The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis

<p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Hua Dasong [verfasserIn] Shao Jiaofang [verfasserIn] Yu Wei [verfasserIn] Li Lisha [verfasserIn] Yoon Jae-Geun [verfasserIn] Fang Xuefeng [verfasserIn] Zheng Shu [verfasserIn] Hood Leroy [verfasserIn] Goodlett David R [verfasserIn] Foltz Gregory [verfasserIn] Lin Biaoyang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Übergeordnetes Werk:	In: BMC Genomics - BMC, 2003, 12(2011), 1, p 11
Übergeordnetes Werk:	volume:12 ; year:2011 ; number:1, p 11

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2164-12-11

Katalog-ID:	DOAJ064947254

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ064947254
003	DE-627
005	20230309043355.0
007	cr uuu---uuuuu
008	230228s2011 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1186/1471-2164-12-11 \|2 doi
035			\|a (DE-627)DOAJ064947254
035			\|a (DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a TP248.13-248.65
050		0	\|a QH426-470
100	0		\|a Hua Dasong \|e verfasserin \|4 aut
245	1	4	\|a The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis
264		1	\|c 2011
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a <p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p<
653		0	\|a Biotechnology
653		0	\|a Genetics
700	0		\|a Shao Jiaofang \|e verfasserin \|4 aut
700	0		\|a Yu Wei \|e verfasserin \|4 aut
700	0		\|a Li Lisha \|e verfasserin \|4 aut
700	0		\|a Yoon Jae-Geun \|e verfasserin \|4 aut
700	0		\|a Fang Xuefeng \|e verfasserin \|4 aut
700	0		\|a Zheng Shu \|e verfasserin \|4 aut
700	0		\|a Hood Leroy \|e verfasserin \|4 aut
700	0		\|a Goodlett David R \|e verfasserin \|4 aut
700	0		\|a Foltz Gregory \|e verfasserin \|4 aut
700	0		\|a Lin Biaoyang \|e verfasserin \|4 aut
773	0	8	\|i In \|t BMC Genomics \|d BMC, 2003 \|g 12(2011), 1, p 11 \|w (DE-627)326644954 \|w (DE-600)2041499-7 \|x 14712164 \|7 nnns
773	1	8	\|g volume:12 \|g year:2011 \|g number:1, p 11
856	4	0	\|u https://doi.org/10.1186/1471-2164-12-11 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4 \|z kostenfrei
856	4	0	\|u http://www.biomedcentral.com/1471-2164/12/11 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1471-2164 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 12 \|j 2011 \|e 1, p 11

