Pulsed EPR Methods to Study Biomolecular Interactions

Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Irina Ritsch [verfasserIn] Daniel Klose [verfasserIn] Henrik Hintz [verfasserIn] Adelheid Godt [verfasserIn] Gunnar Jeschke [verfasserIn] Maxim Yulikov [verfasserIn]

Format:	E-Artikel
Sprache:	Deutsch ; Englisch ; Französisch

Erschienen:	2019

Schlagwörter:	Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling

Übergeordnetes Werk:	In: CHIMIA - Swiss Chemical Society, 2020, 73(2019), 4
Übergeordnetes Werk:	volume:73 ; year:2019 ; number:4

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.2533/chimia.2019.268

Katalog-ID:	DOAJ060057297

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ060057297
003	DE-627
005	20230502092042.0
007	cr uuu---uuuuu
008	230228s2019 xx \|\|\|\|\|o 00\| \|\|ger c
024	7		\|a 10.2533/chimia.2019.268 \|2 doi
035			\|a (DE-627)DOAJ060057297
035			\|a (DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a ger \|a eng \|a fre
050		0	\|a QD1-999
100	0		\|a Irina Ritsch \|e verfasserin \|4 aut
245	1	0	\|a Pulsed EPR Methods to Study Biomolecular Interactions
264		1	\|c 2019
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide.
650		4	\|a Deer
650		4	\|a Orthogonal spin labelling
650		4	\|a Pulsed dipolar spectroscopy
650		4	\|a Ridme
650		4	\|a Site-directed spin labelling
653		0	\|a Chemistry
700	0		\|a Daniel Klose \|e verfasserin \|4 aut
700	0		\|a Henrik Hintz \|e verfasserin \|4 aut
700	0		\|a Adelheid Godt \|e verfasserin \|4 aut
700	0		\|a Gunnar Jeschke \|e verfasserin \|4 aut
700	0		\|a Maxim Yulikov \|e verfasserin \|4 aut
773	0	8	\|i In \|t CHIMIA \|d Swiss Chemical Society, 2020 \|g 73(2019), 4 \|w (DE-627)480660301 \|w (DE-600)2179192-2 \|x 26732424 \|7 nnns
773	1	8	\|g volume:73 \|g year:2019 \|g number:4
856	4	0	\|u https://doi.org/10.2533/chimia.2019.268 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3 \|z kostenfrei
856	4	0	\|u https://www.chimia.ch/chimia/article/view/1226 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/0009-4293 \|y Journal toc \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/2673-2424 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 73 \|j 2019 \|e 4

