Yeast-Based Genetic Interaction Analysis of Human Kinome

Kinases are critical intracellular signaling proteins. To better understand kinase-mediated signal transduction, a large-scale human–yeast genetic interaction screen was performed. Among 597 human kinase genes tested, 28 displayed strong toxicity in yeast when overexpressed. <i<En masse</i&...
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Autor*in:	Jae-Hong Kim [verfasserIn] Yeojin Seo [verfasserIn] Myungjin Jo [verfasserIn] Hyejin Jeon [verfasserIn] Won-Ha Lee [verfasserIn] Nozomu Yachie [verfasserIn] Quan Zhong [verfasserIn] Marc Vidal [verfasserIn] Frederick P. Roth [verfasserIn] Kyoungho Suk [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	yeast kinase genetic interaction network bar-seq

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 9(2020), 5, p 1156
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:5, p 1156

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells9051156

Katalog-ID:	DOAJ064406431

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520			\|a Kinases are critical intracellular signaling proteins. To better understand kinase-mediated signal transduction, a large-scale human–yeast genetic interaction screen was performed. Among 597 human kinase genes tested, 28 displayed strong toxicity in yeast when overexpressed. <i<En masse</i< transformation of these toxic kinase genes into 4653 homozygous diploid yeast deletion mutants followed by barcode sequencing identified yeast toxicity modifiers and thus their human orthologs. Subsequent network analyses and functional grouping revealed that the 28 kinases and their 676 interaction partners (corresponding to a total of 969 genetic interactions) are enriched in cell death and survival (34%), small-molecule biochemistry (18%) and molecular transport (11%), among others. In the subnetwork analyses, a few kinases were commonly associated with glioma, cell migration and cell death/survival. Our analysis enabled the creation of a first draft of the kinase genetic interactome network and identified multiple drug targets for inflammatory diseases and cancer, in which deregulated kinase signaling plays a pathogenic role.
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allfieldsSound	10.3390/cells9051156 doi (DE-627)DOAJ064406431 (DE-599)DOAJf809bdb019f2413799fc4946e3bea2f7 DE-627 ger DE-627 rakwb eng QH573-671 Jae-Hong Kim verfasserin aut Yeast-Based Genetic Interaction Analysis of Human Kinome 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Kinases are critical intracellular signaling proteins. To better understand kinase-mediated signal transduction, a large-scale human–yeast genetic interaction screen was performed. Among 597 human kinase genes tested, 28 displayed strong toxicity in yeast when overexpressed. <i<En masse</i< transformation of these toxic kinase genes into 4653 homozygous diploid yeast deletion mutants followed by barcode sequencing identified yeast toxicity modifiers and thus their human orthologs. Subsequent network analyses and functional grouping revealed that the 28 kinases and their 676 interaction partners (corresponding to a total of 969 genetic interactions) are enriched in cell death and survival (34%), small-molecule biochemistry (18%) and molecular transport (11%), among others. In the subnetwork analyses, a few kinases were commonly associated with glioma, cell migration and cell death/survival. Our analysis enabled the creation of a first draft of the kinase genetic interactome network and identified multiple drug targets for inflammatory diseases and cancer, in which deregulated kinase signaling plays a pathogenic role. yeast kinase genetic interaction network bar-seq Cytology Yeojin Seo verfasserin aut Myungjin Jo verfasserin aut Hyejin Jeon verfasserin aut Won-Ha Lee verfasserin aut Nozomu Yachie verfasserin aut Quan Zhong verfasserin aut Marc Vidal verfasserin aut Frederick P. Roth verfasserin aut Kyoungho Suk verfasserin aut In Cells MDPI AG, 2012 9(2020), 5, p 1156 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:9 year:2020 number:5, p 1156 https://doi.org/10.3390/cells9051156 kostenfrei https://doaj.org/article/f809bdb019f2413799fc4946e3bea2f7 kostenfrei https://www.mdpi.com/2073-4409/9/5/1156 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 5, p 1156
language	English
source	In Cells 9(2020), 5, p 1156 volume:9 year:2020 number:5, p 1156
sourceStr	In Cells 9(2020), 5, p 1156 volume:9 year:2020 number:5, p 1156
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	yeast kinase genetic interaction network bar-seq Cytology
isfreeaccess_bool	true
