Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice

Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins...
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Autor*in:	Long Zhang [verfasserIn] Yanzhou Qi [verfasserIn] Mingming Wu [verfasserIn] Lei Zhao [verfasserIn] Zhichao Zhao [verfasserIn] Cailin Lei [verfasserIn] Yuanyuan Hao [verfasserIn] Xiaowen Yu [verfasserIn] Yinglun Sun [verfasserIn] Xin Zhang [verfasserIn] Xiuping Guo [verfasserIn] Yulong Ren [verfasserIn] Jianmin Wan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Floury endosperm PPR Mitochondria RNA splicing Oryza sativa

Übergeordnetes Werk:	In: Crop Journal - KeAi Communications Co., Ltd., 2015, 9(2021), 2, Seite 282-296
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:2 ; pages:282-296

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.cj.2020.09.002

Katalog-ID:	DOAJ059331909

Internformat


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allfields	10.1016/j.cj.2020.09.002 doi (DE-627)DOAJ059331909 (DE-599)DOAJeb5ff70433984e40a7f8eb8291b62915 DE-627 ger DE-627 rakwb eng S1-972 Long Zhang verfasserin aut Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3. Floury endosperm PPR Mitochondria RNA splicing Oryza sativa Agriculture S Agriculture (General) Yanzhou Qi verfasserin aut Mingming Wu verfasserin aut Lei Zhao verfasserin aut Zhichao Zhao verfasserin aut Cailin Lei verfasserin aut Yuanyuan Hao verfasserin aut Xiaowen Yu verfasserin aut Yinglun Sun verfasserin aut Xin Zhang verfasserin aut Xiuping Guo verfasserin aut Yulong Ren verfasserin aut Jianmin Wan verfasserin aut In Crop Journal KeAi Communications Co., Ltd., 2015 9(2021), 2, Seite 282-296 (DE-627)774106883 (DE-600)2745450-2 22145141 nnns volume:9 year:2021 number:2 pages:282-296 https://doi.org/10.1016/j.cj.2020.09.002 kostenfrei https://doaj.org/article/eb5ff70433984e40a7f8eb8291b62915 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214514120301392 kostenfrei https://doaj.org/toc/2214-5141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 9 2021 2 282-296
spelling	10.1016/j.cj.2020.09.002 doi (DE-627)DOAJ059331909 (DE-599)DOAJeb5ff70433984e40a7f8eb8291b62915 DE-627 ger DE-627 rakwb eng S1-972 Long Zhang verfasserin aut Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3. Floury endosperm PPR Mitochondria RNA splicing Oryza sativa Agriculture S Agriculture (General) Yanzhou Qi verfasserin aut Mingming Wu verfasserin aut Lei Zhao verfasserin aut Zhichao Zhao verfasserin aut Cailin Lei verfasserin aut Yuanyuan Hao verfasserin aut Xiaowen Yu verfasserin aut Yinglun Sun verfasserin aut Xin Zhang verfasserin aut Xiuping Guo verfasserin aut Yulong Ren verfasserin aut Jianmin Wan verfasserin aut In Crop Journal KeAi Communications Co., Ltd., 2015 9(2021), 2, Seite 282-296 (DE-627)774106883 (DE-600)2745450-2 22145141 nnns volume:9 year:2021 number:2 pages:282-296 https://doi.org/10.1016/j.cj.2020.09.002 kostenfrei https://doaj.org/article/eb5ff70433984e40a7f8eb8291b62915 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214514120301392 kostenfrei https://doaj.org/toc/2214-5141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 9 2021 2 282-296
allfields_unstemmed	10.1016/j.cj.2020.09.002 doi (DE-627)DOAJ059331909 (DE-599)DOAJeb5ff70433984e40a7f8eb8291b62915 DE-627 ger DE-627 rakwb eng S1-972 Long Zhang verfasserin aut Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3. Floury endosperm PPR Mitochondria RNA splicing Oryza sativa Agriculture S Agriculture (General) Yanzhou Qi verfasserin aut Mingming Wu verfasserin aut Lei Zhao verfasserin aut Zhichao Zhao verfasserin aut Cailin Lei verfasserin aut Yuanyuan Hao verfasserin aut Xiaowen Yu verfasserin aut Yinglun Sun verfasserin aut Xin Zhang verfasserin aut Xiuping Guo verfasserin aut Yulong Ren verfasserin aut Jianmin Wan verfasserin aut In Crop Journal KeAi Communications Co., Ltd., 2015 9(2021), 2, Seite 282-296 (DE-627)774106883 (DE-600)2745450-2 22145141 nnns volume:9 year:2021 number:2 pages:282-296 https://doi.org/10.1016/j.cj.2020.09.002 kostenfrei https://doaj.org/article/eb5ff70433984e40a7f8eb8291b62915 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214514120301392 kostenfrei https://doaj.org/toc/2214-5141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 9 2021 2 282-296