Indexfelder

author_variant	i r ir d k dk h h hh a g ag g j gj m y my
matchkey_str	article:26732424:2019----::usdpmtosotdbooeu
hierarchy_sort_str	2019
callnumber-subject-code	QD
publishDate	2019
allfields	10.2533/chimia.2019.268 doi (DE-627)DOAJ060057297 (DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3 DE-627 ger DE-627 rakwb ger eng fre QD1-999 Irina Ritsch verfasserin aut Pulsed EPR Methods to Study Biomolecular Interactions 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide. Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling Chemistry Daniel Klose verfasserin aut Henrik Hintz verfasserin aut Adelheid Godt verfasserin aut Gunnar Jeschke verfasserin aut Maxim Yulikov verfasserin aut In CHIMIA Swiss Chemical Society, 2020 73(2019), 4 (DE-627)480660301 (DE-600)2179192-2 26732424 nnns volume:73 year:2019 number:4 https://doi.org/10.2533/chimia.2019.268 kostenfrei https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3 kostenfrei https://www.chimia.ch/chimia/article/view/1226 kostenfrei https://doaj.org/toc/0009-4293 Journal toc kostenfrei https://doaj.org/toc/2673-2424 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2019 4
spelling	10.2533/chimia.2019.268 doi (DE-627)DOAJ060057297 (DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3 DE-627 ger DE-627 rakwb ger eng fre QD1-999 Irina Ritsch verfasserin aut Pulsed EPR Methods to Study Biomolecular Interactions 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide. Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling Chemistry Daniel Klose verfasserin aut Henrik Hintz verfasserin aut Adelheid Godt verfasserin aut Gunnar Jeschke verfasserin aut Maxim Yulikov verfasserin aut In CHIMIA Swiss Chemical Society, 2020 73(2019), 4 (DE-627)480660301 (DE-600)2179192-2 26732424 nnns volume:73 year:2019 number:4 https://doi.org/10.2533/chimia.2019.268 kostenfrei https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3 kostenfrei https://www.chimia.ch/chimia/article/view/1226 kostenfrei https://doaj.org/toc/0009-4293 Journal toc kostenfrei https://doaj.org/toc/2673-2424 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2019 4
allfields_unstemmed	10.2533/chimia.2019.268 doi (DE-627)DOAJ060057297 (DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3 DE-627 ger DE-627 rakwb ger eng fre QD1-999 Irina Ritsch verfasserin aut Pulsed EPR Methods to Study Biomolecular Interactions 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide. Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling Chemistry Daniel Klose verfasserin aut Henrik Hintz verfasserin aut Adelheid Godt verfasserin aut Gunnar Jeschke verfasserin aut Maxim Yulikov verfasserin aut In CHIMIA Swiss Chemical Society, 2020 73(2019), 4 (DE-627)480660301 (DE-600)2179192-2 26732424 nnns volume:73 year:2019 number:4 https://doi.org/10.2533/chimia.2019.268 kostenfrei https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3 kostenfrei https://www.chimia.ch/chimia/article/view/1226 kostenfrei https://doaj.org/toc/0009-4293 Journal toc kostenfrei https://doaj.org/toc/2673-2424 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2019 4
allfieldsGer	10.2533/chimia.2019.268 doi (DE-627)DOAJ060057297 (DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3 DE-627 ger DE-627 rakwb ger eng fre QD1-999 Irina Ritsch verfasserin aut Pulsed EPR Methods to Study Biomolecular Interactions 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide. Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling Chemistry Daniel Klose verfasserin aut Henrik Hintz verfasserin aut Adelheid Godt verfasserin aut Gunnar Jeschke verfasserin aut Maxim Yulikov verfasserin aut In CHIMIA Swiss Chemical Society, 2020 73(2019), 4 (DE-627)480660301 (DE-600)2179192-2 26732424 nnns volume:73 year:2019 number:4 https://doi.org/10.2533/chimia.2019.268 kostenfrei https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3 kostenfrei https://www.chimia.ch/chimia/article/view/1226 kostenfrei https://doaj.org/toc/0009-4293 Journal toc kostenfrei https://doaj.org/toc/2673-2424 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2019 4
allfieldsSound	10.2533/chimia.2019.268 doi (DE-627)DOAJ060057297 (DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3 DE-627 ger DE-627 rakwb ger eng fre QD1-999 Irina Ritsch verfasserin aut Pulsed EPR Methods to Study Biomolecular Interactions 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide. Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling Chemistry Daniel Klose verfasserin aut Henrik Hintz verfasserin aut Adelheid Godt verfasserin aut Gunnar Jeschke verfasserin aut Maxim Yulikov verfasserin aut In CHIMIA Swiss Chemical Society, 2020 73(2019), 4 (DE-627)480660301 (DE-600)2179192-2 26732424 nnns volume:73 year:2019 number:4 https://doi.org/10.2533/chimia.2019.268 kostenfrei https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3 kostenfrei https://www.chimia.ch/chimia/article/view/1226 kostenfrei https://doaj.org/toc/0009-4293 Journal toc kostenfrei https://doaj.org/toc/2673-2424 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2019 4
language	German English French
source	In CHIMIA 73(2019), 4 volume:73 year:2019 number:4
sourceStr	In CHIMIA 73(2019), 4 volume:73 year:2019 number:4
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling Chemistry
isfreeaccess_bool	true
container_title	CHIMIA
authorswithroles_txt_mv	Irina Ritsch @@aut@@ Daniel Klose @@aut@@ Henrik Hintz @@aut@@ Adelheid Godt @@aut@@ Gunnar Jeschke @@aut@@ Maxim Yulikov @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	480660301
id	DOAJ060057297
language_de	deutsch englisch franzoesisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ060057297</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230502092042.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2019 xx \|\|\|\|\|o 00\| \|\|ger c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.2533/chimia.2019.268</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ060057297</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">ger</subfield><subfield code="a">eng</subfield><subfield code="a">fre</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QD1-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Irina Ritsch</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Pulsed EPR Methods to Study Biomolecular Interactions</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Deer</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Orthogonal spin labelling</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Pulsed dipolar spectroscopy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ridme</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Site-directed spin labelling</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemistry</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Daniel Klose</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Henrik Hintz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Adelheid Godt</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Gunnar Jeschke</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Maxim Yulikov</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">CHIMIA</subfield><subfield code="d">Swiss Chemical Society, 2020</subfield><subfield code="g">73(2019), 4</subfield><subfield code="w">(DE-627)480660301</subfield><subfield code="w">(DE-600)2179192-2</subfield><subfield code="x">26732424</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:73</subfield><subfield code="g">year:2019</subfield><subfield code="g">number:4</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.2533/chimia.2019.268</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.chimia.ch/chimia/article/view/1226</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/0009-4293</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2673-2424</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">73</subfield><subfield code="j">2019</subfield><subfield code="e">4</subfield></datafield></record></collection>
