The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells

Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-te...
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Autor*in:	Xican Li [verfasserIn] Hong Xie [verfasserIn] Qian Jiang [verfasserIn] Gang Wei [verfasserIn] Lishan Lin [verfasserIn] Changying Li [verfasserIn] Xingmei Ou [verfasserIn] Lichan Yang [verfasserIn] Yulu Xie [verfasserIn] Zhen Fu [verfasserIn] Yamei Liu [verfasserIn] Dongfeng Chen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	(+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding

Übergeordnetes Werk:	In: Cellular & Molecular Biology Letters - BMC, 2018, 22(2017), 1, Seite 11
Übergeordnetes Werk:	volume:22 ; year:2017 ; number:1 ; pages:11

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1186/s11658-017-0066-9

Katalog-ID:	DOAJ062140434

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520			\|a Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+.
650		4	\|a (+) Taxifolin; bmMSCs
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allfields	10.1186/s11658-017-0066-9 doi (DE-627)DOAJ062140434 (DE-599)DOAJ62a05b27ee434477a6ecd3e86ea3f4f4 DE-627 ger DE-627 rakwb eng QH573-671 Xican Li verfasserin aut The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+. (+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding Cytology Hong Xie verfasserin aut Qian Jiang verfasserin aut Gang Wei verfasserin aut Lishan Lin verfasserin aut Changying Li verfasserin aut Xingmei Ou verfasserin aut Lichan Yang verfasserin aut Yulu Xie verfasserin aut Zhen Fu verfasserin aut Yamei Liu verfasserin aut Dongfeng Chen verfasserin aut In Cellular & Molecular Biology Letters BMC, 2018 22(2017), 1, Seite 11 (DE-627)363772340 (DE-600)2108724-6 16891392 nnns volume:22 year:2017 number:1 pages:11 https://doi.org/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/article/62a05b27ee434477a6ecd3e86ea3f4f4 kostenfrei http://link.springer.com/article/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/toc/1425-8153 Journal toc kostenfrei https://doaj.org/toc/1689-1392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2017 1 11
spelling	10.1186/s11658-017-0066-9 doi (DE-627)DOAJ062140434 (DE-599)DOAJ62a05b27ee434477a6ecd3e86ea3f4f4 DE-627 ger DE-627 rakwb eng QH573-671 Xican Li verfasserin aut The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+. (+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding Cytology Hong Xie verfasserin aut Qian Jiang verfasserin aut Gang Wei verfasserin aut Lishan Lin verfasserin aut Changying Li verfasserin aut Xingmei Ou verfasserin aut Lichan Yang verfasserin aut Yulu Xie verfasserin aut Zhen Fu verfasserin aut Yamei Liu verfasserin aut Dongfeng Chen verfasserin aut In Cellular & Molecular Biology Letters BMC, 2018 22(2017), 1, Seite 11 (DE-627)363772340 (DE-600)2108724-6 16891392 nnns volume:22 year:2017 number:1 pages:11 https://doi.org/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/article/62a05b27ee434477a6ecd3e86ea3f4f4 kostenfrei http://link.springer.com/article/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/toc/1425-8153 Journal toc kostenfrei https://doaj.org/toc/1689-1392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2017 1 11
allfields_unstemmed	10.1186/s11658-017-0066-9 doi (DE-627)DOAJ062140434 (DE-599)DOAJ62a05b27ee434477a6ecd3e86ea3f4f4 DE-627 ger DE-627 rakwb eng QH573-671 Xican Li verfasserin aut The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+. (+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding Cytology Hong Xie verfasserin aut Qian Jiang verfasserin aut Gang Wei verfasserin aut Lishan Lin verfasserin aut Changying Li verfasserin aut Xingmei Ou verfasserin aut Lichan Yang verfasserin aut Yulu Xie verfasserin aut Zhen Fu verfasserin aut Yamei Liu verfasserin aut Dongfeng Chen verfasserin aut In Cellular & Molecular Biology Letters BMC, 2018 22(2017), 1, Seite 11 (DE-627)363772340 (DE-600)2108724-6 16891392 nnns volume:22 year:2017 number:1 pages:11 https://doi.org/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/article/62a05b27ee434477a6ecd3e86ea3f4f4 kostenfrei http://link.springer.com/article/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/toc/1425-8153 Journal toc kostenfrei https://doaj.org/toc/1689-1392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2017 1 11
allfieldsGer	10.1186/s11658-017-0066-9 doi (DE-627)DOAJ062140434 (DE-599)DOAJ62a05b27ee434477a6ecd3e86ea3f4f4 DE-627 ger DE-627 rakwb eng QH573-671 Xican Li verfasserin aut The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+. (+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding Cytology Hong Xie verfasserin aut Qian Jiang verfasserin aut Gang Wei verfasserin aut Lishan Lin verfasserin aut Changying Li verfasserin aut Xingmei Ou verfasserin aut Lichan Yang verfasserin aut Yulu Xie verfasserin aut Zhen Fu verfasserin aut Yamei Liu verfasserin aut Dongfeng Chen verfasserin aut In Cellular & Molecular Biology Letters BMC, 2018 22(2017), 1, Seite 11 (DE-627)363772340 (DE-600)2108724-6 16891392 nnns volume:22 year:2017 number:1 pages:11 https://doi.org/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/article/62a05b27ee434477a6ecd3e86ea3f4f4 kostenfrei http://link.springer.com/article/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/toc/1425-8153 Journal toc kostenfrei https://doaj.org/toc/1689-1392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2017 1 11
