Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk
Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Samira Sangarifar [verfasserIn] Hassan Ghajavand [verfasserIn] Behrooz Johari [verfasserIn] Abazar Yari [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Anatomical Sciences Journal - Negah Institute for Scientific Communication, 2017, 14(2017), 2, Seite 91-96 |
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| Übergeordnetes Werk: |
volume:14 ; year:2017 ; number:2 ; pages:91-96 |
| Links: |
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DOAJ059758430 |
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| 520 | |a Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. | ||
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(DE-627)DOAJ059758430 (DE-599)DOAJ5888d891c3e54b5ead84aacbab359c9f DE-627 ger DE-627 rakwb eng QM1-695 Samira Sangarifar verfasserin aut Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. mrsa mec-a mec-c clf-a nosocomial infection cow’s milk Human anatomy Hassan Ghajavand verfasserin aut Behrooz Johari verfasserin aut Abazar Yari verfasserin aut In Anatomical Sciences Journal Negah Institute for Scientific Communication, 2017 14(2017), 2, Seite 91-96 (DE-627)1760616958 23223626 nnns volume:14 year:2017 number:2 pages:91-96 https://doaj.org/article/5888d891c3e54b5ead84aacbab359c9f kostenfrei http://anatomyjournal.ir/article-1-188-en.html kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 14 2017 2 91-96 |
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(DE-627)DOAJ059758430 (DE-599)DOAJ5888d891c3e54b5ead84aacbab359c9f DE-627 ger DE-627 rakwb eng QM1-695 Samira Sangarifar verfasserin aut Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. mrsa mec-a mec-c clf-a nosocomial infection cow’s milk Human anatomy Hassan Ghajavand verfasserin aut Behrooz Johari verfasserin aut Abazar Yari verfasserin aut In Anatomical Sciences Journal Negah Institute for Scientific Communication, 2017 14(2017), 2, Seite 91-96 (DE-627)1760616958 23223626 nnns volume:14 year:2017 number:2 pages:91-96 https://doaj.org/article/5888d891c3e54b5ead84aacbab359c9f kostenfrei http://anatomyjournal.ir/article-1-188-en.html kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 14 2017 2 91-96 |
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(DE-627)DOAJ059758430 (DE-599)DOAJ5888d891c3e54b5ead84aacbab359c9f DE-627 ger DE-627 rakwb eng QM1-695 Samira Sangarifar verfasserin aut Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. mrsa mec-a mec-c clf-a nosocomial infection cow’s milk Human anatomy Hassan Ghajavand verfasserin aut Behrooz Johari verfasserin aut Abazar Yari verfasserin aut In Anatomical Sciences Journal Negah Institute for Scientific Communication, 2017 14(2017), 2, Seite 91-96 (DE-627)1760616958 23223626 nnns volume:14 year:2017 number:2 pages:91-96 https://doaj.org/article/5888d891c3e54b5ead84aacbab359c9f kostenfrei http://anatomyjournal.ir/article-1-188-en.html kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 14 2017 2 91-96 |
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(DE-627)DOAJ059758430 (DE-599)DOAJ5888d891c3e54b5ead84aacbab359c9f DE-627 ger DE-627 rakwb eng QM1-695 Samira Sangarifar verfasserin aut Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. mrsa mec-a mec-c clf-a nosocomial infection cow’s milk Human anatomy Hassan Ghajavand verfasserin aut Behrooz Johari verfasserin aut Abazar Yari verfasserin aut In Anatomical Sciences Journal Negah Institute for Scientific Communication, 2017 14(2017), 2, Seite 91-96 (DE-627)1760616958 23223626 nnns volume:14 year:2017 number:2 pages:91-96 https://doaj.org/article/5888d891c3e54b5ead84aacbab359c9f kostenfrei http://anatomyjournal.ir/article-1-188-en.html kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 14 2017 2 91-96 |
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(DE-627)DOAJ059758430 (DE-599)DOAJ5888d891c3e54b5ead84aacbab359c9f DE-627 ger DE-627 rakwb eng QM1-695 Samira Sangarifar verfasserin aut Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. mrsa mec-a mec-c clf-a nosocomial infection cow’s milk Human anatomy Hassan Ghajavand verfasserin aut Behrooz Johari verfasserin aut Abazar Yari verfasserin aut In Anatomical Sciences Journal Negah Institute for Scientific Communication, 2017 14(2017), 2, Seite 91-96 (DE-627)1760616958 23223626 nnns volume:14 year:2017 number:2 pages:91-96 https://doaj.org/article/5888d891c3e54b5ead84aacbab359c9f kostenfrei http://anatomyjournal.ir/article-1-188-en.html kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei https://doaj.org/toc/2322-3626 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 14 2017 2 91-96 |
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Samira Sangarifar |
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QM1-695 Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk mrsa mec-a mec-c clf-a nosocomial infection cow’s milk |
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Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk |
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Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk |
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Samira Sangarifar Hassan Ghajavand Behrooz Johari Abazar Yari |
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frequency of clf-a, mec-a, and mec-c genes in staphylococcus aureus strains isolated from nosocomial infections and cow’s milk |
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QM1-695 |
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Frequency of clf-A, mec-A, and mec-C Genes in Staphylococcus Aureus Strains Isolated From Nosocomial Infections and Cow’s Milk |
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Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. |
| abstractGer |
Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. |
| abstract_unstemmed |
Introduction: There are various virulence factors encoded by Staphylococcus Aureus, which enable them to cause nosocomial infections and mastitis in dairy cattle. The clf-A gene mediates the bacterial colonization through binding to the extracellular matrix of the host. Treatment of such infections becomes more difficult due to increased resistance to methicillin. The current study aimed at investigating the frequency of clf-A, mec-A, and mec-C genes in S. Aureus strains isolated from hospital infections and cow’s milk. Methods: A total of 280 clinical samples as well as 100 milk samples from cattle with mastitis were collected. After identification of isolates, methicillin-resistant strains were identifies using agar screening and the cefoxitin disc diffusion test. In addition, the Minimum Inhibitory Concentration (MIC) value of oxacillin was determined using the E-test method. Polymerase Chain Reaction (PCR) was used to detect clf-A, mec-A, and mec-C genes in the methicillin-resistant isolates. Out of 120 S. Aureus strains isolated from nosocomial infections, 40 were identified as Methicillin-Resistant Staphylococcus Aureus (MRSA), while only 1 isolate from cow’s milk was MRSA. Results: Out of the 280 studied isolates, 35 clinical strains (87.5%) carried mec-A gene, and the frequency of clf-A gene was 80% and 100% in clinical and bovine milk samples, respectively. Importantly, MRSA strains harbouring clf-A gene, isolated from wound samples, exhibited the highest frequency. The mec-C gene was not found in clinical and milk isolated strains. The high frequency of clf-A gene in MRSA strains isolated from wound indicated a probable role of this virulence factor in skin colonization, as well as distribution and spread of the strains. Conclusion: Development of an appropriate method seems to be particularly useful for preventing the distribution of such strains. |
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https://doaj.org/article/5888d891c3e54b5ead84aacbab359c9f http://anatomyjournal.ir/article-1-188-en.html https://doaj.org/toc/2322-3626 |
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