Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay

As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Elisiane Coan Boian [verfasserIn] Renato Marques de Andrade [verfasserIn] Lílian Yurie Nakamura [verfasserIn] André Luiz Medeiros [verfasserIn] Marciele Coan Boian [verfasserIn] Sueli Donizete Borelli [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	2009

Schlagwörter:	antígenos solúveis HLA ELISA soluble antigens

Übergeordnetes Werk:	In: Acta Scientiarum. Health Sciences - Universidade Estadual de Maringá, 2008, 31(2009), 2, Seite 95-99
Übergeordnetes Werk:	volume:31 ; year:2009 ; number:2 ; pages:95-99

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ059612932

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allfields	(DE-627)DOAJ059612932 (DE-599)DOAJc0c43acf98f3461db100ed4eba9a255b DE-627 ger DE-627 rakwb eng por R5-920 RS1-441 Elisiane Coan Boian verfasserin aut Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation. antígenos solúveis HLA ELISA soluble antigens Medicine (General) Pharmacy and materia medica Renato Marques de Andrade verfasserin aut Lílian Yurie Nakamura verfasserin aut André Luiz Medeiros verfasserin aut Marciele Coan Boian verfasserin aut Sueli Donizete Borelli verfasserin aut In Acta Scientiarum. Health Sciences Universidade Estadual de Maringá, 2008 31(2009), 2, Seite 95-99 (DE-627)629833141 (DE-600)2558667-1 18078648 nnns volume:31 year:2009 number:2 pages:95-99 https://doaj.org/article/c0c43acf98f3461db100ed4eba9a255b kostenfrei http://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/6758/6758 kostenfrei https://doaj.org/toc/1679-9291 Journal toc kostenfrei https://doaj.org/toc/1807-8648 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2009 2 95-99
spelling	(DE-627)DOAJ059612932 (DE-599)DOAJc0c43acf98f3461db100ed4eba9a255b DE-627 ger DE-627 rakwb eng por R5-920 RS1-441 Elisiane Coan Boian verfasserin aut Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation. antígenos solúveis HLA ELISA soluble antigens Medicine (General) Pharmacy and materia medica Renato Marques de Andrade verfasserin aut Lílian Yurie Nakamura verfasserin aut André Luiz Medeiros verfasserin aut Marciele Coan Boian verfasserin aut Sueli Donizete Borelli verfasserin aut In Acta Scientiarum. Health Sciences Universidade Estadual de Maringá, 2008 31(2009), 2, Seite 95-99 (DE-627)629833141 (DE-600)2558667-1 18078648 nnns volume:31 year:2009 number:2 pages:95-99 https://doaj.org/article/c0c43acf98f3461db100ed4eba9a255b kostenfrei http://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/6758/6758 kostenfrei https://doaj.org/toc/1679-9291 Journal toc kostenfrei https://doaj.org/toc/1807-8648 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2009 2 95-99
allfields_unstemmed	(DE-627)DOAJ059612932 (DE-599)DOAJc0c43acf98f3461db100ed4eba9a255b DE-627 ger DE-627 rakwb eng por R5-920 RS1-441 Elisiane Coan Boian verfasserin aut Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation. antígenos solúveis HLA ELISA soluble antigens Medicine (General) Pharmacy and materia medica Renato Marques de Andrade verfasserin aut Lílian Yurie Nakamura verfasserin aut André Luiz Medeiros verfasserin aut Marciele Coan Boian verfasserin aut Sueli Donizete Borelli verfasserin aut In Acta Scientiarum. Health Sciences Universidade Estadual de Maringá, 2008 31(2009), 2, Seite 95-99 (DE-627)629833141 (DE-600)2558667-1 18078648 nnns volume:31 year:2009 number:2 pages:95-99 https://doaj.org/article/c0c43acf98f3461db100ed4eba9a255b kostenfrei http://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/6758/6758 kostenfrei https://doaj.org/toc/1679-9291 Journal toc kostenfrei https://doaj.org/toc/1807-8648 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2009 2 95-99
