Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape

Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production. Ausführliche Beschreibung

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Autor*in:	Liangrong Jiang [verfasserIn] Hui Zhong [verfasserIn] Xianbin Jiang [verfasserIn] Jiaoping Zhang [verfasserIn] Rongyu Huang [verfasserIn] Furong Liao [verfasserIn] Yaqin Deng [verfasserIn] Qingqing Liu [verfasserIn] Yumin Huang [verfasserIn] Houcong Wang [verfasserIn] Yi Tao [verfasserIn] Jingsheng Zheng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 12(2022)
Übergeordnetes Werk:	volume:12 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2021.814928

Katalog-ID:	DOAJ061386022

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allfields	10.3389/fpls.2021.814928 doi (DE-627)DOAJ061386022 (DE-599)DOAJ147dfc340cae41fa869bab07347461a3 DE-627 ger DE-627 rakwb eng SB1-1110 Liangrong Jiang verfasserin aut Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production. rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection Plant culture Hui Zhong verfasserin aut Xianbin Jiang verfasserin aut Jiaoping Zhang verfasserin aut Rongyu Huang verfasserin aut Furong Liao verfasserin aut Yaqin Deng verfasserin aut Qingqing Liu verfasserin aut Yumin Huang verfasserin aut Houcong Wang verfasserin aut Yi Tao verfasserin aut Jingsheng Zheng verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.814928 kostenfrei https://doaj.org/article/147dfc340cae41fa869bab07347461a3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.814928/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
spelling	10.3389/fpls.2021.814928 doi (DE-627)DOAJ061386022 (DE-599)DOAJ147dfc340cae41fa869bab07347461a3 DE-627 ger DE-627 rakwb eng SB1-1110 Liangrong Jiang verfasserin aut Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production. rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection Plant culture Hui Zhong verfasserin aut Xianbin Jiang verfasserin aut Jiaoping Zhang verfasserin aut Rongyu Huang verfasserin aut Furong Liao verfasserin aut Yaqin Deng verfasserin aut Qingqing Liu verfasserin aut Yumin Huang verfasserin aut Houcong Wang verfasserin aut Yi Tao verfasserin aut Jingsheng Zheng verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.814928 kostenfrei https://doaj.org/article/147dfc340cae41fa869bab07347461a3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.814928/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfields_unstemmed	10.3389/fpls.2021.814928 doi (DE-627)DOAJ061386022 (DE-599)DOAJ147dfc340cae41fa869bab07347461a3 DE-627 ger DE-627 rakwb eng SB1-1110 Liangrong Jiang verfasserin aut Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production. rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection Plant culture Hui Zhong verfasserin aut Xianbin Jiang verfasserin aut Jiaoping Zhang verfasserin aut Rongyu Huang verfasserin aut Furong Liao verfasserin aut Yaqin Deng verfasserin aut Qingqing Liu verfasserin aut Yumin Huang verfasserin aut Houcong Wang verfasserin aut Yi Tao verfasserin aut Jingsheng Zheng verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.814928 kostenfrei https://doaj.org/article/147dfc340cae41fa869bab07347461a3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.814928/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfieldsGer	10.3389/fpls.2021.814928 doi (DE-627)DOAJ061386022 (DE-599)DOAJ147dfc340cae41fa869bab07347461a3 DE-627 ger DE-627 rakwb eng SB1-1110 Liangrong Jiang verfasserin aut Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production. rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection Plant culture Hui Zhong verfasserin aut Xianbin Jiang verfasserin aut Jiaoping Zhang verfasserin aut Rongyu Huang verfasserin aut Furong Liao verfasserin aut Yaqin Deng verfasserin aut Qingqing Liu verfasserin aut Yumin Huang verfasserin aut Houcong Wang verfasserin aut Yi Tao verfasserin aut Jingsheng Zheng verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.814928 kostenfrei https://doaj.org/article/147dfc340cae41fa869bab07347461a3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.814928/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfieldsSound	10.3389/fpls.2021.814928 doi (DE-627)DOAJ061386022 (DE-599)DOAJ147dfc340cae41fa869bab07347461a3 DE-627 ger DE-627 rakwb eng SB1-1110 Liangrong Jiang verfasserin aut Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production. rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection Plant culture Hui Zhong verfasserin aut Xianbin Jiang verfasserin aut Jiaoping Zhang verfasserin aut Rongyu Huang verfasserin aut Furong Liao verfasserin aut Yaqin Deng verfasserin aut Qingqing Liu verfasserin aut Yumin Huang verfasserin aut Houcong Wang verfasserin aut Yi Tao verfasserin aut Jingsheng Zheng verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.814928 kostenfrei https://doaj.org/article/147dfc340cae41fa869bab07347461a3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.814928/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
language	English
