Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering

Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based se...
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Autor*in:	Zahra Rashidbenam [verfasserIn] Mohd Hafidzul Jasman [verfasserIn] Guan Hee Tan [verfasserIn] Eng Hong Goh [verfasserIn] Xeng Inn Fam [verfasserIn] Christopher Chee Kong Ho [verfasserIn] Zulkifli Md Zainuddin [verfasserIn] Reynu Rajan [verfasserIn] Rizal Abdul Rani [verfasserIn] Fatimah Mohd Nor [verfasserIn] Mohamad Aznan Shuhaili [verfasserIn] Nik Ritza Kosai [verfasserIn] Farrah Hani Imran [verfasserIn] Min Hwei Ng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 22(2021), 7, p 3350
Übergeordnetes Werk:	volume:22 ; year:2021 ; number:7, p 3350

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms22073350

Katalog-ID:	DOAJ062712845

Internformat


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520			\|a Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries.
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allfields	10.3390/ijms22073350 doi (DE-627)DOAJ062712845 (DE-599)DOAJa73472c2dadf4ca1a3e9d67d7f868010 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Zahra Rashidbenam verfasserin aut Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries. adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft Biology (General) Chemistry Mohd Hafidzul Jasman verfasserin aut Guan Hee Tan verfasserin aut Eng Hong Goh verfasserin aut Xeng Inn Fam verfasserin aut Christopher Chee Kong Ho verfasserin aut Zulkifli Md Zainuddin verfasserin aut Reynu Rajan verfasserin aut Rizal Abdul Rani verfasserin aut Fatimah Mohd Nor verfasserin aut Mohamad Aznan Shuhaili verfasserin aut Nik Ritza Kosai verfasserin aut Farrah Hani Imran verfasserin aut Min Hwei Ng verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 7, p 3350 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:7, p 3350 https://doi.org/10.3390/ijms22073350 kostenfrei https://doaj.org/article/a73472c2dadf4ca1a3e9d67d7f868010 kostenfrei https://www.mdpi.com/1422-0067/22/7/3350 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 7, p 3350
spelling	10.3390/ijms22073350 doi (DE-627)DOAJ062712845 (DE-599)DOAJa73472c2dadf4ca1a3e9d67d7f868010 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Zahra Rashidbenam verfasserin aut Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries. adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft Biology (General) Chemistry Mohd Hafidzul Jasman verfasserin aut Guan Hee Tan verfasserin aut Eng Hong Goh verfasserin aut Xeng Inn Fam verfasserin aut Christopher Chee Kong Ho verfasserin aut Zulkifli Md Zainuddin verfasserin aut Reynu Rajan verfasserin aut Rizal Abdul Rani verfasserin aut Fatimah Mohd Nor verfasserin aut Mohamad Aznan Shuhaili verfasserin aut Nik Ritza Kosai verfasserin aut Farrah Hani Imran verfasserin aut Min Hwei Ng verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 7, p 3350 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:7, p 3350 https://doi.org/10.3390/ijms22073350 kostenfrei https://doaj.org/article/a73472c2dadf4ca1a3e9d67d7f868010 kostenfrei https://www.mdpi.com/1422-0067/22/7/3350 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 7, p 3350
allfields_unstemmed	10.3390/ijms22073350 doi (DE-627)DOAJ062712845 (DE-599)DOAJa73472c2dadf4ca1a3e9d67d7f868010 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Zahra Rashidbenam verfasserin aut Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries. adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft Biology (General) Chemistry Mohd Hafidzul Jasman verfasserin aut Guan Hee Tan verfasserin aut Eng Hong Goh verfasserin aut Xeng Inn Fam verfasserin aut Christopher Chee Kong Ho verfasserin aut Zulkifli Md Zainuddin verfasserin aut Reynu Rajan verfasserin aut Rizal Abdul Rani verfasserin aut Fatimah Mohd Nor verfasserin aut Mohamad Aznan Shuhaili verfasserin aut Nik Ritza Kosai verfasserin aut Farrah Hani Imran verfasserin aut Min Hwei Ng verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 7, p 3350 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:7, p 3350 https://doi.org/10.3390/ijms22073350 kostenfrei https://doaj.org/article/a73472c2dadf4ca1a3e9d67d7f868010 kostenfrei https://www.mdpi.com/1422-0067/22/7/3350 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 7, p 3350
