High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it<

<p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Manabe Kenji [verfasserIn] Kageyama Yasushi [verfasserIn] Tohata Masatoshi [verfasserIn] Ara Katsutoshi [verfasserIn] Ozaki Katsuya [verfasserIn] Ogasawara Naotake [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2012

Schlagwörter:	α-Amylase AmyK38 CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response

Übergeordnetes Werk:	In: Microbial Cell Factories - BMC, 2003, 11(2012), 1, p 74
Übergeordnetes Werk:	volume:11 ; year:2012 ; number:1, p 74

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1475-2859-11-74

Katalog-ID:	DOAJ058715355

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allfields	10.1186/1475-2859-11-74 doi (DE-627)DOAJ058715355 (DE-599)DOAJ8de3857aabd5413ca732d64dac924b1c DE-627 ger DE-627 rakwb eng QR1-502 Manabe Kenji verfasserin aut High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p< α-Amylase AmyK38 <it<Bacillus subtilis</it< CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response Microbiology Kageyama Yasushi verfasserin aut Tohata Masatoshi verfasserin aut Ara Katsutoshi verfasserin aut Ozaki Katsuya verfasserin aut Ogasawara Naotake verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 74 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 74 https://doi.org/10.1186/1475-2859-11-74 kostenfrei https://doaj.org/article/8de3857aabd5413ca732d64dac924b1c kostenfrei http://www.microbialcellfactories.com/content/11/1/74 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 74
spelling	10.1186/1475-2859-11-74 doi (DE-627)DOAJ058715355 (DE-599)DOAJ8de3857aabd5413ca732d64dac924b1c DE-627 ger DE-627 rakwb eng QR1-502 Manabe Kenji verfasserin aut High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p< α-Amylase AmyK38 <it<Bacillus subtilis</it< CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response Microbiology Kageyama Yasushi verfasserin aut Tohata Masatoshi verfasserin aut Ara Katsutoshi verfasserin aut Ozaki Katsuya verfasserin aut Ogasawara Naotake verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 74 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 74 https://doi.org/10.1186/1475-2859-11-74 kostenfrei https://doaj.org/article/8de3857aabd5413ca732d64dac924b1c kostenfrei http://www.microbialcellfactories.com/content/11/1/74 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 74
allfields_unstemmed	10.1186/1475-2859-11-74 doi (DE-627)DOAJ058715355 (DE-599)DOAJ8de3857aabd5413ca732d64dac924b1c DE-627 ger DE-627 rakwb eng QR1-502 Manabe Kenji verfasserin aut High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p< α-Amylase AmyK38 <it<Bacillus subtilis</it< CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response Microbiology Kageyama Yasushi verfasserin aut Tohata Masatoshi verfasserin aut Ara Katsutoshi verfasserin aut Ozaki Katsuya verfasserin aut Ogasawara Naotake verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 74 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 74 https://doi.org/10.1186/1475-2859-11-74 kostenfrei https://doaj.org/article/8de3857aabd5413ca732d64dac924b1c kostenfrei http://www.microbialcellfactories.com/content/11/1/74 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 74
allfieldsGer	10.1186/1475-2859-11-74 doi (DE-627)DOAJ058715355 (DE-599)DOAJ8de3857aabd5413ca732d64dac924b1c DE-627 ger DE-627 rakwb eng QR1-502 Manabe Kenji verfasserin aut High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p< α-Amylase AmyK38 <it<Bacillus subtilis</it< CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response Microbiology Kageyama Yasushi verfasserin aut Tohata Masatoshi verfasserin aut Ara Katsutoshi verfasserin aut Ozaki Katsuya verfasserin aut Ogasawara Naotake verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 74 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 74 https://doi.org/10.1186/1475-2859-11-74 kostenfrei https://doaj.org/article/8de3857aabd5413ca732d64dac924b1c kostenfrei http://www.microbialcellfactories.com/content/11/1/74 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 74
allfieldsSound	10.1186/1475-2859-11-74 doi (DE-627)DOAJ058715355 (DE-599)DOAJ8de3857aabd5413ca732d64dac924b1c DE-627 ger DE-627 rakwb eng QR1-502 Manabe Kenji verfasserin aut High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it< 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p< α-Amylase AmyK38 <it<Bacillus subtilis</it< CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response Microbiology Kageyama Yasushi verfasserin aut Tohata Masatoshi verfasserin aut Ara Katsutoshi verfasserin aut Ozaki Katsuya verfasserin aut Ogasawara Naotake verfasserin aut In Microbial Cell Factories BMC, 2003 11(2012), 1, p 74 (DE-627)355987651 (DE-600)2091377-1 14752859 nnns volume:11 year:2012 number:1, p 74 https://doi.org/10.1186/1475-2859-11-74 kostenfrei https://doaj.org/article/8de3857aabd5413ca732d64dac924b1c kostenfrei http://www.microbialcellfactories.com/content/11/1/74 kostenfrei https://doaj.org/toc/1475-2859 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2012 1, p 74