source	In Frontiers in Plant Science 12(2022) volume:12 year:2022
sourceStr	In Frontiers in Plant Science 12(2022) volume:12 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection Plant culture
isfreeaccess_bool	true
container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Liangrong Jiang @@aut@@ Hui Zhong @@aut@@ Xianbin Jiang @@aut@@ Jiaoping Zhang @@aut@@ Rongyu Huang @@aut@@ Furong Liao @@aut@@ Yaqin Deng @@aut@@ Qingqing Liu @@aut@@ Yumin Huang @@aut@@ Houcong Wang @@aut@@ Yi Tao @@aut@@ Jingsheng Zheng @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	662359240
id	DOAJ061386022
language_de	englisch
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Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. 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callnumber-first	S - Agriculture
author	Liangrong Jiang
spellingShingle	Liangrong Jiang misc SB1-1110 misc rice (Oryza sativa L.) misc percentage of grains with chalkiness misc degree of endosperm chalkiness misc grain shape misc near-isogenic lines misc molecular marker-assisted selection misc Plant culture Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape
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topic_title	SB1-1110 Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape rice (Oryza sativa L.) percentage of grains with chalkiness degree of endosperm chalkiness grain shape near-isogenic lines molecular marker-assisted selection
topic	misc SB1-1110 misc rice (Oryza sativa L.) misc percentage of grains with chalkiness misc degree of endosperm chalkiness misc grain shape misc near-isogenic lines misc molecular marker-assisted selection misc Plant culture
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title	Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape
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title_full	Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape
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author_browse	Liangrong Jiang Hui Zhong Xianbin Jiang Jiaoping Zhang Rongyu Huang Furong Liao Yaqin Deng Qingqing Liu Yumin Huang Houcong Wang Yi Tao Jingsheng Zheng
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title_sort	identification and pleiotropic effect analysis of gse5 on rice chalkiness and grain shape
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title_auth	Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape
abstract	Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production.
abstractGer	Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production.
abstract_unstemmed	Chalkiness is one of several major restricting factors for the improvement of rice quality. Although many chalkiness-related quantitative trait loci have been mapped, only a small number of genes have been cloned to date. In this study, the candidate gene GSE5 of a major quantitative trait locus (QTL) for rice chalkiness, qDEC5, was identified by map-based cloning. Phenotyping and haplotype analysis of proActin:GSE5 transgenic line, gse5-cr mutant, and 69 rice varieties further confirmed that GSE5 had the pleiotropic effects and regulated both chalkiness and grain shape. Genetic analysis showed GSE5 was a dominant gene for grain length and a semi-dominant gene for grain width and chalkiness. The DNA interval closely linked to GSE5 was introgressed to Zhenshan 97B (ZB) based on molecular marker-assisted selection, and the improved ZB showed lower chalkiness and longer but smaller grains, which showed that GSE5 played an important role in breeding rice varieties with high yield and good quality. Transcriptomics, proteomics, and qRT-PCR analyses showed that thirty-nine genes associated with carbon and protein metabolism are regulated by GSE5 to affect the formation of chalkiness, including some newly discovered genes, such as OsCESA9, OsHSP70, OsTPS8, OsPFK04, OsSTA1, OsERdj3A, etc. The low-chalkiness lines showed higher amino sugar and nucleotide sugar metabolism at 10 days after pollination (DAP), lower carbohydrate metabolism at 15 DAP, and lower protein metabolism at 10 and 15 DAP. With heat shock at 34/30°C, rice chalkiness increased significantly; OsDjC10 and OsSUS3 were upregulated at 6 and 12 DAP, respectively, and OsGSTL2 was downregulated at 12 DAP. Our results identified the function and pleiotropic effects of qDEC5 dissected its genetic characteristics and the expression profiles of the genes affecting the chalkiness formation, and provided a theoretical basis and application value to harmoniously pursue high yield and good quality in rice production.
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title_short	Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape
url	https://doi.org/10.3389/fpls.2021.814928 https://doaj.org/article/147dfc340cae41fa869bab07347461a3 https://www.frontiersin.org/articles/10.3389/fpls.2021.814928/full https://doaj.org/toc/1664-462X
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Identification and Pleiotropic Effect Analysis of GSE5 on Rice Chalkiness and Grain Shape

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