Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model

Fulminant myocarditis causes impaired cardiac function, leading to poor prognosis and heart failure. Cell sheet engineering is an effective therapeutic option for improving cardiac function. Naïve blood mononuclear cells (MNCs) have been previously shown to enhance the quality and quantity of cellul...
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Gespeichert in:

Autor*in:	Kaori Sekine [verfasserIn] Akira T. Kawaguchi [verfasserIn] Masaki Miyazawa [verfasserIn] Haruo Hanawa [verfasserIn] Shinichi Matsuda [verfasserIn] Tetsuro Tamaki [verfasserIn] Takayuki Asahara [verfasserIn] Haruchika Masuda [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	anti-inflammation vasculogenic potential fulminant myocarditis cell sheet engineering

Übergeordnetes Werk:	In: Biology - MDPI AG, 2012, 11(2022), 1, p 106
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:1, p 106

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biology11010106

Katalog-ID:	DOAJ063387905

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520			\|a Fulminant myocarditis causes impaired cardiac function, leading to poor prognosis and heart failure. Cell sheet engineering is an effective therapeutic option for improving cardiac function. Naïve blood mononuclear cells (MNCs) have been previously shown to enhance the quality and quantity of cellular fractions (QQMNCs) with anti-inflammatory and vasculogenic potential using the one culture system. Herein, we investigated whether autologous cell sheet transplant with QQMNCs improves cardiac function in a rat model with experimental autoimmune myocarditis (EAM). Fibroblast sheets (F-sheet), prepared from EAM rats, were co-cultured with or without QQMNCs (QQ+F sheet) on temperature-responsive dishes. QQ+F sheet induced higher expression of anti-inflammatory and vasculogenic genes (<i<Vegf-b</i<, <i<Hgf</i<, <i<Il</i<<i<-10</i<, and <i<Mrc1/Cd206</i<) than the F sheet. EAM rats were transplanted with either QQ+F sheet or F-sheet, and the left ventricular (LV) hemodynamic analysis was performed using cardiac catheterization. Among the three groups (QQ+F sheet, F-sheet, operation control), the QQ+F sheet transplant group showed alleviation of end-diastolic pressure–volume relationship on a volume load to the same level as that in the healthy group. Histological analysis revealed that QQ+F sheet transplantation promoted revascularization and mitigated fibrosis by limiting LV remodeling. Therefore, autologous QQMNC-modified F-sheets may be a beneficial therapeutic option for EAM.
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spelling	10.3390/biology11010106 doi (DE-627)DOAJ063387905 (DE-599)DOAJ26041e16caf14e47860c42f34c96a5e4 DE-627 ger DE-627 rakwb eng QH301-705.5 Kaori Sekine verfasserin aut Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fulminant myocarditis causes impaired cardiac function, leading to poor prognosis and heart failure. Cell sheet engineering is an effective therapeutic option for improving cardiac function. Naïve blood mononuclear cells (MNCs) have been previously shown to enhance the quality and quantity of cellular fractions (QQMNCs) with anti-inflammatory and vasculogenic potential using the one culture system. Herein, we investigated whether autologous cell sheet transplant with QQMNCs improves cardiac function in a rat model with experimental autoimmune myocarditis (EAM). Fibroblast sheets (F-sheet), prepared from EAM rats, were co-cultured with or without QQMNCs (QQ+F sheet) on temperature-responsive dishes. QQ+F sheet induced higher expression of anti-inflammatory and vasculogenic genes (<i<Vegf-b</i<, <i<Hgf</i<, <i<Il</i<<i<-10</i<, and <i<Mrc1/Cd206</i<) than the F sheet. EAM rats were transplanted with either QQ+F sheet or F-sheet, and the left ventricular (LV) hemodynamic analysis was performed using cardiac catheterization. Among the three groups (QQ+F sheet, F-sheet, operation control), the QQ+F sheet transplant group showed alleviation of end-diastolic pressure–volume relationship on a volume load to the same