzlaff Carlo B Bifulco Katharina von Loga Sacha Gnjatic Scott J Rodig Keith E Steele Marlon C Rebelatto Ana Lako Guray Akturk Michael Angelo Shirley Greenbaum Noah F Greenwald Cyrus V Hedvat Travis J Hollmann Jonathan Juco Jaime Rodriguez-Canales Kurt A Schalper Edward C Stack Cláudia S Ferreira Konstanty Korski Emanuel Schenck Michael J Surace
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author-letter	Ignacio I Wistuba
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title_sort	society for immunotherapy of cancer statement on best practices for multiplex immunohistochemistry (ihc) and immunofluorescence (if) staining and validation
callnumber	RC254-282
title_auth	The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
abstract	Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force.
abstractGer	Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force.
abstract_unstemmed	Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that can be used to help quantify immune cell subsets, their functional state, and their spatial arrangement within the tumor microenvironment.Methods The Society for Immunotherapy of Cancer (SITC) convened a task force of pathologists and laboratory leaders from academic centers as well as experts from pharmaceutical and diagnostic companies to develop best practice guidelines for the optimization and validation of mIHC/mIF assays across platforms.Results Representative outputs and the advantages and disadvantages of mIHC/mIF approaches, such as multiplexed chromogenic IHC, multiplexed immunohistochemical consecutive staining on single slide, mIF (including multispectral approaches), tissue-based mass spectrometry, and digital spatial profiling are discussed.Conclusions mIHC/mIF technologies are becoming standard tools for biomarker studies and are likely to enter routine clinical practice in the near future. Careful assay optimization and validation will help ensure outputs are robust and comparable across laboratories as well as potentially across mIHC/mIF platforms. Quantitative image analysis of mIHC/mIF output and data management considerations will be addressed in a complementary manuscript from this task force.
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title_short	The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
url	https://doi.org/10.1136/jitc-2019-000155 https://doaj.org/article/aa359e762b4f41ddb084a9f360b5fc80 https://jitc.bmj.com/content/8/1/e000155.full https://doaj.org/toc/2051-1426
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author2	Michael T Tetzlaff Carlo B Bifulco Katharina von Loga Sacha Gnjatic Scott J Rodig Keith E Steele Marlon C Rebelatto Ana Lako Guray Akturk Michael Angelo Shirley Greenbaum Noah F Greenwald Cyrus V Hedvat Travis J Hollmann Jonathan Juco Jaime Rodriguez-Canales Kurt A Schalper Edward C Stack Cláudia S Ferreira Konstanty Korski Emanuel Schenck Michael J Surace
author2Str	Michael T Tetzlaff Carlo B Bifulco Katharina von Loga Sacha Gnjatic Scott J Rodig Keith E Steele Marlon C Rebelatto Ana Lako Guray Akturk Michael Angelo Shirley Greenbaum Noah F Greenwald Cyrus V Hedvat Travis J Hollmann Jonathan Juco Jaime Rodriguez-Canales Kurt A Schalper Edward C Stack Cláudia S Ferreira Konstanty Korski Emanuel Schenck Michael J Surace
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The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation

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