allfieldsSound	10.1186/s11658-017-0066-9 doi (DE-627)DOAJ062140434 (DE-599)DOAJ62a05b27ee434477a6ecd3e86ea3f4f4 DE-627 ger DE-627 rakwb eng QH573-671 Xican Li verfasserin aut The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+. (+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding Cytology Hong Xie verfasserin aut Qian Jiang verfasserin aut Gang Wei verfasserin aut Lishan Lin verfasserin aut Changying Li verfasserin aut Xingmei Ou verfasserin aut Lichan Yang verfasserin aut Yulu Xie verfasserin aut Zhen Fu verfasserin aut Yamei Liu verfasserin aut Dongfeng Chen verfasserin aut In Cellular & Molecular Biology Letters BMC, 2018 22(2017), 1, Seite 11 (DE-627)363772340 (DE-600)2108724-6 16891392 nnns volume:22 year:2017 number:1 pages:11 https://doi.org/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/article/62a05b27ee434477a6ecd3e86ea3f4f4 kostenfrei http://link.springer.com/article/10.1186/s11658-017-0066-9 kostenfrei https://doaj.org/toc/1425-8153 Journal toc kostenfrei https://doaj.org/toc/1689-1392 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2017 1 11
language	English
source	In Cellular & Molecular Biology Letters 22(2017), 1, Seite 11 volume:22 year:2017 number:1 pages:11
sourceStr	In Cellular & Molecular Biology Letters 22(2017), 1, Seite 11 volume:22 year:2017 number:1 pages:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	(+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding Cytology
isfreeaccess_bool	true
container_title	Cellular & Molecular Biology Letters
authorswithroles_txt_mv	Xican Li @@aut@@ Hong Xie @@aut@@ Qian Jiang @@aut@@ Gang Wei @@aut@@ Lishan Lin @@aut@@ Changying Li @@aut@@ Xingmei Ou @@aut@@ Lichan Yang @@aut@@ Yulu Xie @@aut@@ Zhen Fu @@aut@@ Yamei Liu @@aut@@ Dongfeng Chen @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	363772340
id	DOAJ062140434
language_de	englisch
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callnumber-first	Q - Science
author	Xican Li
spellingShingle	Xican Li misc QH573-671 misc (+) Taxifolin; bmMSCs misc •OH damage misc Antioxidant mechanism misc Electron transfer misc Fe2+ binding misc Cytology The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells
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topic_title	QH573-671 The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells (+) Taxifolin; bmMSCs •OH damage Antioxidant mechanism Electron transfer Fe2+ binding
topic	misc QH573-671 misc (+) Taxifolin; bmMSCs misc •OH damage misc Antioxidant mechanism misc Electron transfer misc Fe2+ binding misc Cytology
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title	The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells
ctrlnum	(DE-627)DOAJ062140434 (DE-599)DOAJ62a05b27ee434477a6ecd3e86ea3f4f4
title_full	The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells
author_sort	Xican Li
journal	Cellular & Molecular Biology Letters
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author_browse	Xican Li Hong Xie Qian Jiang Gang Wei Lishan Lin Changying Li Xingmei Ou Lichan Yang Yulu Xie Zhen Fu Yamei Liu Dongfeng Chen
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title_sort	mechanism of (+) taxifolin’s protective antioxidant effect for •oh-treated bone marrow-derived mesenchymal stem cells
callnumber	QH573-671
title_auth	The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells
abstract	Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+.
abstractGer	Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+.
abstract_unstemmed	Abstract The natural dihydroflavonol (+) taxifolin was investigated for its protective effect on Fenton reagent-treated bone marrow-derived mesenchymal stem cells (bmMSCs). Various antioxidant assays were used to determine the possible mechanism. These included •OH-scavenging, 2-phenyl-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging (PTIO•-scavenging), 1, 1-diphenyl-2-picryl-hydrazl radical-scavenging (DPPH•-scavenging), 2, 2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical-scavenging (ABTS+•-scavenging), Fe3+-reducing, and Cu2+-reducing assays. The Fe2+-binding reaction was also investigated using UV-Vis spectra. The results revealed that cell viability was fully restored, even increasing to 142.9 ± 9.3% after treatment with (+) taxifolin. In the antioxidant assays, (+) taxifolin was observed to efficiently scavenge •OH, DPPH• and ABTS+• radicals, and to increase the relative Cu2+- and Fe3+-reducing levels. In the PTIO•-scavenging assay, its IC50 values varied with pH. In the Fe2+-binding reaction, (+) taxifolin was found to yield a green solution with two UV-Vis absorbance peaks: λmax = 433 nm (ε =5.2 × 102 L mol−1 cm −1) and λmax = 721 nm (ε = 5.1 × 102 L mol−1 cm −1). These results indicate that (+) taxifolin can act as an effective •OH-scavenger, protecting bmMSCs from •OH-induced damage. Its •OH-scavenging action consists of direct and indirect antioxidant effects. Direct antioxidation occurs via multiple pathways, including ET, PCET or HAT. Indirect antioxidation involves binding to Fe2+.
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title_short	The mechanism of (+) taxifolin’s protective antioxidant effect for •OH-treated bone marrow-derived mesenchymal stem cells
url	https://doi.org/10.1186/s11658-017-0066-9 https://doaj.org/article/62a05b27ee434477a6ecd3e86ea3f4f4 http://link.springer.com/article/10.1186/s11658-017-0066-9 https://doaj.org/toc/1425-8153 https://doaj.org/toc/1689-1392
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author2	Hong Xie Qian Jiang Gang Wei Lishan Lin Changying Li Xingmei Ou Lichan Yang Yulu Xie Zhen Fu Yamei Liu Dongfeng Chen
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