Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions
<p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell s...
Ausführliche Beschreibung
Autor*in: |
Rivera-Molina Félix E [verfasserIn] Díaz-Blanco Nitza L [verfasserIn] Irizarry Rafael A [verfasserIn] Rodríguez-Quiñones José F [verfasserIn] Gómez-Garzón Diana [verfasserIn] Rodríguez-Medina José R [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2008 |
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Übergeordnetes Werk: |
In: BMC Genomics - BMC, 2003, 9(2008), 1, p 34 |
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Übergeordnetes Werk: |
volume:9 ; year:2008 ; number:1, p 34 |
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DOI / URN: |
10.1186/1471-2164-9-34 |
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Katalog-ID: |
DOAJ063158949 |
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520 | |a <p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< | ||
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10.1186/1471-2164-9-34 doi (DE-627)DOAJ063158949 (DE-599)DOAJ0ab0ce2ae1ae402bbd0424d90420b514 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Rivera-Molina Félix E verfasserin aut Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< Biotechnology Genetics Díaz-Blanco Nitza L verfasserin aut Irizarry Rafael A verfasserin aut Rodríguez-Quiñones José F verfasserin aut Gómez-Garzón Diana verfasserin aut Rodríguez-Medina José R verfasserin aut In BMC Genomics BMC, 2003 9(2008), 1, p 34 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:9 year:2008 number:1, p 34 https://doi.org/10.1186/1471-2164-9-34 kostenfrei https://doaj.org/article/0ab0ce2ae1ae402bbd0424d90420b514 kostenfrei http://www.biomedcentral.com/1471-2164/9/34 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1, p 34 |
spelling |
10.1186/1471-2164-9-34 doi (DE-627)DOAJ063158949 (DE-599)DOAJ0ab0ce2ae1ae402bbd0424d90420b514 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Rivera-Molina Félix E verfasserin aut Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< Biotechnology Genetics Díaz-Blanco Nitza L verfasserin aut Irizarry Rafael A verfasserin aut Rodríguez-Quiñones José F verfasserin aut Gómez-Garzón Diana verfasserin aut Rodríguez-Medina José R verfasserin aut In BMC Genomics BMC, 2003 9(2008), 1, p 34 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:9 year:2008 number:1, p 34 https://doi.org/10.1186/1471-2164-9-34 kostenfrei https://doaj.org/article/0ab0ce2ae1ae402bbd0424d90420b514 kostenfrei http://www.biomedcentral.com/1471-2164/9/34 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1, p 34 |
allfields_unstemmed |
10.1186/1471-2164-9-34 doi (DE-627)DOAJ063158949 (DE-599)DOAJ0ab0ce2ae1ae402bbd0424d90420b514 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Rivera-Molina Félix E verfasserin aut Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< Biotechnology Genetics Díaz-Blanco Nitza L verfasserin aut Irizarry Rafael A verfasserin aut Rodríguez-Quiñones José F verfasserin aut Gómez-Garzón Diana verfasserin aut Rodríguez-Medina José R verfasserin aut In BMC Genomics BMC, 2003 9(2008), 1, p 34 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:9 year:2008 number:1, p 34 https://doi.org/10.1186/1471-2164-9-34 kostenfrei https://doaj.org/article/0ab0ce2ae1ae402bbd0424d90420b514 kostenfrei http://www.biomedcentral.com/1471-2164/9/34 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1, p 34 |
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10.1186/1471-2164-9-34 doi (DE-627)DOAJ063158949 (DE-599)DOAJ0ab0ce2ae1ae402bbd0424d90420b514 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Rivera-Molina Félix E verfasserin aut Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< Biotechnology Genetics Díaz-Blanco Nitza L verfasserin aut Irizarry Rafael A verfasserin aut Rodríguez-Quiñones José F verfasserin aut Gómez-Garzón Diana verfasserin aut Rodríguez-Medina José R verfasserin aut In BMC Genomics BMC, 2003 9(2008), 1, p 34 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:9 year:2008 number:1, p 34 https://doi.org/10.1186/1471-2164-9-34 kostenfrei https://doaj.org/article/0ab0ce2ae1ae402bbd0424d90420b514 kostenfrei http://www.biomedcentral.com/1471-2164/9/34 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1, p 34 |
allfieldsSound |
10.1186/1471-2164-9-34 doi (DE-627)DOAJ063158949 (DE-599)DOAJ0ab0ce2ae1ae402bbd0424d90420b514 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Rivera-Molina Félix E verfasserin aut Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< Biotechnology Genetics Díaz-Blanco Nitza L verfasserin aut Irizarry Rafael A verfasserin aut Rodríguez-Quiñones José F verfasserin aut Gómez-Garzón Diana verfasserin aut Rodríguez-Medina José R verfasserin aut In BMC Genomics BMC, 2003 9(2008), 1, p 34 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:9 year:2008 number:1, p 34 https://doi.org/10.1186/1471-2164-9-34 kostenfrei https://doaj.org/article/0ab0ce2ae1ae402bbd0424d90420b514 kostenfrei http://www.biomedcentral.com/1471-2164/9/34 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2008 1, p 34 |