allfieldsGer	(DE-627)DOAJ059612932 (DE-599)DOAJc0c43acf98f3461db100ed4eba9a255b DE-627 ger DE-627 rakwb eng por R5-920 RS1-441 Elisiane Coan Boian verfasserin aut Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation. antígenos solúveis HLA ELISA soluble antigens Medicine (General) Pharmacy and materia medica Renato Marques de Andrade verfasserin aut Lílian Yurie Nakamura verfasserin aut André Luiz Medeiros verfasserin aut Marciele Coan Boian verfasserin aut Sueli Donizete Borelli verfasserin aut In Acta Scientiarum. Health Sciences Universidade Estadual de Maringá, 2008 31(2009), 2, Seite 95-99 (DE-627)629833141 (DE-600)2558667-1 18078648 nnns volume:31 year:2009 number:2 pages:95-99 https://doaj.org/article/c0c43acf98f3461db100ed4eba9a255b kostenfrei http://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/6758/6758 kostenfrei https://doaj.org/toc/1679-9291 Journal toc kostenfrei https://doaj.org/toc/1807-8648 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2009 2 95-99
allfieldsSound	(DE-627)DOAJ059612932 (DE-599)DOAJc0c43acf98f3461db100ed4eba9a255b DE-627 ger DE-627 rakwb eng por R5-920 RS1-441 Elisiane Coan Boian verfasserin aut Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation. antígenos solúveis HLA ELISA soluble antigens Medicine (General) Pharmacy and materia medica Renato Marques de Andrade verfasserin aut Lílian Yurie Nakamura verfasserin aut André Luiz Medeiros verfasserin aut Marciele Coan Boian verfasserin aut Sueli Donizete Borelli verfasserin aut In Acta Scientiarum. Health Sciences Universidade Estadual de Maringá, 2008 31(2009), 2, Seite 95-99 (DE-627)629833141 (DE-600)2558667-1 18078648 nnns volume:31 year:2009 number:2 pages:95-99 https://doaj.org/article/c0c43acf98f3461db100ed4eba9a255b kostenfrei http://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/6758/6758 kostenfrei https://doaj.org/toc/1679-9291 Journal toc kostenfrei https://doaj.org/toc/1807-8648 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2009 2 95-99
language	English Portuguese
source	In Acta Scientiarum. Health Sciences 31(2009), 2, Seite 95-99 volume:31 year:2009 number:2 pages:95-99
sourceStr	In Acta Scientiarum. Health Sciences 31(2009), 2, Seite 95-99 volume:31 year:2009 number:2 pages:95-99
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topic_facet	antígenos solúveis HLA ELISA soluble antigens Medicine (General) Pharmacy and materia medica
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authorswithroles_txt_mv	Elisiane Coan Boian @@aut@@ Renato Marques de Andrade @@aut@@ Lílian Yurie Nakamura @@aut@@ André Luiz Medeiros @@aut@@ Marciele Coan Boian @@aut@@ Sueli Donizete Borelli @@aut@@
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callnumber-first	R - Medicine
author	Elisiane Coan Boian
spellingShingle	Elisiane Coan Boian misc R5-920 misc RS1-441 misc antígenos solúveis misc HLA misc ELISA misc soluble antigens misc Medicine (General) misc Pharmacy and materia medica Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay
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topic_title	R5-920 RS1-441 Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay antígenos solúveis HLA ELISA soluble antigens
topic	misc R5-920 misc RS1-441 misc antígenos solúveis misc HLA misc ELISA misc soluble antigens misc Medicine (General) misc Pharmacy and materia medica
topic_unstemmed	misc R5-920 misc RS1-441 misc antígenos solúveis misc HLA misc ELISA misc soluble antigens misc Medicine (General) misc Pharmacy and materia medica
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title	Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay
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title_full	Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay
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author_browse	Elisiane Coan Boian Renato Marques de Andrade Lílian Yurie Nakamura André Luiz Medeiros Marciele Coan Boian Sueli Donizete Borelli
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title_sort	quantificação de antígenos hla classe i solúveis pela técnica de elisa = quantification of soluble hla class i antigens by elisa assay
