Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Uchida T [verfasserIn] Kaku Y [verfasserIn] Hayasaka H [verfasserIn] Kofuji M [verfasserIn] Momose N [verfasserIn] Miyazawa H [verfasserIn] Ueda Y [verfasserIn] Ito K [verfasserIn] Ookawara S [verfasserIn] Morishita Y [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate

Übergeordnetes Werk:	In: Medical Devices: Evidence and Research - Dove Medical Press, 2009, (2019), Seite 429-433
Übergeordnetes Werk:	year:2019 ; pages:429-433

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ063119935

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allfields	(DE-627)DOAJ063119935 (DE-599)DOAJb4d0fea4316740078975f9d4b3302dc5 DE-627 ger DE-627 rakwb eng R855-855.5 Uchida T verfasserin aut Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate Medical technology Kaku Y verfasserin aut Hayasaka H verfasserin aut Kofuji M verfasserin aut Momose N verfasserin aut Miyazawa H verfasserin aut Ueda Y verfasserin aut Ito K verfasserin aut Ookawara S verfasserin aut Morishita Y verfasserin aut In Medical Devices: Evidence and Research Dove Medical Press, 2009 (2019), Seite 429-433 (DE-627)611726149 (DE-600)2520731-3 11791470 nnns year:2019 pages:429-433 https://doaj.org/article/b4d0fea4316740078975f9d4b3302dc5 kostenfrei https://www.dovepress.com/utility-of-an-automatic-limulus-amebocyte-lysate-kinetic-turbidimetric-peer-reviewed-article-MDER kostenfrei https://doaj.org/toc/1179-1470 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 429-433
spelling	(DE-627)DOAJ063119935 (DE-599)DOAJb4d0fea4316740078975f9d4b3302dc5 DE-627 ger DE-627 rakwb eng R855-855.5 Uchida T verfasserin aut Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate Medical technology Kaku Y verfasserin aut Hayasaka H verfasserin aut Kofuji M verfasserin aut Momose N verfasserin aut Miyazawa H verfasserin aut Ueda Y verfasserin aut Ito K verfasserin aut Ookawara S verfasserin aut Morishita Y verfasserin aut In Medical Devices: Evidence and Research Dove Medical Press, 2009 (2019), Seite 429-433 (DE-627)611726149 (DE-600)2520731-3 11791470 nnns year:2019 pages:429-433 https://doaj.org/article/b4d0fea4316740078975f9d4b3302dc5 kostenfrei https://www.dovepress.com/utility-of-an-automatic-limulus-amebocyte-lysate-kinetic-turbidimetric-peer-reviewed-article-MDER kostenfrei https://doaj.org/toc/1179-1470 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 429-433
allfields_unstemmed	(DE-627)DOAJ063119935 (DE-599)DOAJb4d0fea4316740078975f9d4b3302dc5 DE-627 ger DE-627 rakwb eng R855-855.5 Uchida T verfasserin aut Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate Medical technology Kaku Y verfasserin aut Hayasaka H verfasserin aut Kofuji M verfasserin aut Momose N verfasserin aut Miyazawa H verfasserin aut Ueda Y verfasserin aut Ito K verfasserin aut Ookawara S verfasserin aut Morishita Y verfasserin aut In Medical Devices: Evidence and Research Dove Medical Press, 2009 (2019), Seite 429-433 (DE-627)611726149 (DE-600)2520731-3 11791470 nnns year:2019 pages:429-433 https://doaj.org/article/b4d0fea4316740078975f9d4b3302dc5 kostenfrei https://www.dovepress.com/utility-of-an-automatic-limulus-amebocyte-lysate-kinetic-turbidimetric-peer-reviewed-article-MDER kostenfrei https://doaj.org/toc/1179-1470 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 429-433
allfieldsGer	(DE-627)DOAJ063119935 (DE-599)DOAJb4d0fea4316740078975f9d4b3302dc5 DE-627 ger DE-627 rakwb eng R855-855.5 Uchida T verfasserin aut Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate Medical technology Kaku Y verfasserin aut Hayasaka H verfasserin aut Kofuji M verfasserin aut Momose N verfasserin aut Miyazawa H verfasserin aut Ueda Y verfasserin aut Ito K verfasserin aut Ookawara S verfasserin aut Morishita Y verfasserin aut In Medical Devices: Evidence and Research Dove Medical Press, 2009 (2019), Seite 429-433 (DE-627)611726149 (DE-600)2520731-3 11791470 nnns year:2019 pages:429-433 https://doaj.org/article/b4d0fea4316740078975f9d4b3302dc5 kostenfrei https://www.dovepress.com/utility-of-an-automatic-limulus-amebocyte-lysate-kinetic-turbidimetric-peer-reviewed-article-MDER kostenfrei https://doaj.org/toc/1179-1470 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 429-433
