Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease
Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NER...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Adam Fabisiak [verfasserIn] Adrian Bartoszek [verfasserIn] Marcin Talar [verfasserIn] Agata Binienda [verfasserIn] Katarzyna Dziedziczak [verfasserIn] Julia B. Krajewska [verfasserIn] Paula Mosińska [verfasserIn] Karolina Niewinna [verfasserIn] Aleksandra Tarasiuk [verfasserIn] Anna Mokrowiecka [verfasserIn] Agnieszka Wierzchniewska-Ławska [verfasserIn] Ewa Małecka-Panas [verfasserIn] Maciej Salaga [verfasserIn] Jakub Fichna [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Journal of Clinical Medicine - MDPI AG, 2013, 9(2020), 12, p 4111 |
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| Übergeordnetes Werk: |
volume:9 ; year:2020 ; number:12, p 4111 |
| Links: |
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| DOI / URN: |
10.3390/jcm9124111 |
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| Katalog-ID: |
DOAJ05881552X |
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| 245 | 1 | 0 | |a Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease |
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| 520 | |a Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. | ||
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10.3390/jcm9124111 doi (DE-627)DOAJ05881552X (DE-599)DOAJ3a907339c5364bebb670ef97f9ba1a99 DE-627 ger DE-627 rakwb eng Adam Fabisiak verfasserin aut Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. free fatty acid receptor gastroesophageal reflux disease inflammation Medicine R Adrian Bartoszek verfasserin aut Marcin Talar verfasserin aut Agata Binienda verfasserin aut Katarzyna Dziedziczak verfasserin aut Julia B. Krajewska verfasserin aut Paula Mosińska verfasserin aut Karolina Niewinna verfasserin aut Aleksandra Tarasiuk verfasserin aut Anna Mokrowiecka verfasserin aut Agnieszka Wierzchniewska-Ławska verfasserin aut Ewa Małecka-Panas verfasserin aut Maciej Salaga verfasserin aut Jakub Fichna verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 9(2020), 12, p 4111 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:9 year:2020 number:12, p 4111 https://doi.org/10.3390/jcm9124111 kostenfrei https://doaj.org/article/3a907339c5364bebb670ef97f9ba1a99 kostenfrei https://www.mdpi.com/2077-0383/9/12/4111 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 12, p 4111 |
| spelling |
10.3390/jcm9124111 doi (DE-627)DOAJ05881552X (DE-599)DOAJ3a907339c5364bebb670ef97f9ba1a99 DE-627 ger DE-627 rakwb eng Adam Fabisiak verfasserin aut Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. free fatty acid receptor gastroesophageal reflux disease inflammation Medicine R Adrian Bartoszek verfasserin aut Marcin Talar verfasserin aut Agata Binienda verfasserin aut Katarzyna Dziedziczak verfasserin aut Julia B. Krajewska verfasserin aut Paula Mosińska verfasserin aut Karolina Niewinna verfasserin aut Aleksandra Tarasiuk verfasserin aut Anna Mokrowiecka verfasserin aut Agnieszka Wierzchniewska-Ławska verfasserin aut Ewa Małecka-Panas verfasserin aut Maciej Salaga verfasserin aut Jakub Fichna verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 9(2020), 12, p 4111 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:9 year:2020 number:12, p 4111 https://doi.org/10.3390/jcm9124111 kostenfrei https://doaj.org/article/3a907339c5364bebb670ef97f9ba1a99 kostenfrei https://www.mdpi.com/2077-0383/9/12/4111 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 12, p 4111 |
| allfields_unstemmed |
10.3390/jcm9124111 doi (DE-627)DOAJ05881552X (DE-599)DOAJ3a907339c5364bebb670ef97f9ba1a99 DE-627 ger DE-627 rakwb eng Adam Fabisiak verfasserin aut Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. free fatty acid receptor gastroesophageal reflux disease inflammation Medicine R Adrian Bartoszek verfasserin aut Marcin Talar verfasserin aut Agata Binienda verfasserin aut Katarzyna Dziedziczak verfasserin aut Julia B. Krajewska verfasserin aut Paula Mosińska verfasserin aut Karolina Niewinna verfasserin aut Aleksandra Tarasiuk verfasserin aut Anna Mokrowiecka verfasserin aut Agnieszka Wierzchniewska-Ławska verfasserin aut Ewa Małecka-Panas verfasserin aut Maciej Salaga verfasserin aut Jakub Fichna verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 9(2020), 12, p 4111 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:9 year:2020 number:12, p 4111 https://doi.org/10.3390/jcm9124111 kostenfrei https://doaj.org/article/3a907339c5364bebb670ef97f9ba1a99 kostenfrei https://www.mdpi.com/2077-0383/9/12/4111 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 12, p 4111 |
| allfieldsGer |
10.3390/jcm9124111 doi (DE-627)DOAJ05881552X (DE-599)DOAJ3a907339c5364bebb670ef97f9ba1a99 DE-627 ger DE-627 rakwb eng Adam Fabisiak verfasserin aut Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. free fatty acid receptor gastroesophageal reflux disease inflammation Medicine R Adrian Bartoszek verfasserin aut Marcin Talar verfasserin aut Agata Binienda verfasserin aut Katarzyna Dziedziczak verfasserin aut Julia B. Krajewska verfasserin aut Paula Mosińska verfasserin aut Karolina Niewinna verfasserin aut Aleksandra Tarasiuk verfasserin aut Anna Mokrowiecka verfasserin aut Agnieszka Wierzchniewska-Ławska verfasserin aut Ewa Małecka-Panas verfasserin aut Maciej Salaga verfasserin aut Jakub Fichna verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 9(2020), 12, p 4111 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:9 year:2020 number:12, p 4111 https://doi.org/10.3390/jcm9124111 kostenfrei https://doaj.org/article/3a907339c5364bebb670ef97f9ba1a99 kostenfrei https://www.mdpi.com/2077-0383/9/12/4111 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 12, p 4111 |
