Isolation and characterization of peritoneal microvascular pericytes

As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we desc...
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Autor*in:	Lei Tang [verfasserIn] Jun Shi [verfasserIn] Manshu Yu [verfasserIn] Yun Shan [verfasserIn] Juan Zhao [verfasserIn] Meixiao Sheng [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis

Übergeordnetes Werk:	In: FEBS Open Bio - Wiley, 2013, 12(2022), 4, Seite 784-797
Übergeordnetes Werk:	volume:12 ; year:2022 ; number:4 ; pages:784-797

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1002/2211-5463.13386

Katalog-ID:	DOAJ064282546

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520			\|a As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies.
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allfields	10.1002/2211-5463.13386 doi (DE-627)DOAJ064282546 (DE-599)DOAJ571bde5e916a43ceac01e4963e1215a3 DE-627 ger DE-627 rakwb eng QH301-705.5 Lei Tang verfasserin aut Isolation and characterization of peritoneal microvascular pericytes 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies. isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis Biology (General) Jun Shi verfasserin aut Manshu Yu verfasserin aut Yun Shan verfasserin aut Juan Zhao verfasserin aut Meixiao Sheng verfasserin aut In FEBS Open Bio Wiley, 2013 12(2022), 4, Seite 784-797 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:12 year:2022 number:4 pages:784-797 https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/article/571bde5e916a43ceac01e4963e1215a3 kostenfrei https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 4 784-797
spelling	10.1002/2211-5463.13386 doi (DE-627)DOAJ064282546 (DE-599)DOAJ571bde5e916a43ceac01e4963e1215a3 DE-627 ger DE-627 rakwb eng QH301-705.5 Lei Tang verfasserin aut Isolation and characterization of peritoneal microvascular pericytes 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies. isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis Biology (General) Jun Shi verfasserin aut Manshu Yu verfasserin aut Yun Shan verfasserin aut Juan Zhao verfasserin aut Meixiao Sheng verfasserin aut In FEBS Open Bio Wiley, 2013 12(2022), 4, Seite 784-797 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:12 year:2022 number:4 pages:784-797 https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/article/571bde5e916a43ceac01e4963e1215a3 kostenfrei https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 4 784-797
allfields_unstemmed	10.1002/2211-5463.13386 doi (DE-627)DOAJ064282546 (DE-599)DOAJ571bde5e916a43ceac01e4963e1215a3 DE-627 ger DE-627 rakwb eng QH301-705.5 Lei Tang verfasserin aut Isolation and characterization of peritoneal microvascular pericytes 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies. isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis Biology (General) Jun Shi verfasserin aut Manshu Yu verfasserin aut Yun Shan verfasserin aut Juan Zhao verfasserin aut Meixiao Sheng verfasserin aut In FEBS Open Bio Wiley, 2013 12(2022), 4, Seite 784-797 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:12 year:2022 number:4 pages:784-797 https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/article/571bde5e916a43ceac01e4963e1215a3 kostenfrei https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 4 784-797
allfieldsGer	10.1002/2211-5463.13386 doi (DE-627)DOAJ064282546 (DE-599)DOAJ571bde5e916a43ceac01e4963e1215a3 DE-627 ger DE-627 rakwb eng QH301-705.5 Lei Tang verfasserin aut Isolation and characterization of peritoneal microvascular pericytes 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies. isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis Biology (General) Jun Shi verfasserin aut Manshu Yu verfasserin aut Yun Shan verfasserin aut Juan Zhao verfasserin aut Meixiao Sheng verfasserin aut In FEBS Open Bio Wiley, 2013 12(2022), 4, Seite 784-797 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:12 year:2022 number:4 pages:784-797 https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/article/571bde5e916a43ceac01e4963e1215a3 kostenfrei https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 4 784-797
