Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase
ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interaction...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
TELISSA C. KASSAR [verfasserIn] TEREZA MAGALHÃES [verfasserIn] JOSÉ V.J. S. JÚNIOR [verfasserIn] AMANDA G.O. CARVALHO [verfasserIn] ANDRÉA N.M.R. DA SILVA [verfasserIn] SABRINA R.A. QUEIROZ [verfasserIn] GIOVANI R. BERTANI [verfasserIn] LAURA H.V.G. GIL [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
In: Anais da Academia Brasileira de Ciências - Academia Brasileira de Ciências, 2004 |
|---|
| Links: |
|---|
| DOI / URN: |
10.1590/0001-3765201720160196 |
|---|
| Katalog-ID: |
DOAJ063778491 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | DOAJ063778491 | ||
| 003 | DE-627 | ||
| 005 | 20230502074210.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230228nuuuuuuuuxx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1590/0001-3765201720160196 |2 doi | |
| 035 | |a (DE-627)DOAJ063778491 | ||
| 035 | |a (DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 0 | |a TELISSA C. KASSAR |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. | ||
| 650 | 4 | |a Gaussia luciferase | |
| 650 | 4 | |a homologous recombination in yeast | |
| 650 | 4 | |a reporter gene | |
| 650 | 4 | |a yellow fever virus | |
| 653 | 0 | |a Science | |
| 653 | 0 | |a Q | |
| 700 | 0 | |a TEREZA MAGALHÃES |e verfasserin |4 aut | |
| 700 | 0 | |a JOSÉ V.J. S. JÚNIOR |e verfasserin |4 aut | |
| 700 | 0 | |a AMANDA G.O. CARVALHO |e verfasserin |4 aut | |
| 700 | 0 | |a ANDRÉA N.M.R. DA SILVA |e verfasserin |4 aut | |
| 700 | 0 | |a SABRINA R.A. QUEIROZ |e verfasserin |4 aut | |
| 700 | 0 | |a GIOVANI R. BERTANI |e verfasserin |4 aut | |
| 700 | 0 | |a LAURA H.V.G. GIL |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t Anais da Academia Brasileira de Ciências |d Academia Brasileira de Ciências, 2004 |w (DE-627)329269984 |w (DE-600)2046885-4 |x 16782690 |7 nnns |
| 856 | 4 | 0 | |u https://doi.org/10.1590/0001-3765201720160196 |z kostenfrei |
| 856 | 4 | 0 | |u https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323 |z kostenfrei |
| 856 | 4 | 0 | |u http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/1678-2690 |y Journal toc |z kostenfrei |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_DOAJ | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| author_variant |
t c k tck t m tm j v s j jvsj a g c agc a n d s ands s r q srq g r b grb l h g lhg |
|---|---|
| matchkey_str |
article:16782690:uuuuuuuu::osrcinncaatrztooaeobnnylofvriusale |
| allfields |
10.1590/0001-3765201720160196 doi (DE-627)DOAJ063778491 (DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323 DE-627 ger DE-627 rakwb eng TELISSA C. KASSAR verfasserin aut Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus Science Q TEREZA MAGALHÃES verfasserin aut JOSÉ V.J. S. JÚNIOR verfasserin aut AMANDA G.O. CARVALHO verfasserin aut ANDRÉA N.M.R. DA SILVA verfasserin aut SABRINA R.A. QUEIROZ verfasserin aut GIOVANI R. BERTANI verfasserin aut LAURA H.V.G. GIL verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns https://doi.org/10.1590/0001-3765201720160196 kostenfrei https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
| spelling |
10.1590/0001-3765201720160196 doi (DE-627)DOAJ063778491 (DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323 DE-627 ger DE-627 rakwb eng TELISSA C. KASSAR verfasserin aut Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus Science Q TEREZA MAGALHÃES verfasserin aut JOSÉ V.J. S. JÚNIOR verfasserin aut AMANDA G.O. CARVALHO verfasserin aut ANDRÉA N.M.R. DA SILVA verfasserin aut SABRINA R.A. QUEIROZ verfasserin aut GIOVANI R. BERTANI verfasserin aut LAURA H.V.G. GIL verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns https://doi.org/10.1590/0001-3765201720160196 kostenfrei https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
| allfields_unstemmed |
10.1590/0001-3765201720160196 doi (DE-627)DOAJ063778491 (DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323 DE-627 ger DE-627 rakwb eng TELISSA C. KASSAR verfasserin aut Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus Science Q TEREZA MAGALHÃES verfasserin aut JOSÉ V.J. S. JÚNIOR verfasserin aut AMANDA G.O. CARVALHO verfasserin aut ANDRÉA N.M.R. DA SILVA verfasserin aut SABRINA R.A. QUEIROZ verfasserin aut GIOVANI R. BERTANI verfasserin aut LAURA H.V.G. GIL verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns https://doi.org/10.1590/0001-3765201720160196 kostenfrei https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
| allfieldsGer |