allfieldsGer	10.3390/ijms22073350 doi (DE-627)DOAJ062712845 (DE-599)DOAJa73472c2dadf4ca1a3e9d67d7f868010 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Zahra Rashidbenam verfasserin aut Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries. adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft Biology (General) Chemistry Mohd Hafidzul Jasman verfasserin aut Guan Hee Tan verfasserin aut Eng Hong Goh verfasserin aut Xeng Inn Fam verfasserin aut Christopher Chee Kong Ho verfasserin aut Zulkifli Md Zainuddin verfasserin aut Reynu Rajan verfasserin aut Rizal Abdul Rani verfasserin aut Fatimah Mohd Nor verfasserin aut Mohamad Aznan Shuhaili verfasserin aut Nik Ritza Kosai verfasserin aut Farrah Hani Imran verfasserin aut Min Hwei Ng verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 7, p 3350 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:7, p 3350 https://doi.org/10.3390/ijms22073350 kostenfrei https://doaj.org/article/a73472c2dadf4ca1a3e9d67d7f868010 kostenfrei https://www.mdpi.com/1422-0067/22/7/3350 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 7, p 3350
allfieldsSound	10.3390/ijms22073350 doi (DE-627)DOAJ062712845 (DE-599)DOAJa73472c2dadf4ca1a3e9d67d7f868010 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Zahra Rashidbenam verfasserin aut Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries. adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft Biology (General) Chemistry Mohd Hafidzul Jasman verfasserin aut Guan Hee Tan verfasserin aut Eng Hong Goh verfasserin aut Xeng Inn Fam verfasserin aut Christopher Chee Kong Ho verfasserin aut Zulkifli Md Zainuddin verfasserin aut Reynu Rajan verfasserin aut Rizal Abdul Rani verfasserin aut Fatimah Mohd Nor verfasserin aut Mohamad Aznan Shuhaili verfasserin aut Nik Ritza Kosai verfasserin aut Farrah Hani Imran verfasserin aut Min Hwei Ng verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 7, p 3350 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:7, p 3350 https://doi.org/10.3390/ijms22073350 kostenfrei https://doaj.org/article/a73472c2dadf4ca1a3e9d67d7f868010 kostenfrei https://www.mdpi.com/1422-0067/22/7/3350 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 7, p 3350
language	English
source	In International Journal of Molecular Sciences 22(2021), 7, p 3350 volume:22 year:2021 number:7, p 3350
sourceStr	In International Journal of Molecular Sciences 22(2021), 7, p 3350 volume:22 year:2021 number:7, p 3350
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Zahra Rashidbenam @@aut@@ Mohd Hafidzul Jasman @@aut@@ Guan Hee Tan @@aut@@ Eng Hong Goh @@aut@@ Xeng Inn Fam @@aut@@ Christopher Chee Kong Ho @@aut@@ Zulkifli Md Zainuddin @@aut@@ Reynu Rajan @@aut@@ Rizal Abdul Rani @@aut@@ Fatimah Mohd Nor @@aut@@ Mohamad Aznan Shuhaili @@aut@@ Nik Ritza Kosai @@aut@@ Farrah Hani Imran @@aut@@ Min Hwei Ng @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ062712845
language_de	englisch
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callnumber-first	Q - Science
author	Zahra Rashidbenam
spellingShingle	Zahra Rashidbenam misc QH301-705.5 misc QD1-999 misc adipose-derived stem cell misc self-assembled scaffold misc extracellular matrix misc hypoxic condition misc tissue engineering misc autologous urethral graft misc Biology (General) misc Chemistry Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering
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topic_title	QH301-705.5 QD1-999 Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering adipose-derived stem cell self-assembled scaffold extracellular matrix hypoxic condition tissue engineering autologous urethral graft