allfieldsSound	10.1002/2211-5463.13386 doi (DE-627)DOAJ064282546 (DE-599)DOAJ571bde5e916a43ceac01e4963e1215a3 DE-627 ger DE-627 rakwb eng QH301-705.5 Lei Tang verfasserin aut Isolation and characterization of peritoneal microvascular pericytes 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies. isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis Biology (General) Jun Shi verfasserin aut Manshu Yu verfasserin aut Yun Shan verfasserin aut Juan Zhao verfasserin aut Meixiao Sheng verfasserin aut In FEBS Open Bio Wiley, 2013 12(2022), 4, Seite 784-797 (DE-627)686948351 (DE-600)2651702-4 22115463 nnns volume:12 year:2022 number:4 pages:784-797 https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/article/571bde5e916a43ceac01e4963e1215a3 kostenfrei https://doi.org/10.1002/2211-5463.13386 kostenfrei https://doaj.org/toc/2211-5463 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 4 784-797
language	English
source	In FEBS Open Bio 12(2022), 4, Seite 784-797 volume:12 year:2022 number:4 pages:784-797
sourceStr	In FEBS Open Bio 12(2022), 4, Seite 784-797 volume:12 year:2022 number:4 pages:784-797
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis Biology (General)
isfreeaccess_bool	true
container_title	FEBS Open Bio
authorswithroles_txt_mv	Lei Tang @@aut@@ Jun Shi @@aut@@ Manshu Yu @@aut@@ Yun Shan @@aut@@ Juan Zhao @@aut@@ Meixiao Sheng @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	686948351
id	DOAJ064282546
language_de	englisch
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callnumber-first	Q - Science
author	Lei Tang
spellingShingle	Lei Tang misc QH301-705.5 misc isolation and characterization misc pericyte–myofibroblast transition misc pericytes misc peritoneal fibrosis misc Biology (General) Isolation and characterization of peritoneal microvascular pericytes
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topic_title	QH301-705.5 Isolation and characterization of peritoneal microvascular pericytes isolation and characterization pericyte–myofibroblast transition pericytes peritoneal fibrosis
topic	misc QH301-705.5 misc isolation and characterization misc pericyte–myofibroblast transition misc pericytes misc peritoneal fibrosis misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc isolation and characterization misc pericyte–myofibroblast transition misc pericytes misc peritoneal fibrosis misc Biology (General)
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title	Isolation and characterization of peritoneal microvascular pericytes
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title_full	Isolation and characterization of peritoneal microvascular pericytes
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title_sort	isolation and characterization of peritoneal microvascular pericytes
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title_auth	Isolation and characterization of peritoneal microvascular pericytes
abstract	As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies.
abstractGer	As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies.
abstract_unstemmed	As a potential source of myofibroblasts, pericytes may play a role in human peritoneal fibrosis. The culture of primary vascular pericytes in animals has previously been reported, most of which are derived from cerebral and retinal microvasculature. Here, in the field of peritoneal dialysis, we describe a method to isolate and characterize mouse peritoneal microvascular pericytes. The mesenteric tissues of five mice were collected and digested by type II collagenase and type I DNase. After cell attachment, the culture fluid was replaced with pericyte‐conditioned medium. Pericytes with high purity (99.0%) could be isolated by enzymatic disaggregation combined with conditional culture and magnetic activated cell sorting. The primary cells were triangular or polygonal with protrusions, and confluent cell culture could be established in 3 days. The primary pericytes were positive for platelet‐derived growth factor receptor‐β, α‐smooth muscle actin, neuron‐glial antigen 2, and CD13. Moreover, they promoted formation of endothelial tubes, and pericyte–myofibroblast transition occurred after treatment with transforming growth factor‐β1. In summary, we describe here a reproducible isolation protocol for primary peritoneal pericytes, which may be a powerful tool for in vitro peritoneal fibrosis studies.
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title_short	Isolation and characterization of peritoneal microvascular pericytes
url	https://doi.org/10.1002/2211-5463.13386 https://doaj.org/article/571bde5e916a43ceac01e4963e1215a3 https://doaj.org/toc/2211-5463
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Isolation and characterization of peritoneal microvascular pericytes

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