Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treat...
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Autor*in:	Mohammad El Khatib [verfasserIn] Annunziata Mauro [verfasserIn] Ralf Wyrwa [verfasserIn] Miriam Di Mattia [verfasserIn] Maura Turriani [verfasserIn] Oriana Di Giacinto [verfasserIn] Björn Kretzschmar [verfasserIn] Thomas Seemann [verfasserIn] Luca Valbonetti [verfasserIn] Paolo Berardinelli [verfasserIn] Matthias Schnabelrauch [verfasserIn] Barbara Barboni [verfasserIn] Valentina Russo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 25(2020), 14, p 3176
Übergeordnetes Werk:	volume:25 ; year:2020 ; number:14, p 3176

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules25143176

Katalog-ID:	DOAJ064336689

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allfields	10.3390/molecules25143176 doi (DE-627)DOAJ064336689 (DE-599)DOAJ4c0ccd97babd4dfb9855149df372a732 DE-627 ger DE-627 rakwb eng QD241-441 Mohammad El Khatib verfasserin aut Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct. aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA Organic chemistry Annunziata Mauro verfasserin aut Ralf Wyrwa verfasserin aut Miriam Di Mattia verfasserin aut Maura Turriani verfasserin aut Oriana Di Giacinto verfasserin aut Björn Kretzschmar verfasserin aut Thomas Seemann verfasserin aut Luca Valbonetti verfasserin aut Paolo Berardinelli verfasserin aut Matthias Schnabelrauch verfasserin aut Barbara Barboni verfasserin aut Valentina Russo verfasserin aut In Molecules MDPI AG, 2003 25(2020), 14, p 3176 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:14, p 3176 https://doi.org/10.3390/molecules25143176 kostenfrei https://doaj.org/article/4c0ccd97babd4dfb9855149df372a732 kostenfrei https://www.mdpi.com/1420-3049/25/14/3176 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 14, p 3176
spelling	10.3390/molecules25143176 doi (DE-627)DOAJ064336689 (DE-599)DOAJ4c0ccd97babd4dfb9855149df372a732 DE-627 ger DE-627 rakwb eng QD241-441 Mohammad El Khatib verfasserin aut Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct. aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA Organic chemistry Annunziata Mauro verfasserin aut Ralf Wyrwa verfasserin aut Miriam Di Mattia verfasserin aut Maura Turriani verfasserin aut Oriana Di Giacinto verfasserin aut Björn Kretzschmar verfasserin aut Thomas Seemann verfasserin aut Luca Valbonetti verfasserin aut Paolo Berardinelli verfasserin aut Matthias Schnabelrauch verfasserin aut Barbara Barboni verfasserin aut Valentina Russo verfasserin aut In Molecules MDPI AG, 2003 25(2020), 14, p 3176 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:14, p 3176 https://doi.org/10.3390/molecules25143176 kostenfrei https://doaj.org/article/4c0ccd97babd4dfb9855149df372a732 kostenfrei https://www.mdpi.com/1420-3049/25/14/3176 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 14, p 3176
allfields_unstemmed	10.3390/molecules25143176 doi (DE-627)DOAJ064336689 (DE-599)DOAJ4c0ccd97babd4dfb9855149df372a732 DE-627 ger DE-627 rakwb eng QD241-441 Mohammad El Khatib verfasserin aut Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct. aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA Organic chemistry Annunziata Mauro verfasserin aut Ralf Wyrwa verfasserin aut Miriam Di Mattia verfasserin aut Maura Turriani verfasserin aut Oriana Di Giacinto verfasserin aut Björn Kretzschmar verfasserin aut Thomas Seemann verfasserin aut Luca Valbonetti verfasserin aut Paolo Berardinelli verfasserin aut Matthias Schnabelrauch verfasserin aut Barbara Barboni verfasserin aut Valentina Russo verfasserin aut In Molecules MDPI AG, 2003 25(2020), 14, p 3176 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:14, p 3176 https://doi.org/10.3390/molecules25143176 kostenfrei https://doaj.org/article/4c0ccd97babd4dfb9855149df372a732 kostenfrei https://www.mdpi.com/1420-3049/25/14/3176 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 14, p 3176
allfieldsGer	10.3390/molecules25143176 doi (DE-627)DOAJ064336689 (DE-599)DOAJ4c0ccd97babd4dfb9855149df372a732 DE-627 ger DE-627 rakwb eng QD241-441 Mohammad El Khatib verfasserin aut Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct. aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA Organic chemistry Annunziata Mauro verfasserin aut Ralf Wyrwa verfasserin aut Miriam Di Mattia verfasserin aut Maura Turriani verfasserin aut Oriana Di Giacinto verfasserin aut Björn Kretzschmar verfasserin aut Thomas Seemann verfasserin aut Luca Valbonetti verfasserin aut Paolo Berardinelli verfasserin aut Matthias Schnabelrauch verfasserin aut Barbara Barboni verfasserin aut Valentina Russo verfasserin aut In Molecules MDPI AG, 2003 25(2020), 14, p 3176 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:14, p 3176 https://doi.org/10.3390/molecules25143176 kostenfrei https://doaj.org/article/4c0ccd97babd4dfb9855149df372a732 kostenfrei https://www.mdpi.com/1420-3049/25/14/3176 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 14, p 3176
