Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3

Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae...
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Autor*in:	Chen Wang [verfasserIn] Beifang Wang [verfasserIn] Liyong Cao [verfasserIn] Yingxin Zhang [verfasserIn] Yu Gao [verfasserIn] Yongrun Cao [verfasserIn] Yue Zhang [verfasserIn] Qunen Liu [verfasserIn] Xiaohui Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	rice LMM ZH8015 lm8015-3 defense

Übergeordnetes Werk:	In: Frontiers in Sustainable Food Systems - Frontiers Media S.A., 2018, 6(2022)
Übergeordnetes Werk:	volume:6 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fsufs.2022.809008

Katalog-ID:	DOAJ064534774

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520			\|a Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae (M. oryzae). Here, we identified a rice LMM, lm8015-3, which shows reduced resistance to M. oryzae. We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae–induced lm8015-3. Lm8015-3, obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H2O2, resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae, the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3. Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae. These results suggested that understanding how LMMs defend against M. oryzae infection will contribute to improving rice breeding.
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allfields	10.3389/fsufs.2022.809008 doi (DE-627)DOAJ064534774 (DE-599)DOAJ1029743919e64b1fb190ba5fc446490d DE-627 ger DE-627 rakwb eng TX341-641 TP368-456 Chen Wang verfasserin aut Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae (M. oryzae). Here, we identified a rice LMM, lm8015-3, which shows reduced resistance to M. oryzae. We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae–induced lm8015-3. Lm8015-3, obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H2O2, resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae, the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3. Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae. These results suggested that understanding how LMMs defend against M. oryzae infection will contribute to improving rice breeding. rice LMM ZH8015 lm8015-3 defense Nutrition. Foods and food supply Food processing and manufacture Beifang Wang verfasserin aut Liyong Cao verfasserin aut Liyong Cao verfasserin aut Yingxin Zhang verfasserin aut Yu Gao verfasserin aut Yongrun Cao verfasserin aut Yue Zhang verfasserin aut Qunen Liu verfasserin aut Xiaohui Zhang verfasserin aut Xiaohui Zhang verfasserin aut In Frontiers in Sustainable Food Systems Frontiers Media S.A., 2018 6(2022) (DE-627)1019902493 (DE-600)2928540-9 2571581X nnns volume:6 year:2022 https://doi.org/10.3389/fsufs.2022.809008 kostenfrei https://doaj.org/article/1029743919e64b1fb190ba5fc446490d kostenfrei https://www.frontiersin.org/articles/10.3389/fsufs.2022.809008/full kostenfrei https://doaj.org/toc/2571-581X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 6 2022
spelling	10.3389/fsufs.2022.809008 doi (DE-627)DOAJ064534774 (DE-599)DOAJ1029743919e64b1fb190ba5fc446490d DE-627 ger DE-627 rakwb eng TX341-641 TP368-456 Chen Wang verfasserin aut Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae (M. oryzae). Here, we identified a rice LMM, lm8015-3, which shows reduced resistance to M. oryzae. We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae–induced lm8015-3. Lm8015-3, obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H2O2, resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae, the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3. Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae. These results suggested that understanding how LMMs defend against M. oryzae infection will contribute to improving rice breeding. rice LMM ZH8015 lm8015-3 defense Nutrition. Foods and food supply Food processing and manufacture Beifang Wang verfasserin aut Liyong Cao verfasserin aut Liyong Cao verfasserin aut Yingxin Zhang verfasserin aut Yu Gao verfasserin aut Yongrun Cao verfasserin aut Yue Zhang verfasserin aut Qunen Liu verfasserin aut Xiaohui Zhang verfasserin aut Xiaohui Zhang verfasserin aut In Frontiers in Sustainable Food Systems Frontiers Media S.A., 2018 6(2022) (DE-627)1019902493 (DE-600)2928540-9 2571581X nnns volume:6 year:2022 https://doi.org/10.3389/fsufs.2022.809008 kostenfrei https://doaj.org/article/1029743919e64b1fb190ba5fc446490d kostenfrei https://www.frontiersin.org/articles/10.3389/fsufs.2022.809008/full kostenfrei https://doaj.org/toc/2571-581X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 6 2022
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allfieldsSound	10.3389/fsufs.2022.809008 doi (DE-627)DOAJ064534774 (DE-599)DOAJ1029743919e64b1fb190ba5fc446490d DE-627 ger DE-627 rakwb eng TX341-641 TP368-456 Chen Wang verfasserin aut Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae (M. oryzae). Here, we identified a rice LMM, lm8015-3, which shows reduced resistance to M. oryzae. We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae–induced lm8015-3. Lm8015-3, obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H2O2, resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae, the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3. Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae. These results suggested that understanding how LMMs defend against M. oryzae infection will contribute to improving rice breeding. rice LMM ZH8015 lm8015-3 defense Nutrition. Foods and food supply Food processing and manufacture Beifang Wang verfasserin aut Liyong Cao verfasserin aut Liyong Cao verfasserin aut Yingxin Zhang verfasserin aut Yu Gao verfasserin aut Yongrun Cao verfasserin aut Yue Zhang verfasserin aut Qunen Liu verfasserin aut Xiaohui Zhang verfasserin aut Xiaohui Zhang verfasserin aut In Frontiers in Sustainable Food Systems Frontiers Media S.A., 2018 6(2022) (DE-627)1019902493 (DE-600)2928540-9 2571581X nnns volume:6 year:2022 https://doi.org/10.3389/fsufs.2022.809008 kostenfrei https://doaj.org/article/1029743919e64b1fb190ba5fc446490d kostenfrei https://www.frontiersin.org/articles/10.3389/fsufs.2022.809008/full kostenfrei https://doaj.org/toc/2571-581X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 6 2022
