Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli

Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Shima Hassan Baien [verfasserIn] Jana Seele [verfasserIn] Timo Henneck [verfasserIn] Christin Freibrodt [verfasserIn] György Szura [verfasserIn] Hani Moubasher [verfasserIn] Roland Nau [verfasserIn] Graham Brogden [verfasserIn] Matthias Mörgelin [verfasserIn] Mahavir Singh [verfasserIn] Manfred Kietzmann [verfasserIn] Maren von Köckritz-Blickwede [verfasserIn] Nicole de Buhr [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 10(2020)
Übergeordnetes Werk:	volume:10 ; year:2020

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2019.03119

Katalog-ID:	DOAJ064146294

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allfields	10.3389/fimmu.2019.03119 doi (DE-627)DOAJ064146294 (DE-599)DOAJ42d3ffe3e0c34dbf8cf6a7252d9c0ab8 DE-627 ger DE-627 rakwb eng RC581-607 Shima Hassan Baien verfasserin aut Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine. gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus Immunologic diseases. Allergy Shima Hassan Baien verfasserin aut Jana Seele verfasserin aut Jana Seele verfasserin aut Timo Henneck verfasserin aut Timo Henneck verfasserin aut Christin Freibrodt verfasserin aut Christin Freibrodt verfasserin aut György Szura verfasserin aut Hani Moubasher verfasserin aut Roland Nau verfasserin aut Roland Nau verfasserin aut Graham Brogden verfasserin aut Matthias Mörgelin verfasserin aut Mahavir Singh verfasserin aut Manfred Kietzmann verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Nicole de Buhr verfasserin aut Nicole de Buhr verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 10(2020) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:10 year:2020 https://doi.org/10.3389/fimmu.2019.03119 kostenfrei https://doaj.org/article/42d3ffe3e0c34dbf8cf6a7252d9c0ab8 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2019.03119/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020
spelling	10.3389/fimmu.2019.03119 doi (DE-627)DOAJ064146294 (DE-599)DOAJ42d3ffe3e0c34dbf8cf6a7252d9c0ab8 DE-627 ger DE-627 rakwb eng RC581-607 Shima Hassan Baien verfasserin aut Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine. gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus Immunologic diseases. Allergy Shima Hassan Baien verfasserin aut Jana Seele verfasserin aut Jana Seele verfasserin aut Timo Henneck verfasserin aut Timo Henneck verfasserin aut Christin Freibrodt verfasserin aut Christin Freibrodt verfasserin aut György Szura verfasserin aut Hani Moubasher verfasserin aut Roland Nau verfasserin aut Roland Nau verfasserin aut Graham Brogden verfasserin aut Matthias Mörgelin verfasserin aut Mahavir Singh verfasserin aut Manfred Kietzmann verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Nicole de Buhr verfasserin aut Nicole de Buhr verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 10(2020) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:10 year:2020 https://doi.org/10.3389/fimmu.2019.03119 kostenfrei https://doaj.org/article/42d3ffe3e0c34dbf8cf6a7252d9c0ab8 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2019.03119/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020
allfields_unstemmed	10.3389/fimmu.2019.03119 doi (DE-627)DOAJ064146294 (DE-599)DOAJ42d3ffe3e0c34dbf8cf6a7252d9c0ab8 DE-627 ger DE-627 rakwb eng RC581-607 Shima Hassan Baien verfasserin aut Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine. gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus Immunologic diseases. Allergy Shima Hassan Baien verfasserin aut Jana Seele verfasserin aut Jana Seele verfasserin aut Timo Henneck verfasserin aut Timo Henneck verfasserin aut Christin Freibrodt verfasserin aut Christin Freibrodt verfasserin aut György Szura verfasserin aut Hani Moubasher verfasserin aut Roland Nau verfasserin aut Roland Nau verfasserin aut Graham Brogden verfasserin aut Matthias Mörgelin verfasserin aut Mahavir Singh verfasserin aut Manfred Kietzmann verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Nicole de Buhr verfasserin aut Nicole de Buhr verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 10(2020) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:10 year:2020 https://doi.org/10.3389/fimmu.2019.03119 kostenfrei https://doaj.org/article/42d3ffe3e0c34dbf8cf6a7252d9c0ab8 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2019.03119/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020
allfieldsGer	10.3389/fimmu.2019.03119 doi (DE-627)DOAJ064146294 (DE-599)DOAJ42d3ffe3e0c34dbf8cf6a7252d9c0ab8 DE-627 ger DE-627 rakwb eng RC581-607 Shima Hassan Baien verfasserin aut Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine. gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus Immunologic diseases. Allergy Shima Hassan Baien verfasserin aut Jana Seele verfasserin aut Jana Seele verfasserin aut Timo Henneck verfasserin aut Timo Henneck verfasserin aut Christin Freibrodt verfasserin aut Christin Freibrodt verfasserin aut György Szura verfasserin aut Hani Moubasher verfasserin aut Roland Nau verfasserin aut Roland Nau verfasserin aut Graham Brogden verfasserin aut Matthias Mörgelin verfasserin aut Mahavir Singh verfasserin aut Manfred Kietzmann verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Nicole de Buhr verfasserin aut Nicole de Buhr verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 10(2020) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:10 year:2020 https://doi.org/10.3389/fimmu.2019.03119 kostenfrei https://doaj.org/article/42d3ffe3e0c34dbf8cf6a7252d9c0ab8 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2019.03119/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020