level as that in the healthy group. Histological analysis revealed that QQ+F sheet transplantation promoted revascularization and mitigated fibrosis by limiting LV remodeling. Therefore, autologous QQMNC-modified F-sheets may be a beneficial therapeutic option for EAM. anti-inflammation vasculogenic potential fulminant myocarditis cell sheet engineering Biology (General) Akira T. Kawaguchi verfasserin aut Masaki Miyazawa verfasserin aut Haruo Hanawa verfasserin aut Shinichi Matsuda verfasserin aut Tetsuro Tamaki verfasserin aut Takayuki Asahara verfasserin aut Haruchika Masuda verfasserin aut In Biology MDPI AG, 2012 11(2022), 1, p 106 (DE-627)718622073 (DE-600)2661517-4 20797737 nnns volume:11 year:2022 number:1, p 106 https://doi.org/10.3390/biology11010106 kostenfrei https://doaj.org/article/26041e16caf14e47860c42f34c96a5e4 kostenfrei https://www.mdpi.com/2079-7737/11/1/106 kostenfrei https://doaj.org/toc/2079-7737 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 1, p 106
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authorswithroles_txt_mv	Kaori Sekine @@aut@@ Akira T. Kawaguchi @@aut@@ Masaki Miyazawa @@aut@@ Haruo Hanawa @@aut@@ Shinichi Matsuda @@aut@@ Tetsuro Tamaki @@aut@@ Takayuki Asahara @@aut@@ Haruchika Masuda @@aut@@
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callnumber-first	Q - Science
author	Kaori Sekine
spellingShingle	Kaori Sekine misc QH301-705.5 misc anti-inflammation misc vasculogenic potential misc fulminant myocarditis misc cell sheet engineering misc Biology (General) Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model
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topic_title	QH301-705.5 Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model anti-inflammation vasculogenic potential fulminant myocarditis cell sheet engineering
topic	misc QH301-705.5 misc anti-inflammation misc vasculogenic potential misc fulminant myocarditis misc cell sheet engineering misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc anti-inflammation misc vasculogenic potential misc fulminant myocarditis misc cell sheet engineering misc Biology (General)
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title	Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model
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title_full	Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model
author_sort	Kaori Sekine
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author_browse	Kaori Sekine Akira T. Kawaguchi Masaki Miyazawa Haruo Hanawa Shinichi Matsuda Tetsuro Tamaki Takayuki Asahara Haruchika Masuda
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title_sort	transplantation of fibroblast sheets with blood mononuclear cell culture exerts cardioprotective effects by enhancing anti-inflammation and vasculogenic potential in rat experimental autoimmune myocarditis model
callnumber	QH301-705.5
title_auth	Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model
abstract	Fulminant myocarditis causes impaired cardiac function, leading to poor prognosis and heart failure. Cell sheet engineering is an effective therapeutic option for improving cardiac function. Naïve blood mononuclear cells (MNCs) have been previously shown to enhance the quality and quantity of cellular fractions (QQMNCs) with anti-inflammatory and vasculogenic potential using the one culture system. Herein, we investigated whether autologous cell sheet transplant with QQMNCs improves cardiac function in a rat model with experimental autoimmune myocarditis (EAM). Fibroblast sheets (F-sheet), prepared from EAM rats, were co-cultured with or without QQMNCs (QQ+F sheet) on temperature-responsive dishes. QQ+F sheet induced higher expression of anti-inflammatory and vasculogenic genes (<i<Vegf-b</i<, <i<Hgf</i<, <i<Il</i<<i<-10</i<, and <i<Mrc1/Cd206</i<) than the F sheet. EAM rats were transplanted with either QQ+F sheet or F-sheet, and the left ventricular (LV) hemodynamic analysis was performed using cardiac catheterization. Among the three groups (QQ+F sheet, F-sheet, operation control), the QQ+F sheet transplant group showed alleviation of end-diastolic pressure–volume relationship on a volume load to the same level as that in the healthy group. Histological analysis revealed that QQ+F sheet transplantation promoted revascularization and mitigated fibrosis by limiting LV remodeling. Therefore, autologous QQMNC-modified F-sheets may be a beneficial therapeutic option for EAM.
