Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells
BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammato...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Jun Shu [verfasserIn] Xiaojuan He [verfasserIn] Lan Zhang [verfasserIn] Hong Li [verfasserIn] Ping Wang [verfasserIn] Xiaojie Huang [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
In: Biological Research - BMC, 2018 |
|---|
| Links: |
|---|
| DOI / URN: |
10.1186/s40659-015-0062-3 |
|---|
| Katalog-ID: |
DOAJ064747204 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | DOAJ064747204 | ||
| 003 | DE-627 | ||
| 005 | 20230309041846.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230228nuuuuuuuuxx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1186/s40659-015-0062-3 |2 doi | |
| 035 | |a (DE-627)DOAJ064747204 | ||
| 035 | |a (DE-599)DOAJ844a605201fc4e2695c1b612f64638ae | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 050 | 0 | |a QH301-705.5 | |
| 100 | 0 | |a Jun Shu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation | ||
| 650 | 4 | |a Human amnion mesenchymal cells | |
| 650 | 4 | |a THP-1 cells | |
| 650 | 4 | |a TNF-ZZZZZZZ | |
| 650 | 4 | |a IL-1XXXXXXXX | |
| 650 | 4 | |a Immunosuppression | |
| 653 | 0 | |a Biology (General) | |
| 700 | 0 | |a Xiaojuan He |e verfasserin |4 aut | |
| 700 | 0 | |a Lan Zhang |e verfasserin |4 aut | |
| 700 | 0 | |a Hong Li |e verfasserin |4 aut | |
| 700 | 0 | |a Ping Wang |e verfasserin |4 aut | |
| 700 | 0 | |a Xiaojie Huang |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t Biological Research |d BMC, 2018 |w (DE-627)329975374 |w (DE-600)2048380-6 |x 07176287 |7 nnns |
| 856 | 4 | 0 | |u https://doi.org/10.1186/s40659-015-0062-3 |z kostenfrei |
| 856 | 4 | 0 | |u https://doaj.org/article/844a605201fc4e2695c1b612f64638ae |z kostenfrei |
| 856 | 4 | 0 | |u http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/0716-9760 |y Journal toc |z kostenfrei |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_DOAJ | ||
| 951 | |a AR | ||
| author_variant |
j s js x h xh l z lz h l hl p w pw x h xh |
|---|---|
| matchkey_str |
article:07176287:uuuuuuuu::uaanomsnhmlelihbtiooyacaienuetfni |
| callnumber-subject-code |
QH |
| allfields |
10.1186/s40659-015-0062-3 doi (DE-627)DOAJ064747204 (DE-599)DOAJ844a605201fc4e2695c1b612f64638ae DE-627 ger DE-627 rakwb eng QH301-705.5 Jun Shu verfasserin aut Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation Human amnion mesenchymal cells THP-1 cells TNF-ZZZZZZZ IL-1XXXXXXXX Immunosuppression Biology (General) Xiaojuan He verfasserin aut Lan Zhang verfasserin aut Hong Li verfasserin aut Ping Wang verfasserin aut Xiaojie Huang verfasserin aut In Biological Research BMC, 2018 (DE-627)329975374 (DE-600)2048380-6 07176287 nnns https://doi.org/10.1186/s40659-015-0062-3 kostenfrei https://doaj.org/article/844a605201fc4e2695c1b612f64638ae kostenfrei http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en kostenfrei https://doaj.org/toc/0716-9760 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR |
| spelling |
10.1186/s40659-015-0062-3 doi (DE-627)DOAJ064747204 (DE-599)DOAJ844a605201fc4e2695c1b612f64638ae DE-627 ger DE-627 rakwb eng QH301-705.5 Jun Shu verfasserin aut Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation Human amnion mesenchymal cells THP-1 cells TNF-ZZZZZZZ IL-1XXXXXXXX Immunosuppression Biology (General) Xiaojuan He verfasserin aut Lan Zhang verfasserin aut Hong Li verfasserin aut Ping Wang verfasserin aut Xiaojie Huang verfasserin aut In Biological Research BMC, 2018 (DE-627)329975374 (DE-600)2048380-6 07176287 nnns https://doi.org/10.1186/s40659-015-0062-3 kostenfrei https://doaj.org/article/844a605201fc4e2695c1b612f64638ae kostenfrei http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en kostenfrei https://doaj.org/toc/0716-9760 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR |
| allfields_unstemmed |
10.1186/s40659-015-0062-3 doi (DE-627)DOAJ064747204 (DE-599)DOAJ844a605201fc4e2695c1b612f64638ae DE-627 ger DE-627 rakwb eng QH301-705.5 Jun Shu verfasserin aut Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation Human amnion mesenchymal cells THP-1 cells TNF-ZZZZZZZ IL-1XXXXXXXX Immunosuppression Biology (General) Xiaojuan He verfasserin aut Lan Zhang verfasserin aut Hong Li verfasserin aut Ping Wang verfasserin aut Xiaojie Huang verfasserin aut In Biological Research BMC, 2018 (DE-627)329975374 (DE-600)2048380-6 07176287 nnns https://doi.org/10.1186/s40659-015-0062-3 kostenfrei https://doaj.org/article/844a605201fc4e2695c1b612f64638ae kostenfrei http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en kostenfrei https://doaj.org/toc/0716-9760 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR |
| allfieldsGer |
