Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development
The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Meng Lin [verfasserIn] Susanne Matschi [verfasserIn] Miguel Vasquez [verfasserIn] James Chamness [verfasserIn] Nicholas Kaczmar [verfasserIn] Matheus Baseggio [verfasserIn] Michael Miller [verfasserIn] Ethan L. Stewart [verfasserIn] Pengfei Qiao [verfasserIn] Michael J. Scanlon [verfasserIn] Isabel Molina [verfasserIn] Laurie G. Smith [verfasserIn] Michael A. Gore [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: G3: Genes, Genomes, Genetics - Oxford University Press, 2012, 10(2020), 5, Seite 1671-1683 |
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| Übergeordnetes Werk: |
volume:10 ; year:2020 ; number:5 ; pages:1671-1683 |
| Links: |
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| DOI / URN: |
10.1534/g3.119.400884 |
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| Katalog-ID: |
DOAJ058418628 |
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| 520 | |a The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. | ||
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10.1534/g3.119.400884 doi (DE-627)DOAJ058418628 (DE-599)DOAJ5f190a0c498e44b98a8a018fd84b6993 DE-627 ger DE-627 rakwb eng QH426-470 Meng Lin verfasserin aut Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. cuticle cuticular conductance genome-wide association study rna sequencing whole-genome prediction Genetics Susanne Matschi verfasserin aut Miguel Vasquez verfasserin aut James Chamness verfasserin aut Nicholas Kaczmar verfasserin aut Matheus Baseggio verfasserin aut Michael Miller verfasserin aut Ethan L. Stewart verfasserin aut Pengfei Qiao verfasserin aut Michael J. Scanlon verfasserin aut Isabel Molina verfasserin aut Laurie G. Smith verfasserin aut Michael A. Gore verfasserin aut In G3: Genes, Genomes, Genetics Oxford University Press, 2012 10(2020), 5, Seite 1671-1683 (DE-627)668901071 (DE-600)2629978-1 21601836 nnns volume:10 year:2020 number:5 pages:1671-1683 https://doi.org/10.1534/g3.119.400884 kostenfrei https://doaj.org/article/5f190a0c498e44b98a8a018fd84b6993 kostenfrei http://g3journal.org/lookup/doi/10.1534/g3.119.400884 kostenfrei https://doaj.org/toc/2160-1836 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 5 1671-1683 |
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10.1534/g3.119.400884 doi (DE-627)DOAJ058418628 (DE-599)DOAJ5f190a0c498e44b98a8a018fd84b6993 DE-627 ger DE-627 rakwb eng QH426-470 Meng Lin verfasserin aut Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. cuticle cuticular conductance genome-wide association study rna sequencing whole-genome prediction Genetics Susanne Matschi verfasserin aut Miguel Vasquez verfasserin aut James Chamness verfasserin aut Nicholas Kaczmar verfasserin aut Matheus Baseggio verfasserin aut Michael Miller verfasserin aut Ethan L. Stewart verfasserin aut Pengfei Qiao verfasserin aut Michael J. Scanlon verfasserin aut Isabel Molina verfasserin aut Laurie G. Smith verfasserin aut Michael A. Gore verfasserin aut In G3: Genes, Genomes, Genetics Oxford University Press, 2012 10(2020), 5, Seite 1671-1683 (DE-627)668901071 (DE-600)2629978-1 21601836 nnns volume:10 year:2020 number:5 pages:1671-1683 https://doi.org/10.1534/g3.119.400884 kostenfrei https://doaj.org/article/5f190a0c498e44b98a8a018fd84b6993 kostenfrei http://g3journal.org/lookup/doi/10.1534/g3.119.400884 kostenfrei https://doaj.org/toc/2160-1836 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 5 1671-1683 |
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10.1534/g3.119.400884 doi (DE-627)DOAJ058418628 (DE-599)DOAJ5f190a0c498e44b98a8a018fd84b6993 DE-627 ger DE-627 rakwb eng QH426-470 Meng Lin verfasserin aut Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. cuticle cuticular conductance genome-wide association study rna sequencing whole-genome prediction Genetics Susanne Matschi verfasserin aut Miguel Vasquez verfasserin aut James Chamness verfasserin aut Nicholas Kaczmar verfasserin aut Matheus Baseggio verfasserin aut Michael Miller verfasserin aut Ethan L. Stewart verfasserin aut Pengfei Qiao verfasserin aut Michael J. Scanlon verfasserin aut Isabel Molina verfasserin aut Laurie G. Smith verfasserin aut Michael A. Gore verfasserin aut In G3: Genes, Genomes, Genetics Oxford University Press, 2012 10(2020), 5, Seite 1671-1683 (DE-627)668901071 (DE-600)2629978-1 21601836 nnns volume:10 year:2020 number:5 pages:1671-1683 https://doi.org/10.1534/g3.119.400884 kostenfrei https://doaj.org/article/5f190a0c498e44b98a8a018fd84b6993 kostenfrei http://g3journal.org/lookup/doi/10.1534/g3.119.400884 kostenfrei https://doaj.org/toc/2160-1836 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 5 1671-1683 |
