Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice

<p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homoz...
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Autor*in:	Sancar Aziz [verfasserIn] Mankinen Richard W [verfasserIn] Lee Daekee [verfasserIn] Thompson Carol L [verfasserIn] Roberts Reade B [verfasserIn] Threadgill David W [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2006

Übergeordnetes Werk:	In: Journal of Circadian Rhythms - Ubiquity Press, 2015, 4(2006), 1, p 15
Übergeordnetes Werk:	volume:4 ; year:2006 ; number:1, p 15

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1740-3391-4-15

Katalog-ID:	DOAJ063290189

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allfields	10.1186/1740-3391-4-15 doi (DE-627)DOAJ063290189 (DE-599)DOAJ9487b3ddd73245cb98ced2c8c44afcd1 DE-627 ger DE-627 rakwb eng QH301-705.5 Sancar Aziz verfasserin aut Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p< Biology (General) Mankinen Richard W verfasserin aut Lee Daekee verfasserin aut Thompson Carol L verfasserin aut Roberts Reade B verfasserin aut Threadgill David W verfasserin aut In Journal of Circadian Rhythms Ubiquity Press, 2015 4(2006), 1, p 15 (DE-627)373752938 (DE-600)2126348-6 17403391 nnns volume:4 year:2006 number:1, p 15 https://doi.org/10.1186/1740-3391-4-15 kostenfrei https://doaj.org/article/9487b3ddd73245cb98ced2c8c44afcd1 kostenfrei http://www.jcircadianrhythms.com/content/4/1/15 kostenfrei https://doaj.org/toc/1740-3391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2006 1, p 15
spelling	10.1186/1740-3391-4-15 doi (DE-627)DOAJ063290189 (DE-599)DOAJ9487b3ddd73245cb98ced2c8c44afcd1 DE-627 ger DE-627 rakwb eng QH301-705.5 Sancar Aziz verfasserin aut Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p< Biology (General) Mankinen Richard W verfasserin aut Lee Daekee verfasserin aut Thompson Carol L verfasserin aut Roberts Reade B verfasserin aut Threadgill David W verfasserin aut In Journal of Circadian Rhythms Ubiquity Press, 2015 4(2006), 1, p 15 (DE-627)373752938 (DE-600)2126348-6 17403391 nnns volume:4 year:2006 number:1, p 15 https://doi.org/10.1186/1740-3391-4-15 kostenfrei https://doaj.org/article/9487b3ddd73245cb98ced2c8c44afcd1 kostenfrei http://www.jcircadianrhythms.com/content/4/1/15 kostenfrei https://doaj.org/toc/1740-3391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2006 1, p 15
allfields_unstemmed	10.1186/1740-3391-4-15 doi (DE-627)DOAJ063290189 (DE-599)DOAJ9487b3ddd73245cb98ced2c8c44afcd1 DE-627 ger DE-627 rakwb eng QH301-705.5 Sancar Aziz verfasserin aut Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p< Biology (General) Mankinen Richard W verfasserin aut Lee Daekee verfasserin aut Thompson Carol L verfasserin aut Roberts Reade B verfasserin aut Threadgill David W verfasserin aut In Journal of Circadian Rhythms Ubiquity Press, 2015 4(2006), 1, p 15 (DE-627)373752938 (DE-600)2126348-6 17403391 nnns volume:4 year:2006 number:1, p 15 https://doi.org/10.1186/1740-3391-4-15 kostenfrei https://doaj.org/article/9487b3ddd73245cb98ced2c8c44afcd1 kostenfrei http://www.jcircadianrhythms.com/content/4/1/15 kostenfrei https://doaj.org/toc/1740-3391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2006 1, p 15
allfieldsGer	10.1186/1740-3391-4-15 doi (DE-627)DOAJ063290189 (DE-599)DOAJ9487b3ddd73245cb98ced2c8c44afcd1 DE-627 ger DE-627 rakwb eng QH301-705.5 Sancar Aziz verfasserin aut Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p< Biology (General) Mankinen Richard W verfasserin aut Lee Daekee verfasserin aut Thompson Carol L verfasserin aut Roberts Reade B verfasserin aut Threadgill David W verfasserin aut In Journal of Circadian Rhythms Ubiquity Press, 2015 4(2006), 1, p 15 (DE-627)373752938 (DE-600)2126348-6 17403391 nnns volume:4 year:2006 number:1, p 15 https://doi.org/10.1186/1740-3391-4-15 kostenfrei https://doaj.org/article/9487b3ddd73245cb98ced2c8c44afcd1 kostenfrei http://www.jcircadianrhythms.com/content/4/1/15 kostenfrei https://doaj.org/toc/1740-3391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2006 1, p 15
