Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction

Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechani...
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Autor*in:	Isabel Witvrouwen [verfasserIn] Andreas B. Gevaert [verfasserIn] Nadine Possemiers [verfasserIn] Bert Ectors [verfasserIn] Tibor Stoop [verfasserIn] Inge Goovaerts [verfasserIn] Evi Boeren [verfasserIn] Wendy Hens [verfasserIn] Paul J. Beckers [verfasserIn] Anne Vorlat [verfasserIn] Hein Heidbuchel [verfasserIn] Amaryllis H. Van Craenenbroeck [verfasserIn] Emeline M. Van Craenenbroeck [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training

Übergeordnetes Werk:	In: Frontiers in Physiology - Frontiers Media S.A., 2011, 12(2021)
Übergeordnetes Werk:	volume:12 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fphys.2021.736494

Katalog-ID:	DOAJ058845682

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520			\|a Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement.
650		4	\|a microRNA
650		4	\|a HFrEF—heart failure with reduced ejection fraction
650		4	\|a VO2peak
650		4	\|a peak oxygen uptake
650		4	\|a response
650		4	\|a exercise training
653		0	\|a Physiology
700	0		\|a Isabel Witvrouwen \|e verfasserin \|4 aut
700	0		\|a Andreas B. Gevaert \|e verfasserin \|4 aut
700	0		\|a Andreas B. Gevaert \|e verfasserin \|4 aut
700	0		\|a Nadine Possemiers \|e verfasserin \|4 aut
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700	0		\|a Bert Ectors \|e verfasserin \|4 aut
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700	0		\|a Wendy Hens \|e verfasserin \|4 aut
700	0		\|a Wendy Hens \|e verfasserin \|4 aut
700	0		\|a Paul J. Beckers \|e verfasserin \|4 aut
700	0		\|a Paul J. Beckers \|e verfasserin \|4 aut
700	0		\|a Anne Vorlat \|e verfasserin \|4 aut
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allfields	10.3389/fphys.2021.736494 doi (DE-627)DOAJ058845682 (DE-599)DOAJc2eb0e6d968440b490d18ebc90943484 DE-627 ger DE-627 rakwb eng QP1-981 Isabel Witvrouwen verfasserin aut Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement. microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training Physiology Isabel Witvrouwen verfasserin aut Andreas B. Gevaert verfasserin aut Andreas B. Gevaert verfasserin aut Nadine Possemiers verfasserin aut Nadine Possemiers verfasserin aut Bert Ectors verfasserin aut Tibor Stoop verfasserin aut Inge Goovaerts verfasserin aut Evi Boeren verfasserin aut Wendy Hens verfasserin aut Wendy Hens verfasserin aut Paul J. Beckers verfasserin aut Paul J. Beckers verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut In Frontiers in Physiology Frontiers Media S.A., 2011 12(2021) (DE-627)631498788 (DE-600)2564217-0 1664042X nnns volume:12 year:2021 https://doi.org/10.3389/fphys.2021.736494 kostenfrei https://doaj.org/article/c2eb0e6d968440b490d18ebc90943484 kostenfrei https://www.frontiersin.org/articles/10.3389/fphys.2021.736494/full kostenfrei https://doaj.org/toc/1664-042X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
spelling	10.3389/fphys.2021.736494 doi (DE-627)DOAJ058845682 (DE-599)DOAJc2eb0e6d968440b490d18ebc90943484 DE-627 ger DE-627 rakwb eng QP1-981 Isabel Witvrouwen verfasserin aut Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement. microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training Physiology Isabel Witvrouwen verfasserin aut Andreas B. Gevaert verfasserin aut Andreas B. Gevaert verfasserin aut Nadine Possemiers verfasserin aut Nadine Possemiers verfasserin aut Bert Ectors verfasserin aut Tibor Stoop verfasserin aut Inge Goovaerts verfasserin aut Evi Boeren verfasserin aut Wendy Hens verfasserin aut Wendy Hens verfasserin aut Paul J. Beckers verfasserin aut Paul J. Beckers verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut In Frontiers in Physiology Frontiers Media S.A., 2011 12(2021) (DE-627)631498788 (DE-600)2564217-0 1664042X nnns volume:12 year:2021 https://doi.org/10.3389/fphys.2021.736494 kostenfrei https://doaj.org/article/c2eb0e6d968440b490d18ebc90943484 kostenfrei https://www.frontiersin.org/articles/10.3389/fphys.2021.736494/full kostenfrei https://doaj.org/toc/1664-042X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfields_unstemmed	10.3389/fphys.2021.736494 doi (DE-627)DOAJ058845682 (DE-599)DOAJc2eb0e6d968440b490d18ebc90943484 DE-627 ger DE-627 rakwb eng QP1-981 Isabel Witvrouwen verfasserin aut Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement. microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training Physiology Isabel Witvrouwen verfasserin aut Andreas B. Gevaert verfasserin aut Andreas B. Gevaert verfasserin aut Nadine Possemiers verfasserin aut Nadine Possemiers verfasserin aut Bert Ectors verfasserin aut Tibor Stoop verfasserin aut Inge Goovaerts verfasserin aut Evi Boeren verfasserin aut Wendy Hens verfasserin aut Wendy Hens verfasserin aut Paul J. Beckers verfasserin aut Paul J. Beckers verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut In Frontiers in Physiology Frontiers Media S.A., 2011 12(2021) (DE-627)631498788 (DE-600)2564217-0 1664042X nnns volume:12 year:2021 https://doi.org/10.3389/fphys.2021.736494 kostenfrei https://doaj.org/article/c2eb0e6d968440b490d18ebc90943484 kostenfrei https://www.frontiersin.org/articles/10.3389/fphys.2021.736494/full kostenfrei https://doaj.org/toc/1664-042X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsGer	10.3389/fphys.2021.736494 doi (DE-627)DOAJ058845682 (DE-599)DOAJc2eb0e6d968440b490d18ebc90943484 DE-627 ger DE-627 rakwb eng QP1-981 Isabel Witvrouwen verfasserin aut Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement. microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training Physiology Isabel Witvrouwen verfasserin aut Andreas B. Gevaert verfasserin aut Andreas B. Gevaert verfasserin aut Nadine Possemiers verfasserin aut Nadine Possemiers verfasserin aut Bert Ectors verfasserin aut Tibor Stoop verfasserin aut Inge Goovaerts verfasserin aut Evi Boeren verfasserin aut Wendy Hens verfasserin aut Wendy Hens verfasserin aut Paul J. Beckers verfasserin aut Paul J. Beckers verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut In Frontiers in Physiology Frontiers Media S.A., 2011 12(2021) (DE-627)631498788 (DE-600)2564217-0 1664042X nnns volume:12 year:2021 https://doi.org/10.3389/fphys.2021.736494 kostenfrei https://doaj.org/article/c2eb0e6d968440b490d18ebc90943484 kostenfrei https://www.frontiersin.org/articles/10.3389/fphys.2021.736494/full kostenfrei https://doaj.org/toc/1664-042X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsSound	10.3389/fphys.2021.736494 doi (DE-627)DOAJ058845682 (DE-599)DOAJc2eb0e6d968440b490d18ebc90943484 DE-627 ger DE-627 rakwb eng QP1-981 Isabel Witvrouwen verfasserin aut Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement. microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training Physiology Isabel Witvrouwen verfasserin aut Andreas B. Gevaert verfasserin aut Andreas B. Gevaert verfasserin aut Nadine Possemiers verfasserin aut Nadine Possemiers verfasserin aut Bert Ectors verfasserin aut Tibor Stoop verfasserin aut Inge Goovaerts verfasserin aut Evi Boeren verfasserin aut Wendy Hens verfasserin aut Wendy Hens verfasserin aut Paul J. Beckers verfasserin aut Paul J. Beckers verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Anne Vorlat verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Hein Heidbuchel verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Amaryllis H. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut Emeline M. Van Craenenbroeck verfasserin aut In Frontiers in Physiology Frontiers Media S.A., 2011 12(2021) (DE-627)631498788 (DE-600)2564217-0 1664042X nnns volume:12 year:2021 https://doi.org/10.3389/fphys.2021.736494 kostenfrei https://doaj.org/article/c2eb0e6d968440b490d18ebc90943484 kostenfrei https://www.frontiersin.org/articles/10.3389/fphys.2021.736494/full kostenfrei https://doaj.org/toc/1664-042X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
language	English
source	In Frontiers in Physiology 12(2021) volume:12 year:2021
sourceStr	In Frontiers in Physiology 12(2021) volume:12 year:2021
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training Physiology
isfreeaccess_bool	true
container_title	Frontiers in Physiology
authorswithroles_txt_mv	Isabel Witvrouwen @@aut@@ Andreas B. Gevaert @@aut@@ Nadine Possemiers @@aut@@ Bert Ectors @@aut@@ Tibor Stoop @@aut@@ Inge Goovaerts @@aut@@ Evi Boeren @@aut@@ Wendy Hens @@aut@@ Paul J. Beckers @@aut@@ Anne Vorlat @@aut@@ Hein Heidbuchel @@aut@@ Amaryllis H. Van Craenenbroeck @@aut@@ Emeline M. Van Craenenbroeck @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	631498788
