No limit in interspecific hybridization in schistosomes: observation from a case report

Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Depaquit Jérôme [verfasserIn] Akhoundi Mohammad [verfasserIn] Haouchine Djamel [verfasserIn] Mantelet Stéphane [verfasserIn] Izri Arezki [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance

Übergeordnetes Werk:	In: Parasite - EDP Sciences, 2015, 26, p 10(2019)
Übergeordnetes Werk:	volume:26, p 10 ; year:2019

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1051/parasite/2019010

Katalog-ID:	DOAJ061321540

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allfields	10.1051/parasite/2019010 doi (DE-627)DOAJ061321540 (DE-599)DOAJea276a868b474e1c9883fc9281a93a87 DE-627 ger DE-627 rakwb eng RC109-216 Depaquit Jérôme verfasserin aut No limit in interspecific hybridization in schistosomes: observation from a case report 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future. Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance Infectious and parasitic diseases Akhoundi Mohammad verfasserin aut Haouchine Djamel verfasserin aut Mantelet Stéphane verfasserin aut Izri Arezki verfasserin aut In Parasite EDP Sciences, 2015 26, p 10(2019) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:26, p 10 year:2019 https://doi.org/10.1051/parasite/2019010 kostenfrei https://doaj.org/article/ea276a868b474e1c9883fc9281a93a87 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180149/parasite180149.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26, p 10 2019
spelling	10.1051/parasite/2019010 doi (DE-627)DOAJ061321540 (DE-599)DOAJea276a868b474e1c9883fc9281a93a87 DE-627 ger DE-627 rakwb eng RC109-216 Depaquit Jérôme verfasserin aut No limit in interspecific hybridization in schistosomes: observation from a case report 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future. Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance Infectious and parasitic diseases Akhoundi Mohammad verfasserin aut Haouchine Djamel verfasserin aut Mantelet Stéphane verfasserin aut Izri Arezki verfasserin aut In Parasite EDP Sciences, 2015 26, p 10(2019) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:26, p 10 year:2019 https://doi.org/10.1051/parasite/2019010 kostenfrei https://doaj.org/article/ea276a868b474e1c9883fc9281a93a87 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180149/parasite180149.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26, p 10 2019
allfields_unstemmed	10.1051/parasite/2019010 doi (DE-627)DOAJ061321540 (DE-599)DOAJea276a868b474e1c9883fc9281a93a87 DE-627 ger DE-627 rakwb eng RC109-216 Depaquit Jérôme verfasserin aut No limit in interspecific hybridization in schistosomes: observation from a case report 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future. Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance Infectious and parasitic diseases Akhoundi Mohammad verfasserin aut Haouchine Djamel verfasserin aut Mantelet Stéphane verfasserin aut Izri Arezki verfasserin aut In Parasite EDP Sciences, 2015 26, p 10(2019) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:26, p 10 year:2019 https://doi.org/10.1051/parasite/2019010 kostenfrei https://doaj.org/article/ea276a868b474e1c9883fc9281a93a87 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180149/parasite180149.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26, p 10 2019
allfieldsGer	10.1051/parasite/2019010 doi (DE-627)DOAJ061321540 (DE-599)DOAJea276a868b474e1c9883fc9281a93a87 DE-627 ger DE-627 rakwb eng RC109-216 Depaquit Jérôme verfasserin aut No limit in interspecific hybridization in schistosomes: observation from a case report 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future. Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance Infectious and parasitic diseases Akhoundi Mohammad verfasserin aut Haouchine Djamel verfasserin aut Mantelet Stéphane verfasserin aut Izri Arezki verfasserin aut In Parasite EDP Sciences, 2015 26, p 10(2019) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:26, p 10 year:2019 https://doi.org/10.1051/parasite/2019010 kostenfrei https://doaj.org/article/ea276a868b474e1c9883fc9281a93a87 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180149/parasite180149.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26, p 10 2019
allfieldsSound	10.1051/parasite/2019010 doi (DE-627)DOAJ061321540 (DE-599)DOAJea276a868b474e1c9883fc9281a93a87 DE-627 ger DE-627 rakwb eng RC109-216 Depaquit Jérôme verfasserin aut No limit in interspecific hybridization in schistosomes: observation from a case report 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future. Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance Infectious and parasitic diseases Akhoundi Mohammad verfasserin aut Haouchine Djamel verfasserin aut Mantelet Stéphane verfasserin aut Izri Arezki verfasserin aut In Parasite EDP Sciences, 2015 26, p 10(2019) (DE-627)527642398 (DE-600)2278575-9 17761042 nnns volume:26, p 10 year:2019 https://doi.org/10.1051/parasite/2019010 kostenfrei https://doaj.org/article/ea276a868b474e1c9883fc9281a93a87 kostenfrei https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180149/parasite180149.html kostenfrei https://doaj.org/toc/1776-1042 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26, p 10 2019
language	English
source	In Parasite 26, p 10(2019) volume:26, p 10 year:2019
sourceStr	In Parasite 26, p 10(2019) volume:26, p 10 year:2019
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topic_facet	Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance Infectious and parasitic diseases
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authorswithroles_txt_mv	Depaquit Jérôme @@aut@@ Akhoundi Mohammad @@aut@@ Haouchine Djamel @@aut@@ Mantelet Stéphane @@aut@@ Izri Arezki @@aut@@
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callnumber-first	R - Medicine
author	Depaquit Jérôme
spellingShingle	Depaquit Jérôme misc RC109-216 misc Schistosoma mansoni misc S. haematobium misc Hematuria misc Hybrid misc phylogenetic distance misc Infectious and parasitic diseases No limit in interspecific hybridization in schistosomes: observation from a case report
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topic_title	RC109-216 No limit in interspecific hybridization in schistosomes: observation from a case report Schistosoma mansoni S. haematobium Hematuria Hybrid phylogenetic distance
topic	misc RC109-216 misc Schistosoma mansoni misc S. haematobium misc Hematuria misc Hybrid misc phylogenetic distance misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc Schistosoma mansoni misc S. haematobium misc Hematuria misc Hybrid misc phylogenetic distance misc Infectious and parasitic diseases
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title	No limit in interspecific hybridization in schistosomes: observation from a case report
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title_full	No limit in interspecific hybridization in schistosomes: observation from a case report
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author_browse	Depaquit Jérôme Akhoundi Mohammad Haouchine Djamel Mantelet Stéphane Izri Arezki
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title_sort	no limit in interspecific hybridization in schistosomes: observation from a case report
callnumber	RC109-216
title_auth	No limit in interspecific hybridization in schistosomes: observation from a case report
abstract	Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future.
abstractGer	Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future.
abstract_unstemmed	Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related Schistosoma species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from Côte d’Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150 μm long × 62 μm wide) with a lateral spine (25 μm), identified morphologically as Schistosoma mansoni. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient’s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many S. mansoni eggs were found again in the patient’s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between S. mansoni and S. haematobium. Similarly, we identified a unique S. mansoni COI sequence for the two eggs, with 99.1% homology with the S. mansoni reference sequence. Consequently, this case was the result of hybridization between an S. haematobium male and an S. mansoni female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future.
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title_short	No limit in interspecific hybridization in schistosomes: observation from a case report
url	https://doi.org/10.1051/parasite/2019010 https://doaj.org/article/ea276a868b474e1c9883fc9281a93a87 https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180149/parasite180149.html https://doaj.org/toc/1776-1042
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No limit in interspecific hybridization in schistosomes: observation from a case report

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