allfieldsSound	(DE-627)DOAJ063119935 (DE-599)DOAJb4d0fea4316740078975f9d4b3302dc5 DE-627 ger DE-627 rakwb eng R855-855.5 Uchida T verfasserin aut Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate Medical technology Kaku Y verfasserin aut Hayasaka H verfasserin aut Kofuji M verfasserin aut Momose N verfasserin aut Miyazawa H verfasserin aut Ueda Y verfasserin aut Ito K verfasserin aut Ookawara S verfasserin aut Morishita Y verfasserin aut In Medical Devices: Evidence and Research Dove Medical Press, 2009 (2019), Seite 429-433 (DE-627)611726149 (DE-600)2520731-3 11791470 nnns year:2019 pages:429-433 https://doaj.org/article/b4d0fea4316740078975f9d4b3302dc5 kostenfrei https://www.dovepress.com/utility-of-an-automatic-limulus-amebocyte-lysate-kinetic-turbidimetric-peer-reviewed-article-MDER kostenfrei https://doaj.org/toc/1179-1470 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 429-433
language	English
source	In Medical Devices: Evidence and Research (2019), Seite 429-433 year:2019 pages:429-433
sourceStr	In Medical Devices: Evidence and Research (2019), Seite 429-433 year:2019 pages:429-433
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate Medical technology
isfreeaccess_bool	true
container_title	Medical Devices: Evidence and Research
authorswithroles_txt_mv	Uchida T @@aut@@ Kaku Y @@aut@@ Hayasaka H @@aut@@ Kofuji M @@aut@@ Momose N @@aut@@ Miyazawa H @@aut@@ Ueda Y @@aut@@ Ito K @@aut@@ Ookawara S @@aut@@ Morishita Y @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	611726149
id	DOAJ063119935
language_de	englisch
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callnumber-first	R - Medicine
author	Uchida T
spellingShingle	Uchida T misc R855-855.5 misc endotoxin misc dialysate misc hemodialysis misc Limulus amebocyte lysate misc chromogenic substrate misc Medical technology Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples
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topic_title	R855-855.5 Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples endotoxin dialysate hemodialysis Limulus amebocyte lysate chromogenic substrate
topic	misc R855-855.5 misc endotoxin misc dialysate misc hemodialysis misc Limulus amebocyte lysate misc chromogenic substrate misc Medical technology
topic_unstemmed	misc R855-855.5 misc endotoxin misc dialysate misc hemodialysis misc Limulus amebocyte lysate misc chromogenic substrate misc Medical technology
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title	Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples
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title_full	Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples
author_sort	Uchida T
journal	Medical Devices: Evidence and Research
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author_browse	Uchida T Kaku Y Hayasaka H Kofuji M Momose N Miyazawa H Ueda Y Ito K Ookawara S Morishita Y
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title_sort	utility of an automatic limulus amebocyte lysate kinetic turbidimetric test for endotoxin screening of dialysate samples
callnumber	R855-855.5
title_auth	Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples
abstract	Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate
abstractGer	Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate
abstract_unstemmed	Takayuki Uchida,1,* Yoshio Kaku,2,* Hideyuki Hayasaka,1 Masaya Kofuji,1 Naoki Momose,1 Haruhisa Miyazawa,2 Yuichiro Ueda,2 Kiyonori Ito,2 Susumu Ookawara,2 Yoshiyuki Morishita2 1Department of Clinical Engineering, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan; 2Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, Saitama-City, Saitama, Japan*These authors contributed equally to this workCorrespondence: Yoshiyuki MorishitaDivision of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 1-847 Amanuma-cho, Omiya-ku, Saitama-City, Saitama 330-8503, JapanTel +81-48-647-2111Fax +81-48-647-6831Email ymorijichi.ac.jpBackground: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate.Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory.Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00+-0.01 EU/mL) with those obtained using the chromogenic substrate LAL test.Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.Keywords: endotoxin, dialysate, hemodialysis, limulus amebocyte lysate, chromogenic substrate
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title_short	Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples
url	https://doaj.org/article/b4d0fea4316740078975f9d4b3302dc5 https://www.dovepress.com/utility-of-an-automatic-limulus-amebocyte-lysate-kinetic-turbidimetric-peer-reviewed-article-MDER https://doaj.org/toc/1179-1470
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Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

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