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authorswithroles_txt_mv	Chen Wang @@aut@@ Beifang Wang @@aut@@ Liyong Cao @@aut@@ Yingxin Zhang @@aut@@ Yu Gao @@aut@@ Yongrun Cao @@aut@@ Yue Zhang @@aut@@ Qunen Liu @@aut@@ Xiaohui Zhang @@aut@@
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callnumber-first	T - Technology
author	Chen Wang
spellingShingle	Chen Wang misc TX341-641 misc TP368-456 misc rice misc LMM misc ZH8015 misc lm8015-3 misc defense misc Nutrition. Foods and food supply misc Food processing and manufacture Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3
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topic_title	TX341-641 TP368-456 Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3 rice LMM ZH8015 lm8015-3 defense
topic	misc TX341-641 misc TP368-456 misc rice misc LMM misc ZH8015 misc lm8015-3 misc defense misc Nutrition. Foods and food supply misc Food processing and manufacture
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title	Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3
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title_full	Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3
author_sort	Chen Wang
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author_browse	Chen Wang Beifang Wang Liyong Cao Yingxin Zhang Yu Gao Yongrun Cao Yue Zhang Qunen Liu Xiaohui Zhang
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title_sort	identification and gene mapping of the lesion mimic mutant lm8015-3
callnumber	TX341-641
title_auth	Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3
abstract	Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae (M. oryzae). Here, we identified a rice LMM, lm8015-3, which shows reduced resistance to M. oryzae. We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae–induced lm8015-3. Lm8015-3, obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H2O2, resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae, the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3. Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae. These results suggested that understanding how LMMs defend against M. oryzae infection will contribute to improving rice breeding.
abstractGer	Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae (M. oryzae). Here, we identified a rice LMM, lm8015-3, which shows reduced resistance to M. oryzae. We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae–induced lm8015-3. Lm8015-3, obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H2O2, resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae, the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3. Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae. These results suggested that understanding how LMMs defend against M. oryzae infection will contribute to improving rice breeding.
abstract_unstemmed	Lesion mimic mutants (LMMs) exhibit spots on leaves without fungal infection pressure. The spots confer variable resistance to pathogens in different LMM, making them useful research materials. It is unclear how the rice immune system responds to infection with the fungal pathogen Magnaporthe oryzae (M. oryzae). Here, we identified a rice LMM, lm8015-3, which shows reduced resistance to M. oryzae. We used Quantitative Real-Time PCR (qRT-PCR) to observe the immune system response to M. oryzae–induced lm8015-3. Lm8015-3, obtained from an ethyl methane sulfonate (EMS)–induced Zhonghui8015 (ZH8015) library, showed orange-yellow spots starting in the seedling stage and accumulated more H2O2, resulting in severe degradation of the chloroplast. With map-based cloning, the target gene was located on chromosome 12. Once inoculated with M. oryzae, the expression level of pathogen-related genes of lm8015-3 was downregulated between 48 and 72 h. In addition, more germinating spores appeared in lm8015-3. Therefore, we conclude that M. oryzae weakening the immune system of lm8015-3 from 48 to 72 h makes lm8015-3 more susceptible to M. oryzae. These results suggested that understanding how LMMs defend against M. oryzae infection will contribute to improving rice breeding.
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title_short	Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3
url	https://doi.org/10.3389/fsufs.2022.809008 https://doaj.org/article/1029743919e64b1fb190ba5fc446490d https://www.frontiersin.org/articles/10.3389/fsufs.2022.809008/full https://doaj.org/toc/2571-581X
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author2	Beifang Wang Liyong Cao Yingxin Zhang Yu Gao Yongrun Cao Yue Zhang Qunen Liu Xiaohui Zhang
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up_date	2024-07-03T23:20:17.743Z
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Identification and Gene Mapping of the Lesion Mimic Mutant lm8015-3

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