callnumber-first	Q - Science
author	Irina Ritsch
spellingShingle	Irina Ritsch misc QD1-999 misc Deer misc Orthogonal spin labelling misc Pulsed dipolar spectroscopy misc Ridme misc Site-directed spin labelling misc Chemistry Pulsed EPR Methods to Study Biomolecular Interactions
authorStr	Irina Ritsch
ppnlink_with_tag_str_mv	@@773@@(DE-627)480660301
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	QD1-999
illustrated	Not Illustrated
issn	26732424
topic_title	QD1-999 Pulsed EPR Methods to Study Biomolecular Interactions Deer Orthogonal spin labelling Pulsed dipolar spectroscopy Ridme Site-directed spin labelling
topic	misc QD1-999 misc Deer misc Orthogonal spin labelling misc Pulsed dipolar spectroscopy misc Ridme misc Site-directed spin labelling misc Chemistry
topic_unstemmed	misc QD1-999 misc Deer misc Orthogonal spin labelling misc Pulsed dipolar spectroscopy misc Ridme misc Site-directed spin labelling misc Chemistry
topic_browse	misc QD1-999 misc Deer misc Orthogonal spin labelling misc Pulsed dipolar spectroscopy misc Ridme misc Site-directed spin labelling misc Chemistry
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	CHIMIA
hierarchy_parent_id	480660301
hierarchy_top_title	CHIMIA
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)480660301 (DE-600)2179192-2
title	Pulsed EPR Methods to Study Biomolecular Interactions
ctrlnum	(DE-627)DOAJ060057297 (DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3
title_full	Pulsed EPR Methods to Study Biomolecular Interactions
author_sort	Irina Ritsch
journal	CHIMIA
journalStr	CHIMIA
callnumber-first-code	Q
lang_code	ger eng fre
isOA_bool	true
recordtype	marc
publishDateSort	2019
contenttype_str_mv	txt
author_browse	Irina Ritsch Daniel Klose Henrik Hintz Adelheid Godt Gunnar Jeschke Maxim Yulikov
container_volume	73
class	QD1-999
format_se	Elektronische Aufsätze
author-letter	Irina Ritsch
doi_str_mv	10.2533/chimia.2019.268
author2-role	verfasserin
title_sort	pulsed epr methods to study biomolecular interactions
callnumber	QD1-999
title_auth	Pulsed EPR Methods to Study Biomolecular Interactions
abstract	Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide.
abstractGer	Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide.
abstract_unstemmed	Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	4
title_short	Pulsed EPR Methods to Study Biomolecular Interactions
url	https://doi.org/10.2533/chimia.2019.268 https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3 https://www.chimia.ch/chimia/article/view/1226 https://doaj.org/toc/0009-4293 https://doaj.org/toc/2673-2424
remote_bool	true
author2	Daniel Klose Henrik Hintz Adelheid Godt Gunnar Jeschke Maxim Yulikov
author2Str	Daniel Klose Henrik Hintz Adelheid Godt Gunnar Jeschke Maxim Yulikov
ppnlink	480660301
callnumber-subject	QD - Chemistry
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.2533/chimia.2019.268
callnumber-a	QD1-999
up_date	2024-07-04T01:53:22.223Z
_version_	1803611539741081600
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ060057297</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230502092042.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2019 xx \|\|\|\|\|o 00\| \|\|ger c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.2533/chimia.2019.268</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ060057297</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ0f35d1a1914f4f54a57c621e9a738ca3</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">ger</subfield><subfield code="a">eng</subfield><subfield code="a">fre</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QD1-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Irina Ritsch</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Pulsed EPR Methods to Study Biomolecular Interactions</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Orthogonal site-directed spin labelling in combination with pulsed EPR spectroscopy is a powerful approach to study biomolecular interactions on a molecular level. Following a surge in pulse EPR method development, it is now possible to access distance distributions in the nanometre range in systems of complex composition. In this article we briefly outline the necessary considerations for measurements of distance distributions in macromolecular systems labelled with two or more different types of paramagnetic centres. We illustrate the approach with two examples: an application of the Double Electron-Electron Resonance (DEER) method on a triple spin-labelled protein dimer labelled with nitroxide and Gd(III), and an optimisation study of the Relaxation Induced Dipolar Modulation Enhancement (RIDME) experiment for the orthogonal spin pair Cu(II)-nitroxide.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Deer</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Orthogonal spin labelling</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Pulsed dipolar spectroscopy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ridme</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Site-directed spin labelling</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemistry</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Daniel Klose</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Henrik Hintz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Adelheid Godt</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Gunnar Jeschke</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Maxim Yulikov</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">CHIMIA</subfield><subfield code="d">Swiss Chemical Society, 2020</subfield><subfield code="g">73(2019), 4</subfield><subfield code="w">(DE-627)480660301</subfield><subfield code="w">(DE-600)2179192-2</subfield><subfield code="x">26732424</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:73</subfield><subfield code="g">year:2019</subfield><subfield code="g">number:4</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.2533/chimia.2019.268</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/0f35d1a1914f4f54a57c621e9a738ca3</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.chimia.ch/chimia/article/view/1226</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/0009-4293</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/2673-2424</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">73</subfield><subfield code="j">2019</subfield><subfield code="e">4</subfield></datafield></record></collection>
score	7.399684

Nicht das Richtige dabei?

Schreiben Sie uns!

Pulsed EPR Methods to Study Biomolecular Interactions

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?