Evolutionary diversification of SPANX-N sperm protein gene structure and expression.

The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the propo...
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Autor*in:	Natalay Kouprina [verfasserIn] Vladimir N Noskov [verfasserIn] Adam Pavlicek [verfasserIn] N Keith Collins [verfasserIn] Pamela D Schoppee Bortz [verfasserIn] Chris Ottolenghi [verfasserIn] Dmitri Loukinov [verfasserIn] Paul Goldsmith [verfasserIn] John I Risinger [verfasserIn] Jung-Hyun Kim [verfasserIn] V Anne Westbrook [verfasserIn] Gregory Solomon [verfasserIn] Hanna Sounders [verfasserIn] John C Herr [verfasserIn] Jerzy Jurka [verfasserIn] Victor Lobanenkov [verfasserIn] David Schlessinger [verfasserIn] Vladimir Larionov [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2007

Übergeordnetes Werk:	In: PLoS ONE - Public Library of Science (PLoS), 2007, 2(2007), 4, p e359
Übergeordnetes Werk:	volume:2 ; year:2007 ; number:4, p e359

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1371/journal.pone.0000359

Katalog-ID:	DOAJ060153539

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100	0		\|a Natalay Kouprina \|e verfasserin \|4 aut
245	1	0	\|a Evolutionary diversification of SPANX-N sperm protein gene structure and expression.
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520			\|a The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well.
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700	0		\|a Adam Pavlicek \|e verfasserin \|4 aut
700	0		\|a N Keith Collins \|e verfasserin \|4 aut
700	0		\|a Pamela D Schoppee Bortz \|e verfasserin \|4 aut
700	0		\|a Chris Ottolenghi \|e verfasserin \|4 aut
700	0		\|a Dmitri Loukinov \|e verfasserin \|4 aut
700	0		\|a Paul Goldsmith \|e verfasserin \|4 aut
700	0		\|a John I Risinger \|e verfasserin \|4 aut
700	0		\|a Jung-Hyun Kim \|e verfasserin \|4 aut
700	0		\|a V Anne Westbrook \|e verfasserin \|4 aut
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700	0		\|a Victor Lobanenkov \|e verfasserin \|4 aut
700	0		\|a David Schlessinger \|e verfasserin \|4 aut
700	0		\|a Vladimir Larionov \|e verfasserin \|4 aut
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912			\|a GBV_ILN_4323
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author_variant	n k nk v n n vnn a p ap n k c nkc p d s b pdsb c o co d l dl p g pg j i r jir j h k jhk v a w vaw g s gs h s hs j c h jch j j jj v l vl d s ds v l vl
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publishDate	2007
allfields	10.1371/journal.pone.0000359 doi (DE-627)DOAJ060153539 (DE-599)DOAJeb55759c6a8b47629bffb4a26a4f7f80 DE-627 ger DE-627 rakwb eng Natalay Kouprina verfasserin aut Evolutionary diversification of SPANX-N sperm protein gene structure and expression. 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well. Medicine R Science Q Vladimir N Noskov verfasserin aut Adam Pavlicek verfasserin aut N Keith Collins verfasserin aut Pamela D Schoppee Bortz verfasserin aut Chris Ottolenghi verfasserin aut Dmitri Loukinov verfasserin aut Paul Goldsmith verfasserin aut John I Risinger verfasserin aut Jung-Hyun Kim verfasserin aut V Anne Westbrook verfasserin aut Gregory Solomon verfasserin aut Hanna Sounders verfasserin aut John C Herr verfasserin aut Jerzy Jurka verfasserin aut Victor Lobanenkov verfasserin aut David Schlessinger verfasserin aut Vladimir Larionov verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 2(2007), 4, p e359 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:2 year:2007 number:4, p e359 https://doi.org/10.1371/journal.pone.0000359 kostenfrei https://doaj.org/article/eb55759c6a8b47629bffb4a26a4f7f80 kostenfrei http://europepmc.org/articles/PMC1831492?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4, p e359
spelling	10.1371/journal.pone.0000359 doi (DE-627)DOAJ060153539 (DE-599)DOAJeb55759c6a8b47629bffb4a26a4f7f80 DE-627 ger DE-627 rakwb eng Natalay Kouprina verfasserin aut Evolutionary diversification of SPANX-N sperm protein gene structure and expression. 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well. Medicine R Science Q Vladimir N Noskov verfasserin aut Adam Pavlicek verfasserin aut N Keith Collins verfasserin aut Pamela D Schoppee Bortz verfasserin aut Chris Ottolenghi verfasserin aut Dmitri Loukinov verfasserin aut Paul Goldsmith verfasserin aut John I Risinger verfasserin aut Jung-Hyun Kim verfasserin aut V Anne Westbrook verfasserin aut Gregory Solomon verfasserin aut Hanna Sounders verfasserin aut John C Herr verfasserin aut Jerzy Jurka verfasserin aut Victor Lobanenkov verfasserin aut David Schlessinger verfasserin aut Vladimir Larionov verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 2(2007), 4, p e359 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:2 year:2007 number:4, p e359 https://doi.org/10.1371/journal.pone.0000359 kostenfrei https://doaj.org/article/eb55759c6a8b47629bffb4a26a4f7f80 kostenfrei http://europepmc.org/articles/PMC1831492?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4, p e359
