Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<

<p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Fossdal Carl Gunnar [verfasserIn] Yaqoob Nadeem [verfasserIn] Krokene Paal [verfasserIn] Kvaalen Harald [verfasserIn] Solheim Halvor [verfasserIn] Yakovlev Igor A [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	qRT–PCR Resistance Necrotroph MicroRNA

Übergeordnetes Werk:	In: BMC Plant Biology - BMC, 2003, 12(2012), 1, p 105
Übergeordnetes Werk:	volume:12 ; year:2012 ; number:1, p 105

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2229-12-105

Katalog-ID:	DOAJ05870695X

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245	1	0	\|a Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<
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520			\|a <p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p<
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700	0		\|a Yakovlev Igor A \|e verfasserin \|4 aut
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912			\|a GBV_ILN_4325
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author_variant	f c g fcg y n yn k p kp k h kh s h sh y i a yia
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allfields	10.1186/1471-2229-12-105 doi (DE-627)DOAJ05870695X (DE-599)DOAJ848e9c7f007e4189b8e0865e34040090 DE-627 ger DE-627 rakwb eng QK1-989 Fossdal Carl Gunnar verfasserin aut Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p< qRT–PCR Resistance <it<Ceratocystis polonica</it< Necrotroph <it<Picea abies</it< MicroRNA Botany Yaqoob Nadeem verfasserin aut Krokene Paal verfasserin aut Kvaalen Harald verfasserin aut Solheim Halvor verfasserin aut Yakovlev Igor A verfasserin aut In BMC Plant Biology BMC, 2003 12(2012), 1, p 105 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:12 year:2012 number:1, p 105 https://doi.org/10.1186/1471-2229-12-105 kostenfrei https://doaj.org/article/848e9c7f007e4189b8e0865e34040090 kostenfrei http://www.biomedcentral.com/1471-2229/12/105 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1, p 105
spelling	10.1186/1471-2229-12-105 doi (DE-627)DOAJ05870695X (DE-599)DOAJ848e9c7f007e4189b8e0865e34040090 DE-627 ger DE-627 rakwb eng QK1-989 Fossdal Carl Gunnar verfasserin aut Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p< qRT–PCR Resistance <it<Ceratocystis polonica</it< Necrotroph <it<Picea abies</it< MicroRNA Botany Yaqoob Nadeem verfasserin aut Krokene Paal verfasserin aut Kvaalen Harald verfasserin aut Solheim Halvor verfasserin aut Yakovlev Igor A verfasserin aut In BMC Plant Biology BMC, 2003 12(2012), 1, p 105 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:12 year:2012 number:1, p 105 https://doi.org/10.1186/1471-2229-12-105 kostenfrei https://doaj.org/article/848e9c7f007e4189b8e0865e34040090 kostenfrei http://www.biomedcentral.com/1471-2229/12/105 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1, p 105
allfields_unstemmed	10.1186/1471-2229-12-105 doi (DE-627)DOAJ05870695X (DE-599)DOAJ848e9c7f007e4189b8e0865e34040090 DE-627 ger DE-627 rakwb eng QK1-989 Fossdal Carl Gunnar verfasserin aut Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p< qRT–PCR Resistance <it<Ceratocystis polonica</it< Necrotroph <it<Picea abies</it< MicroRNA Botany Yaqoob Nadeem verfasserin aut Krokene Paal verfasserin aut Kvaalen Harald verfasserin aut Solheim Halvor verfasserin aut Yakovlev Igor A verfasserin aut In BMC Plant Biology BMC, 2003 12(2012), 1, p 105 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:12 year:2012 number:1, p 105 https://doi.org/10.1186/1471-2229-12-105 kostenfrei https://doaj.org/article/848e9c7f007e4189b8e0865e34040090 kostenfrei http://www.biomedcentral.com/1471-2229/12/105 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1, p 105
allfieldsGer	10.1186/1471-2229-12-105 doi (DE-627)DOAJ05870695X (DE-599)DOAJ848e9c7f007e4189b8e0865e34040090 DE-627 ger DE-627 rakwb eng QK1-989 Fossdal Carl Gunnar verfasserin aut Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p< qRT–PCR Resistance <it<Ceratocystis polonica</it< Necrotroph <it<Picea abies</it< MicroRNA Botany Yaqoob Nadeem verfasserin aut Krokene Paal verfasserin aut Kvaalen Harald verfasserin aut Solheim Halvor verfasserin aut Yakovlev Igor A verfasserin aut In BMC Plant Biology BMC, 2003 12(2012), 1, p 105 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:12 year:2012 number:1, p 105 https://doi.org/10.1186/1471-2229-12-105 kostenfrei https://doaj.org/article/848e9c7f007e4189b8e0865e34040090 kostenfrei http://www.biomedcentral.com/1471-2229/12/105 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1, p 105
allfieldsSound	10.1186/1471-2229-12-105 doi (DE-627)DOAJ05870695X (DE-599)DOAJ848e9c7f007e4189b8e0865e34040090 DE-627 ger DE-627 rakwb eng QK1-989 Fossdal Carl Gunnar verfasserin aut Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p< qRT–PCR Resistance <it<Ceratocystis polonica</it< Necrotroph <it<Picea abies</it< MicroRNA Botany Yaqoob Nadeem verfasserin aut Krokene Paal verfasserin aut Kvaalen Harald verfasserin aut Solheim Halvor verfasserin aut Yakovlev Igor A verfasserin aut In BMC Plant Biology BMC, 2003 12(2012), 1, p 105 (DE-627)335489060 (DE-600)2059868-3 14712229 nnns volume:12 year:2012 number:1, p 105 https://doi.org/10.1186/1471-2229-12-105 kostenfrei https://doaj.org/article/848e9c7f007e4189b8e0865e34040090 kostenfrei http://www.biomedcentral.com/1471-2229/12/105 kostenfrei https://doaj.org/toc/1471-2229 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2012 1, p 105
language	English
source	In BMC Plant Biology 12(2012), 1, p 105 volume:12 year:2012 number:1, p 105
sourceStr	In BMC Plant Biology 12(2012), 1, p 105 volume:12 year:2012 number:1, p 105
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	qRT–PCR Resistance <it<Ceratocystis polonica</it< Necrotroph <it<Picea abies</it< MicroRNA Botany
