<i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice

<i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porp...
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Autor*in:	Teppei Okabe [verfasserIn] Yosuke Kamiya [verfasserIn] Takeshi Kikuchi [verfasserIn] Hisashi Goto [verfasserIn] Masayuki Umemura [verfasserIn] Yuki Suzuki [verfasserIn] Yoshihiko Sugita [verfasserIn] Yoshikazu Naiki [verfasserIn] Yoshiaki Hasegawa [verfasserIn] Jun-ichiro Hayashi [verfasserIn] Shotaro Kawamura [verfasserIn] Noritaka Sawada [verfasserIn] Yuhei Takayanagi [verfasserIn] Takeki Fujimura [verfasserIn] Naoya Higuchi [verfasserIn] Akio Mitani [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	pneumonia

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 22(2021), 23, p 12704
Übergeordnetes Werk:	volume:22 ; year:2021 ; number:23, p 12704

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms222312704

Katalog-ID:	DOAJ060791578

Internformat


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allfields	10.3390/ijms222312704 doi (DE-627)DOAJ060791578 (DE-599)DOAJb7d02c915511408593083144dc963fc5 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Teppei Okabe verfasserin aut <i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. <i<Porphyromonas gingivalis</i< <i<Streptococcus pneumoniae</i< pneumonia Biology (General) Chemistry Yosuke Kamiya verfasserin aut Takeshi Kikuchi verfasserin aut Hisashi Goto verfasserin aut Masayuki Umemura verfasserin aut Yuki Suzuki verfasserin aut Yoshihiko Sugita verfasserin aut Yoshikazu Naiki verfasserin aut Yoshiaki Hasegawa verfasserin aut Jun-ichiro Hayashi verfasserin aut Shotaro Kawamura verfasserin aut Noritaka Sawada verfasserin aut Yuhei Takayanagi verfasserin aut Takeki Fujimura verfasserin aut Naoya Higuchi verfasserin aut Akio Mitani verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 23, p 12704 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:23, p 12704 https://doi.org/10.3390/ijms222312704 kostenfrei https://doaj.org/article/b7d02c915511408593083144dc963fc5 kostenfrei https://www.mdpi.com/1422-0067/22/23/12704 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 23, p 12704
spelling	10.3390/ijms222312704 doi (DE-627)DOAJ060791578 (DE-599)DOAJb7d02c915511408593083144dc963fc5 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Teppei Okabe verfasserin aut <i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. <i<Porphyromonas gingivalis</i< <i<Streptococcus pneumoniae</i< pneumonia Biology (General) Chemistry Yosuke Kamiya verfasserin aut Takeshi Kikuchi verfasserin aut Hisashi Goto verfasserin aut Masayuki Umemura verfasserin aut Yuki Suzuki verfasserin aut Yoshihiko Sugita verfasserin aut Yoshikazu Naiki verfasserin aut Yoshiaki Hasegawa verfasserin aut Jun-ichiro Hayashi verfasserin aut Shotaro Kawamura verfasserin aut Noritaka Sawada verfasserin aut Yuhei Takayanagi verfasserin aut Takeki Fujimura verfasserin aut Naoya Higuchi verfasserin aut Akio Mitani verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 23, p 12704 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:23, p 12704 https://doi.org/10.3390/ijms222312704 kostenfrei https://doaj.org/article/b7d02c915511408593083144dc963fc5 kostenfrei https://www.mdpi.com/1422-0067/22/23/12704 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 23, p 12704
allfields_unstemmed	10.3390/ijms222312704 doi (DE-627)DOAJ060791578 (DE-599)DOAJb7d02c915511408593083144dc963fc5 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Teppei Okabe verfasserin aut <i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. <i<Porphyromonas gingivalis</i< <i<Streptococcus pneumoniae</i< pneumonia Biology (General) Chemistry Yosuke Kamiya verfasserin aut Takeshi Kikuchi verfasserin aut Hisashi Goto verfasserin aut Masayuki Umemura verfasserin aut Yuki Suzuki verfasserin aut Yoshihiko Sugita verfasserin aut Yoshikazu Naiki verfasserin aut Yoshiaki Hasegawa verfasserin aut Jun-ichiro Hayashi verfasserin aut Shotaro Kawamura verfasserin aut Noritaka Sawada verfasserin aut Yuhei Takayanagi verfasserin aut Takeki Fujimura verfasserin aut Naoya Higuchi verfasserin aut Akio Mitani verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 23, p 12704 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:23, p 12704 https://doi.org/10.3390/ijms222312704 kostenfrei https://doaj.org/article/b7d02c915511408593083144dc963fc5 kostenfrei https://www.mdpi.com/1422-0067/22/23/12704 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 23, p 12704
