Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice

AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established...
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Gespeichert in:

Autor*in:	Yi Zhang [verfasserIn] Ding-Ying Liao [verfasserIn] Jian-Ming Wang [verfasserIn] Li-Jun Wang [verfasserIn] Xi-Ting Yang [verfasserIn] Ai-Yi Zhou [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization

Übergeordnetes Werk:	In: International Journal of Ophthalmology - Press of International Journal of Ophthalmology (IJO PRESS), 2016, 15(2022), 2, Seite 189-196
Übergeordnetes Werk:	volume:15 ; year:2022 ; number:2 ; pages:189-196

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.18240/ijo.2022.02.01

Katalog-ID:	DOAJ061502146

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520			\|a AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells.
650		4	\|a placental growth factor
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650		4	\|a epithelial mesenchymal transformation
650		4	\|a choroidal neovascularization
653		0	\|a Ophthalmology
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700	0		\|a Jian-Ming Wang \|e verfasserin \|4 aut
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700	0		\|a Xi-Ting Yang \|e verfasserin \|4 aut
700	0		\|a Ai-Yi Zhou \|e verfasserin \|4 aut
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allfields	10.18240/ijo.2022.02.01 doi (DE-627)DOAJ061502146 (DE-599)DOAJe229db7a76fb443080518d1dd1f92a9d DE-627 ger DE-627 rakwb eng RE1-994 Yi Zhang verfasserin aut Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells. placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization Ophthalmology Ding-Ying Liao verfasserin aut Jian-Ming Wang verfasserin aut Li-Jun Wang verfasserin aut Xi-Ting Yang verfasserin aut Ai-Yi Zhou verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 15(2022), 2, Seite 189-196 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:15 year:2022 number:2 pages:189-196 https://doi.org/10.18240/ijo.2022.02.01 kostenfrei https://doaj.org/article/e229db7a76fb443080518d1dd1f92a9d kostenfrei http://ies.ijo.cn/en_publish/2022/2/20220201.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 2 189-196
spelling	10.18240/ijo.2022.02.01 doi (DE-627)DOAJ061502146 (DE-599)DOAJe229db7a76fb443080518d1dd1f92a9d DE-627 ger DE-627 rakwb eng RE1-994 Yi Zhang verfasserin aut Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells. placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization Ophthalmology Ding-Ying Liao verfasserin aut Jian-Ming Wang verfasserin aut Li-Jun Wang verfasserin aut Xi-Ting Yang verfasserin aut Ai-Yi Zhou verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 15(2022), 2, Seite 189-196 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:15 year:2022 number:2 pages:189-196 https://doi.org/10.18240/ijo.2022.02.01 kostenfrei https://doaj.org/article/e229db7a76fb443080518d1dd1f92a9d kostenfrei http://ies.ijo.cn/en_publish/2022/2/20220201.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 2 189-196
allfields_unstemmed	10.18240/ijo.2022.02.01 doi (DE-627)DOAJ061502146 (DE-599)DOAJe229db7a76fb443080518d1dd1f92a9d DE-627 ger DE-627 rakwb eng RE1-994 Yi Zhang verfasserin aut Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells. placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization Ophthalmology Ding-Ying Liao verfasserin aut Jian-Ming Wang verfasserin aut Li-Jun Wang verfasserin aut Xi-Ting Yang verfasserin aut Ai-Yi Zhou verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 15(2022), 2, Seite 189-196 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:15 year:2022 number:2 pages:189-196 https://doi.org/10.18240/ijo.2022.02.01 kostenfrei https://doaj.org/article/e229db7a76fb443080518d1dd1f92a9d kostenfrei http://ies.ijo.cn/en_publish/2022/2/20220201.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 2 189-196
allfieldsGer	10.18240/ijo.2022.02.01 doi (DE-627)DOAJ061502146 (DE-599)DOAJe229db7a76fb443080518d1dd1f92a9d DE-627 ger DE-627 rakwb eng RE1-994 Yi Zhang verfasserin aut Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells. placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization Ophthalmology Ding-Ying Liao verfasserin aut Jian-Ming Wang verfasserin aut Li-Jun Wang verfasserin aut Xi-Ting Yang verfasserin aut Ai-Yi Zhou verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 15(2022), 2, Seite 189-196 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:15 year:2022 number:2 pages:189-196 https://doi.org/10.18240/ijo.2022.02.01 kostenfrei https://doaj.org/article/e229db7a76fb443080518d1dd1f92a9d kostenfrei http://ies.ijo.cn/en_publish/2022/2/20220201.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 2 189-196
allfieldsSound	10.18240/ijo.2022.02.01 doi (DE-627)DOAJ061502146 (DE-599)DOAJe229db7a76fb443080518d1dd1f92a9d DE-627 ger DE-627 rakwb eng RE1-994 Yi Zhang verfasserin aut Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells. placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization Ophthalmology Ding-Ying Liao verfasserin aut Jian-Ming Wang verfasserin aut Li-Jun Wang verfasserin aut Xi-Ting Yang verfasserin aut Ai-Yi Zhou verfasserin aut In International Journal of Ophthalmology Press of International Journal of Ophthalmology (IJO PRESS), 2016 15(2022), 2, Seite 189-196 (DE-627)718635906 (DE-600)2663246-9 22274898 nnns volume:15 year:2022 number:2 pages:189-196 https://doi.org/10.18240/ijo.2022.02.01 kostenfrei https://doaj.org/article/e229db7a76fb443080518d1dd1f92a9d kostenfrei http://ies.ijo.cn/en_publish/2022/2/20220201.pdf kostenfrei https://doaj.org/toc/2222-3959 Journal toc kostenfrei https://doaj.org/toc/2227-4898 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2022 2 189-196