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Rivera-Molina Félix E @@aut@@ Díaz-Blanco Nitza L @@aut@@ Irizarry Rafael A @@aut@@ Rodríguez-Quiñones José F @@aut@@ Gómez-Garzón Diana @@aut@@ Rodríguez-Medina José R @@aut@@ |
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TP248.13-248.65 QH426-470 Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions |
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Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions |
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Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions |
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global mrna expression analysis in myosin ii deficient strains of <it<saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions |
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Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions |
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<p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< |
abstractGer |
<p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< |
abstract_unstemmed |
<p<Abstract</p< <p<Background</p< <p<The <it<Saccharomyces cerevisiae MYO1 </it<gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast. Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p< |
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Global mRNA expression analysis in myosin II deficient strains of <it<Saccharomyces cerevisiae </it<reveals an impairment of cell integrity functions |
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Myo1p deficiency in yeast (<it<myo1Δ</it<) causes a cell separation defect characterized by the formation of attached cells, yet it also causes abnormal budding patterns, formation of enlarged and elongated cells, increased osmotic sensitivity, delocalized chitin deposition, increased chitin synthesis, and hypersensitivity to the chitin synthase III inhibitor Nikkomycin Z. To determine how differential expression of genes is related to these diverse cell wall phenotypes, we analyzed the global mRNA expression profile of <it<myo1Δ </it<strains.</p< <p<Results</p< <p<Global mRNA expression profiles of <it<myo1Δ </it<strains and their corresponding wild type controls were obtained by hybridization to yeast oligonucleotide microarrays. Results for selected genes were confirmed by real time RT-PCR. A total of 547 differentially expressed genes (p ≤ 0.01) were identified with 263 up regulated and 284 down regulated genes in the <it<myo1Δ </it<strains. Gene set enrichment analysis revealed the significant over-representation of genes in the protein biosynthesis and stress response categories. The <it<SLT2/MPK1 </it<gene was up regulated in the microarray, and a <it<myo1Δslt2Δ </it<double mutant was non-viable. Overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31 </it<suppressed the hypersensitivity to Nikkomycin Z and increased the levels of phosphorylated Slt2p in <it<myo1Δ </it<strains. Increased levels of phosphorylated Slt2p were also observed in wild type strains under these conditions.</p< <p<Conclusion</p< <p<Following this analysis of global mRNA expression in yeast <it<myo1Δ </it<strains, we conclude that 547 genes were differentially regulated in <it<myo1Δ </it<strains and that the stress response and protein biosynthesis gene categories were coordinately regulated in this mutant. The <it<SLT2/MPK1 </it<gene was confirmed to be essential for <it<myo1Δ </it<strain viability, supporting that the up regulated stress response genes are regulated by the <it<PKC1 </it<cell integrity pathway. Suppression of Nikkomycin Z hypersensitivity together with Slt2p phosphorylation was caused by the overexpression of ribosomal protein genes <it<RPL30 </it<and <it<RPS31</it<. These ribosomal protein mRNAs were down regulated in the <it<myo1Δ </it<arrays, suggesting that down regulation of ribosomal biogenesis may affect cell integrity in <it<myo1Δ </it<strains.</p<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biotechnology</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Genetics</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Díaz-Blanco Nitza L</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Irizarry Rafael A</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Rodríguez-Quiñones José F</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Gómez-Garzón 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