allfieldsGer	10.3390/ijms222312704 doi (DE-627)DOAJ060791578 (DE-599)DOAJb7d02c915511408593083144dc963fc5 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Teppei Okabe verfasserin aut <i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. <i<Porphyromonas gingivalis</i< <i<Streptococcus pneumoniae</i< pneumonia Biology (General) Chemistry Yosuke Kamiya verfasserin aut Takeshi Kikuchi verfasserin aut Hisashi Goto verfasserin aut Masayuki Umemura verfasserin aut Yuki Suzuki verfasserin aut Yoshihiko Sugita verfasserin aut Yoshikazu Naiki verfasserin aut Yoshiaki Hasegawa verfasserin aut Jun-ichiro Hayashi verfasserin aut Shotaro Kawamura verfasserin aut Noritaka Sawada verfasserin aut Yuhei Takayanagi verfasserin aut Takeki Fujimura verfasserin aut Naoya Higuchi verfasserin aut Akio Mitani verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 23, p 12704 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:23, p 12704 https://doi.org/10.3390/ijms222312704 kostenfrei https://doaj.org/article/b7d02c915511408593083144dc963fc5 kostenfrei https://www.mdpi.com/1422-0067/22/23/12704 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 23, p 12704
allfieldsSound	10.3390/ijms222312704 doi (DE-627)DOAJ060791578 (DE-599)DOAJb7d02c915511408593083144dc963fc5 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Teppei Okabe verfasserin aut <i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia. <i<Porphyromonas gingivalis</i< <i<Streptococcus pneumoniae</i< pneumonia Biology (General) Chemistry Yosuke Kamiya verfasserin aut Takeshi Kikuchi verfasserin aut Hisashi Goto verfasserin aut Masayuki Umemura verfasserin aut Yuki Suzuki verfasserin aut Yoshihiko Sugita verfasserin aut Yoshikazu Naiki verfasserin aut Yoshiaki Hasegawa verfasserin aut Jun-ichiro Hayashi verfasserin aut Shotaro Kawamura verfasserin aut Noritaka Sawada verfasserin aut Yuhei Takayanagi verfasserin aut Takeki Fujimura verfasserin aut Naoya Higuchi verfasserin aut Akio Mitani verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 23, p 12704 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:23, p 12704 https://doi.org/10.3390/ijms222312704 kostenfrei https://doaj.org/article/b7d02c915511408593083144dc963fc5 kostenfrei https://www.mdpi.com/1422-0067/22/23/12704 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 23, p 12704
language	English
source	In International Journal of Molecular Sciences 22(2021), 23, p 12704 volume:22 year:2021 number:23, p 12704
sourceStr	In International Journal of Molecular Sciences 22(2021), 23, p 12704 volume:22 year:2021 number:23, p 12704
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Porphyromonas gingivalis</i< <i<Streptococcus pneumoniae</i< pneumonia Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Teppei Okabe @@aut@@ Yosuke Kamiya @@aut@@ Takeshi Kikuchi @@aut@@ Hisashi Goto @@aut@@ Masayuki Umemura @@aut@@ Yuki Suzuki @@aut@@ Yoshihiko Sugita @@aut@@ Yoshikazu Naiki @@aut@@ Yoshiaki Hasegawa @@aut@@ Jun-ichiro Hayashi @@aut@@ Shotaro Kawamura @@aut@@ Noritaka Sawada @@aut@@ Yuhei Takayanagi @@aut@@ Takeki Fujimura @@aut@@ Naoya Higuchi @@aut@@ Akio Mitani @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ060791578
language_de	englisch
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callnumber-first	Q - Science
author	Teppei Okabe
spellingShingle	Teppei Okabe misc QH301-705.5 misc QD1-999 misc <i<Porphyromonas gingivalis</i< misc <i<Streptococcus pneumoniae</i< misc pneumonia misc Biology (General) misc Chemistry <i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice
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topic_title	QH301-705.5 QD1-999 <i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice <i<Porphyromonas gingivalis</i< <i<Streptococcus pneumoniae</i< pneumonia