abstractGer	Fulminant myocarditis causes impaired cardiac function, leading to poor prognosis and heart failure. Cell sheet engineering is an effective therapeutic option for improving cardiac function. Naïve blood mononuclear cells (MNCs) have been previously shown to enhance the quality and quantity of cellular fractions (QQMNCs) with anti-inflammatory and vasculogenic potential using the one culture system. Herein, we investigated whether autologous cell sheet transplant with QQMNCs improves cardiac function in a rat model with experimental autoimmune myocarditis (EAM). Fibroblast sheets (F-sheet), prepared from EAM rats, were co-cultured with or without QQMNCs (QQ+F sheet) on temperature-responsive dishes. QQ+F sheet induced higher expression of anti-inflammatory and vasculogenic genes (<i<Vegf-b</i<, <i<Hgf</i<, <i<Il</i<<i<-10</i<, and <i<Mrc1/Cd206</i<) than the F sheet. EAM rats were transplanted with either QQ+F sheet or F-sheet, and the left ventricular (LV) hemodynamic analysis was performed using cardiac catheterization. Among the three groups (QQ+F sheet, F-sheet, operation control), the QQ+F sheet transplant group showed alleviation of end-diastolic pressure–volume relationship on a volume load to the same level as that in the healthy group. Histological analysis revealed that QQ+F sheet transplantation promoted revascularization and mitigated fibrosis by limiting LV remodeling. Therefore, autologous QQMNC-modified F-sheets may be a beneficial therapeutic option for EAM.
abstract_unstemmed	Fulminant myocarditis causes impaired cardiac function, leading to poor prognosis and heart failure. Cell sheet engineering is an effective therapeutic option for improving cardiac function. Naïve blood mononuclear cells (MNCs) have been previously shown to enhance the quality and quantity of cellular fractions (QQMNCs) with anti-inflammatory and vasculogenic potential using the one culture system. Herein, we investigated whether autologous cell sheet transplant with QQMNCs improves cardiac function in a rat model with experimental autoimmune myocarditis (EAM). Fibroblast sheets (F-sheet), prepared from EAM rats, were co-cultured with or without QQMNCs (QQ+F sheet) on temperature-responsive dishes. QQ+F sheet induced higher expression of anti-inflammatory and vasculogenic genes (<i<Vegf-b</i<, <i<Hgf</i<, <i<Il</i<<i<-10</i<, and <i<Mrc1/Cd206</i<) than the F sheet. EAM rats were transplanted with either QQ+F sheet or F-sheet, and the left ventricular (LV) hemodynamic analysis was performed using cardiac catheterization. Among the three groups (QQ+F sheet, F-sheet, operation control), the QQ+F sheet transplant group showed alleviation of end-diastolic pressure–volume relationship on a volume load to the same level as that in the healthy group. Histological analysis revealed that QQ+F sheet transplantation promoted revascularization and mitigated fibrosis by limiting LV remodeling. Therefore, autologous QQMNC-modified F-sheets may be a beneficial therapeutic option for EAM.
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title_short	Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model
url	https://doi.org/10.3390/biology11010106 https://doaj.org/article/26041e16caf14e47860c42f34c96a5e4 https://www.mdpi.com/2079-7737/11/1/106 https://doaj.org/toc/2079-7737
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author2	Akira T. Kawaguchi Masaki Miyazawa Haruo Hanawa Shinichi Matsuda Tetsuro Tamaki Takayuki Asahara Haruchika Masuda
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up_date	2024-07-03T17:26:27.074Z
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Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model

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