The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1

<p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Ukena Sya N [verfasserIn] Westendorf Astrid M [verfasserIn] Hansen Wiebke [verfasserIn] Rohde Manfred [verfasserIn] Geffers Robert [verfasserIn] Coldewey Sina [verfasserIn] Suerbaum Sebastian [verfasserIn] Buer Jan [verfasserIn] Gunzer Florian [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2005

Übergeordnetes Werk:	In: BMC Medical Genetics - BMC, 2003, 6(2005), 1, p 43
Übergeordnetes Werk:	volume:6 ; year:2005 ; number:1, p 43

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2350-6-43

Katalog-ID:	DOAJ063864274

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author_variant	u s n usn w a m wam h w hw r m rm g r gr c s cs s s ss b j bj g f gf
matchkey_str	article:14712350:2005----::hhsrsostterboiieceihaoittanise97pcfcpeuainf
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publishDate	2005
allfields	10.1186/1471-2350-6-43 doi (DE-627)DOAJ063864274 (DE-599)DOAJ8a0f580d8db34c039e67c43ac5b6f379 DE-627 ger DE-627 rakwb eng RC31-1245 QH426-470 Ukena Sya N verfasserin aut The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p< Internal medicine Genetics Westendorf Astrid M verfasserin aut Hansen Wiebke verfasserin aut Rohde Manfred verfasserin aut Geffers Robert verfasserin aut Coldewey Sina verfasserin aut Suerbaum Sebastian verfasserin aut Buer Jan verfasserin aut Gunzer Florian verfasserin aut In BMC Medical Genetics BMC, 2003 6(2005), 1, p 43 (DE-627)326643788 (DE-600)2041359-2 14712350 nnns volume:6 year:2005 number:1, p 43 https://doi.org/10.1186/1471-2350-6-43 kostenfrei https://doaj.org/article/8a0f580d8db34c039e67c43ac5b6f379 kostenfrei http://www.biomedcentral.com/1471-2350/6/43 kostenfrei https://doaj.org/toc/1471-2350 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 43
spelling	10.1186/1471-2350-6-43 doi (DE-627)DOAJ063864274 (DE-599)DOAJ8a0f580d8db34c039e67c43ac5b6f379 DE-627 ger DE-627 rakwb eng RC31-1245 QH426-470 Ukena Sya N verfasserin aut The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p< Internal medicine Genetics Westendorf Astrid M verfasserin aut Hansen Wiebke verfasserin aut Rohde Manfred verfasserin aut Geffers Robert verfasserin aut Coldewey Sina verfasserin aut Suerbaum Sebastian verfasserin aut Buer Jan verfasserin aut Gunzer Florian verfasserin aut In BMC Medical Genetics BMC, 2003 6(2005), 1, p 43 (DE-627)326643788 (DE-600)2041359-2 14712350 nnns volume:6 year:2005 number:1, p 43 https://doi.org/10.1186/1471-2350-6-43 kostenfrei https://doaj.org/article/8a0f580d8db34c039e67c43ac5b6f379 kostenfrei http://www.biomedcentral.com/1471-2350/6/43 kostenfrei https://doaj.org/toc/1471-2350 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 43
allfields_unstemmed	10.1186/1471-2350-6-43 doi (DE-627)DOAJ063864274 (DE-599)DOAJ8a0f580d8db34c039e67c43ac5b6f379 DE-627 ger DE-627 rakwb eng RC31-1245 QH426-470 Ukena Sya N verfasserin aut The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p< Internal medicine Genetics Westendorf Astrid M verfasserin aut Hansen Wiebke verfasserin aut Rohde Manfred verfasserin aut Geffers Robert verfasserin aut Coldewey Sina verfasserin aut Suerbaum Sebastian verfasserin aut Buer Jan verfasserin aut Gunzer Florian verfasserin aut In BMC Medical Genetics BMC, 2003 6(2005), 1, p 43 (DE-627)326643788 (DE-600)2041359-2 14712350 nnns volume:6 year:2005 number:1, p 43 https://doi.org/10.1186/1471-2350-6-43 kostenfrei https://doaj.org/article/8a0f580d8db34c039e67c43ac5b6f379 kostenfrei http://www.biomedcentral.com/1471-2350/6/43 kostenfrei https://doaj.org/toc/1471-2350 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 43
allfieldsGer	10.1186/1471-2350-6-43 doi (DE-627)DOAJ063864274 (DE-599)DOAJ8a0f580d8db34c039e67c43ac5b6f379 DE-627 ger DE-627 rakwb eng RC31-1245 QH426-470 Ukena Sya N verfasserin aut The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p< Internal medicine Genetics Westendorf Astrid M verfasserin aut Hansen Wiebke verfasserin aut Rohde Manfred verfasserin aut Geffers Robert verfasserin aut Coldewey Sina verfasserin aut Suerbaum Sebastian verfasserin aut Buer Jan verfasserin aut Gunzer Florian verfasserin aut In BMC Medical Genetics BMC, 2003 6(2005), 1, p 43 (DE-627)326643788 (DE-600)2041359-2 14712350 nnns volume:6 year:2005 number:1, p 43 https://doi.org/10.1186/1471-2350-6-43 kostenfrei https://doaj.org/article/8a0f580d8db34c039e67c43ac5b6f379 kostenfrei http://www.biomedcentral.com/1471-2350/6/43 kostenfrei https://doaj.org/toc/1471-2350 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 43
