PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA

Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV)...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Alaa H. Al-Charrakh [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Rotavirus Patients Diarrhea VP6 protein NSP4 RT-PCR.

Übergeordnetes Werk:	In: Euromediterranean Biomedical Journal - Associazione Italiana Giovani Medici, 2013, 14(2019), 15, Seite 65-69
Übergeordnetes Werk:	volume:14 ; year:2019 ; number:15 ; pages:65-69

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3269/1970-5492.2019.14.15

Katalog-ID:	DOAJ063445646

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520			\|a Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children.
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allfields	10.3269/1970-5492.2019.14.15 doi (DE-627)DOAJ063445646 (DE-599)DOAJ5a2cbf5694e94c10b8890cee4c45c79a DE-627 ger DE-627 rakwb eng R5-920 Alaa H. Al-Charrakh verfasserin aut PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children. Rotavirus Patients Diarrhea VP6 protein NSP4 RT-PCR. Medicine (General) In Euromediterranean Biomedical Journal Associazione Italiana Giovani Medici, 2013 14(2019), 15, Seite 65-69 (DE-627)756823900 (DE-600)2727252-7 22797165 nnns volume:14 year:2019 number:15 pages:65-69 https://doi.org/10.3269/1970-5492.2019.14.15 kostenfrei https://doaj.org/article/5a2cbf5694e94c10b8890cee4c45c79a kostenfrei http://www.embj.org/embj/prevalence-and-molecular-characterization-of-rotavirus-a-in-pediatric-patients-with-acute-diarrhea/ kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2019 15 65-69
spelling	10.3269/1970-5492.2019.14.15 doi (DE-627)DOAJ063445646 (DE-599)DOAJ5a2cbf5694e94c10b8890cee4c45c79a DE-627 ger DE-627 rakwb eng R5-920 Alaa H. Al-Charrakh verfasserin aut PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children. Rotavirus Patients Diarrhea VP6 protein NSP4 RT-PCR. Medicine (General) In Euromediterranean Biomedical Journal Associazione Italiana Giovani Medici, 2013 14(2019), 15, Seite 65-69 (DE-627)756823900 (DE-600)2727252-7 22797165 nnns volume:14 year:2019 number:15 pages:65-69 https://doi.org/10.3269/1970-5492.2019.14.15 kostenfrei https://doaj.org/article/5a2cbf5694e94c10b8890cee4c45c79a kostenfrei http://www.embj.org/embj/prevalence-and-molecular-characterization-of-rotavirus-a-in-pediatric-patients-with-acute-diarrhea/ kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2019 15 65-69
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allfieldsGer	10.3269/1970-5492.2019.14.15 doi (DE-627)DOAJ063445646 (DE-599)DOAJ5a2cbf5694e94c10b8890cee4c45c79a DE-627 ger DE-627 rakwb eng R5-920 Alaa H. Al-Charrakh verfasserin aut PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children. Rotavirus Patients Diarrhea VP6 protein NSP4 RT-PCR. Medicine (General) In Euromediterranean Biomedical Journal Associazione Italiana Giovani Medici, 2013 14(2019), 15, Seite 65-69 (DE-627)756823900 (DE-600)2727252-7 22797165 nnns volume:14 year:2019 number:15 pages:65-69 https://doi.org/10.3269/1970-5492.2019.14.15 kostenfrei https://doaj.org/article/5a2cbf5694e94c10b8890cee4c45c79a kostenfrei http://www.embj.org/embj/prevalence-and-molecular-characterization-of-rotavirus-a-in-pediatric-patients-with-acute-diarrhea/ kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2019 15 65-69
allfieldsSound	10.3269/1970-5492.2019.14.15 doi (DE-627)DOAJ063445646 (DE-599)DOAJ5a2cbf5694e94c10b8890cee4c45c79a DE-627 ger DE-627 rakwb eng R5-920 Alaa H. Al-Charrakh verfasserin aut PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children. Rotavirus Patients Diarrhea VP6 protein NSP4 RT-PCR. Medicine (General) In Euromediterranean Biomedical Journal Associazione Italiana Giovani Medici, 2013 14(2019), 15, Seite 65-69 (DE-627)756823900 (DE-600)2727252-7 22797165 nnns volume:14 year:2019 number:15 pages:65-69 https://doi.org/10.3269/1970-5492.2019.14.15 kostenfrei https://doaj.org/article/5a2cbf5694e94c10b8890cee4c45c79a kostenfrei http://www.embj.org/embj/prevalence-and-molecular-characterization-of-rotavirus-a-in-pediatric-patients-with-acute-diarrhea/ kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei https://doaj.org/toc/2279-7165 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 14 2019 15 65-69
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callnumber-first	R - Medicine
author	Alaa H. Al-Charrakh
spellingShingle	Alaa H. Al-Charrakh misc R5-920 misc Rotavirus misc Patients misc Diarrhea misc VP6 protein misc NSP4 misc RT-PCR. misc Medicine (General) PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA
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topic_title	R5-920 PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA Rotavirus Patients Diarrhea VP6 protein NSP4 RT-PCR
topic	misc R5-920 misc Rotavirus misc Patients misc Diarrhea misc VP6 protein misc NSP4 misc RT-PCR. misc Medicine (General)
topic_unstemmed	misc R5-920 misc Rotavirus misc Patients misc Diarrhea misc VP6 protein misc NSP4 misc RT-PCR. misc Medicine (General)
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title	PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA
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title_full	PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA
author_sort	Alaa H. Al-Charrakh
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title_sort	prevalence and molecular characterization of rotavirus a in pediatric patients with acute diarrhea
callnumber	R5-920
title_auth	PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA
abstract	Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children.
abstractGer	Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children.
abstract_unstemmed	Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4 gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in stool specimens. The results revealed that males tend to be more effected by RV than females and the bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The infection rate had increased with decreasing the age of the children.
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title_short	PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA
url	https://doi.org/10.3269/1970-5492.2019.14.15 https://doaj.org/article/5a2cbf5694e94c10b8890cee4c45c79a http://www.embj.org/embj/prevalence-and-molecular-characterization-of-rotavirus-a-in-pediatric-patients-with-acute-diarrhea/ https://doaj.org/toc/2279-7165
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PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA

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