Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells

The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-f...
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Autor*in:	Heng-Kien Au [verfasserIn] Syue-Wei Peng [verfasserIn] Chin-Lin Guo [verfasserIn] Chien-Chia Lin [verfasserIn] Yi-Lin Wang [verfasserIn] Yung-Che Kuo [verfasserIn] Tsz-Yau Law [verfasserIn] Hong-Nerng Ho [verfasserIn] Thai-Yen Ling [verfasserIn] Yen-Hua Huang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	germline stem cell niche extracellular matrix hypoxia laminin IGF

Übergeordnetes Werk:	In: Frontiers in Cell and Developmental Biology - Frontiers Media S.A., 2014, 9(2021)
Übergeordnetes Werk:	volume:9 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fcell.2021.646644

Katalog-ID:	DOAJ058529004

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allfields	10.3389/fcell.2021.646644 doi (DE-627)DOAJ058529004 (DE-599)DOAJb689affe981b4728ba7db464478e1c2e DE-627 ger DE-627 rakwb eng QH301-705.5 Heng-Kien Au verfasserin aut Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development. germline stem cell niche extracellular matrix hypoxia laminin IGF Biology (General) Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Syue-Wei Peng verfasserin aut Syue-Wei Peng verfasserin aut Chin-Lin Guo verfasserin aut Chien-Chia Lin verfasserin aut Chien-Chia Lin verfasserin aut Yi-Lin Wang verfasserin aut Yi-Lin Wang verfasserin aut Yung-Che Kuo verfasserin aut Yung-Che Kuo verfasserin aut Tsz-Yau Law verfasserin aut Tsz-Yau Law verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Thai-Yen Ling verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.646644 kostenfrei https://doaj.org/article/b689affe981b4728ba7db464478e1c2e kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.646644/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
spelling	10.3389/fcell.2021.646644 doi (DE-627)DOAJ058529004 (DE-599)DOAJb689affe981b4728ba7db464478e1c2e DE-627 ger DE-627 rakwb eng QH301-705.5 Heng-Kien Au verfasserin aut Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development. germline stem cell niche extracellular matrix hypoxia laminin IGF Biology (General) Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Syue-Wei Peng verfasserin aut Syue-Wei Peng verfasserin aut Chin-Lin Guo verfasserin aut Chien-Chia Lin verfasserin aut Chien-Chia Lin verfasserin aut Yi-Lin Wang verfasserin aut Yi-Lin Wang verfasserin aut Yung-Che Kuo verfasserin aut Yung-Che Kuo verfasserin aut Tsz-Yau Law verfasserin aut Tsz-Yau Law verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Thai-Yen Ling verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.646644 kostenfrei https://doaj.org/article/b689affe981b4728ba7db464478e1c2e kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.646644/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
allfields_unstemmed	10.3389/fcell.2021.646644 doi (DE-627)DOAJ058529004 (DE-599)DOAJb689affe981b4728ba7db464478e1c2e DE-627 ger DE-627 rakwb eng QH301-705.5 Heng-Kien Au verfasserin aut Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development. germline stem cell niche extracellular matrix hypoxia laminin IGF Biology (General) Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Syue-Wei Peng verfasserin aut Syue-Wei Peng verfasserin aut Chin-Lin Guo verfasserin aut Chien-Chia Lin verfasserin aut Chien-Chia Lin verfasserin aut Yi-Lin Wang verfasserin aut Yi-Lin Wang verfasserin aut Yung-Che Kuo verfasserin aut Yung-Che Kuo verfasserin aut Tsz-Yau Law verfasserin aut Tsz-Yau Law verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Thai-Yen Ling verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.646644 kostenfrei https://doaj.org/article/b689affe981b4728ba7db464478e1c2e kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.646644/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
allfieldsGer	10.3389/fcell.2021.646644 doi (DE-627)DOAJ058529004 (DE-599)DOAJb689affe981b4728ba7db464478e1c2e DE-627 ger DE-627 rakwb eng QH301-705.5 Heng-Kien Au verfasserin aut Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development. germline stem cell niche extracellular matrix hypoxia laminin IGF Biology (General) Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Syue-Wei Peng verfasserin aut Syue-Wei Peng verfasserin aut Chin-Lin Guo verfasserin aut Chien-Chia Lin verfasserin aut Chien-Chia Lin verfasserin aut Yi-Lin Wang verfasserin aut Yi-Lin Wang verfasserin aut Yung-Che Kuo verfasserin aut Yung-Che Kuo verfasserin aut Tsz-Yau Law verfasserin aut Tsz-Yau Law verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Thai-Yen Ling verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.646644 kostenfrei https://doaj.org/article/b689affe981b4728ba7db464478e1c2e kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.646644/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