allfieldsSound	10.1186/1471-2350-6-43 doi (DE-627)DOAJ063864274 (DE-599)DOAJ8a0f580d8db34c039e67c43ac5b6f379 DE-627 ger DE-627 rakwb eng RC31-1245 QH426-470 Ukena Sya N verfasserin aut The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p< Internal medicine Genetics Westendorf Astrid M verfasserin aut Hansen Wiebke verfasserin aut Rohde Manfred verfasserin aut Geffers Robert verfasserin aut Coldewey Sina verfasserin aut Suerbaum Sebastian verfasserin aut Buer Jan verfasserin aut Gunzer Florian verfasserin aut In BMC Medical Genetics BMC, 2003 6(2005), 1, p 43 (DE-627)326643788 (DE-600)2041359-2 14712350 nnns volume:6 year:2005 number:1, p 43 https://doi.org/10.1186/1471-2350-6-43 kostenfrei https://doaj.org/article/8a0f580d8db34c039e67c43ac5b6f379 kostenfrei http://www.biomedcentral.com/1471-2350/6/43 kostenfrei https://doaj.org/toc/1471-2350 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2005 1, p 43
language	English
source	In BMC Medical Genetics 6(2005), 1, p 43 volume:6 year:2005 number:1, p 43
sourceStr	In BMC Medical Genetics 6(2005), 1, p 43 volume:6 year:2005 number:1, p 43
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Internal medicine Genetics
isfreeaccess_bool	true
container_title	BMC Medical Genetics
authorswithroles_txt_mv	Ukena Sya N @@aut@@ Westendorf Astrid M @@aut@@ Hansen Wiebke @@aut@@ Rohde Manfred @@aut@@ Geffers Robert @@aut@@ Coldewey Sina @@aut@@ Suerbaum Sebastian @@aut@@ Buer Jan @@aut@@ Gunzer Florian @@aut@@
publishDateDaySort_date	2005-01-01T00:00:00Z
hierarchy_top_id	326643788
id	DOAJ063864274
language_de	englisch
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The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. 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author	Ukena Sya N
spellingShingle	Ukena Sya N misc RC31-1245 misc QH426-470 misc Internal medicine misc Genetics The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1
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topic_title	RC31-1245 QH426-470 The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1
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title	The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1
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title_full	The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1
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author_browse	Ukena Sya N Westendorf Astrid M Hansen Wiebke Rohde Manfred Geffers Robert Coldewey Sina Suerbaum Sebastian Buer Jan Gunzer Florian
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title_sort	host response to the probiotic <it<escherichia coli </it<strain nissle 1917: specific up-regulation of the proinflammatory chemokine mcp-1
callnumber	RC31-1245
title_auth	The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1
abstract	<p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<The use of live microorganisms to influence positively the course of intestinal disorders such as infectious diarrhea or chronic inflammatory conditions has recently gained increasing interest as a therapeutic alternative. <it<In vitro </it<and <it<in vivo </it<investigations have demonstrated that probiotic-host eukaryotic cell interactions evoke a large number of responses potentially responsible for the effects of probiotics. The aim of this study was to improve our understanding of the <it<E. coli </it<Nissle 1917-host interaction by analyzing the gene expression pattern initiated by this probiotic in human intestinal epithelial cells.</p< <p<Methods</p< <p<Gene expression profiles of Caco-2 cells treated with <it<E. coli </it<Nissle 1917 were analyzed with microarrays. A second human intestinal cell line and also pieces of small intestine from BALB/c mice were used to confirm regulatory data of selected genes by real-time RT-PCR and cytometric bead array (CBA) to detect secretion of corresponding proteins.</p< <p<Results</p< <p<Whole genome expression analysis revealed 126 genes specifically regulated after treatment of confluent Caco-2 cells with <it<E. coli </it<Nissle 1917. Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. MCP-1 gene expression was also up-regulated in mouse intestinal tissue.</p< <p<Conclusion</p< <p<Thus, probiotic <it<E. coli </it<Nissle 1917 specifically upregulates expression of proinflammatory genes and proteins in human and mouse intestinal epithelial cells.</p<
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title_short	The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1
url	https://doi.org/10.1186/1471-2350-6-43 https://doaj.org/article/8a0f580d8db34c039e67c43ac5b6f379 http://www.biomedcentral.com/1471-2350/6/43 https://doaj.org/toc/1471-2350
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Among others, expression of genes encoding the proinflammatory molecules monocyte chemoattractant protein-1 ligand 2 (MCP-1), macrophage inflammatory protein-2 alpha (MIP-2α) and macrophage inflammatory protein-2 beta (MIP-2β) was increased up to 10 fold. Caco-2 cells cocultured with <it<E. coli </it<Nissle 1917 also secreted high amounts of MCP-1 protein. Elevated levels of MCP-1 and MIP-2α mRNA could be confirmed with Lovo cells. 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The host response to the probiotic <it<Escherichia coli </it<strain Nissle 1917: Specific up-regulation of the proinflammatory chemokine MCP-1

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