topic	misc QH301-705.5 misc QD1-999 misc <i<Porphyromonas gingivalis</i< misc <i<Streptococcus pneumoniae</i< misc pneumonia misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc <i<Porphyromonas gingivalis</i< misc <i<Streptococcus pneumoniae</i< misc pneumonia misc Biology (General) misc Chemistry
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title	<i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice
ctrlnum	(DE-627)DOAJ060791578 (DE-599)DOAJb7d02c915511408593083144dc963fc5
title_full	<i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice
author_sort	Teppei Okabe
journal	International Journal of Molecular Sciences
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author_browse	Teppei Okabe Yosuke Kamiya Takeshi Kikuchi Hisashi Goto Masayuki Umemura Yuki Suzuki Yoshihiko Sugita Yoshikazu Naiki Yoshiaki Hasegawa Jun-ichiro Hayashi Shotaro Kawamura Noritaka Sawada Yuhei Takayanagi Takeki Fujimura Naoya Higuchi Akio Mitani
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author-letter	Teppei Okabe
doi_str_mv	10.3390/ijms222312704
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title_sort	<i<porphyromonas gingivalis</i< components/secretions synergistically enhance pneumonia caused by <i<streptococcus pneumoniae</i< in mice
callnumber	QH301-705.5
title_auth	<i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice
abstract	<i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia.
abstractGer	<i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia.
abstract_unstemmed	<i<Streptococcus pneumoniae</i< is an important causative organism of respiratory tract infections. Although periodontal bacteria have been shown to influence respiratory infections such as aspiration pneumonia, the synergistic effect of <i<S. pneumoniae</i< and <i<Porphyromonas gingivalis</i<, a periodontopathic bacterium, on pneumococcal infections is unclear. To investigate whether <i<P. gingivalis</i< accelerates pneumococcal infections, we tested the effects of inoculating <i<P. gingivalis</i< culture supernatant (PgSup) into <i<S. pneumoniae</i<-infected mice. Mice were intratracheally injected with <i<S. pneumoniae</i< and PgSup to induce pneumonia, and lung histopathological sections and the absolute number and frequency of neutrophils and macrophages in the lung were analyzed. Proinflammatory cytokine/chemokine expression was examined by qPCR and ELISA. Inflammatory cell infiltration was observed in <i<S. pneumoniae</i<-infected mice and <i<S. pnemoniae</i< and PgSup mixed-infected mice, and mixed-infected mice showed more pronounced inflammation in lung. The ratios of monocytes/macrophages and neutrophils were not significantly different between the lungs of <i<S. pneumoniae</i<-infected mice and those of mixed-infected mice. PgSup synergistically increased TNF-α expression/production and IL-17 production compared with <i<S. pneumoniae</i< infection alone. We demonstrated that PgSup enhanced inflammation in pneumonia caused by <i<S. pneumoniae</i<, suggesting that virulence factors produced by <i<P. gingivalis</i< are involved in the exacerbation of respiratory tract infections such as aspiration pneumonia.
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container_issue	23, p 12704
title_short	<i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice
url	https://doi.org/10.3390/ijms222312704 https://doaj.org/article/b7d02c915511408593083144dc963fc5 https://www.mdpi.com/1422-0067/22/23/12704 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2	Yosuke Kamiya Takeshi Kikuchi Hisashi Goto Masayuki Umemura Yuki Suzuki Yoshihiko Sugita Yoshikazu Naiki Yoshiaki Hasegawa Jun-ichiro Hayashi Shotaro Kawamura Noritaka Sawada Yuhei Takayanagi Takeki Fujimura Naoya Higuchi Akio Mitani
author2Str	Yosuke Kamiya Takeshi Kikuchi Hisashi Goto Masayuki Umemura Yuki Suzuki Yoshihiko Sugita Yoshikazu Naiki Yoshiaki Hasegawa Jun-ichiro Hayashi Shotaro Kawamura Noritaka Sawada Yuhei Takayanagi Takeki Fujimura Naoya Higuchi Akio Mitani
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<i<Porphyromonas gingivalis</i< Components/Secretions Synergistically Enhance Pneumonia Caused by <i<Streptococcus pneumoniae</i< in Mice

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