Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic

Alginate is a polysaccharide used extensively in biomedical applications due to its biocompatibility and suitability for hydrogel fabrication using mild reaction chemistries. Though alginate has commonly been crosslinked using divalent cations, covalent crosslinking chemistries have also been develo...
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Gespeichert in:

Autor*in:	Sara Trujillo [verfasserIn] Melanie Seow [verfasserIn] Aline Lueckgen [verfasserIn] Manuel Salmeron-Sanchez [verfasserIn] Amaia Cipitria [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	alginate hydrogel fibronectin enzymatic degradation dual crosslinking covalent and ionic crosslinking dynamic mechanical properties

Übergeordnetes Werk:	In: Polymers - MDPI AG, 2011, 13(2021), 3, p 433
Übergeordnetes Werk:	volume:13 ; year:2021 ; number:3, p 433

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/polym13030433

Katalog-ID:	DOAJ062634917

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authorswithroles_txt_mv	Sara Trujillo @@aut@@ Melanie Seow @@aut@@ Aline Lueckgen @@aut@@ Manuel Salmeron-Sanchez @@aut@@ Amaia Cipitria @@aut@@
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callnumber-first	Q - Science
author	Sara Trujillo
spellingShingle	Sara Trujillo misc QD241-441 misc alginate hydrogel misc fibronectin misc enzymatic degradation misc dual crosslinking misc covalent and ionic crosslinking misc dynamic mechanical properties misc Organic chemistry Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic
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topic_title	QD241-441 Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic alginate hydrogel fibronectin enzymatic degradation dual crosslinking covalent and ionic crosslinking dynamic mechanical properties
topic	misc QD241-441 misc alginate hydrogel misc fibronectin misc enzymatic degradation misc dual crosslinking misc covalent and ionic crosslinking misc dynamic mechanical properties misc Organic chemistry
topic_unstemmed	misc QD241-441 misc alginate hydrogel misc fibronectin misc enzymatic degradation misc dual crosslinking misc covalent and ionic crosslinking misc dynamic mechanical properties misc Organic chemistry
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title	Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic
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title_full	Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic
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title_sort	dynamic mechanical control of alginate-fibronectin hydrogels with dual crosslinking: covalent and ionic
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title_auth	Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic
abstract	Alginate is a polysaccharide used extensively in biomedical applications due to its biocompatibility and suitability for hydrogel fabrication using mild reaction chemistries. Though alginate has commonly been crosslinked using divalent cations, covalent crosslinking chemistries have also been developed. Hydrogels with tuneable mechanical properties are required for many biomedical applications to mimic the stiffness of different tissues. Here, we present a strategy to engineer alginate hydrogels with tuneable mechanical properties by covalent crosslinking of a norbornene-modified alginate using ultraviolet (UV)-initiated thiol-ene chemistry. We also demonstrate that the system can be functionalised with cues such as full-length fibronectin and protease-degradable sequences. Finally, we take advantage of alginate’s ability to be crosslinked covalently and ionically to design dual crosslinked constructs enabling dynamic control of mechanical properties, with gels that undergo cycles of stiffening–softening by adding and quenching calcium cations. Overall, we present a versatile hydrogel with tuneable and dynamic mechanical properties, and incorporate cell-interactive features such as cell-mediated protease-induced degradability and full-length proteins, which may find applications in a variety of biomedical contexts.
abstractGer	Alginate is a polysaccharide used extensively in biomedical applications due to its biocompatibility and suitability for hydrogel fabrication using mild reaction chemistries. Though alginate has commonly been crosslinked using divalent cations, covalent crosslinking chemistries have also been developed. Hydrogels with tuneable mechanical properties are required for many biomedical applications to mimic the stiffness of different tissues. Here, we present a strategy to engineer alginate hydrogels with tuneable mechanical properties by covalent crosslinking of a norbornene-modified alginate using ultraviolet (UV)-initiated thiol-ene chemistry. We also demonstrate that the system can be functionalised with cues such as full-length fibronectin and protease-degradable sequences. Finally, we take advantage of alginate’s ability to be crosslinked covalently and ionically to design dual crosslinked constructs enabling dynamic control of mechanical properties, with gels that undergo cycles of stiffening–softening by adding and quenching calcium cations. Overall, we present a versatile hydrogel with tuneable and dynamic mechanical properties, and incorporate cell-interactive features such as cell-mediated protease-induced degradability and full-length proteins, which may find applications in a variety of biomedical contexts.
abstract_unstemmed	Alginate is a polysaccharide used extensively in biomedical applications due to its biocompatibility and suitability for hydrogel fabrication using mild reaction chemistries. Though alginate has commonly been crosslinked using divalent cations, covalent crosslinking chemistries have also been developed. Hydrogels with tuneable mechanical properties are required for many biomedical applications to mimic the stiffness of different tissues. Here, we present a strategy to engineer alginate hydrogels with tuneable mechanical properties by covalent crosslinking of a norbornene-modified alginate using ultraviolet (UV)-initiated thiol-ene chemistry. We also demonstrate that the system can be functionalised with cues such as full-length fibronectin and protease-degradable sequences. Finally, we take advantage of alginate’s ability to be crosslinked covalently and ionically to design dual crosslinked constructs enabling dynamic control of mechanical properties, with gels that undergo cycles of stiffening–softening by adding and quenching calcium cations. Overall, we present a versatile hydrogel with tuneable and dynamic mechanical properties, and incorporate cell-interactive features such as cell-mediated protease-induced degradability and full-length proteins, which may find applications in a variety of biomedical contexts.
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container_issue	3, p 433
title_short	Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic
url	https://doi.org/10.3390/polym13030433 https://doaj.org/article/19d055bbd33d484586bf11055a3608eb https://www.mdpi.com/2073-4360/13/3/433 https://doaj.org/toc/2073-4360
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up_date	2024-07-04T02:22:11.930Z
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Dynamic Mechanical Control of Alginate-Fibronectin Hydrogels with Dual Crosslinking: Covalent and Ionic

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