container_title	Cells
authorswithroles_txt_mv	Jae-Hong Kim @@aut@@ Yeojin Seo @@aut@@ Myungjin Jo @@aut@@ Hyejin Jeon @@aut@@ Won-Ha Lee @@aut@@ Nozomu Yachie @@aut@@ Quan Zhong @@aut@@ Marc Vidal @@aut@@ Frederick P. Roth @@aut@@ Kyoungho Suk @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
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author	Jae-Hong Kim
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topic_title	QH573-671 Yeast-Based Genetic Interaction Analysis of Human Kinome yeast kinase genetic interaction network bar-seq
topic	misc QH573-671 misc yeast misc kinase misc genetic interaction misc network misc bar-seq misc Cytology
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title	Yeast-Based Genetic Interaction Analysis of Human Kinome
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title_auth	Yeast-Based Genetic Interaction Analysis of Human Kinome
abstract	Kinases are critical intracellular signaling proteins. To better understand kinase-mediated signal transduction, a large-scale human–yeast genetic interaction screen was performed. Among 597 human kinase genes tested, 28 displayed strong toxicity in yeast when overexpressed. <i<En masse</i< transformation of these toxic kinase genes into 4653 homozygous diploid yeast deletion mutants followed by barcode sequencing identified yeast toxicity modifiers and thus their human orthologs. Subsequent network analyses and functional grouping revealed that the 28 kinases and their 676 interaction partners (corresponding to a total of 969 genetic interactions) are enriched in cell death and survival (34%), small-molecule biochemistry (18%) and molecular transport (11%), among others. In the subnetwork analyses, a few kinases were commonly associated with glioma, cell migration and cell death/survival. Our analysis enabled the creation of a first draft of the kinase genetic interactome network and identified multiple drug targets for inflammatory diseases and cancer, in which deregulated kinase signaling plays a pathogenic role.
abstractGer	Kinases are critical intracellular signaling proteins. To better understand kinase-mediated signal transduction, a large-scale human–yeast genetic interaction screen was performed. Among 597 human kinase genes tested, 28 displayed strong toxicity in yeast when overexpressed. <i<En masse</i< transformation of these toxic kinase genes into 4653 homozygous diploid yeast deletion mutants followed by barcode sequencing identified yeast toxicity modifiers and thus their human orthologs. Subsequent network analyses and functional grouping revealed that the 28 kinases and their 676 interaction partners (corresponding to a total of 969 genetic interactions) are enriched in cell death and survival (34%), small-molecule biochemistry (18%) and molecular transport (11%), among others. In the subnetwork analyses, a few kinases were commonly associated with glioma, cell migration and cell death/survival. Our analysis enabled the creation of a first draft of the kinase genetic interactome network and identified multiple drug targets for inflammatory diseases and cancer, in which deregulated kinase signaling plays a pathogenic role.
abstract_unstemmed	Kinases are critical intracellular signaling proteins. To better understand kinase-mediated signal transduction, a large-scale human–yeast genetic interaction screen was performed. Among 597 human kinase genes tested, 28 displayed strong toxicity in yeast when overexpressed. <i<En masse</i< transformation of these toxic kinase genes into 4653 homozygous diploid yeast deletion mutants followed by barcode sequencing identified yeast toxicity modifiers and thus their human orthologs. Subsequent network analyses and functional grouping revealed that the 28 kinases and their 676 interaction partners (corresponding to a total of 969 genetic interactions) are enriched in cell death and survival (34%), small-molecule biochemistry (18%) and molecular transport (11%), among others. In the subnetwork analyses, a few kinases were commonly associated with glioma, cell migration and cell death/survival. Our analysis enabled the creation of a first draft of the kinase genetic interactome network and identified multiple drug targets for inflammatory diseases and cancer, in which deregulated kinase signaling plays a pathogenic role.
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title_short	Yeast-Based Genetic Interaction Analysis of Human Kinome
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Yeast-Based Genetic Interaction Analysis of Human Kinome

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