allfieldsGer	10.1016/j.cj.2020.09.002 doi (DE-627)DOAJ059331909 (DE-599)DOAJeb5ff70433984e40a7f8eb8291b62915 DE-627 ger DE-627 rakwb eng S1-972 Long Zhang verfasserin aut Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3. Floury endosperm PPR Mitochondria RNA splicing Oryza sativa Agriculture S Agriculture (General) Yanzhou Qi verfasserin aut Mingming Wu verfasserin aut Lei Zhao verfasserin aut Zhichao Zhao verfasserin aut Cailin Lei verfasserin aut Yuanyuan Hao verfasserin aut Xiaowen Yu verfasserin aut Yinglun Sun verfasserin aut Xin Zhang verfasserin aut Xiuping Guo verfasserin aut Yulong Ren verfasserin aut Jianmin Wan verfasserin aut In Crop Journal KeAi Communications Co., Ltd., 2015 9(2021), 2, Seite 282-296 (DE-627)774106883 (DE-600)2745450-2 22145141 nnns volume:9 year:2021 number:2 pages:282-296 https://doi.org/10.1016/j.cj.2020.09.002 kostenfrei https://doaj.org/article/eb5ff70433984e40a7f8eb8291b62915 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214514120301392 kostenfrei https://doaj.org/toc/2214-5141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 9 2021 2 282-296
allfieldsSound	10.1016/j.cj.2020.09.002 doi (DE-627)DOAJ059331909 (DE-599)DOAJeb5ff70433984e40a7f8eb8291b62915 DE-627 ger DE-627 rakwb eng S1-972 Long Zhang verfasserin aut Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3. Floury endosperm PPR Mitochondria RNA splicing Oryza sativa Agriculture S Agriculture (General) Yanzhou Qi verfasserin aut Mingming Wu verfasserin aut Lei Zhao verfasserin aut Zhichao Zhao verfasserin aut Cailin Lei verfasserin aut Yuanyuan Hao verfasserin aut Xiaowen Yu verfasserin aut Yinglun Sun verfasserin aut Xin Zhang verfasserin aut Xiuping Guo verfasserin aut Yulong Ren verfasserin aut Jianmin Wan verfasserin aut In Crop Journal KeAi Communications Co., Ltd., 2015 9(2021), 2, Seite 282-296 (DE-627)774106883 (DE-600)2745450-2 22145141 nnns volume:9 year:2021 number:2 pages:282-296 https://doi.org/10.1016/j.cj.2020.09.002 kostenfrei https://doaj.org/article/eb5ff70433984e40a7f8eb8291b62915 kostenfrei http://www.sciencedirect.com/science/article/pii/S2214514120301392 kostenfrei https://doaj.org/toc/2214-5141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4700 GBV_ILN_4753 AR 9 2021 2 282-296
language	English
source	In Crop Journal 9(2021), 2, Seite 282-296 volume:9 year:2021 number:2 pages:282-296
sourceStr	In Crop Journal 9(2021), 2, Seite 282-296 volume:9 year:2021 number:2 pages:282-296
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Floury endosperm PPR Mitochondria RNA splicing Oryza sativa Agriculture S Agriculture (General)
isfreeaccess_bool	true
container_title	Crop Journal
authorswithroles_txt_mv	Long Zhang @@aut@@ Yanzhou Qi @@aut@@ Mingming Wu @@aut@@ Lei Zhao @@aut@@ Zhichao Zhao @@aut@@ Cailin Lei @@aut@@ Yuanyuan Hao @@aut@@ Xiaowen Yu @@aut@@ Yinglun Sun @@aut@@ Xin Zhang @@aut@@ Xiuping Guo @@aut@@ Yulong Ren @@aut@@ Jianmin Wan @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	774106883
id	DOAJ059331909
language_de	englisch
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Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. 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callnumber-first	S - Agriculture
author	Long Zhang
spellingShingle	Long Zhang misc S1-972 misc Floury endosperm misc PPR misc Mitochondria misc RNA splicing misc Oryza sativa misc Agriculture misc S misc Agriculture (General) Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice
authorStr	Long Zhang