allfields_unstemmed	10.1371/journal.pone.0000359 doi (DE-627)DOAJ060153539 (DE-599)DOAJeb55759c6a8b47629bffb4a26a4f7f80 DE-627 ger DE-627 rakwb eng Natalay Kouprina verfasserin aut Evolutionary diversification of SPANX-N sperm protein gene structure and expression. 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well. Medicine R Science Q Vladimir N Noskov verfasserin aut Adam Pavlicek verfasserin aut N Keith Collins verfasserin aut Pamela D Schoppee Bortz verfasserin aut Chris Ottolenghi verfasserin aut Dmitri Loukinov verfasserin aut Paul Goldsmith verfasserin aut John I Risinger verfasserin aut Jung-Hyun Kim verfasserin aut V Anne Westbrook verfasserin aut Gregory Solomon verfasserin aut Hanna Sounders verfasserin aut John C Herr verfasserin aut Jerzy Jurka verfasserin aut Victor Lobanenkov verfasserin aut David Schlessinger verfasserin aut Vladimir Larionov verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 2(2007), 4, p e359 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:2 year:2007 number:4, p e359 https://doi.org/10.1371/journal.pone.0000359 kostenfrei https://doaj.org/article/eb55759c6a8b47629bffb4a26a4f7f80 kostenfrei http://europepmc.org/articles/PMC1831492?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4, p e359
allfieldsGer	10.1371/journal.pone.0000359 doi (DE-627)DOAJ060153539 (DE-599)DOAJeb55759c6a8b47629bffb4a26a4f7f80 DE-627 ger DE-627 rakwb eng Natalay Kouprina verfasserin aut Evolutionary diversification of SPANX-N sperm protein gene structure and expression. 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well. Medicine R Science Q Vladimir N Noskov verfasserin aut Adam Pavlicek verfasserin aut N Keith Collins verfasserin aut Pamela D Schoppee Bortz verfasserin aut Chris Ottolenghi verfasserin aut Dmitri Loukinov verfasserin aut Paul Goldsmith verfasserin aut John I Risinger verfasserin aut Jung-Hyun Kim verfasserin aut V Anne Westbrook verfasserin aut Gregory Solomon verfasserin aut Hanna Sounders verfasserin aut John C Herr verfasserin aut Jerzy Jurka verfasserin aut Victor Lobanenkov verfasserin aut David Schlessinger verfasserin aut Vladimir Larionov verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 2(2007), 4, p e359 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:2 year:2007 number:4, p e359 https://doi.org/10.1371/journal.pone.0000359 kostenfrei https://doaj.org/article/eb55759c6a8b47629bffb4a26a4f7f80 kostenfrei http://europepmc.org/articles/PMC1831492?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4, p e359
allfieldsSound	10.1371/journal.pone.0000359 doi (DE-627)DOAJ060153539 (DE-599)DOAJeb55759c6a8b47629bffb4a26a4f7f80 DE-627 ger DE-627 rakwb eng Natalay Kouprina verfasserin aut Evolutionary diversification of SPANX-N sperm protein gene structure and expression. 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well. Medicine R Science Q Vladimir N Noskov verfasserin aut Adam Pavlicek verfasserin aut N Keith Collins verfasserin aut Pamela D Schoppee Bortz verfasserin aut Chris Ottolenghi verfasserin aut Dmitri Loukinov verfasserin aut Paul Goldsmith verfasserin aut John I Risinger verfasserin aut Jung-Hyun Kim verfasserin aut V Anne Westbrook verfasserin aut Gregory Solomon verfasserin aut Hanna Sounders verfasserin aut John C Herr verfasserin aut Jerzy Jurka verfasserin aut Victor Lobanenkov verfasserin aut David Schlessinger verfasserin aut Vladimir Larionov verfasserin aut In PLoS ONE Public Library of Science (PLoS), 2007 2(2007), 4, p e359 (DE-627)523574592 (DE-600)2267670-3 19326203 nnns volume:2 year:2007 number:4, p e359 https://doi.org/10.1371/journal.pone.0000359 kostenfrei https://doaj.org/article/eb55759c6a8b47629bffb4a26a4f7f80 kostenfrei http://europepmc.org/articles/PMC1831492?pdf=render kostenfrei https://doaj.org/toc/1932-6203 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_34 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_235 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2007 4, p e359
language	English
source	In PLoS ONE 2(2007), 4, p e359 volume:2 year:2007 number:4, p e359
sourceStr	In PLoS ONE 2(2007), 4, p e359 volume:2 year:2007 number:4, p e359
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Medicine R Science Q
isfreeaccess_bool	true
container_title	PLoS ONE