allfieldsSound	10.3390/molecules25143176 doi (DE-627)DOAJ064336689 (DE-599)DOAJ4c0ccd97babd4dfb9855149df372a732 DE-627 ger DE-627 rakwb eng QD241-441 Mohammad El Khatib verfasserin aut Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct. aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA Organic chemistry Annunziata Mauro verfasserin aut Ralf Wyrwa verfasserin aut Miriam Di Mattia verfasserin aut Maura Turriani verfasserin aut Oriana Di Giacinto verfasserin aut Björn Kretzschmar verfasserin aut Thomas Seemann verfasserin aut Luca Valbonetti verfasserin aut Paolo Berardinelli verfasserin aut Matthias Schnabelrauch verfasserin aut Barbara Barboni verfasserin aut Valentina Russo verfasserin aut In Molecules MDPI AG, 2003 25(2020), 14, p 3176 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:14, p 3176 https://doi.org/10.3390/molecules25143176 kostenfrei https://doaj.org/article/4c0ccd97babd4dfb9855149df372a732 kostenfrei https://www.mdpi.com/1420-3049/25/14/3176 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 14, p 3176
language	English
source	In Molecules 25(2020), 14, p 3176 volume:25 year:2020 number:14, p 3176
sourceStr	In Molecules 25(2020), 14, p 3176 volume:25 year:2020 number:14, p 3176
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA Organic chemistry
isfreeaccess_bool	true
container_title	Molecules
authorswithroles_txt_mv	Mohammad El Khatib @@aut@@ Annunziata Mauro @@aut@@ Ralf Wyrwa @@aut@@ Miriam Di Mattia @@aut@@ Maura Turriani @@aut@@ Oriana Di Giacinto @@aut@@ Björn Kretzschmar @@aut@@ Thomas Seemann @@aut@@ Luca Valbonetti @@aut@@ Paolo Berardinelli @@aut@@ Matthias Schnabelrauch @@aut@@ Barbara Barboni @@aut@@ Valentina Russo @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	311313132
id	DOAJ064336689
language_de	englisch
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callnumber-first	Q - Science
author	Mohammad El Khatib
spellingShingle	Mohammad El Khatib misc QD241-441 misc aligned microfibers misc amniotic epithelial stem cells misc cold atmospheric plasma misc electrospinning misc exposure time misc PLGA misc Organic chemistry Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential
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topic_title	QD241-441 Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential aligned microfibers amniotic epithelial stem cells cold atmospheric plasma electrospinning exposure time PLGA
topic	misc QD241-441 misc aligned microfibers misc amniotic epithelial stem cells misc cold atmospheric plasma misc electrospinning misc exposure time misc PLGA misc Organic chemistry
topic_unstemmed	misc QD241-441 misc aligned microfibers misc amniotic epithelial stem cells misc cold atmospheric plasma misc electrospinning misc exposure time misc PLGA misc Organic chemistry
topic_browse	misc QD241-441 misc aligned microfibers misc amniotic epithelial stem cells misc cold atmospheric plasma misc electrospinning misc exposure time misc PLGA misc Organic chemistry
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title	Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential
ctrlnum	(DE-627)DOAJ064336689 (DE-599)DOAJ4c0ccd97babd4dfb9855149df372a732
title_full	Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential
author_sort	Mohammad El Khatib
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author_browse	Mohammad El Khatib Annunziata Mauro Ralf Wyrwa Miriam Di Mattia Maura Turriani Oriana Di Giacinto Björn Kretzschmar Thomas Seemann Luca Valbonetti Paolo Berardinelli Matthias Schnabelrauch Barbara Barboni Valentina Russo
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author-letter	Mohammad El Khatib
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title_sort	fabrication and plasma surface activation of aligned electrospun plga fiber fleeces with improved adhesion and infiltration of amniotic epithelial stem cells maintaining their teno-inductive potential
callnumber	QD241-441
title_auth	Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential
abstract	Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct.
abstractGer	Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct.
abstract_unstemmed	Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct.
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title_short	Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential
url	https://doi.org/10.3390/molecules25143176 https://doaj.org/article/4c0ccd97babd4dfb9855149df372a732 https://www.mdpi.com/1420-3049/25/14/3176 https://doaj.org/toc/1420-3049
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up_date	2024-07-03T22:23:36.930Z
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Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

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