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10.1534/g3.119.400884 doi (DE-627)DOAJ058418628 (DE-599)DOAJ5f190a0c498e44b98a8a018fd84b6993 DE-627 ger DE-627 rakwb eng QH426-470 Meng Lin verfasserin aut Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. cuticle cuticular conductance genome-wide association study rna sequencing whole-genome prediction Genetics Susanne Matschi verfasserin aut Miguel Vasquez verfasserin aut James Chamness verfasserin aut Nicholas Kaczmar verfasserin aut Matheus Baseggio verfasserin aut Michael Miller verfasserin aut Ethan L. Stewart verfasserin aut Pengfei Qiao verfasserin aut Michael J. Scanlon verfasserin aut Isabel Molina verfasserin aut Laurie G. Smith verfasserin aut Michael A. Gore verfasserin aut In G3: Genes, Genomes, Genetics Oxford University Press, 2012 10(2020), 5, Seite 1671-1683 (DE-627)668901071 (DE-600)2629978-1 21601836 nnns volume:10 year:2020 number:5 pages:1671-1683 https://doi.org/10.1534/g3.119.400884 kostenfrei https://doaj.org/article/5f190a0c498e44b98a8a018fd84b6993 kostenfrei http://g3journal.org/lookup/doi/10.1534/g3.119.400884 kostenfrei https://doaj.org/toc/2160-1836 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 5 1671-1683 |
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Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development |
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The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. |
| abstractGer |
The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. |
| abstract_unstemmed |
The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed at night and under water-limited conditions. Elucidating the genetic architecture of natural variation for leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we conducted a genome-wide association study of gc of adult leaves in a maize inbred association panel that was evaluated in four environments (Maricopa, AZ, and San Diego, CA, in 2016 and 2017). Five genomic regions significantly associated with gc were resolved to seven plausible candidate genes (ISTL1, two SEC14 homologs, cyclase-associated protein, a CER7 homolog, GDSL lipase, and β-D-XYLOSIDASE 4). These candidates are potentially involved in cuticle biosynthesis, trafficking and deposition of cuticle lipids, cutin polymerization, and cell wall modification. Laser microdissection RNA sequencing revealed that all these candidate genes, with the exception of the CER7 homolog, were expressed in the zone of the expanding adult maize leaf where cuticle maturation occurs. With direct application to genetic improvement, moderately high average predictive abilities were observed for whole-genome prediction of gc in locations (0.46 and 0.45) and across all environments (0.52). The findings of this study provide novel insights into the genetic control of gc and have the potential to help breeders more effectively develop drought-tolerant maize for target environments. |
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| title_short |
Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development |
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https://doi.org/10.1534/g3.119.400884 https://doaj.org/article/5f190a0c498e44b98a8a018fd84b6993 http://g3journal.org/lookup/doi/10.1534/g3.119.400884 https://doaj.org/toc/2160-1836 |
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| author2 |
Susanne Matschi Miguel Vasquez James Chamness Nicholas Kaczmar Matheus Baseggio Michael Miller Ethan L. Stewart Pengfei Qiao Michael J. Scanlon Isabel Molina Laurie G. Smith Michael A. Gore |
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Susanne Matschi Miguel Vasquez James Chamness Nicholas Kaczmar Matheus Baseggio Michael Miller Ethan L. Stewart Pengfei Qiao Michael J. Scanlon Isabel Molina Laurie G. Smith Michael A. Gore |
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10.1534/g3.119.400884 |
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| up_date |
2024-07-03T17:53:09.745Z |
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