10.1590/0001-3765201720160196 doi (DE-627)DOAJ063778491 (DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323 DE-627 ger DE-627 rakwb eng TELISSA C. KASSAR verfasserin aut Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus Science Q TEREZA MAGALHÃES verfasserin aut JOSÉ V.J. S. JÚNIOR verfasserin aut AMANDA G.O. CARVALHO verfasserin aut ANDRÉA N.M.R. DA SILVA verfasserin aut SABRINA R.A. QUEIROZ verfasserin aut GIOVANI R. BERTANI verfasserin aut LAURA H.V.G. GIL verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns https://doi.org/10.1590/0001-3765201720160196 kostenfrei https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
| allfieldsSound |
10.1590/0001-3765201720160196 doi (DE-627)DOAJ063778491 (DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323 DE-627 ger DE-627 rakwb eng TELISSA C. KASSAR verfasserin aut Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus Science Q TEREZA MAGALHÃES verfasserin aut JOSÉ V.J. S. JÚNIOR verfasserin aut AMANDA G.O. CARVALHO verfasserin aut ANDRÉA N.M.R. DA SILVA verfasserin aut SABRINA R.A. QUEIROZ verfasserin aut GIOVANI R. BERTANI verfasserin aut LAURA H.V.G. GIL verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns https://doi.org/10.1590/0001-3765201720160196 kostenfrei https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
| language |
English |
| source |
In Anais da Academia Brasileira de Ciências |
| sourceStr |
In Anais da Academia Brasileira de Ciências |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus Science Q |
| isfreeaccess_bool |
true |
| container_title |
Anais da Academia Brasileira de Ciências |
| authorswithroles_txt_mv |
TELISSA C. KASSAR @@aut@@ TEREZA MAGALHÃES @@aut@@ JOSÉ V.J. S. JÚNIOR @@aut@@ AMANDA G.O. CARVALHO @@aut@@ ANDRÉA N.M.R. DA SILVA @@aut@@ SABRINA R.A. QUEIROZ @@aut@@ GIOVANI R. BERTANI @@aut@@ LAURA H.V.G. GIL @@aut@@ |
| publishDateDaySort_date |
2024-01-01T00:00:00Z |
| hierarchy_top_id |
329269984 |
| id |
DOAJ063778491 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ063778491</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230502074210.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228nuuuuuuuuxx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1590/0001-3765201720160196</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ063778491</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">TELISSA C. KASSAR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gaussia luciferase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">homologous recombination in yeast</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">reporter gene</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">yellow fever virus</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Science</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Q</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">TEREZA MAGALHÃES</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">JOSÉ V.J. S. JÚNIOR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">AMANDA G.O. CARVALHO</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">ANDRÉA N.M.R. DA SILVA</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">SABRINA R.A. QUEIROZ</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">GIOVANI R. BERTANI</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">LAURA H.V.G. GIL</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Anais da Academia Brasileira de Ciências</subfield><subfield code="d">Academia Brasileira de Ciências, 2004</subfield><subfield code="w">(DE-627)329269984</subfield><subfield code="w">(DE-600)2046885-4</subfield><subfield code="x">16782690</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1590/0001-3765201720160196</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1678-2690</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield></record></collection>
|
| author |
TELISSA C. KASSAR |
| spellingShingle |
TELISSA C. KASSAR misc Gaussia luciferase misc homologous recombination in yeast misc reporter gene misc yellow fever virus misc Science misc Q Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| authorStr |
TELISSA C. KASSAR |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)329269984 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut aut aut aut aut |
| collection |
DOAJ |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
16782690 |
| topic_title |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Gaussia luciferase homologous recombination in yeast reporter gene yellow fever virus |
| topic |
misc Gaussia luciferase misc homologous recombination in yeast misc reporter gene misc yellow fever virus misc Science misc Q |
| topic_unstemmed |
misc Gaussia luciferase misc homologous recombination in yeast misc reporter gene misc yellow fever virus misc Science misc Q |
| topic_browse |
misc Gaussia luciferase misc homologous recombination in yeast misc reporter gene misc yellow fever virus misc Science misc Q |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
Anais da Academia Brasileira de Ciências |
| hierarchy_parent_id |
329269984 |
| hierarchy_top_title |
Anais da Academia Brasileira de Ciências |
| isfreeaccess_txt |
true |
| familylinks_str_mv |
