Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy

In recent years autophagy has attracted the attention of researchers from many medical fields, including cancer research, and certain anti-macroautophagy drugs in combination with cytotoxic or targeted therapies have entered clinical trials. In the present study, we focused on a less explored subtyp...
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Autor*in:	Tereza Losmanova [verfasserIn] Philipp Zens [verfasserIn] Amina Scherz [verfasserIn] Ralph A. Schmid [verfasserIn] Mario P. Tschan [verfasserIn] Sabina Berezowska [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	lung cancer non-small cell lung cancer neoadjuvant therapy chaperone-mediated autophagy (CMA) LAMP2A HSC70

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 10(2021), 10, p 2731
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:10, p 2731

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells10102731

Katalog-ID:	DOAJ062253115

Internformat


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allfieldsGer	10.3390/cells10102731 doi (DE-627)DOAJ062253115 (DE-599)DOAJca70d4fc0f984b81bfdb1c5f54e109fc DE-627 ger DE-627 rakwb eng QH573-671 Tereza Losmanova verfasserin aut Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In recent years autophagy has attracted the attention of researchers from many medical fields, including cancer research, and certain anti-macroautophagy drugs in combination with cytotoxic or targeted therapies have entered clinical trials. In the present study, we focused on a less explored subtype of autophagy, i.e., chaperone-mediated autophagy (CMA), with the key proteins LAMP2A and HSPA8 (HSC70), and their immunohistochemical evaluation with previously extensively validated antibodies. We were interested in whether the marker expression is influenced by the antecedent therapy, and its correlation with survival on a cohort of patients with non-small cell lung cancer (NSCLC) after neoadjuvant therapy and matched primary resected tumors. In concordance with our previous study, we did not find any intratumoral heterogeneity, nor correlation between the two parameters, nor correlation between the markers and any included pathological parameters. Surprisingly, the expression of both markers was also independent to tumor response or administered neoadjuvant treatment. In the survival analysis, the results were only significant for LAMP2A, where higher levels were associated with longer 5-year overall survival and disease-free survival for the mixed group of adenocarcinomas and squamous cell carcinomas (<i<p</i< < 0.0001 and <i<p</i< = 0.0019 respectively) as well as the squamous cell carcinoma subgroup (<i<p</i< = 0.0001 and <i<p</i< = 0.0001 respectively). LAMP2A was also an independent prognostic marker in univariate and multivariate analysis. lung cancer non-small cell lung cancer neoadjuvant therapy chaperone-mediated autophagy (CMA) LAMP2A HSC70 Cytology Philipp Zens verfasserin aut Amina Scherz verfasserin aut Ralph A. Schmid verfasserin aut Mario P. Tschan verfasserin aut Sabina Berezowska verfasserin aut In Cells MDPI AG, 2012 10(2021), 10, p 2731 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:10, p 2731 https://doi.org/10.3390/cells10102731 kostenfrei https://doaj.org/article/ca70d4fc0f984b81bfdb1c5f54e109fc kostenfrei https://www.mdpi.com/2073-4409/10/10/2731 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 10, p 2731
allfieldsSound	10.3390/cells10102731 doi (DE-627)DOAJ062253115 (DE-599)DOAJca70d4fc0f984b81bfdb1c5f54e109fc DE-627 ger DE-627 rakwb eng QH573-671 Tereza Losmanova verfasserin aut Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In recent years autophagy has attracted the attention of researchers from many medical fields, including cancer research, and certain anti-macroautophagy drugs in combination with cytotoxic or targeted therapies have entered clinical trials. In the present study, we focused on a less explored subtype of autophagy, i.e., chaperone-mediated autophagy (CMA), with the key proteins LAMP2A and HSPA8 (HSC70), and their immunohistochemical evaluation with previously extensively validated antibodies. We were interested in whether the marker expression is influenced by the antecedent therapy, and its correlation with survival on a cohort of patients with non-small cell lung cancer (NSCLC) after neoadjuvant therapy and matched primary resected tumors. In concordance with our previous study, we did not find any intratumoral heterogeneity, nor correlation between the two parameters, nor correlation between the markers and any included pathological parameters. Surprisingly, the expression of both markers was also independent to tumor response or administered neoadjuvant treatment. In the survival analysis, the results were only significant for LAMP2A, where higher levels were associated with longer 5-year overall survival and disease-free survival for the mixed group of adenocarcinomas and squamous cell carcinomas (<i<p</i< < 0.0001 and <i<p</i< = 0.0019 respectively) as well as the squamous cell carcinoma subgroup (<i<p</i< = 0.0001 and <i<p</i< = 0.0001 respectively). LAMP2A was also an independent prognostic marker in univariate and multivariate analysis. lung cancer non-small cell lung cancer neoadjuvant therapy chaperone-mediated autophagy (CMA) LAMP2A HSC70 Cytology Philipp Zens verfasserin aut Amina Scherz verfasserin aut Ralph A. Schmid verfasserin aut Mario P. Tschan verfasserin aut Sabina Berezowska verfasserin aut In Cells MDPI AG, 2012 10(2021), 10, p 2731 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:10, p 2731 https://doi.org/10.3390/cells10102731 kostenfrei https://doaj.org/article/ca70d4fc0f984b81bfdb1c5f54e109fc kostenfrei https://www.mdpi.com/2073-4409/10/10/2731 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 10, p 2731
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callnumber-first	Q - Science
author	Tereza Losmanova
spellingShingle	Tereza Losmanova misc QH573-671 misc lung cancer misc non-small cell lung cancer misc neoadjuvant therapy misc chaperone-mediated autophagy (CMA) misc LAMP2A misc HSC70 misc Cytology Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy
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topic_title	QH573-671 Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy lung cancer non-small cell lung cancer neoadjuvant therapy chaperone-mediated autophagy (CMA) LAMP2A HSC70