allfieldsSound	10.3389/fimmu.2019.03119 doi (DE-627)DOAJ064146294 (DE-599)DOAJ42d3ffe3e0c34dbf8cf6a7252d9c0ab8 DE-627 ger DE-627 rakwb eng RC581-607 Shima Hassan Baien verfasserin aut Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine. gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus Immunologic diseases. Allergy Shima Hassan Baien verfasserin aut Jana Seele verfasserin aut Jana Seele verfasserin aut Timo Henneck verfasserin aut Timo Henneck verfasserin aut Christin Freibrodt verfasserin aut Christin Freibrodt verfasserin aut György Szura verfasserin aut Hani Moubasher verfasserin aut Roland Nau verfasserin aut Roland Nau verfasserin aut Graham Brogden verfasserin aut Matthias Mörgelin verfasserin aut Mahavir Singh verfasserin aut Manfred Kietzmann verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Maren von Köckritz-Blickwede verfasserin aut Nicole de Buhr verfasserin aut Nicole de Buhr verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 10(2020) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:10 year:2020 https://doi.org/10.3389/fimmu.2019.03119 kostenfrei https://doaj.org/article/42d3ffe3e0c34dbf8cf6a7252d9c0ab8 kostenfrei https://www.frontiersin.org/article/10.3389/fimmu.2019.03119/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020
language	English
source	In Frontiers in Immunology 10(2020) volume:10 year:2020
sourceStr	In Frontiers in Immunology 10(2020) volume:10 year:2020
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus Immunologic diseases. Allergy
isfreeaccess_bool	true
container_title	Frontiers in Immunology
authorswithroles_txt_mv	Shima Hassan Baien @@aut@@ Jana Seele @@aut@@ Timo Henneck @@aut@@ Christin Freibrodt @@aut@@ György Szura @@aut@@ Hani Moubasher @@aut@@ Roland Nau @@aut@@ Graham Brogden @@aut@@ Matthias Mörgelin @@aut@@ Mahavir Singh @@aut@@ Manfred Kietzmann @@aut@@ Maren von Köckritz-Blickwede @@aut@@ Nicole de Buhr @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	657998354
id	DOAJ064146294
language_de	englisch
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callnumber-first	R - Medicine
author	Shima Hassan Baien
spellingShingle	Shima Hassan Baien misc RC581-607 misc gum arabic misc neutrophils misc immune modulation misc indirect effect misc direct effect misc Staphylococcus aureus misc Immunologic diseases. Allergy Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli
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topic_title	RC581-607 Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli gum arabic neutrophils immune modulation indirect effect direct effect Staphylococcus aureus
topic	misc RC581-607 misc gum arabic misc neutrophils misc immune modulation misc indirect effect misc direct effect misc Staphylococcus aureus misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc gum arabic misc neutrophils misc immune modulation misc indirect effect misc direct effect misc Staphylococcus aureus misc Immunologic diseases. Allergy
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title	Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli
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title_full	Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli
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author_browse	Shima Hassan Baien Jana Seele Timo Henneck Christin Freibrodt György Szura Hani Moubasher Roland Nau Graham Brogden Matthias Mörgelin Mahavir Singh Manfred Kietzmann Maren von Köckritz-Blickwede Nicole de Buhr
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title_sort	antimicrobial and immunomodulatory effect of gum arabic on human and bovine granulocytes against staphylococcus aureus and escherichia coli
callnumber	RC581-607
title_auth	Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli
abstract	Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine.
abstractGer	Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine.
abstract_unstemmed	Gum arabic (GA) is a traditional herbal medicine from Acacia Senegal (L.) Willdenow trees, which consist of a complex mixture of polysaccharides and glycoproteins. It is used in daily applications for several diseases and is considered to protect against bacterial infections. The detailed mechanisms behind these observations are still unclear. In this study, we investigated the direct antibacterial activity of GA water and ethanol extracts against Staphylococcus (S.) aureus or Escherichia (E.) coli and the immunomodulating properties of those extracts on granulocytes as a first line of defense against bacteria. Firstly, the direct antimicrobial effect of GA was tested on three different S. aureus strains and two E. coli strains. The growth of bacteria was analyzed in the presence of different GA concentrations over time. GA water as well as ethanol extracts showed a significant growth inhibition in a concentration-dependent manner in the case of S. aureus Newman, S. aureus Rd5, and E. coli 25922, but not in the case of S. aureus USA300 and E. coli K1. Transmission electron microscopic analysis confirmed an antibacterial effect of GA on the bacteria. Secondly, the immunomodulatory effect of GA on the antimicrobial activity of bovine or human blood-derived granulocytes was evaluated. Interestingly, water and ethanol extracts enhanced antimicrobial activity of granulocytes by the induction of intracellular ROS production. In line with these data, GA increased the phagocytosis rate of E. coli. No effect was seen on neutrophil extracellular trap (NET) formation that mediates killing of extracellular bacteria such as S. aureus. In conclusion, we show that GA exhibits a direct antibacterial effect against some S. aureus and E. coli strains. Furthermore, GA boosts the antimicrobial activities of granulocytes and increases intracellular ROS production, which may lead to more phagocytosis and intracellular killing. These data might explain the described putative antimicrobial activity of GA used in traditional medicine.
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title_short	Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli
url	https://doi.org/10.3389/fimmu.2019.03119 https://doaj.org/article/42d3ffe3e0c34dbf8cf6a7252d9c0ab8 https://www.frontiersin.org/article/10.3389/fimmu.2019.03119/full https://doaj.org/toc/1664-3224
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up_date	2024-07-03T21:25:08.817Z
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Antimicrobial and Immunomodulatory Effect of Gum Arabic on Human and Bovine Granulocytes Against Staphylococcus aureus and Escherichia coli

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