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callnumber-label	S1-972
illustrated	Not Illustrated
issn	22145141
topic_title	S1-972 Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice Floury endosperm PPR Mitochondria RNA splicing Oryza sativa
topic	misc S1-972 misc Floury endosperm misc PPR misc Mitochondria misc RNA splicing misc Oryza sativa misc Agriculture misc S misc Agriculture (General)
topic_unstemmed	misc S1-972 misc Floury endosperm misc PPR misc Mitochondria misc RNA splicing misc Oryza sativa misc Agriculture misc S misc Agriculture (General)
topic_browse	misc S1-972 misc Floury endosperm misc PPR misc Mitochondria misc RNA splicing misc Oryza sativa misc Agriculture misc S misc Agriculture (General)
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice
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title_full	Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice
author_sort	Long Zhang
journal	Crop Journal
journalStr	Crop Journal
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author_browse	Long Zhang Yanzhou Qi Mingming Wu Lei Zhao Zhichao Zhao Cailin Lei Yuanyuan Hao Xiaowen Yu Yinglun Sun Xin Zhang Xiuping Guo Yulong Ren Jianmin Wan
container_volume	9
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format_se	Elektronische Aufsätze
author-letter	Long Zhang
doi_str_mv	10.1016/j.cj.2020.09.002
author2-role	verfasserin
title_sort	mitochondrion-targeted pentatricopeptide repeat5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice
callnumber	S1-972
title_auth	Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice
abstract	Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3.
abstractGer	Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3.
abstract_unstemmed	Endosperm as the storage organ of starch and protein in cereal crops largely determines grain yield and quality. Despite the fact that several pentatricopeptide repeat (PPR) proteins required for endosperm development have been identified in rice, the molecular mechanisms of many P-type PPR proteins in endosperm development remains unclear. Here, we isolated a rice floury endosperm mutant ppr5 that developed small starch grains and an abnormal aleurone layer, accompanied by decreased starch, protein, and amylose contents. Map-based cloning combined with a complementation test demonstrated that PPR5 encodes a P-type PPR protein that is localized to the mitochondria. The mutation in PPR5 caused reduced splicing efficiency of mitochondrial NADH dehydrogenase 4 (nad4) gene intron 3 and reduced complex I assembly and activity. Loss of PPR5 function greatly up-regulated expression of alternative oxidases (AOXs), reduced ATP production, and affected mitochondrial morphology. We demonstrate that PPR5, as a P-type PPR protein, is required for mitochondrial function and endosperm development by controlling the cis-splicing of mitochondrial nad4 intron 3.
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title_short	Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice
url	https://doi.org/10.1016/j.cj.2020.09.002 https://doaj.org/article/eb5ff70433984e40a7f8eb8291b62915 http://www.sciencedirect.com/science/article/pii/S2214514120301392 https://doaj.org/toc/2214-5141
remote_bool	true
author2	Yanzhou Qi Mingming Wu Lei Zhao Zhichao Zhao Cailin Lei Yuanyuan Hao Xiaowen Yu Yinglun Sun Xin Zhang Xiuping Guo Yulong Ren Jianmin Wan
author2Str	Yanzhou Qi Mingming Wu Lei Zhao Zhichao Zhao Cailin Lei Yuanyuan Hao Xiaowen Yu Yinglun Sun Xin Zhang Xiuping Guo Yulong Ren Jianmin Wan
ppnlink	774106883
callnumber-subject	S - General Agriculture
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.1016/j.cj.2020.09.002
callnumber-a	S1-972
up_date	2024-07-03T22:58:00.828Z
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Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice

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