Indexfelder

author_variant	h d hd s j sj y w yw l l ll y j g yjg f x fx z s zs h l hl g d r gdr f g fg l b lb
matchkey_str	article:14712164:2011----::hisxirsospormnlolsoautfrennertdhpeepesom
hierarchy_sort_str	2011
callnumber-subject-code	TP
publishDate	2011
allfields	10.1186/1471-2164-12-11 doi (DE-627)DOAJ064947254 (DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hua Dasong verfasserin aut The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p< Biotechnology Genetics Shao Jiaofang verfasserin aut Yu Wei verfasserin aut Li Lisha verfasserin aut Yoon Jae-Geun verfasserin aut Fang Xuefeng verfasserin aut Zheng Shu verfasserin aut Hood Leroy verfasserin aut Goodlett David R verfasserin aut Foltz Gregory verfasserin aut Lin Biaoyang verfasserin aut In BMC Genomics BMC, 2003 12(2011), 1, p 11 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:12 year:2011 number:1, p 11 https://doi.org/10.1186/1471-2164-12-11 kostenfrei https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4 kostenfrei http://www.biomedcentral.com/1471-2164/12/11 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2011 1, p 11
spelling	10.1186/1471-2164-12-11 doi (DE-627)DOAJ064947254 (DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hua Dasong verfasserin aut The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p< Biotechnology Genetics Shao Jiaofang verfasserin aut Yu Wei verfasserin aut Li Lisha verfasserin aut Yoon Jae-Geun verfasserin aut Fang Xuefeng verfasserin aut Zheng Shu verfasserin aut Hood Leroy verfasserin aut Goodlett David R verfasserin aut Foltz Gregory verfasserin aut Lin Biaoyang verfasserin aut In BMC Genomics BMC, 2003 12(2011), 1, p 11 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:12 year:2011 number:1, p 11 https://doi.org/10.1186/1471-2164-12-11 kostenfrei https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4 kostenfrei http://www.biomedcentral.com/1471-2164/12/11 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2011 1, p 11
allfields_unstemmed	10.1186/1471-2164-12-11 doi (DE-627)DOAJ064947254 (DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hua Dasong verfasserin aut The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p< Biotechnology Genetics Shao Jiaofang verfasserin aut Yu Wei verfasserin aut Li Lisha verfasserin aut Yoon Jae-Geun verfasserin aut Fang Xuefeng verfasserin aut Zheng Shu verfasserin aut Hood Leroy verfasserin aut Goodlett David R verfasserin aut Foltz Gregory verfasserin aut Lin Biaoyang verfasserin aut In BMC Genomics BMC, 2003 12(2011), 1, p 11 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:12 year:2011 number:1, p 11 https://doi.org/10.1186/1471-2164-12-11 kostenfrei https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4 kostenfrei http://www.biomedcentral.com/1471-2164/12/11 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2011 1, p 11
allfieldsGer	10.1186/1471-2164-12-11 doi (DE-627)DOAJ064947254 (DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hua Dasong verfasserin aut The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p< Biotechnology Genetics Shao Jiaofang verfasserin aut Yu Wei verfasserin aut Li Lisha verfasserin aut Yoon Jae-Geun verfasserin aut Fang Xuefeng verfasserin aut Zheng Shu verfasserin aut Hood Leroy verfasserin aut Goodlett David R verfasserin aut Foltz Gregory verfasserin aut Lin Biaoyang verfasserin aut In BMC Genomics BMC, 2003 12(2011), 1, p 11 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:12 year:2011 number:1, p 11 https://doi.org/10.1186/1471-2164-12-11 kostenfrei https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4 kostenfrei http://www.biomedcentral.com/1471-2164/12/11 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2011 1, p 11
allfieldsSound	10.1186/1471-2164-12-11 doi (DE-627)DOAJ064947254 (DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Hua Dasong verfasserin aut The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p< Biotechnology Genetics Shao Jiaofang verfasserin aut Yu Wei verfasserin aut Li Lisha verfasserin aut Yoon Jae-Geun verfasserin aut Fang Xuefeng verfasserin aut Zheng Shu verfasserin aut Hood Leroy verfasserin aut Goodlett David R verfasserin aut Foltz Gregory verfasserin aut Lin Biaoyang verfasserin aut In BMC Genomics BMC, 2003 12(2011), 1, p 11 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:12 year:2011 number:1, p 11 https://doi.org/10.1186/1471-2164-12-11 kostenfrei https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4 kostenfrei http://www.biomedcentral.com/1471-2164/12/11 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2011 1, p 11
language	English
source	In BMC Genomics 12(2011), 1, p 11 volume:12 year:2011 number:1, p 11
sourceStr	In BMC Genomics 12(2011), 1, p 11 volume:12 year:2011 number:1, p 11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biotechnology Genetics
isfreeaccess_bool	true
container_title	BMC Genomics
authorswithroles_txt_mv	Hua Dasong @@aut@@ Shao Jiaofang @@aut@@ Yu Wei @@aut@@ Li Lisha @@aut@@ Yoon Jae-Geun @@aut@@ Fang Xuefeng @@aut@@ Zheng Shu @@aut@@ Hood Leroy @@aut@@ Goodlett David R @@aut@@ Foltz Gregory @@aut@@ Lin Biaoyang @@aut@@
publishDateDaySort_date	2011-01-01T00:00:00Z
hierarchy_top_id	326644954
id	DOAJ064947254
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ064947254</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309043355.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2011 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1471-2164-12-11</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ064947254</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">TP248.13-248.65</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH426-470</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Hua Dasong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biotechnology</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Genetics</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Shao Jiaofang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yu Wei</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Li Lisha</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yoon Jae-Geun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Fang Xuefeng</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Zheng Shu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hood Leroy</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Goodlett David R</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Foltz Gregory</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Lin Biaoyang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">BMC Genomics</subfield><subfield code="d">BMC, 2003</subfield><subfield code="g">12(2011), 1, p 11</subfield><subfield code="w">(DE-627)326644954</subfield><subfield code="w">(DE-600)2041499-7</subfield><subfield code="x">14712164</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:12</subfield><subfield code="g">year:2011</subfield><subfield code="g">number:1, p 11</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1186/1471-2164-12-11</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.biomedcentral.com/1471-2164/12/11</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1471-2164</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">12</subfield><subfield code="j">2011</subfield><subfield code="e">1, p 11</subfield></datafield></record></collection>
