Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution

Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequenc...
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Autor*in:	Zhong Zheng GUI [verfasserIn] Bo Yeon KIM [verfasserIn] Kwang Sik LEE [verfasserIn] Ya Dong WEI [verfasserIn] Xijie GUO [verfasserIn] Hung Dae SOHN [verfasserIn] Byung Rae JIN [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Schlagwörter:	baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm

Übergeordnetes Werk:	In: European Journal of Entomology - Institute of Entomology, Biology Centre, Czech Academy of Science, 2021, 105(2008), 4, Seite 567-574
Übergeordnetes Werk:	volume:105 ; year:2008 ; number:4 ; pages:567-574

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.14411/eje.2008.076

Katalog-ID:	DOAJ05838703X

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520			\|a Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae.
650		4	\|a baculovirus expression vector
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allfields	10.14411/eje.2008.076 doi (DE-627)DOAJ05838703X (DE-599)DOAJd9648eabc01c471787f2132383d7949b DE-627 ger DE-627 rakwb eng QL1-991 Zhong Zheng GUI verfasserin aut Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae. baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm Zoology Bo Yeon KIM verfasserin aut Kwang Sik LEE verfasserin aut Ya Dong WEI verfasserin aut Xijie GUO verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 105(2008), 4, Seite 567-574 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:105 year:2008 number:4 pages:567-574 https://doi.org/10.14411/eje.2008.076 kostenfrei https://doaj.org/article/d9648eabc01c471787f2132383d7949b kostenfrei https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 105 2008 4 567-574
spelling	10.14411/eje.2008.076 doi (DE-627)DOAJ05838703X (DE-599)DOAJd9648eabc01c471787f2132383d7949b DE-627 ger DE-627 rakwb eng QL1-991 Zhong Zheng GUI verfasserin aut Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae. baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm Zoology Bo Yeon KIM verfasserin aut Kwang Sik LEE verfasserin aut Ya Dong WEI verfasserin aut Xijie GUO verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 105(2008), 4, Seite 567-574 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:105 year:2008 number:4 pages:567-574 https://doi.org/10.14411/eje.2008.076 kostenfrei https://doaj.org/article/d9648eabc01c471787f2132383d7949b kostenfrei https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 105 2008 4 567-574
allfields_unstemmed	10.14411/eje.2008.076 doi (DE-627)DOAJ05838703X (DE-599)DOAJd9648eabc01c471787f2132383d7949b DE-627 ger DE-627 rakwb eng QL1-991 Zhong Zheng GUI verfasserin aut Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae. baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm Zoology Bo Yeon KIM verfasserin aut Kwang Sik LEE verfasserin aut Ya Dong WEI verfasserin aut Xijie GUO verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 105(2008), 4, Seite 567-574 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:105 year:2008 number:4 pages:567-574 https://doi.org/10.14411/eje.2008.076 kostenfrei https://doaj.org/article/d9648eabc01c471787f2132383d7949b kostenfrei https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 105 2008 4 567-574
allfieldsGer	10.14411/eje.2008.076 doi (DE-627)DOAJ05838703X (DE-599)DOAJd9648eabc01c471787f2132383d7949b DE-627 ger DE-627 rakwb eng QL1-991 Zhong Zheng GUI verfasserin aut Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae. baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm Zoology Bo Yeon KIM verfasserin aut Kwang Sik LEE verfasserin aut Ya Dong WEI verfasserin aut Xijie GUO verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 105(2008), 4, Seite 567-574 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:105 year:2008 number:4 pages:567-574 https://doi.org/10.14411/eje.2008.076 kostenfrei https://doaj.org/article/d9648eabc01c471787f2132383d7949b kostenfrei https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 105 2008 4 567-574
allfieldsSound	10.14411/eje.2008.076 doi (DE-627)DOAJ05838703X (DE-599)DOAJd9648eabc01c471787f2132383d7949b DE-627 ger DE-627 rakwb eng QL1-991 Zhong Zheng GUI verfasserin aut Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae. baculovirus expression vector bombyx mori cdna cloning enzyme gene structure glutathione s-transferase silkworm Zoology Bo Yeon KIM verfasserin aut Kwang Sik LEE verfasserin aut Ya Dong WEI verfasserin aut Xijie GUO verfasserin aut Hung Dae SOHN verfasserin aut Byung Rae JIN verfasserin aut In European Journal of Entomology Institute of Entomology, Biology Centre, Czech Academy of Science, 2021 105(2008), 4, Seite 567-574 (DE-627)478976038 (DE-600)2176324-0 18028829 nnns volume:105 year:2008 number:4 pages:567-574 https://doi.org/10.14411/eje.2008.076 kostenfrei https://doaj.org/article/d9648eabc01c471787f2132383d7949b kostenfrei https://www.eje.cz/artkey/eje-200804-0004_Glutathione_S-transferases_from_the_larval_gut_of_the_silkworm_Bombyx_mori_cDNA_cloning_gene_structure_expre.php kostenfrei https://doaj.org/toc/1210-5759 Journal toc kostenfrei https://doaj.org/toc/1802-8829 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 105 2008 4 567-574
language	English
source	In European Journal of Entomology 105(2008), 4, Seite 567-574 volume:105 year:2008 number:4 pages:567-574
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spellingShingle	Zhong Zheng GUI misc QL1-991 misc baculovirus expression vector misc bombyx mori misc cdna cloning misc enzyme misc gene structure misc glutathione s-transferase misc silkworm misc Zoology Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution
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title	Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution
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title_sort	glutathione s-transferases from the larval gut of the silkworm bombyx mori: cdna cloning, gene structure, expression and distribution
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title_auth	Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution
abstract	Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae.
abstractGer	Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae.
abstract_unstemmed	Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae.
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title_short	Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution
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Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution

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