Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces...
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Autor*in:	Pedro Beltrao [verfasserIn] Jonathan C Trinidad [verfasserIn] Dorothea Fiedler [verfasserIn] Assen Roguev [verfasserIn] Wendell A Lim [verfasserIn] Kevan M Shokat [verfasserIn] Alma L Burlingame [verfasserIn] Nevan J Krogan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Übergeordnetes Werk:	In: PLoS Biology - Public Library of Science (PLoS), 2003, 7(2009), 6, p e1000134
Übergeordnetes Werk:	volume:7 ; year:2009 ; number:6, p e1000134

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1371/journal.pbio.1000134

Katalog-ID:	DOAJ061974323

Internformat


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allfields	10.1371/journal.pbio.1000134 doi (DE-627)DOAJ061974323 (DE-599)DOAJ5e8bdd4a44c841e89f6e26ba4767285d DE-627 ger DE-627 rakwb eng QH301-705.5 Pedro Beltrao verfasserin aut Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity. Biology (General) Jonathan C Trinidad verfasserin aut Dorothea Fiedler verfasserin aut Assen Roguev verfasserin aut Wendell A Lim verfasserin aut Kevan M Shokat verfasserin aut Alma L Burlingame verfasserin aut Nevan J Krogan verfasserin aut In PLoS Biology Public Library of Science (PLoS), 2003 7(2009), 6, p e1000134 (DE-627)373755597 (DE-600)2126773-X 15457885 nnns volume:7 year:2009 number:6, p e1000134 https://doi.org/10.1371/journal.pbio.1000134 kostenfrei https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d kostenfrei https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547744/?tool=EBI kostenfrei https://doaj.org/toc/1544-9173 Journal toc kostenfrei https://doaj.org/toc/1545-7885 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 6, p e1000134
spelling	10.1371/journal.pbio.1000134 doi (DE-627)DOAJ061974323 (DE-599)DOAJ5e8bdd4a44c841e89f6e26ba4767285d DE-627 ger DE-627 rakwb eng QH301-705.5 Pedro Beltrao verfasserin aut Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity. Biology (General) Jonathan C Trinidad verfasserin aut Dorothea Fiedler verfasserin aut Assen Roguev verfasserin aut Wendell A Lim verfasserin aut Kevan M Shokat verfasserin aut Alma L Burlingame verfasserin aut Nevan J Krogan verfasserin aut In PLoS Biology Public Library of Science (PLoS), 2003 7(2009), 6, p e1000134 (DE-627)373755597 (DE-600)2126773-X 15457885 nnns volume:7 year:2009 number:6, p e1000134 https://doi.org/10.1371/journal.pbio.1000134 kostenfrei https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d kostenfrei https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547744/?tool=EBI kostenfrei https://doaj.org/toc/1544-9173 Journal toc kostenfrei https://doaj.org/toc/1545-7885 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 6, p e1000134
allfields_unstemmed	10.1371/journal.pbio.1000134 doi (DE-627)DOAJ061974323 (DE-599)DOAJ5e8bdd4a44c841e89f6e26ba4767285d DE-627 ger DE-627 rakwb eng QH301-705.5 Pedro Beltrao verfasserin aut Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity. Biology (General) Jonathan C Trinidad verfasserin aut Dorothea Fiedler verfasserin aut Assen Roguev verfasserin aut Wendell A Lim verfasserin aut Kevan M Shokat verfasserin aut Alma L Burlingame verfasserin aut Nevan J Krogan verfasserin aut In PLoS Biology Public Library of Science (PLoS), 2003 7(2009), 6, p e1000134 (DE-627)373755597 (DE-600)2126773-X 15457885 nnns volume:7 year:2009 number:6, p e1000134 https://doi.org/10.1371/journal.pbio.1000134 kostenfrei https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d kostenfrei https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547744/?tool=EBI kostenfrei https://doaj.org/toc/1544-9173 Journal toc kostenfrei https://doaj.org/toc/1545-7885 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 6, p e1000134
allfieldsGer	10.1371/journal.pbio.1000134 doi (DE-627)DOAJ061974323 (DE-599)DOAJ5e8bdd4a44c841e89f6e26ba4767285d DE-627 ger DE-627 rakwb eng QH301-705.5 Pedro Beltrao verfasserin aut Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity. Biology (General) Jonathan C Trinidad verfasserin aut Dorothea Fiedler verfasserin aut Assen Roguev verfasserin aut Wendell A Lim verfasserin aut Kevan M Shokat verfasserin aut Alma L Burlingame verfasserin aut Nevan J Krogan verfasserin aut In PLoS Biology Public Library of Science (PLoS), 2003 7(2009), 6, p e1000134 (DE-627)373755597 (DE-600)2126773-X 15457885 nnns volume:7 year:2009 number:6, p e1000134 https://doi.org/10.1371/journal.pbio.1000134 kostenfrei https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d kostenfrei https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547744/?tool=EBI kostenfrei https://doaj.org/toc/1544-9173 Journal toc kostenfrei https://doaj.org/toc/1545-7885 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 6, p e1000134