topic	misc QH573-671 misc lung cancer misc non-small cell lung cancer misc neoadjuvant therapy misc chaperone-mediated autophagy (CMA) misc LAMP2A misc HSC70 misc Cytology
topic_unstemmed	misc QH573-671 misc lung cancer misc non-small cell lung cancer misc neoadjuvant therapy misc chaperone-mediated autophagy (CMA) misc LAMP2A misc HSC70 misc Cytology
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title	Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy
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title_full	Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy
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author_browse	Tereza Losmanova Philipp Zens Amina Scherz Ralph A. Schmid Mario P. Tschan Sabina Berezowska
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title_sort	chaperone-mediated autophagy markers lamp2a and hspa8 in advanced non-small cell lung cancer after neoadjuvant therapy
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title_auth	Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy
abstract	In recent years autophagy has attracted the attention of researchers from many medical fields, including cancer research, and certain anti-macroautophagy drugs in combination with cytotoxic or targeted therapies have entered clinical trials. In the present study, we focused on a less explored subtype of autophagy, i.e., chaperone-mediated autophagy (CMA), with the key proteins LAMP2A and HSPA8 (HSC70), and their immunohistochemical evaluation with previously extensively validated antibodies. We were interested in whether the marker expression is influenced by the antecedent therapy, and its correlation with survival on a cohort of patients with non-small cell lung cancer (NSCLC) after neoadjuvant therapy and matched primary resected tumors. In concordance with our previous study, we did not find any intratumoral heterogeneity, nor correlation between the two parameters, nor correlation between the markers and any included pathological parameters. Surprisingly, the expression of both markers was also independent to tumor response or administered neoadjuvant treatment. In the survival analysis, the results were only significant for LAMP2A, where higher levels were associated with longer 5-year overall survival and disease-free survival for the mixed group of adenocarcinomas and squamous cell carcinomas (<i<p</i< < 0.0001 and <i<p</i< = 0.0019 respectively) as well as the squamous cell carcinoma subgroup (<i<p</i< = 0.0001 and <i<p</i< = 0.0001 respectively). LAMP2A was also an independent prognostic marker in univariate and multivariate analysis.
abstractGer	In recent years autophagy has attracted the attention of researchers from many medical fields, including cancer research, and certain anti-macroautophagy drugs in combination with cytotoxic or targeted therapies have entered clinical trials. In the present study, we focused on a less explored subtype of autophagy, i.e., chaperone-mediated autophagy (CMA), with the key proteins LAMP2A and HSPA8 (HSC70), and their immunohistochemical evaluation with previously extensively validated antibodies. We were interested in whether the marker expression is influenced by the antecedent therapy, and its correlation with survival on a cohort of patients with non-small cell lung cancer (NSCLC) after neoadjuvant therapy and matched primary resected tumors. In concordance with our previous study, we did not find any intratumoral heterogeneity, nor correlation between the two parameters, nor correlation between the markers and any included pathological parameters. Surprisingly, the expression of both markers was also independent to tumor response or administered neoadjuvant treatment. In the survival analysis, the results were only significant for LAMP2A, where higher levels were associated with longer 5-year overall survival and disease-free survival for the mixed group of adenocarcinomas and squamous cell carcinomas (<i<p</i< < 0.0001 and <i<p</i< = 0.0019 respectively) as well as the squamous cell carcinoma subgroup (<i<p</i< = 0.0001 and <i<p</i< = 0.0001 respectively). LAMP2A was also an independent prognostic marker in univariate and multivariate analysis.
abstract_unstemmed	In recent years autophagy has attracted the attention of researchers from many medical fields, including cancer research, and certain anti-macroautophagy drugs in combination with cytotoxic or targeted therapies have entered clinical trials. In the present study, we focused on a less explored subtype of autophagy, i.e., chaperone-mediated autophagy (CMA), with the key proteins LAMP2A and HSPA8 (HSC70), and their immunohistochemical evaluation with previously extensively validated antibodies. We were interested in whether the marker expression is influenced by the antecedent therapy, and its correlation with survival on a cohort of patients with non-small cell lung cancer (NSCLC) after neoadjuvant therapy and matched primary resected tumors. In concordance with our previous study, we did not find any intratumoral heterogeneity, nor correlation between the two parameters, nor correlation between the markers and any included pathological parameters. Surprisingly, the expression of both markers was also independent to tumor response or administered neoadjuvant treatment. In the survival analysis, the results were only significant for LAMP2A, where higher levels were associated with longer 5-year overall survival and disease-free survival for the mixed group of adenocarcinomas and squamous cell carcinomas (<i<p</i< < 0.0001 and <i<p</i< = 0.0019 respectively) as well as the squamous cell carcinoma subgroup (<i<p</i< = 0.0001 and <i<p</i< = 0.0001 respectively). LAMP2A was also an independent prognostic marker in univariate and multivariate analysis.
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title_short	Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy
url	https://doi.org/10.3390/cells10102731 https://doaj.org/article/ca70d4fc0f984b81bfdb1c5f54e109fc https://www.mdpi.com/2073-4409/10/10/2731 https://doaj.org/toc/2073-4409
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up_date	2024-07-04T00:54:31.370Z
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Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy

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