authorswithroles_txt_mv	Natalay Kouprina @@aut@@ Vladimir N Noskov @@aut@@ Adam Pavlicek @@aut@@ N Keith Collins @@aut@@ Pamela D Schoppee Bortz @@aut@@ Chris Ottolenghi @@aut@@ Dmitri Loukinov @@aut@@ Paul Goldsmith @@aut@@ John I Risinger @@aut@@ Jung-Hyun Kim @@aut@@ V Anne Westbrook @@aut@@ Gregory Solomon @@aut@@ Hanna Sounders @@aut@@ John C Herr @@aut@@ Jerzy Jurka @@aut@@ Victor Lobanenkov @@aut@@ David Schlessinger @@aut@@ Vladimir Larionov @@aut@@
publishDateDaySort_date	2007-01-01T00:00:00Z
hierarchy_top_id	523574592
id	DOAJ060153539
language_de	englisch
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author	Natalay Kouprina
spellingShingle	Natalay Kouprina misc Medicine misc R misc Science misc Q Evolutionary diversification of SPANX-N sperm protein gene structure and expression.
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topic_title	Evolutionary diversification of SPANX-N sperm protein gene structure and expression
topic	misc Medicine misc R misc Science misc Q
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title	Evolutionary diversification of SPANX-N sperm protein gene structure and expression.
ctrlnum	(DE-627)DOAJ060153539 (DE-599)DOAJeb55759c6a8b47629bffb4a26a4f7f80
title_full	Evolutionary diversification of SPANX-N sperm protein gene structure and expression
author_sort	Natalay Kouprina
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author_browse	Natalay Kouprina Vladimir N Noskov Adam Pavlicek N Keith Collins Pamela D Schoppee Bortz Chris Ottolenghi Dmitri Loukinov Paul Goldsmith John I Risinger Jung-Hyun Kim V Anne Westbrook Gregory Solomon Hanna Sounders John C Herr Jerzy Jurka Victor Lobanenkov David Schlessinger Vladimir Larionov
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author-letter	Natalay Kouprina
doi_str_mv	10.1371/journal.pone.0000359
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title_sort	evolutionary diversification of spanx-n sperm protein gene structure and expression
title_auth	Evolutionary diversification of SPANX-N sperm protein gene structure and expression.
abstract	The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well.
abstractGer	The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well.
abstract_unstemmed	The sperm protein associated with nucleus in the X chromosome (SPANX) genes cluster at Xq27 in two subfamilies, SPANX-A/D and SPANX-N. SPANX-A/D is specific for hominoids and is fairly well characterized. The SPANX-N gave rise to SPANX-A/D in the hominoid lineage approximately 7 MYA. Given the proposed role of SPANX genes in spermatogenesis, we have extended studies to SPANX-N gene evolution, variation, regulation of expression, and intra-sperm localization. By immunofluorescence analysis, SPANX-N proteins are localized in post-meiotic spermatids exclusively, like SPANX-A/D. But in contrast to SPANX-A/D, SPANX-N are found in all ejaculated spermatozoa rather than only in a subpopulation, are localized in the acrosome rather than in the nuclear envelope, and are expressed at a low level in several nongametogenic adult tissues as well as many cancers. Presence of a binding site for CTCF and its testis-specific paralogue BORIS in the SPANX promoters suggests, by analogy to MAGE-A1 and NY-ESO-1, that their activation in spermatogenesis is mediated by the programmed replacement of CTCF by BORIS. Based on the relative density of CpG, the more extended expression of SPANX-N compared to SPANX-A/D in nongametogenic tissues is likely attributed to differences in promoter methylation. Our findings suggest that the recent duplication of SPANX genes in hominoids was accompanied by different localization of SPANX-N proteins in post-meiotic sperm and additional expression in several nongonadal tissues. This suggests a corresponding functional diversification of SPANX gene families in hominoids. SPANX proteins thus provide unique targets to investigate their roles in the function of spermatozoa, selected malignancies, and for SPANX-N, in other tissues as well.
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title_short	Evolutionary diversification of SPANX-N sperm protein gene structure and expression.
url	https://doi.org/10.1371/journal.pone.0000359 https://doaj.org/article/eb55759c6a8b47629bffb4a26a4f7f80 http://europepmc.org/articles/PMC1831492?pdf=render https://doaj.org/toc/1932-6203
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up_date	2024-07-04T02:12:18.963Z
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Evolutionary diversification of SPANX-N sperm protein gene structure and expression.

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