language	English
source	In Microbial Cell Factories 11(2012), 1, p 74 volume:11 year:2012 number:1, p 74
sourceStr	In Microbial Cell Factories 11(2012), 1, p 74 volume:11 year:2012 number:1, p 74
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	α-Amylase AmyK38 <it<Bacillus subtilis</it< CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response Microbiology
isfreeaccess_bool	true
container_title	Microbial Cell Factories
authorswithroles_txt_mv	Manabe Kenji @@aut@@ Kageyama Yasushi @@aut@@ Tohata Masatoshi @@aut@@ Ara Katsutoshi @@aut@@ Ozaki Katsuya @@aut@@ Ogasawara Naotake @@aut@@
publishDateDaySort_date	2012-01-01T00:00:00Z
hierarchy_top_id	355987651
id	DOAJ058715355
language_de	englisch
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callnumber-first	Q - Science
author	Manabe Kenji
spellingShingle	Manabe Kenji misc QR1-502 misc α-Amylase misc AmyK38 misc <it<Bacillus subtilis</it< misc CssRS misc Genome reduction misc HtrA misc HtrB misc MGB874 misc Secretion stress response misc Microbiology High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it<
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topic_title	QR1-502 High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it< α-Amylase AmyK38 <it<Bacillus subtilis</it< CssRS Genome reduction HtrA HtrB MGB874 Secretion stress response
topic	misc QR1-502 misc α-Amylase misc AmyK38 misc <it<Bacillus subtilis</it< misc CssRS misc Genome reduction misc HtrA misc HtrB misc MGB874 misc Secretion stress response misc Microbiology
topic_unstemmed	misc QR1-502 misc α-Amylase misc AmyK38 misc <it<Bacillus subtilis</it< misc CssRS misc Genome reduction misc HtrA misc HtrB misc MGB874 misc Secretion stress response misc Microbiology
topic_browse	misc QR1-502 misc α-Amylase misc AmyK38 misc <it<Bacillus subtilis</it< misc CssRS misc Genome reduction misc HtrA misc HtrB misc MGB874 misc Secretion stress response misc Microbiology
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title	High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it<
ctrlnum	(DE-627)DOAJ058715355 (DE-599)DOAJ8de3857aabd5413ca732d64dac924b1c
title_full	High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it<
author_sort	Manabe Kenji
journal	Microbial Cell Factories
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author_browse	Manabe Kenji Kageyama Yasushi Tohata Masatoshi Ara Katsutoshi Ozaki Katsuya Ogasawara Naotake
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author-letter	Manabe Kenji
doi_str_mv	10.1186/1475-2859-11-74
author2-role	verfasserin
title_sort	high external ph enables more efficient secretion of alkaline α-amylase amyk38 by <it<bacillus subtilis</it<
callnumber	QR1-502
title_auth	High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it<
abstract	<p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p<
abstractGer	<p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p<
abstract_unstemmed	<p<<b<Abstract</b<</p< <p<<b<Background</b<</p< <p<<it<Bacillus subtilis</it< genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.</p< <p<<b<Results</b<</p< <p<Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of <it<rocG</it<, encoding glutamate dehydrogenase, whose activity leads to NH<sub<3</sub< production. Notably, in both the genome-reduced and wild-type strains, an up-shift of the external pH by the addition of an alkaline solution improved AmyK38 production, which was associated with alleviation of the secretion stress response. These results suggest that the optimal external pH for the secretion of AmyK38 is higher than the typical external pH of growth medium used to culture <it<B. subtilis</it<. Under controlled pH conditions, the highest production level (1.08 g l<sup<-1</sup<) of AmyK38 was obtained using strain MGB874.</p< <p<<b<Conclusions</b<</p< <p<We demonstrated for the first time that RocG is an important factor for secretory enzyme production in <it<B. subtilis</it< through its role in preventing acidification of the growth medium. As expected, a higher external pH enabled a more efficient secretion of the alkaline α-amylase AmyK38 in <it<B. subtilis</it<. Under controlled pH conditions, the reduced-genome strain MGB874 was demonstrated to be a beneficial host for the production of AmyK38.</p<
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title_short	High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it<
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High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by <it<Bacillus subtilis</it<

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