10.1186/s40659-015-0062-3 doi (DE-627)DOAJ064747204 (DE-599)DOAJ844a605201fc4e2695c1b612f64638ae DE-627 ger DE-627 rakwb eng QH301-705.5 Jun Shu verfasserin aut Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation Human amnion mesenchymal cells THP-1 cells TNF-ZZZZZZZ IL-1XXXXXXXX Immunosuppression Biology (General) Xiaojuan He verfasserin aut Lan Zhang verfasserin aut Hong Li verfasserin aut Ping Wang verfasserin aut Xiaojie Huang verfasserin aut In Biological Research BMC, 2018 (DE-627)329975374 (DE-600)2048380-6 07176287 nnns https://doi.org/10.1186/s40659-015-0062-3 kostenfrei https://doaj.org/article/844a605201fc4e2695c1b612f64638ae kostenfrei http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en kostenfrei https://doaj.org/toc/0716-9760 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR |
| allfieldsSound |
10.1186/s40659-015-0062-3 doi (DE-627)DOAJ064747204 (DE-599)DOAJ844a605201fc4e2695c1b612f64638ae DE-627 ger DE-627 rakwb eng QH301-705.5 Jun Shu verfasserin aut Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation Human amnion mesenchymal cells THP-1 cells TNF-ZZZZZZZ IL-1XXXXXXXX Immunosuppression Biology (General) Xiaojuan He verfasserin aut Lan Zhang verfasserin aut Hong Li verfasserin aut Ping Wang verfasserin aut Xiaojie Huang verfasserin aut In Biological Research BMC, 2018 (DE-627)329975374 (DE-600)2048380-6 07176287 nnns https://doi.org/10.1186/s40659-015-0062-3 kostenfrei https://doaj.org/article/844a605201fc4e2695c1b612f64638ae kostenfrei http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en kostenfrei https://doaj.org/toc/0716-9760 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR |
| language |
English |
| source |
In Biological Research |
| sourceStr |
In Biological Research |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Human amnion mesenchymal cells THP-1 cells TNF-ZZZZZZZ IL-1XXXXXXXX Immunosuppression Biology (General) |
| isfreeaccess_bool |
true |
| container_title |
Biological Research |
| authorswithroles_txt_mv |
Jun Shu @@aut@@ Xiaojuan He @@aut@@ Lan Zhang @@aut@@ Hong Li @@aut@@ Ping Wang @@aut@@ Xiaojie Huang @@aut@@ |
| publishDateDaySort_date |
2024-01-01T00:00:00Z |
| hierarchy_top_id |
329975374 |
| id |
DOAJ064747204 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ064747204</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309041846.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228nuuuuuuuuxx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s40659-015-0062-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ064747204</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ844a605201fc4e2695c1b612f64638ae</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Jun Shu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human amnion mesenchymal cells</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">THP-1 cells</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">TNF-ZZZZZZZ</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">IL-1XXXXXXXX</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunosuppression</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xiaojuan He</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Lan Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hong Li</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ping Wang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xiaojie Huang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Biological Research</subfield><subfield code="d">BMC, 2018</subfield><subfield code="w">(DE-627)329975374</subfield><subfield code="w">(DE-600)2048380-6</subfield><subfield code="x">07176287</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1186/s40659-015-0062-3</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/844a605201fc4e2695c1b612f64638ae</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/0716-9760</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield></record></collection>
|
| callnumber-first |
Q - Science |
| author |
Jun Shu |
| spellingShingle |
Jun Shu misc QH301-705.5 misc Human amnion mesenchymal cells misc THP-1 cells misc TNF-ZZZZZZZ misc IL-1XXXXXXXX misc Immunosuppression misc Biology (General) Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells |
| authorStr |
Jun Shu |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)329975374 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut aut aut |
| collection |
DOAJ |
| remote_str |
true |
| callnumber-label |
QH301-705 |
| illustrated |
Not Illustrated |
| issn |
07176287 |
| topic_title |
QH301-705.5 Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells Human amnion mesenchymal cells THP-1 cells TNF-ZZZZZZZ IL-1XXXXXXXX Immunosuppression |
| topic |
misc QH301-705.5 misc Human amnion mesenchymal cells misc THP-1 cells misc TNF-ZZZZZZZ misc IL-1XXXXXXXX misc Immunosuppression misc Biology (General) |