allfieldsSound	10.1371/journal.pbio.1000134 doi (DE-627)DOAJ061974323 (DE-599)DOAJ5e8bdd4a44c841e89f6e26ba4767285d DE-627 ger DE-627 rakwb eng QH301-705.5 Pedro Beltrao verfasserin aut Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity. Biology (General) Jonathan C Trinidad verfasserin aut Dorothea Fiedler verfasserin aut Assen Roguev verfasserin aut Wendell A Lim verfasserin aut Kevan M Shokat verfasserin aut Alma L Burlingame verfasserin aut Nevan J Krogan verfasserin aut In PLoS Biology Public Library of Science (PLoS), 2003 7(2009), 6, p e1000134 (DE-627)373755597 (DE-600)2126773-X 15457885 nnns volume:7 year:2009 number:6, p e1000134 https://doi.org/10.1371/journal.pbio.1000134 kostenfrei https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d kostenfrei https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547744/?tool=EBI kostenfrei https://doaj.org/toc/1544-9173 Journal toc kostenfrei https://doaj.org/toc/1545-7885 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2009 6, p e1000134
language	English
source	In PLoS Biology 7(2009), 6, p e1000134 volume:7 year:2009 number:6, p e1000134
sourceStr	In PLoS Biology 7(2009), 6, p e1000134 volume:7 year:2009 number:6, p e1000134
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biology (General)
isfreeaccess_bool	true
container_title	PLoS Biology
authorswithroles_txt_mv	Pedro Beltrao @@aut@@ Jonathan C Trinidad @@aut@@ Dorothea Fiedler @@aut@@ Assen Roguev @@aut@@ Wendell A Lim @@aut@@ Kevan M Shokat @@aut@@ Alma L Burlingame @@aut@@ Nevan J Krogan @@aut@@
publishDateDaySort_date	2009-01-01T00:00:00Z
hierarchy_top_id	373755597
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author	Pedro Beltrao
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topic_title	QH301-705.5 Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species
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title	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
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title_full	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species
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title_sort	evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species
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title_auth	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
abstract	The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity.
abstractGer	The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity.
abstract_unstemmed	The extent by which different cellular components generate phenotypic diversity is an ongoing debate in evolutionary biology that is yet to be addressed by quantitative comparative studies. We conducted an in vivo mass-spectrometry study of the phosphoproteomes of three yeast species (Saccharomyces cerevisiae, Candida albicans, and Schizosaccharomyces pombe) in order to quantify the evolutionary rate of change of phosphorylation. We estimate that kinase-substrate interactions change, at most, two orders of magnitude more slowly than transcription factor (TF)-promoter interactions. Our computational analysis linking kinases to putative substrates recapitulates known phosphoregulation events and provides putative evolutionary histories for the kinase regulation of protein complexes across 11 yeast species. To validate these trends, we used the E-MAP approach to analyze over 2,000 quantitative genetic interactions in S. cerevisiae and Sc. pombe, which demonstrated that protein kinases, and to a greater extent TFs, show lower than average conservation of genetic interactions. We propose therefore that protein kinases are an important source of phenotypic diversity.
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title_short	Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
url	https://doi.org/10.1371/journal.pbio.1000134 https://doaj.org/article/5e8bdd4a44c841e89f6e26ba4767285d https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19547744/?tool=EBI https://doaj.org/toc/1544-9173 https://doaj.org/toc/1545-7885
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Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

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