isfreeaccess_bool	true
container_title	BMC Plant Biology
authorswithroles_txt_mv	Fossdal Carl Gunnar @@aut@@ Yaqoob Nadeem @@aut@@ Krokene Paal @@aut@@ Kvaalen Harald @@aut@@ Solheim Halvor @@aut@@ Yakovlev Igor A @@aut@@
publishDateDaySort_date	2012-01-01T00:00:00Z
hierarchy_top_id	335489060
id	DOAJ05870695X
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ05870695X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230503005756.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2012 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1471-2229-12-105</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ05870695X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ848e9c7f007e4189b8e0865e34040090</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QK1-989</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Fossdal Carl Gunnar</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. 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callnumber-first	Q - Science
author	Fossdal Carl Gunnar
spellingShingle	Fossdal Carl Gunnar misc QK1-989 misc qRT–PCR misc Resistance misc <it<Ceratocystis polonica</it< misc Necrotroph misc <it<Picea abies</it< misc MicroRNA misc Botany Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<
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topic_title	QK1-989 Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it< qRT–PCR Resistance <it<Ceratocystis polonica</it< Necrotroph <it<Picea abies</it< MicroRNA
topic	misc QK1-989 misc qRT–PCR misc Resistance misc <it<Ceratocystis polonica</it< misc Necrotroph misc <it<Picea abies</it< misc MicroRNA misc Botany
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title	Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<
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title_full	Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<
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author_browse	Fossdal Carl Gunnar Yaqoob Nadeem Krokene Paal Kvaalen Harald Solheim Halvor Yakovlev Igor A
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title_sort	local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative micrornas in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<
callnumber	QK1-989
title_auth	Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<
abstract	<p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<NB-LRR resistance proteins are involved in recognizing pathogens and other exogenous stressors in plants. Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p<
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container_issue	1, p 105
title_short	Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<
url	https://doi.org/10.1186/1471-2229-12-105 https://doaj.org/article/848e9c7f007e4189b8e0865e34040090 http://www.biomedcentral.com/1471-2229/12/105 https://doaj.org/toc/1471-2229
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up_date	2024-07-03T19:37:28.291Z
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Resistance proteins are the first step in induced defence responses and a better understanding of their regulation is important to understand the mechanisms of plant defence. Much of the post-transcriptional regulation in plants is controlled by microRNAs (miRNA). We examined the expression of five Norway spruce miRNA that may regulate NB-LRR related transcripts in secondary phloem (bark) of resistant Norway spruce after wounding and inoculation with the necrotrophic blue stain fungus <it<Ceratocystis polonica</it<.</p< <p<Results</p< <p<The plants of this clone recovered from both the pathogen inoculations and wounding alone. We found local and systemic induction of the resistance marker genes <it<PaChi4, PaPAL</it< and <it<PaPX3</it< indicative of an effective induced host defence response. There were minor local and systemic changes in the expression of five miRNAs and 21 NB-LRRs between healthy and treated plants. Only five putative NB-LRRs (<it<PaLRR1, PaLRR3, PaLRR14, PaLRR15</it< and <it<PaLRR16</it<) showed significant increases greater than two-fold as a local response to <it<C. polonica</it<. Of all NB-LRRs only <it<PaLRR3</it<, the most highly differentially regulated NB-LRR, showed a significant increase also due to wounding. The five miRNAs showed indications of an initial local and systemic down-regulation at day 1, followed by a later increase up to and beyond the constitutive levels at day 6. However, the initial down-regulation was significant only for <it<miR3693</it< and <it<miR3705</it<.</p< <p<Conclusions</p< <p<Overall, local and systemic expression changes were evident only for the established resistance marker genes and <it<PaLRR3</it<. The minor expression changes observed both for the followed miRNAs and their predicted NB-LRR targets suggest that the expression of most NB-LRR genes are maintained close to their constitutive levels in stressed and healthy Norway spruce plants.</p<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">qRT–PCR</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Resistance</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><it<Ceratocystis polonica</it<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Necrotroph</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><it<Picea abies</it<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">MicroRNA</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Botany</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yaqoob 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Local and systemic changes in expression of resistance genes, <it<nb-lrr</it< genes and their putative microRNAs in norway spruce after wounding and inoculation with the pathogen <it<ceratocystis polonica</it<

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