(DE-627)329269984 (DE-600)2046885-4 |
| title |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| ctrlnum |
(DE-627)DOAJ063778491 (DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323 |
| title_full |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| author_sort |
TELISSA C. KASSAR |
| journal |
Anais da Academia Brasileira de Ciências |
| journalStr |
Anais da Academia Brasileira de Ciências |
| lang_code |
eng |
| isOA_bool |
true |
| recordtype |
marc |
| contenttype_str_mv |
txt |
| author_browse |
TELISSA C. KASSAR TEREZA MAGALHÃES JOSÉ V.J. S. JÚNIOR AMANDA G.O. CARVALHO ANDRÉA N.M.R. DA SILVA SABRINA R.A. QUEIROZ GIOVANI R. BERTANI LAURA H.V.G. GIL |
| format_se |
Elektronische Aufsätze |
| author-letter |
TELISSA C. KASSAR |
| doi_str_mv |
10.1590/0001-3765201720160196 |
| author2-role |
verfasserin |
| title_sort |
construction and characterization of a recombinant yellow fever virus stably expressing gaussia luciferase |
| title_auth |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| abstract |
ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. |
| abstractGer |
ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. |
| abstract_unstemmed |
ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA |
| title_short |
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase |
| url |
https://doi.org/10.1590/0001-3765201720160196 https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en https://doaj.org/toc/1678-2690 |
| remote_bool |
true |
| author2 |
TEREZA MAGALHÃES JOSÉ V.J. S. JÚNIOR AMANDA G.O. CARVALHO ANDRÉA N.M.R. DA SILVA SABRINA R.A. QUEIROZ GIOVANI R. BERTANI LAURA H.V.G. GIL |
| author2Str |
TEREZA MAGALHÃES JOSÉ V.J. S. JÚNIOR AMANDA G.O. CARVALHO ANDRÉA N.M.R. DA SILVA SABRINA R.A. QUEIROZ GIOVANI R. BERTANI LAURA H.V.G. GIL |
| ppnlink |
329269984 |
| mediatype_str_mv |
c |
| isOA_txt |
true |
| hochschulschrift_bool |
false |
| doi_str |
10.1590/0001-3765201720160196 |
| up_date |
2024-07-03T19:30:35.986Z |
| _version_ |
1803587457895104512 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ063778491</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230502074210.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228nuuuuuuuuxx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1590/0001-3765201720160196</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ063778491</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ90dd2e57000d45e6b24b4cf512586323</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">TELISSA C. KASSAR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc expression and viral replication was observed (r2=0.9967), indicating that measurement of GLuc activity may be used to assess viral replication in different applications. In addition, we evaluated the use of the recombinant virus in an antiviral assay with recombinant human alfa-2b interferon. A 60% inhibition of GLuc expression was observed in cells infected with YFV-GLuc and incubated with IFN alfa-2b. Previously tested on YFV inhibition by plaque assays indicated a similar fold-decrease in viral replication. These results are valuable as they show the stability of YFV-GLuc and one of several possible applications of this construct.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gaussia luciferase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">homologous recombination in yeast</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">reporter gene</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">yellow fever virus</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Science</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Q</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">TEREZA MAGALHÃES</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">JOSÉ V.J. S. JÚNIOR</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">AMANDA G.O. CARVALHO</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">ANDRÉA N.M.R. DA SILVA</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">SABRINA R.A. QUEIROZ</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">GIOVANI R. BERTANI</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">LAURA H.V.G. GIL</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Anais da Academia Brasileira de Ciências</subfield><subfield code="d">Academia Brasileira de Ciências, 2004</subfield><subfield code="w">(DE-627)329269984</subfield><subfield code="w">(DE-600)2046885-4</subfield><subfield code="x">16782690</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1590/0001-3765201720160196</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/90dd2e57000d45e6b24b4cf512586323</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012101&lng=en&tlng=en</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1678-2690</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield></record></collection>
|
| score |
7.401758 |