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10.3390/jcm9124111 doi (DE-627)DOAJ05881552X (DE-599)DOAJ3a907339c5364bebb670ef97f9ba1a99 DE-627 ger DE-627 rakwb eng Adam Fabisiak verfasserin aut Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. free fatty acid receptor gastroesophageal reflux disease inflammation Medicine R Adrian Bartoszek verfasserin aut Marcin Talar verfasserin aut Agata Binienda verfasserin aut Katarzyna Dziedziczak verfasserin aut Julia B. Krajewska verfasserin aut Paula Mosińska verfasserin aut Karolina Niewinna verfasserin aut Aleksandra Tarasiuk verfasserin aut Anna Mokrowiecka verfasserin aut Agnieszka Wierzchniewska-Ławska verfasserin aut Ewa Małecka-Panas verfasserin aut Maciej Salaga verfasserin aut Jakub Fichna verfasserin aut In Journal of Clinical Medicine MDPI AG, 2013 9(2020), 12, p 4111 (DE-627)718632478 (DE-600)2662592-1 20770383 nnns volume:9 year:2020 number:12, p 4111 https://doi.org/10.3390/jcm9124111 kostenfrei https://doaj.org/article/3a907339c5364bebb670ef97f9ba1a99 kostenfrei https://www.mdpi.com/2077-0383/9/12/4111 kostenfrei https://doaj.org/toc/2077-0383 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 12, p 4111 |
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Adam Fabisiak @@aut@@ Adrian Bartoszek @@aut@@ Marcin Talar @@aut@@ Agata Binienda @@aut@@ Katarzyna Dziedziczak @@aut@@ Julia B. Krajewska @@aut@@ Paula Mosińska @@aut@@ Karolina Niewinna @@aut@@ Aleksandra Tarasiuk @@aut@@ Anna Mokrowiecka @@aut@@ Agnieszka Wierzchniewska-Ławska @@aut@@ Ewa Małecka-Panas @@aut@@ Maciej Salaga @@aut@@ Jakub Fichna @@aut@@ |
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expression of ffar3 and ffar4 is increased in gastroesophageal reflux disease |
| title_auth |
Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease |
| abstract |
Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. |
| abstractGer |
Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. |
| abstract_unstemmed |
Background: The negative impact of a high-fat diet on the course of gastroesophageal reflux disease (GERD) has been previously reported. Free fatty acid receptors (FFARs) may be mediators of this phenomenon. The aim of this study was to characterize the role of FFARs in the course of nonerosive (NERD) and erosive (ERD) reflux disease. Methods: Collectively, 73 patients (62 with GERD and 11 healthy controls (HCs)) were recruited to the study. Esophageal biopsies were drawn from the lower third of the esophagus and kept for further experiments. Quantitative, real-time polymerase chain reaction was used to assess the expression of FFAR1, FFAR2, FFAR3, and FFAR4 in biopsies. Histological evaluation of dilated intracellular spaces (DISs) was also performed. Results: FFAR3 exhibited the highest expression, and FFAR4 exhibited the lowest expression in all esophageal samples. Higher relative expression of FFAR1 and FFAR2 and significantly higher expression of FFAR3 (<i<p</i< = 0.04) was noted in patients with GERD compared to respective HCs. Patients with nonerosive GERD (NERD) presented higher expression of all FFARs compared to patients with erosive GERD (ERD) and respective HCs. Interestingly, in patients with ERD, the expression of FFAR3 was lower than in HCs. Significant, weak, positive correlation was found for FFAR3 and FFAR4 expression and DIS scores (r = 0.36, <i<p</i< < 0.05 for FFAR 3, and r = 0.39, <i<p</i< < 0.05 for FFAR4). Conclusions: In this study, we show that FFARs may play a role in GERD pathogenesis, particularly in the NERD type. It may be assumed that FFARs, in particular FFAR3 and FFAR4, may have diagnostic and therapeutic potential in GERD. |
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| title_short |
Expression of FFAR3 and FFAR4 Is Increased in Gastroesophageal Reflux Disease |
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https://doi.org/10.3390/jcm9124111 https://doaj.org/article/3a907339c5364bebb670ef97f9ba1a99 https://www.mdpi.com/2077-0383/9/12/4111 https://doaj.org/toc/2077-0383 |
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Adrian Bartoszek Marcin Talar Agata Binienda Katarzyna Dziedziczak Julia B. Krajewska Paula Mosińska Karolina Niewinna Aleksandra Tarasiuk Anna Mokrowiecka Agnieszka Wierzchniewska-Ławska Ewa Małecka-Panas Maciej Salaga Jakub Fichna |
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Adrian Bartoszek Marcin Talar Agata Binienda Katarzyna Dziedziczak Julia B. Krajewska Paula Mosińska Karolina Niewinna Aleksandra Tarasiuk Anna Mokrowiecka Agnieszka Wierzchniewska-Ławska Ewa Małecka-Panas Maciej Salaga Jakub Fichna |
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