Slc43a3 is a regulator of free fatty acid flux1

Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known fu...
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Autor*in:	Kathrin B. Hasbargen [verfasserIn] Wen-Jun Shen [verfasserIn] Yiqiang Zhang [verfasserIn] Xiaoming Hou [verfasserIn] Wei Wang [verfasserIn] Qui Shuo [verfasserIn] David A. Bernlohr [verfasserIn] Salman Azhar [verfasserIn] Fredric B. Kraemer [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	lipid droplet adipose metabolism membrane transporters fatty acid uptake fatty acid efflux solute carrier family 43 member 3

Übergeordnetes Werk:	In: Journal of Lipid Research - Elsevier, 2021, 61(2020), 5, Seite 734-745
Übergeordnetes Werk:	volume:61 ; year:2020 ; number:5 ; pages:734-745

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1194/jlr.RA119000294

Katalog-ID:	DOAJ060220384

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source	In Journal of Lipid Research 61(2020), 5, Seite 734-745 volume:61 year:2020 number:5 pages:734-745
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authorswithroles_txt_mv	Kathrin B. Hasbargen @@aut@@ Wen-Jun Shen @@aut@@ Yiqiang Zhang @@aut@@ Xiaoming Hou @@aut@@ Wei Wang @@aut@@ Qui Shuo @@aut@@ David A. Bernlohr @@aut@@ Salman Azhar @@aut@@ Fredric B. Kraemer @@aut@@
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Hasbargen</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Slc43a3 is a regulator of free fatty acid flux1</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. 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callnumber-first	Q - Science
author	Kathrin B. Hasbargen
spellingShingle	Kathrin B. Hasbargen misc QD415-436 misc lipid droplet misc adipose metabolism misc membrane transporters misc fatty acid uptake misc fatty acid efflux misc solute carrier family 43 member 3 misc Biochemistry Slc43a3 is a regulator of free fatty acid flux1
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topic_title	QD415-436 Slc43a3 is a regulator of free fatty acid flux1 lipid droplet adipose metabolism membrane transporters fatty acid uptake fatty acid efflux solute carrier family 43 member 3
topic	misc QD415-436 misc lipid droplet misc adipose metabolism misc membrane transporters misc fatty acid uptake misc fatty acid efflux misc solute carrier family 43 member 3 misc Biochemistry
topic_unstemmed	misc QD415-436 misc lipid droplet misc adipose metabolism misc membrane transporters misc fatty acid uptake misc fatty acid efflux misc solute carrier family 43 member 3 misc Biochemistry
topic_browse	misc QD415-436 misc lipid droplet misc adipose metabolism misc membrane transporters misc fatty acid uptake misc fatty acid efflux misc solute carrier family 43 member 3 misc Biochemistry
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title	Slc43a3 is a regulator of free fatty acid flux1
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title_full	Slc43a3 is a regulator of free fatty acid flux1
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author_browse	Kathrin B. Hasbargen Wen-Jun Shen Yiqiang Zhang Xiaoming Hou Wei Wang Qui Shuo David A. Bernlohr Salman Azhar Fredric B. Kraemer
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title_sort	slc43a3 is a regulator of free fatty acid flux1
callnumber	QD415-436
title_auth	Slc43a3 is a regulator of free fatty acid flux1
abstract	Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known function were screened in subcutaneous adipose samples from obese patients before and after bariatric surgery using branched DNA methodology. Among the 33 solute carrier (SLC) transporter family members screened, the expression of 14 members showed significant changes before and after bariatric surgery. One of them, Slc43a3, increased about 2.5-fold after bariatric surgery. Further investigation demonstrated that Slc43a3 is highly expressed in murine adipose tissue and induced during adipocyte differentiation in primary preadipocytes and in OP9 cells. Knockdown of Slc43a3 with siRNA in differentiated OP9 adipocytes reduced both basal and forskolin-stimulated FA efflux, while also increasing FA uptake and lipid droplet accumulation. In contrast, overexpression of Slc43a3 decreased FA uptake in differentiated OP9 cells and resulted in decreased lipid droplet accumulation. Therefore, Slc43a3 seems to regulate FA flux in adipocytes, functioning as a positive regulator of FA efflux and as a negative regulator of FA uptake.
abstractGer	Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known function were screened in subcutaneous adipose samples from obese patients before and after bariatric surgery using branched DNA methodology. Among the 33 solute carrier (SLC) transporter family members screened, the expression of 14 members showed significant changes before and after bariatric surgery. One of them, Slc43a3, increased about 2.5-fold after bariatric surgery. Further investigation demonstrated that Slc43a3 is highly expressed in murine adipose tissue and induced during adipocyte differentiation in primary preadipocytes and in OP9 cells. Knockdown of Slc43a3 with siRNA in differentiated OP9 adipocytes reduced both basal and forskolin-stimulated FA efflux, while also increasing FA uptake and lipid droplet accumulation. In contrast, overexpression of Slc43a3 decreased FA uptake in differentiated OP9 cells and resulted in decreased lipid droplet accumulation. Therefore, Slc43a3 seems to regulate FA flux in adipocytes, functioning as a positive regulator of FA efflux and as a negative regulator of FA uptake.
abstract_unstemmed	Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known function were screened in subcutaneous adipose samples from obese patients before and after bariatric surgery using branched DNA methodology. Among the 33 solute carrier (SLC) transporter family members screened, the expression of 14 members showed significant changes before and after bariatric surgery. One of them, Slc43a3, increased about 2.5-fold after bariatric surgery. Further investigation demonstrated that Slc43a3 is highly expressed in murine adipose tissue and induced during adipocyte differentiation in primary preadipocytes and in OP9 cells. Knockdown of Slc43a3 with siRNA in differentiated OP9 adipocytes reduced both basal and forskolin-stimulated FA efflux, while also increasing FA uptake and lipid droplet accumulation. In contrast, overexpression of Slc43a3 decreased FA uptake in differentiated OP9 cells and resulted in decreased lipid droplet accumulation. Therefore, Slc43a3 seems to regulate FA flux in adipocytes, functioning as a positive regulator of FA efflux and as a negative regulator of FA uptake.
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title_short	Slc43a3 is a regulator of free fatty acid flux1
url	https://doi.org/10.1194/jlr.RA119000294 https://doaj.org/article/adee7867d43c4ae8b513887121686c5d http://www.sciencedirect.com/science/article/pii/S0022227520436223 https://doaj.org/toc/0022-2275
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author2	Wen-Jun Shen Yiqiang Zhang Xiaoming Hou Wei Wang Qui Shuo David A. Bernlohr Salman Azhar Fredric B. Kraemer
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up_date	2024-07-03T13:40:43.358Z
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Slc43a3 is a regulator of free fatty acid flux1

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