| topic_unstemmed |
misc QH301-705.5 misc Human amnion mesenchymal cells misc THP-1 cells misc TNF-ZZZZZZZ misc IL-1XXXXXXXX misc Immunosuppression misc Biology (General) |
| topic_browse |
misc QH301-705.5 misc Human amnion mesenchymal cells misc THP-1 cells misc TNF-ZZZZZZZ misc IL-1XXXXXXXX misc Immunosuppression misc Biology (General) |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
Biological Research |
| hierarchy_parent_id |
329975374 |
| hierarchy_top_title |
Biological Research |
| isfreeaccess_txt |
true |
| familylinks_str_mv |
(DE-627)329975374 (DE-600)2048380-6 |
| title |
Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells |
| ctrlnum |
(DE-627)DOAJ064747204 (DE-599)DOAJ844a605201fc4e2695c1b612f64638ae |
| title_full |
Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells |
| author_sort |
Jun Shu |
| journal |
Biological Research |
| journalStr |
Biological Research |
| callnumber-first-code |
Q |
| lang_code |
eng |
| isOA_bool |
true |
| recordtype |
marc |
| contenttype_str_mv |
txt |
| author_browse |
Jun Shu Xiaojuan He Lan Zhang Hong Li Ping Wang Xiaojie Huang |
| class |
QH301-705.5 |
| format_se |
Elektronische Aufsätze |
| author-letter |
Jun Shu |
| doi_str_mv |
10.1186/s40659-015-0062-3 |
| author2-role |
verfasserin |
| title_sort |
human amnion mesenchymal cells inhibit lipopolysaccharide-induced tnf-α and il-1β production in thp-1 cells |
| callnumber |
QH301-705.5 |
| title_auth |
Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells |
| abstract |
BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation |
| abstractGer |
BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation |
| abstract_unstemmed |
BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ |
| title_short |
Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells |
| url |
https://doi.org/10.1186/s40659-015-0062-3 https://doaj.org/article/844a605201fc4e2695c1b612f64638ae http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en https://doaj.org/toc/0716-9760 |
| remote_bool |
true |
| author2 |
Xiaojuan He Lan Zhang Hong Li Ping Wang Xiaojie Huang |
| author2Str |
Xiaojuan He Lan Zhang Hong Li Ping Wang Xiaojie Huang |
| ppnlink |
329975374 |
| callnumber-subject |
QH - Natural History and Biology |
| mediatype_str_mv |
c |
| isOA_txt |
true |
| hochschulschrift_bool |
false |
| doi_str |
10.1186/s40659-015-0062-3 |
| callnumber-a |
QH301-705.5 |
| up_date |
2024-07-04T00:12:23.911Z |
| _version_ |
1803605187139469312 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ064747204</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309041846.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228nuuuuuuuuxx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s40659-015-0062-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ064747204</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ844a605201fc4e2695c1b612f64638ae</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Jun Shu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1β production in THP-1 cells</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS RESULTS: The levels of TNF-α and IL-1β secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1β in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1β were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1β production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1β were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1β secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human amnion mesenchymal cells</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">THP-1 cells</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">TNF-ZZZZZZZ</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">IL-1XXXXXXXX</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunosuppression</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xiaojuan He</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Lan Zhang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hong Li</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Ping Wang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Xiaojie Huang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Biological Research</subfield><subfield code="d">BMC, 2018</subfield><subfield code="w">(DE-627)329975374</subfield><subfield code="w">(DE-600)2048380-6</subfield><subfield code="x">07176287</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1186/s40659-015-0062-3</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/844a605201fc4e2695c1b612f64638ae</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100069&lng=en&tlng=en</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/0716-9760</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield></record></collection>
|
| score |
7.4019384 |