language	English
source	In International Journal of Ophthalmology 15(2022), 2, Seite 189-196 volume:15 year:2022 number:2 pages:189-196
sourceStr	In International Journal of Ophthalmology 15(2022), 2, Seite 189-196 volume:15 year:2022 number:2 pages:189-196
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topic_facet	placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization Ophthalmology
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authorswithroles_txt_mv	Yi Zhang @@aut@@ Ding-Ying Liao @@aut@@ Jian-Ming Wang @@aut@@ Li-Jun Wang @@aut@@ Xi-Ting Yang @@aut@@ Ai-Yi Zhou @@aut@@
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callnumber-first	R - Medicine
author	Yi Zhang
spellingShingle	Yi Zhang misc RE1-994 misc placental growth factor misc subretinal fibrosis misc epithelial mesenchymal transformation misc choroidal neovascularization misc Ophthalmology Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice
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topic_title	RE1-994 Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice placental growth factor subretinal fibrosis epithelial mesenchymal transformation choroidal neovascularization
topic	misc RE1-994 misc placental growth factor misc subretinal fibrosis misc epithelial mesenchymal transformation misc choroidal neovascularization misc Ophthalmology
topic_unstemmed	misc RE1-994 misc placental growth factor misc subretinal fibrosis misc epithelial mesenchymal transformation misc choroidal neovascularization misc Ophthalmology
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hierarchy_parent_title	International Journal of Ophthalmology
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title	Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice
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title_full	Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice
author_sort	Yi Zhang
journal	International Journal of Ophthalmology
journalStr	International Journal of Ophthalmology
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author_browse	Yi Zhang Ding-Ying Liao Jian-Ming Wang Li-Jun Wang Xi-Ting Yang Ai-Yi Zhou
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class	RE1-994
format_se	Elektronische Aufsätze
author-letter	Yi Zhang
doi_str_mv	10.18240/ijo.2022.02.01
author2-role	verfasserin
title_sort	inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice
callnumber	RE1-994
title_auth	Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice
abstract	AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells.
abstractGer	AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells.
abstract_unstemmed	AIM: To investigate whether anti-placental growth factor (PGF) can inhibit subretinal fibrosis and whether this effect is mediated by the inhibitory effect of PGF on epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells. METHODS: Subretinal fibrosis model was established in laser induced choroidal neovascularization (CNV) mice on day 21 after laser photocoagulation. Immunofluorescence staining (IFS) of cryosections and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of PGF. IFS of whole choroidal flat-mounts was used to detect the degree of subretinal fibrosis. IFS of cryosections and ELISA were used to detect the expression of EMT related indicators in subretinal fibrosis lesions. RESULTS: The expression of PGF protein in subretinal fibrosis lesions was significantly up-regulated (P<0.05), and mainly co-stained with pan-cytokeratin labeled RPE cells. Intravitreal injection of anti-PGF neutralizing antibody reduced the area of subretinal fibrosis and the ratio of fibrotic/angiogenic area significantly at the concentrations of 0.25, 0.5, 1.0, and 2.0 μg/μL (all P<0.05). The expression of E-cadherin in the local RPE cells decreased, while α-SMA increased significantly in subretinal fibrosis lesions, and the application of anti-PGF neutralizing antibody could reverse these changes (P<0.05). CONCLUSION: The expression of PGF is up-regulated in the lesion site of subretinal fibrosis and mainly expressed in RPE cells. Intravitreal injection of anti-PGF neutralizing antibody can significantly inhibit the degree of subretinal fibrosis in CNV mice, and this effect may be mediated by the inhibition of PGF on EMT of RPE cells.
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title_short	Inhibitory effect on subretinal fibrosis by anti-placental growth factor treatment in a laser-induced choroidal neovascularization model in mice
url	https://doi.org/10.18240/ijo.2022.02.01 https://doaj.org/article/e229db7a76fb443080518d1dd1f92a9d http://ies.ijo.cn/en_publish/2022/2/20220201.pdf https://doaj.org/toc/2222-3959 https://doaj.org/toc/2227-4898
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author2	Ding-Ying Liao Jian-Ming Wang Li-Jun Wang Xi-Ting Yang Ai-Yi Zhou
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