id	DOAJ058845682
language_de	englisch
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We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction &lt; 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. 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callnumber-first	Q - Science
author	Isabel Witvrouwen
spellingShingle	Isabel Witvrouwen misc QP1-981 misc microRNA misc HFrEF—heart failure with reduced ejection fraction misc VO2peak misc peak oxygen uptake misc response misc exercise training misc Physiology Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction
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topic_title	QP1-981 Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction microRNA HFrEF—heart failure with reduced ejection fraction VO2peak peak oxygen uptake response exercise training
topic	misc QP1-981 misc microRNA misc HFrEF—heart failure with reduced ejection fraction misc VO2peak misc peak oxygen uptake misc response misc exercise training misc Physiology
topic_unstemmed	misc QP1-981 misc microRNA misc HFrEF—heart failure with reduced ejection fraction misc VO2peak misc peak oxygen uptake misc response misc exercise training misc Physiology
topic_browse	misc QP1-981 misc microRNA misc HFrEF—heart failure with reduced ejection fraction misc VO2peak misc peak oxygen uptake misc response misc exercise training misc Physiology
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title	Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction
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title_full	Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction
author_sort	Isabel Witvrouwen
journal	Frontiers in Physiology
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author_browse	Isabel Witvrouwen Andreas B. Gevaert Nadine Possemiers Bert Ectors Tibor Stoop Inge Goovaerts Evi Boeren Wendy Hens Paul J. Beckers Anne Vorlat Hein Heidbuchel Amaryllis H. Van Craenenbroeck Emeline M. Van Craenenbroeck
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title_sort	plasma-derived micrornas are influenced by acute and chronic exercise in patients with heart failure with reduced ejection fraction
callnumber	QP1-981
title_auth	Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction
abstract	Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement.
abstractGer	Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement.
abstract_unstemmed	Background: Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations.Methods: Twenty-five male HFrEF patients (left ventricular ejection fraction < 40%, New York Heart Association class ≥ II) participated in a 15-week combined strength and aerobic training program. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191, and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test (CPET), echocardiographic, vascular function, and muscle strength variables.Results: Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction (LVEF). Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index.Conclusion: Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in vitro studies on their molecular involvement.
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title_short	Plasma-Derived microRNAs Are Influenced by Acute and Chronic Exercise in Patients With Heart Failure With Reduced Ejection Fraction
url	https://doi.org/10.3389/fphys.2021.736494 https://doaj.org/article/c2eb0e6d968440b490d18ebc90943484 https://www.frontiersin.org/articles/10.3389/fphys.2021.736494/full https://doaj.org/toc/1664-042X
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author2	Isabel Witvrouwen Andreas B. Gevaert Nadine Possemiers Bert Ectors Tibor Stoop Inge Goovaerts Evi Boeren Wendy Hens Paul J. Beckers Anne Vorlat Hein Heidbuchel Amaryllis H. Van Craenenbroeck Emeline M. Van Craenenbroeck
author2Str	Isabel Witvrouwen Andreas B. Gevaert Nadine Possemiers Bert Ectors Tibor Stoop Inge Goovaerts Evi Boeren Wendy Hens Paul J. Beckers Anne Vorlat Hein Heidbuchel Amaryllis H. Van Craenenbroeck Emeline M. Van Craenenbroeck
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up_date	2024-07-03T20:23:52.461Z
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