Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum

In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date...
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Gespeichert in:

Autor*in:	Hyun Tae Kim [verfasserIn] Enjian Jin [verfasserIn] Min-Ho Lee [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	lateral flow assay chemiluminescence detection portable platform cortisol human serum

Übergeordnetes Werk:	In: Biosensors - MDPI AG, 2012, 11(2021), 6, p 191
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:6, p 191

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/bios11060191

Katalog-ID:	DOAJ058794204

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520			\|a In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ.
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allfields	10.3390/bios11060191 doi (DE-627)DOAJ058794204 (DE-599)DOAJ8f4e7d0e33cd4f96805f47430582b979 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Hyun Tae Kim verfasserin aut Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. lateral flow assay chemiluminescence detection portable platform cortisol human serum Biotechnology Enjian Jin verfasserin aut Min-Ho Lee verfasserin aut In Biosensors MDPI AG, 2012 11(2021), 6, p 191 (DE-627)718626451 (DE-600)2662125-3 20796374 nnns volume:11 year:2021 number:6, p 191 https://doi.org/10.3390/bios11060191 kostenfrei https://doaj.org/article/8f4e7d0e33cd4f96805f47430582b979 kostenfrei https://www.mdpi.com/2079-6374/11/6/191 kostenfrei https://doaj.org/toc/2079-6374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 6, p 191
spelling	10.3390/bios11060191 doi (DE-627)DOAJ058794204 (DE-599)DOAJ8f4e7d0e33cd4f96805f47430582b979 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Hyun Tae Kim verfasserin aut Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. lateral flow assay chemiluminescence detection portable platform cortisol human serum Biotechnology Enjian Jin verfasserin aut Min-Ho Lee verfasserin aut In Biosensors MDPI AG, 2012 11(2021), 6, p 191 (DE-627)718626451 (DE-600)2662125-3 20796374 nnns volume:11 year:2021 number:6, p 191 https://doi.org/10.3390/bios11060191 kostenfrei https://doaj.org/article/8f4e7d0e33cd4f96805f47430582b979 kostenfrei https://www.mdpi.com/2079-6374/11/6/191 kostenfrei https://doaj.org/toc/2079-6374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 6, p 191
allfields_unstemmed	10.3390/bios11060191 doi (DE-627)DOAJ058794204 (DE-599)DOAJ8f4e7d0e33cd4f96805f47430582b979 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Hyun Tae Kim verfasserin aut Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. lateral flow assay chemiluminescence detection portable platform cortisol human serum Biotechnology Enjian Jin verfasserin aut Min-Ho Lee verfasserin aut In Biosensors MDPI AG, 2012 11(2021), 6, p 191 (DE-627)718626451 (DE-600)2662125-3 20796374 nnns volume:11 year:2021 number:6, p 191 https://doi.org/10.3390/bios11060191 kostenfrei https://doaj.org/article/8f4e7d0e33cd4f96805f47430582b979 kostenfrei https://www.mdpi.com/2079-6374/11/6/191 kostenfrei https://doaj.org/toc/2079-6374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 6, p 191
allfieldsGer	10.3390/bios11060191 doi (DE-627)DOAJ058794204 (DE-599)DOAJ8f4e7d0e33cd4f96805f47430582b979 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Hyun Tae Kim verfasserin aut Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. lateral flow assay chemiluminescence detection portable platform cortisol human serum Biotechnology Enjian Jin verfasserin aut Min-Ho Lee verfasserin aut In Biosensors MDPI AG, 2012 11(2021), 6, p 191 (DE-627)718626451 (DE-600)2662125-3 20796374 nnns volume:11 year:2021 number:6, p 191 https://doi.org/10.3390/bios11060191 kostenfrei https://doaj.org/article/8f4e7d0e33cd4f96805f47430582b979 kostenfrei https://www.mdpi.com/2079-6374/11/6/191 kostenfrei https://doaj.org/toc/2079-6374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 6, p 191
allfieldsSound	10.3390/bios11060191 doi (DE-627)DOAJ058794204 (DE-599)DOAJ8f4e7d0e33cd4f96805f47430582b979 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Hyun Tae Kim verfasserin aut Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ. lateral flow assay chemiluminescence detection portable platform cortisol human serum Biotechnology Enjian Jin verfasserin aut Min-Ho Lee verfasserin aut In Biosensors MDPI AG, 2012 11(2021), 6, p 191 (DE-627)718626451 (DE-600)2662125-3 20796374 nnns volume:11 year:2021 number:6, p 191 https://doi.org/10.3390/bios11060191 kostenfrei https://doaj.org/article/8f4e7d0e33cd4f96805f47430582b979 kostenfrei https://www.mdpi.com/2079-6374/11/6/191 kostenfrei https://doaj.org/toc/2079-6374 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 6, p 191
language	English
source	In Biosensors 11(2021), 6, p 191 volume:11 year:2021 number:6, p 191
sourceStr	In Biosensors 11(2021), 6, p 191 volume:11 year:2021 number:6, p 191
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	lateral flow assay chemiluminescence detection portable platform cortisol human serum Biotechnology
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container_title	Biosensors
authorswithroles_txt_mv	Hyun Tae Kim @@aut@@ Enjian Jin @@aut@@ Min-Ho Lee @@aut@@
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callnumber-first	T - Technology
author	Hyun Tae Kim
spellingShingle	Hyun Tae Kim misc TP248.13-248.65 misc lateral flow assay misc chemiluminescence detection misc portable platform misc cortisol misc human serum misc Biotechnology Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum
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topic_title	TP248.13-248.65 Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum lateral flow assay chemiluminescence detection portable platform cortisol human serum
topic	misc TP248.13-248.65 misc lateral flow assay misc chemiluminescence detection misc portable platform misc cortisol misc human serum misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc lateral flow assay misc chemiluminescence detection misc portable platform misc cortisol misc human serum misc Biotechnology
topic_browse	misc TP248.13-248.65 misc lateral flow assay misc chemiluminescence detection misc portable platform misc cortisol misc human serum misc Biotechnology
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title	Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum
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title_full	Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum
author_sort	Hyun Tae Kim
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author_browse	Hyun Tae Kim Enjian Jin Min-Ho Lee
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title_sort	portable chemiluminescence-based lateral flow assay platform for the detection of cortisol in human serum
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title_auth	Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum
abstract	In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ.
abstractGer	In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ.
abstract_unstemmed	In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R<sup<2</sup< = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R<sup<2</sup< = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ.
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container_issue	6, p 191
title_short	Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum
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up_date	2024-07-03T20:07:01.100Z
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