allfieldsSound	10.1186/1740-3391-4-15 doi (DE-627)DOAJ063290189 (DE-599)DOAJ9487b3ddd73245cb98ced2c8c44afcd1 DE-627 ger DE-627 rakwb eng QH301-705.5 Sancar Aziz verfasserin aut Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p< Biology (General) Mankinen Richard W verfasserin aut Lee Daekee verfasserin aut Thompson Carol L verfasserin aut Roberts Reade B verfasserin aut Threadgill David W verfasserin aut In Journal of Circadian Rhythms Ubiquity Press, 2015 4(2006), 1, p 15 (DE-627)373752938 (DE-600)2126348-6 17403391 nnns volume:4 year:2006 number:1, p 15 https://doi.org/10.1186/1740-3391-4-15 kostenfrei https://doaj.org/article/9487b3ddd73245cb98ced2c8c44afcd1 kostenfrei http://www.jcircadianrhythms.com/content/4/1/15 kostenfrei https://doaj.org/toc/1740-3391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2006 1, p 15
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source	In Journal of Circadian Rhythms 4(2006), 1, p 15 volume:4 year:2006 number:1, p 15
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topic_title	QH301-705.5 Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice
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title	Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice
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title_full	Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice
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author_browse	Sancar Aziz Mankinen Richard W Lee Daekee Thompson Carol L Roberts Reade B Threadgill David W
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title_sort	wildtype epidermal growth factor receptor (<it<egfr</it<) is not required for daily locomotor or masking behavior in mice
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title_auth	Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice
abstract	<p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Recent studies have implicated the epidermal growth factor receptor (EGFR) within the subparaventricular zone as being a major mediator of locomotor and masking behaviors in mice. The results were based on small cohorts of mice homozygous for the hypomorphic <it<Egfr</it<<sup<<it<wa</it<2 </sup<allele on a mixed, genetically uncontrolled background, and on intraventricular infusion of exogenous EGFR ligands. Subsequenlty, a larger study using the same genetically mixed background failed to replicate the original findings. Since both previous approaches were susceptible to experimental artifacts related to an uncontrolled genetic background, we analyzed the locomotor behaviors in <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant mice on genetically defined, congenic backgrounds.</p< <p<Methods</p< <p<Mice carrying the <it<Egfr</it<<sup<<it<wa</it<2 </sup<hypomorphic allele were bred to congenicity by backcrossing greater than ten generations onto C57BL/6J and 129S1/SvImJ genetic backgrounds. Homozygous <it<Egfr</it<<sup<<it<wa</it<2 </sup<mutant and wildtype littermates were evaluated for defects in locomotor and masking behaviors.</p< <p<Results</p< <p<Mice homozygous for <it<Egfr</it<<sup<<it<wa</it<2 </sup<showed normal daily locomotor activity and masking indistinguishable from wildtype littermates at two light intensities (200–300 lux and 400–500 lux).</p< <p<Conclusion</p< <p<Our results demonstrate that reduced EGFR activity alone is insufficient to perturb locomotor and masking behaviors in mice. Our results also suggest that other uncontrolled genetic or environmental parameters confounded previous experiments linking EGFR activity to daily locomotor activity and provide a cautionary tale for genetically uncontrolled studies.</p<
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title_short	Wildtype epidermal growth factor receptor (<it<Egfr</it<) is not required for daily locomotor or masking behavior in mice
url	https://doi.org/10.1186/1740-3391-4-15 https://doaj.org/article/9487b3ddd73245cb98ced2c8c44afcd1 http://www.jcircadianrhythms.com/content/4/1/15 https://doaj.org/toc/1740-3391
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author2	Mankinen Richard W Lee Daekee Thompson Carol L Roberts Reade B Threadgill David W
author2Str	Mankinen Richard W Lee Daekee Thompson Carol L Roberts Reade B Threadgill David W
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doi_str	10.1186/1740-3391-4-15
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up_date	2024-07-03T16:52:54.047Z
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