allfieldsSound	10.3389/fcell.2021.646644 doi (DE-627)DOAJ058529004 (DE-599)DOAJb689affe981b4728ba7db464478e1c2e DE-627 ger DE-627 rakwb eng QH301-705.5 Heng-Kien Au verfasserin aut Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development. germline stem cell niche extracellular matrix hypoxia laminin IGF Biology (General) Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Heng-Kien Au verfasserin aut Syue-Wei Peng verfasserin aut Syue-Wei Peng verfasserin aut Chin-Lin Guo verfasserin aut Chien-Chia Lin verfasserin aut Chien-Chia Lin verfasserin aut Yi-Lin Wang verfasserin aut Yi-Lin Wang verfasserin aut Yung-Che Kuo verfasserin aut Yung-Che Kuo verfasserin aut Tsz-Yau Law verfasserin aut Tsz-Yau Law verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Hong-Nerng Ho verfasserin aut Thai-Yen Ling verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut Yen-Hua Huang verfasserin aut In Frontiers in Cell and Developmental Biology Frontiers Media S.A., 2014 9(2021) (DE-627)770398138 (DE-600)2737824-X 2296634X nnns volume:9 year:2021 https://doi.org/10.3389/fcell.2021.646644 kostenfrei https://doaj.org/article/b689affe981b4728ba7db464478e1c2e kostenfrei https://www.frontiersin.org/articles/10.3389/fcell.2021.646644/full kostenfrei https://doaj.org/toc/2296-634X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021
language	English
source	In Frontiers in Cell and Developmental Biology 9(2021) volume:9 year:2021
sourceStr	In Frontiers in Cell and Developmental Biology 9(2021) volume:9 year:2021
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	germline stem cell niche extracellular matrix hypoxia laminin IGF Biology (General)
isfreeaccess_bool	true
container_title	Frontiers in Cell and Developmental Biology
authorswithroles_txt_mv	Heng-Kien Au @@aut@@ Syue-Wei Peng @@aut@@ Chin-Lin Guo @@aut@@ Chien-Chia Lin @@aut@@ Yi-Lin Wang @@aut@@ Yung-Che Kuo @@aut@@ Tsz-Yau Law @@aut@@ Hong-Nerng Ho @@aut@@ Thai-Yen Ling @@aut@@ Yen-Hua Huang @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	770398138
id	DOAJ058529004
language_de	englisch
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callnumber-first	Q - Science
author	Heng-Kien Au
spellingShingle	Heng-Kien Au misc QH301-705.5 misc germline stem cell misc niche misc extracellular matrix misc hypoxia misc laminin misc IGF misc Biology (General) Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells
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topic_title	QH301-705.5 Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells germline stem cell niche extracellular matrix hypoxia laminin IGF
topic	misc QH301-705.5 misc germline stem cell misc niche misc extracellular matrix misc hypoxia misc laminin misc IGF misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc germline stem cell misc niche misc extracellular matrix misc hypoxia misc laminin misc IGF misc Biology (General)
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title	Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells
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title_full	Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells
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author_browse	Heng-Kien Au Syue-Wei Peng Chin-Lin Guo Chien-Chia Lin Yi-Lin Wang Yung-Che Kuo Tsz-Yau Law Hong-Nerng Ho Thai-Yen Ling Yen-Hua Huang
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title_sort	niche laminin and igf-1 additively coordinate the maintenance of oct-4 through cd49f/igf-1r-hif-2α feedforward loop in mouse germline stem cells
callnumber	QH301-705.5
title_auth	Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells
abstract	The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development.
abstractGer	The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development.
abstract_unstemmed	The mechanism on how extracellular matrix (ECM) cooperates with niche growth factors and oxygen tension to regulate the self-renewal of embryonic germline stem cells (GSCs) still remains unclear. Lacking of an appropriate in vitro cell model dramatically hinders the progress. Herein, using a serum-free culture system, we demonstrated that ECM laminin cooperated with hypoxia and insulin-like growth factor 1 receptor (IGF-1R) to additively maintain AP activity and Oct-4 expression of AP+GSCs. We found the laminin receptor CD49f expression in d2 testicular GSCs that were surrounded by laminin. Laminin and hypoxia significantly increased the GSC stemness-related genes, including Hif-2α, Oct-4, IGF-1R, and CD49f. Cotreatment of IGF-1 and laminin additively increased the expression of IGF-IR, CD49f, Hif-2α, and Oct-4. Conversely, silencing IGF-1R and/or CD49f decreased the expression of Hif-2α and Oct-4. The underlying mechanism involved CD49f/IGF1R-(PI3K/AKT)-Hif-2α signaling loop, which in turn maintains Oct-4 expression, symmetric self-renewal, and cell migration. These findings reveal the additive niche laminin/IGF-IR network during early GSC development.
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title_short	Niche Laminin and IGF-1 Additively Coordinate the Maintenance of Oct-4 Through CD49f/IGF-1R-Hif-2α Feedforward Loop in Mouse Germline Stem Cells
url	https://doi.org/10.3389/fcell.2021.646644 https://doaj.org/article/b689affe981b4728ba7db464478e1c2e https://www.frontiersin.org/articles/10.3389/fcell.2021.646644/full https://doaj.org/toc/2296-634X
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author2	Heng-Kien Au Syue-Wei Peng Chin-Lin Guo Chien-Chia Lin Yi-Lin Wang Yung-Che Kuo Tsz-Yau Law Hong-Nerng Ho Thai-Yen Ling Yen-Hua Huang
author2Str	Heng-Kien Au Syue-Wei Peng Chin-Lin Guo Chien-Chia Lin Yi-Lin Wang Yung-Che Kuo Tsz-Yau Law Hong-Nerng Ho Thai-Yen Ling Yen-Hua Huang
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up_date	2024-07-03T18:32:47.870Z
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