topic	misc QH301-705.5 misc QD1-999 misc adipose-derived stem cell misc self-assembled scaffold misc extracellular matrix misc hypoxic condition misc tissue engineering misc autologous urethral graft misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc adipose-derived stem cell misc self-assembled scaffold misc extracellular matrix misc hypoxic condition misc tissue engineering misc autologous urethral graft misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc adipose-derived stem cell misc self-assembled scaffold misc extracellular matrix misc hypoxic condition misc tissue engineering misc autologous urethral graft misc Biology (General) misc Chemistry
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title	Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering
ctrlnum	(DE-627)DOAJ062712845 (DE-599)DOAJa73472c2dadf4ca1a3e9d67d7f868010
title_full	Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering
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author_browse	Zahra Rashidbenam Mohd Hafidzul Jasman Guan Hee Tan Eng Hong Goh Xeng Inn Fam Christopher Chee Kong Ho Zulkifli Md Zainuddin Reynu Rajan Rizal Abdul Rani Fatimah Mohd Nor Mohamad Aznan Shuhaili Nik Ritza Kosai Farrah Hani Imran Min Hwei Ng
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author-letter	Zahra Rashidbenam
doi_str_mv	10.3390/ijms22073350
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title_sort	fabrication of adipose-derived stem cell-based self-assembled scaffold under hypoxia and mechanical stimulation for urethral tissue engineering
callnumber	QH301-705.5
title_auth	Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering
abstract	Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries.
abstractGer	Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries.
abstract_unstemmed	Long urethral strictures are often treated with autologous genital skin and buccal mucosa grafts; however, risk of hair ingrowth and donor site morbidity, restrict their application. To overcome this, we introduced a tissue-engineered human urethra comprising adipose-derived stem cell (ASC)-based self-assembled scaffold, human urothelial cells (UCs) and smooth muscle cells (SMCs). ASCs were cultured with ascorbic acid to stimulate extracellular matrix (ECM) production. The scaffold (ECM) was stained with collagen type-I antibody and the thickness was measured under a confocal microscope. Results showed that the thickest scaffold (28.06 ± 0.59 μm) was achieved with 3 × 10<sup<4</sup< cells/cm<sup<2</sup< seeding density, 100 μg/mL ascorbic acid concentration under hypoxic and dynamic culture condition. The biocompatibility assessment showed that UCs and SMCs seeded on the scaffold could proliferate and maintain the expression of their markers (CK7, CK20, UPIa, and UPII) and (α-SMA, MHC and Smootheline), respectively, after 14 days of in vitro culture. ECM gene expression analysis showed that the ASC and dermal fibroblast-based scaffolds (control) were comparable. The ASC-based scaffold can be handled and removed from the plate. This suggests that multiple layers of scaffold can be stacked to form the urothelium (seeded with UCs), submucosal layer (ASCs only), and smooth muscle layer (seeded with SMCs) and has the potential to be developed into a fully functional human urethra for urethral reconstructive surgeries.
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container_issue	7, p 3350
title_short	Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering
url	https://doi.org/10.3390/ijms22073350 https://doaj.org/article/a73472c2dadf4ca1a3e9d67d7f868010 https://www.mdpi.com/1422-0067/22/7/3350 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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author2Str	Mohd Hafidzul Jasman Guan Hee Tan Eng Hong Goh Xeng Inn Fam Christopher Chee Kong Ho Zulkifli Md Zainuddin Reynu Rajan Rizal Abdul Rani Fatimah Mohd Nor Mohamad Aznan Shuhaili Nik Ritza Kosai Farrah Hani Imran Min Hwei Ng
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up_date	2024-07-03T13:37:52.826Z
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Fabrication of Adipose-Derived Stem Cell-Based Self-Assembled Scaffold under Hypoxia and Mechanical Stimulation for Urethral Tissue Engineering

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