callnumber	R5-920
title_auth	Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay
abstract	As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation.
abstractGer	As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation.
abstract_unstemmed	As moléculas HLA (Human Leucocyte Antigens) são consideradas,principalmente, estruturas de superfície celular envolvidas em uma variedade de reações imunes associadas com transplante, infecções e doenças autoimunes. Os antígenos HLA também podem ser encontrados, em forma solúvel, no soro e em diferentes fluidos do organismo humano. Este trabalho teve como objetivo desenvolver a técnica imunoenzimática (ELISA) para quantificar os níveis séricos de antígenos HLA classe I, específicos e totais, em indivíduos normais e em pacientes renais. A técnica de ELISA foi desenvolvida para demonstrar a presença, no soro, de antígenos HLA classe I totais (sHLAI) e as especificidades HLA-A2 (sHLA-A2) e HLA-B7 (sHLA-B7). Oitenta e oito amostras de soro foram envolvidas neste estudo, sendo 61 amostras provenientes de indivíduos sadios cadastrados no Hemocentro Regional de Maringá, Estado do Paraná, e 27 pacientes renais, provenientes dos centros de diálise da cidade de Maringá, Estado do Paraná. As concentrações médias de sHLA para as especificidades -A2 e -B7, detectadas somente em indivíduos sadios, foram 504.06 ng mL-1 ± 142.10 e 427.33 ng mL-1 ± 140.73, respectivamente. Resultados preliminares mostraram que sHLA-I, em indivíduos sadios, foi de 253,77 ng mL-1 e, em indivíduos renais em diálise, de 381,67 ng mL-1. A técnica de ELISA para detecção de antígenos HLA solúveis poderá ser útil em estudos comparativos, em diferentes populações saudáveis, diferentes patologias e no monitoramento das rejeições em transplantes.<br<<br<HLA (Human Leucocyte Antigens) molecules are regarded mainly as cell surface structures involved in several immune reactions associated with transplants, infections and auto-immune diseases. HLA antigens can be also found in soluble form in serum and in different fluids ofthe human body. The aim of this work was to develop the immunoenzymatic assay (ELISA) to quantify serum levels of specific and total soluble HLA class I antigens in normal individuals and in kidney patients. ELISA assay was developed to demonstrate the presence, in serum, of HLA-A2 (sHLA-A2), HLA-B7 (sHLA-B7) and total class I (sHLA-I) antigens. Eighty-eight serum samples were involved in this study, 61 from healthy individuals registered in the Regional Blood Center of the city of Maringá, Paraná State, and 27 kidney patients from dialysis centers of Maringá. The mean concentrations ofsHLA-A2 and sHLA-B7, in healthy individuals, were respectively, 504.06 ng mL-1 ± 142.10 and 427.33 ng mL-1 ± 140.73. Preliminary results showed that sHLA-I in healthy individuals was 253.77 ng mL-1, and 381.67 ng mL-1 in kidney dialysis patients. The ELISA assay couldbe useful to detect soluble HLA antigens in comparative studies in different healthy populations, in several pathologies and in monitoring rejection in transplantation.
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title_short	Quantificação de antígenos HLA classe I solúveis pela técnica de ELISA = Quantification of soluble HLA class I antigens by ELISA assay
url	https://doaj.org/article/c0c43acf98f3461db100ed4eba9a255b http://periodicos.uem.br/ojs/index.php/ActaSciHealthSci/article/view/6758/6758 https://doaj.org/toc/1679-9291 https://doaj.org/toc/1807-8648
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author2	Renato Marques de Andrade Lílian Yurie Nakamura André Luiz Medeiros Marciele Coan Boian Sueli Donizete Borelli
author2Str	Renato Marques de Andrade Lílian Yurie Nakamura André Luiz Medeiros Marciele Coan Boian Sueli Donizete Borelli
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up_date	2024-07-04T00:12:45.081Z
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