callnumber-first	T - Technology
author	Hua Dasong
spellingShingle	Hua Dasong misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis
authorStr	Hua Dasong
ppnlink_with_tag_str_mv	@@773@@(DE-627)326644954
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	TP248
illustrated	Not Illustrated
issn	14712164
topic_title	TP248.13-248.65 QH426-470 The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis
topic	misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics
topic_unstemmed	misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics
topic_browse	misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	BMC Genomics
hierarchy_parent_id	326644954
hierarchy_top_title	BMC Genomics
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)326644954 (DE-600)2041499-7
title	The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis
ctrlnum	(DE-627)DOAJ064947254 (DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4
title_full	The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis
author_sort	Hua Dasong
journal	BMC Genomics
journalStr	BMC Genomics
callnumber-first-code	T
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2011
contenttype_str_mv	txt
author_browse	Hua Dasong Shao Jiaofang Yu Wei Li Lisha Yoon Jae-Geun Fang Xuefeng Zheng Shu Hood Leroy Goodlett David R Foltz Gregory Lin Biaoyang
container_volume	12
class	TP248.13-248.65 QH426-470
format_se	Elektronische Aufsätze
author-letter	Hua Dasong
doi_str_mv	10.1186/1471-2164-12-11
author2-role	verfasserin
title_sort	<it<sox2 </it<response program in glioblastoma multiforme: an integrated chip-seq, expression microarray, and microrna analysis
callnumber	TP248.13-248.65
title_auth	The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis
abstract	<p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p<
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	1, p 11
title_short	The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis
url	https://doi.org/10.1186/1471-2164-12-11 https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4 http://www.biomedcentral.com/1471-2164/12/11 https://doaj.org/toc/1471-2164
remote_bool	true
author2	Shao Jiaofang Yu Wei Li Lisha Yoon Jae-Geun Fang Xuefeng Zheng Shu Hood Leroy Goodlett David R Foltz Gregory Lin Biaoyang
author2Str	Shao Jiaofang Yu Wei Li Lisha Yoon Jae-Geun Fang Xuefeng Zheng Shu Hood Leroy Goodlett David R Foltz Gregory Lin Biaoyang
ppnlink	326644954
callnumber-subject	TP - Chemical Technology
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.1186/1471-2164-12-11
callnumber-a	TP248.13-248.65
up_date	2024-07-04T00:56:38.222Z
_version_	1803607970390474752
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ064947254</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309043355.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2011 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/1471-2164-12-11</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ064947254</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJb071a35598f544feaf4d4d2f666a60b4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">TP248.13-248.65</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH426-470</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Hua Dasong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a"><p<Abstract</p< <p<Background</p< <p<<it<SOX2 </it<is a key gene implicated in maintaining the stemness of embryonic and adult stem cells. <it<SOX2 </it<appears to re-activate in several human cancers including glioblastoma multiforme (GBM), however, the detailed response program of <it<SOX2 </it<in GBM has not yet been defined.</p< <p<Results</p< <p<We show that knockdown of the <it<SOX2 </it<gene in LN229 GBM cells reduces cell proliferation and colony formation. We then comprehensively characterize the <it<SOX2 </it<response program by an integrated analysis using several advanced genomic technologies including ChIP-seq, microarray profiling, and microRNA sequencing. Using ChIP-seq technology, we identified 4883 <it<SOX2 </it<binding regions in the GBM cancer genome. <it<SOX2 </it<binding regions contain the consensus sequence wwTGnwTw that occurred 3931 instances in 2312 <it<SOX2 </it<binding regions. Microarray analysis identified 489 genes whose expression altered in response to <it<SOX2 </it<knockdown. Interesting findings include that <it<SOX2 </it<regulates the expression of SOX family proteins <it<SOX1 </it<and <it<SOX18</it<, and that <it<SOX2 </it<down regulates <it<BEX1 </it<(brain expressed X-linked 1) and <it<BEX2 </it<(brain expressed X-linked 2), two genes with tumor suppressor activity in GBM. Using next generation sequencing, we identified 105 precursor microRNAs (corresponding to 95 mature miRNAs) regulated by <it<SOX2</it<, including down regulation of miR-143, -145, -253-5p and miR-452. We also show that miR-145 and <it<SOX2 </it<form a double negative feedback loop in GBM cells, potentially creating a bistable system in GBM cells.</p< <p<Conclusions</p< <p<We present an integrated dataset of ChIP-seq, expression microarrays and microRNA sequencing representing the <it<SOX2 </it<response program in LN229 GBM cells. The insights gained from our integrated analysis further our understanding of the potential actions of <it<SOX2 </it<in carcinogenesis and serves as a useful resource for the research community.</p<</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biotechnology</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Genetics</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Shao Jiaofang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yu Wei</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Li Lisha</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Yoon Jae-Geun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Fang Xuefeng</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Zheng Shu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hood Leroy</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Goodlett David R</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Foltz Gregory</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Lin Biaoyang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">BMC Genomics</subfield><subfield code="d">BMC, 2003</subfield><subfield code="g">12(2011), 1, p 11</subfield><subfield code="w">(DE-627)326644954</subfield><subfield code="w">(DE-600)2041499-7</subfield><subfield code="x">14712164</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:12</subfield><subfield code="g">year:2011</subfield><subfield code="g">number:1, p 11</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1186/1471-2164-12-11</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/b071a35598f544feaf4d4d2f666a60b4</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.biomedcentral.com/1471-2164/12/11</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1471-2164</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">12</subfield><subfield code="j">2011</subfield><subfield code="e">1, p 11</subfield></datafield></record></collection>
score	7.400236

Nicht das Richtige dabei?

Schreiben Sie uns!

The <it<SOX2 </it<response program in glioblastoma multiforme: an integrated ChIP-seq, expression microarray, and microRNA analysis

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?